{"title":"Cell and Tissue Preservation for Single-Cell Research","description":"\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003cstrong\u003eCell and Tissue Preservation for Single-Cell Research\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003eThe interval between sample collection and cell capture is where most viability losses happen — not in the library prep, not on the sequencer. This collection covers that gap.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003eSeven preservation products, each built for a different point in the pre-analytical workflow:\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003cstrong\u003eShort-term tissue storage at 4°C\u003c\/strong\u003e The Animal Tissue Storage Kit keeps whole tissue viable for up to 72 hours at 2–8°C. Covers the window between surgical collection and dissociation — suitable for transport between sites, batched scheduling, and over 100 tissue types including brain, liver, lung, tumor, and reproductive tissue. Organ-specific variants are available for gastrointestinal tissue and pancreas, where standard storage solutions fall short of clinical collection requirements.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003cstrong\u003eLong-term cryopreservation of whole tissue\u003c\/strong\u003e The Animal Tissue Freezing Kit uses gradient-based cryoprotection to slow membrane damage during the freeze-down process — the step where conventional snap-freezing typically loses 10–30% of viable cells to ice crystal formation and osmotic stress. Supports –80°C and liquid nitrogen storage.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003cstrong\u003eCell suspension freezing\u003c\/strong\u003e Three options for suspensions post-dissociation: the Universal Cell Freezing Kit for general use, a Serum-Free One-Step Medium for streamlined processing, and a Serum-Free \/ DMSO-Free Medium for workflows where downstream assay compatibility rules out standard cryoprotectants. All support –80°C and liquid nitrogen.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003eAll products are tested end-to-end in scRNA-seq projects, with viability rates and captured cell counts from real sequencing runs — not just storage benchmarks.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003eCompatible with 10x Genomics Chromium, BD Rhapsody, and Drop-seq workflows.\u003c\/p\u003e","products":[{"product_id":"animal-tissue-storage-kit-fg-ba3301","title":"FireGene Animal Tissue Storage Kit - Preserves Viability \u0026 Integrity","description":"\u003ch3 id=\"overview\"\u003eOverview\u003c\/h3\u003e\n\u003cp\u003eThe\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eFireGene Animal Tissue Storage Kit\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eis designed to maintain tissue viability and structural integrity during storage. Ideal for applications like single-cell sequencing, organoid culture, and disease modeling, it ensures that tissue samples remain biologically relevant and analytically reliable after preservation.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch3 id=\"background-information\"\u003eBackground Information\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eSupports research and clinical workflows\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eby preserving the\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ephysiological condition\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eof tissue samples.\u003c\/li\u003e\n\u003cli\u003eEnables long-term storage of animal tissues for:\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eSingle-cell analysis\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003ewhere intact cells are essential.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eOrganoid culture\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003erequiring high-quality viable tissues.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiomarker discovery\u003c\/strong\u003e, drug screening, and histological studies.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003eUsed in:\n\u003cul\u003e\n\u003cli\u003eDisease mechanism exploration.\u003c\/li\u003e\n\u003cli\u003eToxicity testing in drug development.\u003c\/li\u003e\n\u003cli\u003eDiagnostic sample preservation in clinical pathology.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch3 id=\"detection-principle\"\u003eDetection Principle\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eThe solution is formulated to:\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eMaintain osmotic and metabolic balance\u003c\/strong\u003e, preventing swelling\/shrinkage.\u003c\/li\u003e\n\u003cli\u003eInclude\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eantioxidants\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eto reduce oxidative damage from reactive oxygen species.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eInhibit apoptotic\/necrotic pathways\u003c\/strong\u003e, minimizing cell death during storage.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003eThe result:\n\u003cul\u003e\n\u003cli\u003eHigh-integrity tissue samples ready for downstream applications like\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003escRNA-seq\u003c\/strong\u003e, immunostaining, or culture.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 id=\"detection-principle\"\u003eSpecifications\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eSingle-cell sequencing, cell culture or other cell-related detections\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eCompatible Sample Types\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eAnimal tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eSupported Instruments\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e\/\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e4 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eShelf-life\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 id=\"detection-principle\"\u003eKit Components\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e100ml\/kit\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 60%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003e100 mL\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003eATS\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e500ml\/kit\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 60%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003e500 mL\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003eATS\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003c\/h3\u003e\n\u003ch3 id=\"detection-principle\"\u003eProduct FAQ\u003c\/h3\u003e\n\u003ch4\u003e1. Q1: What is the main purpose of FG-BA3301 Animal Tissue Universal Preservation Solution? And in which experimental scenarios can it be applied?\u003c\/h4\u003e\n\u003cp\u003e\u003cspan\u003eA: This product provides a simple, safe and effective method for preserving isolated animal tissues. It can well maintain the activity of cells in isolated tissues and solve the problem of preserving and transporting isolated tissue samples after collection. It can be widely used in high-throughput single-cell sequencing and other related scientific research experiments.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eIt can be used for tissue transportation, preservation, tissue digestion, and subsequently for cell culture, genome and transcriptome extraction, cell differentiation, etc.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch4\u003e2. Q2: What are the product's item number and volume of this preservation solution?\u003c\/h4\u003e\n\u003cp\u003e\u003cspan\u003eA: Its only component is the animal tissue universal preservation solution, with the item number FG-BA3301 and a volume of 100mL.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch4\u003e3. Q3: How should FG-BA3301 Animal Tissue Universal Preservation Solution be stored? What is its shelf life?\u003c\/h4\u003e\n\u003cp\u003e\u003cspan\u003eA: It should be stored at -20°C in the dark, with a shelf life of one year. After receiving the product, it should be promptly dispensed into sterile containers of the required volume to avoid repeated freezing and thawing.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch4\u003e4. Q4: What precautions should be taken regarding aseptic operation when using this preservation solution?\u003c\/h4\u003e\n\u003cp\u003e\u003cspan\u003eA: This product is a sterile product. When using it, you should wear a lab coat and disposable gloves, pay attention to aseptic operation, and avoid microbial contamination to prevent affecting the tissue preservation effect and subsequent experimental results.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch4\u003e5. Q5: Is special treatment required for the preservation solution before use? What is the specific operation?\u003c\/h4\u003e\n\u003cp\u003e\u003cspan\u003eA: Before use, it is necessary to ensure that the animal tissue preservation solution is completely thawed and mixed uniformly. Moreover, before use, it must be fully inverted and shaken to make the components of the preservation solution completely uniform, ensuring that the concentration and components are consistent in every part. If there is a situation where it cannot be thawed, please contact the manufacturer.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch4\u003e6. Q6: What are the recommendations for cryopreservation tubes and tissue dosage during tissue sampling and dispensing?\u003c\/h4\u003e\n\u003cp\u003e\u003cspan\u003eA: It is recommended to use 2mL sterile and enzyme-free cryopreservation tubes for dispensing. The amount of fresh tissue stored in each cryopreservation tube is approximately 200mg (about the size of a soybean), and the volume used is 2mL.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch4\u003e7. Q7: Why is it necessary to clean the tissue after obtaining the tissue sample? What is the specific cleaning procedure?\u003c\/h4\u003e\n\u003cp\u003e\u003cspan\u003eA: Cleaning the tissue is to remove blood, mucus and other types of contaminants on the tissue surface, ensuring the tissue is clean. The specific steps are as follows: quickly place the tissue into an appropriate amount of PBS buffer, gently clamp the tissue with tweezers and stir slowly, gently rinse the tissue surface, and repeat the rinsing until the tissue surface is completely clean with no obvious visible impurities remaining. Also, remove necrotic tissues and pustular tissues.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch4\u003e8. Q8: What is the correct operation process for preserving the cleaned tissue with the preservation solution?\u003c\/h4\u003e\n\u003cp\u003e\u003cspan\u003eA: Carefully transfer the cleaned tissue into a 2mL cryopreservation tube, quickly fill the cryopreservation tube with the well-mixed animal tissue universal preservation solution to completely immerse the tissue; then tightly wrap the mouth of the cryopreservation tube with parafilm to ensure a tight seal without leakage, and finally place the cryopreservation tube in a low-temperature environment of 2°C~8°C for preservation.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch4\u003e9. Q9: After preserving the tissue with this preservation solution, within how long should subsequent experiments be carried out? How to handle the sample if transportation is needed?\u003c\/h4\u003e\n\u003cp\u003e\u003cspan\u003eA: After preserving the tissue, subsequent experiments such as cell suspension preparation must be carried out within 48 hours. If transportation is required, the sealed cryopreservation tube can be placed in a suitable foam box, and biological ice packs can be added according to the weather conditions. The principle is to keep the sample in an environment of 2°C~8°C without being frozen, and subsequent experiments should also be carried out within 48 hours.\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"5 mL\/Kit","offer_id":47833223987412,"sku":"FG-BA3301-5","price":19.0,"currency_code":"USD","in_stock":false},{"title":"100 mL\/Kit","offer_id":46299525054676,"sku":"FG-BA3301-100","price":159.0,"currency_code":"USD","in_stock":true},{"title":"500 ml\/kit","offer_id":47682218918100,"sku":"FG-BA3301-500","price":559.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/files\/BA3301_7480e09a-bfe4-4231-8fcc-c82cd9b4becf.png?v=1773992488"},{"product_id":"universal-cell-freezing-kit-fg-ba3309","title":"FireGene Universal Cell Freezing Kit - Long-Term Cryopreservation","description":"\u003ch3 id=\"overview\"\u003eOverview\u003c\/h3\u003e\n\u003cp\u003eThe\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eFireGene Universal Cell Freezing Kit\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eis a high-performance cryopreservation solution engineered to maintain long-term cell viability and functionality. Ideal for preserving primary cells, cell lines, and single-cell suspensions, this kit ensures high integrity of biological samples during ultra-low temperature storage.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch3 id=\"background-information\"\u003eBackground Information\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eDesigned for a wide range of\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eresearch and clinical applications\u003c\/strong\u003e:\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eCell line maintenance\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003ein basic research.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCell preservation\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003ein drug discovery and high-throughput screening.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePatient-derived cell storage\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003ein clinical trials.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBanking therapeutic cells\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003efor future use in cell therapy.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003eEnsures\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003epost-thaw recovery and functionality\u003c\/strong\u003e, which is critical for:\n\u003cul\u003e\n\u003cli\u003eCell-based assays.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSingle-cell analysis\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eand multi-omics workflows.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eOrganoid culture\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eand regenerative studies.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch3 id=\"detection-principle\"\u003eDetection Principle\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eUtilizes a\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003emulti-component cryopreservation system\u003c\/strong\u003e:\n\u003cul\u003e\n\u003cli\u003eContains\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003epenetrating cryoprotectants\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003ethat reduce intracellular ice formation.\u003c\/li\u003e\n\u003cli\u003eMinimizes\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eosmotic stress\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eand stabilizes membranes.\u003c\/li\u003e\n\u003cli\u003eCompatible with\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003econtrolled cooling and thawing protocols\u003c\/strong\u003e.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003eBenefits:\n\u003cul\u003e\n\u003cli\u003ePreserves\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ecell structure, membrane integrity, and viability\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eExtends usability of cell samples across long-term storage.\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eSpecifications\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eSingle-cell sequencing, cell culture or other cell-related detections\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eCompatible Sample Types\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eSingle-cell suspension; primary cells; cell lines\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eSupported Instruments\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eCell Freezing Container\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e-20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eShelf-life\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eKit Components\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e100ml\/kit\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 60%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e100 mL\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003eUCF\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e500ml\/kit\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 60%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e500 mL\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003eUCF\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eProduct FAQ\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e1.    Q: The kit is labeled \"universal\"—is it suitable for all mammalian cells? What is its cryopreservation effect on sensitive cells (such as nerve cells and stem cells)? Can it be used for insect cells or plant cells?\u003c\/strong\u003e\u003c\/span\u003e\u003cspan\u003eA: The kit is suitable for most mammalian primary cells (e.g., hepatocytes, cardiomyocytes) and immortalized cell lines (e.g., HeLa, CHO cells). The cryopreservation solution contains low-toxicity protective components that reduce ice crystal damage, and the survival rate of sensitive cells after resuscitation is usually over 70% (≥85% for ordinary cells). However, it is not suitable for insect cells or plant cells. The cell membrane structure and antifreeze mechanism of these cells are significantly different from those of mammals, so dedicated cryopreservation solutions (e.g., insect cell cryopreservation solution, plant protoplast cryopreservation solution) must be used. Direct use of this kit may lead to resuscitation failure.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e2.    Q: The instruction manual recommends a cryopreservation density of 1×10⁶ cells\/mL. Will insufficient cell quantity (e.g., only 5×10⁵ cells) or excessive quantity (e.g., 3×10⁶ cells) affect the cryopreservation effect? How to adjust?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Yes, it will affect the effect. Insufficient density (\u0026lt;5×10⁵ cells\/mL) causes cells to be \"isolated\" during cryopreservation, lacking the synergistic protection of adjacent cells. This increases the risk of ice crystal damage and reduces the survival rate after resuscitation. Excessive density (\u0026gt;2×10⁶ cells\/mL) leads to cell aggregation, preventing the cryopreservation solution from fully wrapping each cell; additionally, cells tend to clump during resuscitation, affecting dispersibility. Adjustment methods: For insufficient quantity, concentrate cells by centrifugation (follow the centrifugation conditions for the corresponding cell type, e.g., 300×g for 5 minutes for human PBMCs), discard part of the supernatant to increase density. For excessive quantity, dilute with cryopreservation solution to 1×10⁶-2×10⁶ cells\/mL, and aliquot into multiple cryovials to avoid exceeding the density limit in a single vial.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e3.    Q: The cryopreservation solution should be stored at -20°C in the dark. If unused after thawing, how to handle the remaining solution? Can it be frozen again? What are the impacts of repeated freezing and thawing?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Unused thawed cryopreservation solution should be stored at 4°C in the dark within 24 hours and cannot be frozen again. Repeated freezing and thawing damages the protective components in the solution (e.g., decreased DMSO stability, decomposition of nutrients), drastically reducing its antifreeze ability. Each freeze-thaw cycle may lower the cell resuscitation survival rate by 15%-20%; after more than 3 cycles, the solution becomes basically ineffective and cannot protect cells. It is recommended to aliquot the cryopreservation solution into 1mL\/tube or 5mL\/tube (using sterile cryovials) based on single-use volume upon receiving the kit, seal tightly, store at -20°C, and take one tube per experiment to avoid repeated freezing and thawing.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e4.    Q: Step 6 mentions \"using a programmed freezing box to ensure a cooling rate of approximately 1°C\/min\". If a programmed freezing box is not available in the laboratory, can other methods be used as substitutes? What problems will occur if directly placing the cryovial into a -80°C refrigerator?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Without a programmed freezing box, a homemade alternative can be used: place the cryovial into a foam box (thickness ≥5cm) filled with isopropyl alcohol, then put it into a -80°C refrigerator. Isopropyl alcohol buffers the cooling rate to approximately 1°C\/min (error ±0.5°C), but the foam box must be sealed to prevent isopropyl alcohol evaporation. Do not place directly into a -80°C refrigerator—the direct cooling rate can reach 5-10°C\/min, causing rapid formation of large ice crystals inside cells, which puncture the cell membrane. The survival rate after resuscitation may be lower than 30%, or even zero.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e5.    Q: Cells should be transferred to liquid nitrogen for storage 24 hours after cryopreservation. If transfer is delayed beyond 24 hours (e.g., forgotten and left in a -80°C refrigerator for 48 hours), can the cells still be normally resuscitated and used?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Cells left in a -80°C refrigerator for 48 hours can still be attempted for resuscitation, but two points should be noted: First, cell viability must be tested after resuscitation (using trypan blue staining; viability ≥60% is required for use). A -80°C refrigerator is for \"semi-long-term storage\"; beyond 24 hours, some cells gradually lose viability due to slow ice crystal damage, with a potential viability decrease of 10%-25%. Second, for low-temperature-sensitive cells (e.g., stem cells), viability may drop below 50% after 48 hours, making them unsuitable for critical subsequent experiments (e.g., cell culture, drug screening) and only usable for preliminary experiments. For long-term storage, cells must be transferred to liquid nitrogen (-196°C) within 24 hours—liquid nitrogen almost stops cell metabolism, allowing cells to maintain high viability for several years.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e6.    Q: Step 2 of cell resuscitation requires \"shaking the cryovial quickly to thaw the cryopreservation solution rapidly\". At what exact thawing stage should it be stopped? What impacts will incomplete thawing or overheating (e.g., water bath temperature exceeding 37°C) cause?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Stop thawing when \"only a needle-tip-sized ice crystal remains\" (usually 30-60 seconds). At this stage, the cryopreservation solution is translucent with no obvious solid particles; immediately remove the vial. The remaining small ice crystal will melt naturally when adding medium in subsequent steps, avoiding cell damage from excessive shaking. Incomplete thawing (with obvious ice crystals) causes osmotic shock when adding medium—sudden melting of ice crystals disrupts the cell membrane. Water bath temperature exceeding 37°C (e.g., 40°C) induces heat stress in cells, leading to abnormal enzyme activity and a large number of dead cells after resuscitation. The water bath temperature must be strictly controlled at 37°C±1°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e7.    Q: Step 4 mentions \"adding the cryopreservation solution drop by drop into the pre-warmed medium while shaking\". What are the requirements for dropping speed and shaking intensity? What consequences will occur if pouring all at once quickly?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Control the dropping speed to \"1 drop every 2-3 seconds\", and shake with intensity such that \"the liquid in the centrifuge tube rotates slightly without violent vortices\". The purpose is to slowly mix the cryopreservation solution (containing DMSO) with the medium, gradually reducing the DMSO concentration to avoid severe osmotic changes caused by sudden concentration drops. Do not pour all at once quickly—this causes the local DMSO concentration to drop from 10%-20% to 2%-4% instantly. Cells undergo dehydration or swelling due to sudden osmotic changes, leading to cell membrane rupture. The proportion of dead cells can increase by more than 30%, severely affecting the resuscitation effect.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e8.    Q: Centrifugation conditions vary by cell type. If the centrifugation parameters for the target cell (e.g., primary cardiomyocytes) are unknown, how to set the conditions? Must the centrifugation temperature be 4°C?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: For unknown parameters, refer to the \"universal centrifugation conditions\": 300×g at 4°C for 5 minutes. This condition is suitable for over 90% of mammalian cells—it effectively pellets cells without crushing them (e.g., fragile cardiomyocytes are prone to rupture if centrifugal force exceeds 500×g). If cells are not sufficiently pelleted after centrifugation (e.g., suspension cells), slightly increase the centrifugal force to 400×g while keeping the time unchanged. The centrifugation temperature is recommended to be 4°C, as 4°C reduces cell metabolism and minimizes energy consumption during centrifugation. Room-temperature centrifugation (around 25°C) has little impact on ordinary immortalized cells but may reduce the viability of sensitive cells (e.g., nerve cells) by 5%-10%, so 4°C is preferred.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e9.    Q: After resuscitation, the supernatant is discarded and cells are resuspended in medium. Are there any requirements for medium selection? Must RPMI 1640 medium be used? Can DMEM or serum be used as a substitute?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Medium selection must match the cell type and is not limited to RPMI 1640: Adherent cells (e.g., HeLa) commonly use DMEM medium, suspension cells (e.g., Jurkat) commonly use RPMI 1640, and primary cells require dedicated medium (e.g., DMEM\/F12 for cardiomyocytes). The core requirement is that the medium meets the cell's growth needs. Serum cannot be used as a substitute—serum lacks basic nutrients required for cell growth (e.g., amino acids, vitamins) and only provides partial factors. Resuspending cells in serum will prevent normal cell proliferation and even cause gradual cell death.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e10.    Q: If the cryovial leaks during storage in liquid nitrogen (e.g., loose seal at the cap), and the cryopreservation solution is turbid with white flocculent precipitates during resuscitation, can the cells still be used? How to prevent leakage?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Turbid cryopreservation solution with white flocculent precipitates indicates liquid nitrogen has infiltrated the vial—cells are either contaminated or dead due to sudden osmotic changes, so they cannot be used and must be discarded directly to avoid laboratory contamination. Key measures to prevent leakage: First, select \"liquid nitrogen-compatible\" cryovials (marked \"LN2 Compatible\" on the wall) and never use ordinary centrifuge tubes. Second, when tightening the cap, follow the steps: \"tighten gently → loosen half a turn → retighten\" to avoid over-tightening or under-tightening caused by temperature changes. Third, when transferring to a liquid nitrogen tank, first pre-cool the vial in the liquid nitrogen gas phase zone (\u0026lt;-150°C) for 10 minutes, then move it to the liquid phase zone to reduce stress-induced leakage at the cap due to temperature differences.\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"100 ml\/kit","offer_id":46299525087444,"sku":"FG-BA3309-100","price":139.0,"currency_code":"USD","in_stock":true},{"title":"500 ml\/kit","offer_id":47682339438804,"sku":"FG-BA3309-500","price":259.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/files\/BA3309_439443e2-5c1c-415d-a1b6-c4599716108e.png?v=1773997648"},{"product_id":"animal-tissue-freezing-kit-fg-ba3312","title":"FireGene Animal Tissue Freezing Kit - Gradient-Freezing Protection","description":"\u003ch3 id=\"overview\"\u003eOverview\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Animal Tissue Freezing Kit\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eis engineered to preserve animal tissue samples through a gradient freezing process enhanced with cryoprotectants. Ideal for applications such as single-cell sequencing, tissue culture, and molecular biology, this kit ensures tissue integrity and viability post-thaw for high-quality experimental outcomes.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch3 id=\"background-information\"\u003eBackground Information\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eDesigned to support:\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eSingle-cell studies\u003c\/strong\u003e, where tissue architecture must be preserved.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence and biomarker analysis\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003ein disease models.\u003c\/li\u003e\n\u003cli\u003eLong-term\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ebiorepository storage\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eof experimental tissues.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003eApplicable to a wide range of tissues, including\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eskin, liver, muscle, kidney, brain, and fat\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eSuitable for research into disease heterogeneity, drug efficacy, and\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eregenerative medicine\u003c\/strong\u003e.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch3 id=\"detection-principle\"\u003eDetection Principle\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eUses\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003egradient freezing\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eand a proprietary cryoprotectant system:\n\u003cul\u003e\n\u003cli\u003eSamples are\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003efrozen in controlled stages\u003c\/strong\u003e, reducing osmotic stress.\u003c\/li\u003e\n\u003cli\u003ePrevents formation of damaging\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eice crystals\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eUpon use, tissues are\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003erapidly thawed and cultured\u003c\/strong\u003e, restoring activity and cellular function.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003eEnd result:\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eReliable recovery\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eof viable tissue samples ready for histology, cell culture, and genomic analysis.\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eSpecifications\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eSingle-cell sequencing, cell culture or other cell-related detections\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eCompatible Sample Types\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eAnimal tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eSupported Instruments\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eCell Freezing Container\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e-20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eShelf-life\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eKit Components\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e100ml\/kit\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 60%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e100 mL\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003eATF\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e500ml\/kit\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 60%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e500 mL\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003eATF\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProduct  FAQ\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e1.    Q: Is this cryopreservation solution suitable for all animal tissues? What is its cryopreservation effect on sensitive tissues (e.g., neural tissue, embryonic tissue)? Can it be used for plant tissues or microbial samples?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The cryopreservation solution is suitable for most mammalian tissues (e.g., liver, kidney, heart, skin) and also has good adaptability to sensitive tissues (neural tissue, embryonic tissue). It contains low-toxicity protective components that reduce ice crystal damage to fragile cells. After resuscitation, the cell viability of sensitive tissues can usually reach over 65% (viability of ordinary tissues ≥80%). However, it is not suitable for plant tissues or microbial samples. The cell wall structure of plant tissues and the metabolic mechanism of microbes are significantly different from those of animal tissues, and the components of this cryopreservation solution cannot meet their protection needs, which may lead to resuscitation failure. When used for sensitive tissues, it is recommended to cut the tissue into smaller pieces (0.5mm×0.5mm×0.5mm) to ensure full infiltration of the cryopreservation solution and enhance the protection effect.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e2.    Q: The instruction manual requires approximately 200mg of tissue (size of a soybean) for cryopreservation. Will insufficient tissue quantity (e.g., 50mg) or excessive quantity (e.g., 300mg) affect the cryopreservation effect? How to adjust the operation?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Yes, it will affect the effect. When the tissue quantity is insufficient (\u0026lt;100mg), the cryopreservation solution is relatively excessive, and the tissue is prone to overexposure to protective components, which may cause cell metabolic disorders after resuscitation. When the tissue quantity is excessive (\u0026gt;250mg), the cryopreservation solution cannot fully wrap the cells inside the tissue, leading to necrosis of local cells due to lack of protection and decreased viability after resuscitation. Adjustment methods: ① For insufficient quantity, multiple small-dose tissue samples (50-100mg each) can be combined in the same cryovial, and the dosage of cryopreservation solution can be calculated based on the total weight (10 times the volume of the total weight) to avoid too little single-dose tissue. ② For excessive quantity, divide the tissue into multiple cryovials for cryopreservation, with each vial containing 150-200mg of tissue, to ensure that each tissue sample is fully covered by the cryopreservation solution and meets the sample quantity requirements of subsequent experiments.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e3.    Q: Step 5 requires \"the volume of cryopreservation solution is 10 times the weight of the tissue\". If the tissue contains a lot of water (e.g., edematous tissue) or fat (e.g., adipose tissue), does this ratio need to be adjusted? Will the adjustment affect the cryopreservation effect?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The ratio needs to be flexibly adjusted according to the tissue characteristics: ① For edematous tissue (high water content): The volume of cryopreservation solution can be reduced to 8 times the weight of the tissue to prevent the protective components from being diluted after water mixes with the cryopreservation solution, which would weaken the protection effect. ② For adipose tissue (low density, easy to float): The volume of cryopreservation solution needs to be increased to 12 times the weight of the tissue to ensure that the adipose tissue is completely immersed in the cryopreservation solution and avoid local cell necrosis caused by exposure to air. After adjusting the ratio, the tissue type and adjusted dosage should be marked on the cryovial. During subsequent resuscitation, the conventional steps can be followed. As long as the tissue is fully wrapped by the cryopreservation solution, the final cryopreservation effect will not be significantly affected.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e4.    Q: Step 7 requires \"using a programmed freezing box to ensure a cooling rate of approximately 1℃\/min\". If there is no programmed freezing box in the laboratory, can other methods be used as substitutes? What problems will occur if the tissue is directly placed in a -80℃ refrigerator or liquid nitrogen?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Without a programmed freezing box, a homemade alternative device can be used: place the cryovial in a foam box (thickness ≥5cm) filled with isopropyl alcohol, seal the box, and then put it into a -80℃ refrigerator. Isopropyl alcohol can buffer the cooling rate to be close to 1℃\/min (error ±0.5℃), but it is necessary to ensure that the foam box is not damaged to prevent isopropyl alcohol from volatilizing. Do not directly place the tissue in a -80℃ refrigerator or liquid nitrogen: Direct placement in a -80℃ refrigerator results in a cooling rate of 5-8℃\/min, which easily forms large ice crystals that pierce cells. Direct placement in liquid nitrogen causes an abrupt cooling rate of over 100℃\/min, and the tissue will suffer from \"vitrification damage\" due to the instantaneous low temperature, resulting in almost no viable cells after resuscitation. A programmed cooling transition is essential.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e5.    Q: The cryopreservation solution needs to be stored at -20℃ in the dark. If it is not used up after thawing, how to handle the remaining cryopreservation solution? Can it be frozen again? What are the effects of repeated freezing and thawing?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The remaining cryopreservation solution after thawing should be stored at 4℃ in the dark within 24 hours and cannot be frozen again. Repeated freezing and thawing will damage the protective components in the cryopreservation solution (e.g., decreased stability of DMSO, denaturation of protein protectants), leading to a sharp reduction in antifreeze ability. Each freeze-thaw cycle may reduce the tissue resuscitation viability by 15%-20%; after more than 3 freeze-thaw cycles, the cryopreservation solution basically becomes ineffective and cannot protect tissue cells. It is recommended that after receiving the kit, divide the 100mL cryopreservation solution into 10mL\/tube or 5mL\/tube (using sterile cryovials) according to the single-use cryopreservation demand, seal them, store at -20℃, and take one tube for each experiment to avoid repeated freezing and thawing.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e6.    Q: Step 2 requires \"quickly shaking and thawing in a 37℃ water bath\" during resuscitation. What are the requirements for the frequency and intensity of \"quick shaking\"? What consequences will occur if thawing is incomplete or the water temperature deviates from 37℃ (e.g., 35℃, 40℃)?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Requirements for \"quick shaking\": Hold the cryovial and shake it gently in a horizontal direction at a frequency of about 1-2 times per second. The intensity should be such that the liquid in the tube rotates slightly without generating violent vortices. The purpose is to allow the cryopreservation solution to be evenly heated and accelerate thawing. If thawing is incomplete (with obvious ice crystals remaining), the ice crystals will melt suddenly during the subsequent addition of medium, causing osmotic shock and damaging tissue cells. When the water temperature is 35℃, the thawing speed is too slow, and the tissue is exposed to a low-temperature environment for a longer time, resulting in an additional 10%-15% decrease in viability. When the water temperature is 40℃, the high temperature will cause heat stress to tissue cells, leading to abnormal enzyme activity and massive cell death after resuscitation. The temperature of the water bath must be strictly controlled at 37℃±1℃.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e7.    Q: Step 4 requires \"adding the cryopreservation solution drop by drop to the pre-warmed medium while shaking\". How to control the dropping speed and shaking intensity? What consequences will occur if it is poured all at once quickly?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The dropping speed should be controlled at \"1 drop every 2-3 seconds\", and the shaking intensity should be such that \"the liquid in the centrifuge tube rotates slowly without obvious stratification\". The core is to allow the cryopreservation solution (containing DMSO) to mix slowly with the medium, gradually reducing the DMSO concentration to avoid severe osmotic fluctuations of cells caused by sudden concentration changes. Do not pour it all at once quickly. Pouring all at once will cause the local DMSO concentration to drop from about 10% to less than 2% instantly. Tissue cells will experience dehydration or swelling due to sudden osmotic changes, leading to cell membrane rupture. The viability after resuscitation may decrease by more than 40%, and in severe cases, the tissue cannot be used for subsequent experiments.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e8.    Q: The centrifugation parameters require \"centrifugation at 4℃, 300×g for 5 minutes\". What effects will insufficient centrifugation time (e.g., 3 minutes) or excessive rotation speed (e.g., 500×g) have on the resuscitated tissue? Can room-temperature centrifugation replace 4℃ centrifugation?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: If the centrifugation time is insufficient (\u0026lt;5 minutes), tissue cells cannot be fully precipitated and will be discarded with the supernatant, resulting in sample loss and reduced yield. If the rotation speed is excessive (\u0026gt;400×g), the excessive centrifugal force will squeeze tissue cells, especially fragile tissues (e.g., embryonic tissue), which may cause cell rupture and a 25%-30% decrease in viability. Room-temperature centrifugation cannot replace 4℃ centrifugation. Room temperature will accelerate the metabolism of tissue cells, and the residual cryopreservation solution has enhanced activity at room temperature, which may further damage cells. Centrifugation at 4℃ can maintain a low metabolic state of cells and reduce viability loss during centrifugation.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e9.    Q: Step 5 requires \"adding an appropriate tissue dissociation solution to digest into a single-cell suspension\" after resuscitation. What specifically does \"appropriate tissue dissociation solution\" refer to? Can other dissociation kits of this brand (e.g., BA3303, BA3305) be used? What should be noted during digestion?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: \"Appropriate tissue dissociation solution\" refers to a dedicated dissociation kit matching the type of cryopreserved tissue. For example, BA3323 Liver Tissue Dissociation Kit is used for cryopreserved liver tissue, BA3305 Mouse Brain Tissue Dissociation Kit for cryopreserved brain tissue, and BA3320 Tumor Tissue Dissociation Kit for cryopreserved tumor tissue. Dissociation kits of this brand corresponding to the tissue type can be used, as their enzymatic hydrolysis parameters have better adaptability to the tissue state after resuscitation with the cryopreservation solution. Notes during digestion: ① Resuscitated tissue is relatively fragile, so the digestion time should be 20%-30% shorter than that of fresh tissue. ② Conduct quality inspection every 10 minutes to avoid excessive digestion leading to decreased cell viability and ensure a balance between single-cell yield and viability.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e10.    Q: If the cryovial is stored in a -80℃ refrigerator for more than 6 months, or if the temperature fluctuates during transportation (e.g., from -80℃ to -20℃ and then back), can the resuscitated tissue still be used for single-cell sequencing experiments?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Storage in a -80℃ refrigerator is not recommended for more than 6 months. After 6 months, the viability of tissue cells will gradually decrease over time (5%-8% decrease per month), and the viability may be lower than 50% after 6 months. Forcing its use in single-cell sequencing will lead to reduced data quality (e.g., low cell capture rate, gene expression deviation). Temperature fluctuations during transportation (-80℃ to -20℃) will cause the tissue to undergo repeated \"micro-freezing and thawing\", damaging the cell structure. Even if the temperature returns to normal, the viability after resuscitation will decrease by more than 30%, so it is not recommended for high-precision experiments such as single-cell sequencing, and can only be used for preliminary experiments or morphological observation. For long-term storage, it is recommended to transfer the tissue to liquid nitrogen (-196℃) within 24 hours of placement in a -80℃ refrigerator, where it can be stored for more than 1 year with stable viability.\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"5 ml\/kit","offer_id":47833390678228,"sku":"FG-BA3312-5","price":39.0,"currency_code":"USD","in_stock":true},{"title":"100 ml\/kit","offer_id":46299527413972,"sku":"FG-BA3312-100","price":429.0,"currency_code":"USD","in_stock":true},{"title":"500 ml\/kit","offer_id":47682236022996,"sku":"FG-BA3312-500","price":1499.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/files\/BA3312.png?v=1773993779"},{"product_id":"pancreas-tissue-storage-kit-fg-ba3313","title":"FireGene Pancreas Tissue Storage Kit - For Reliable Cell Recovery","description":"\u003ch3 id=\"overview\"\u003eOverview\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Pancreas Tissue Storage Kit\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eis engineered to preserve pancreatic tissue integrity for downstream analysis in both research and clinical applications. This advanced preservation solution ensures tissue viability by maintaining physiological balance and mitigating cellular stress, making it ideal for single-cell sequencing, biomarker analysis, and long-term storage.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch3 id=\"background-information\"\u003eBackground Information\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eDesigned to address the need for\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ehigh-quality pancreatic tissue samples\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003ein:\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eSingle-cell sequencing\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eand transcriptomic profiling.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiomarker analysis\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eusing immunofluorescence and histology.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLong-term biobanking\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003efor pancreatic disease research and pathology.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003eSuitable for use in\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eexperimental models of diabetes, pancreatitis, and pancreatic cancer\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003ePreserves cellular structure and function in\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003efresh or frozen tissues\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eto support reproducibility and downstream experimental success.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch3 id=\"detection-principle\"\u003eDetection Principle\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eThe kit employs a\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003especialized buffer system\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003ethat:\n\u003cul\u003e\n\u003cli\u003eMaintains\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eosmotic and metabolic equilibrium\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eto prevent tissue damage.\u003c\/li\u003e\n\u003cli\u003eIncludes\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eantioxidants\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003ethat reduce oxidative stress and neutralize reactive oxygen species.\u003c\/li\u003e\n\u003cli\u003eInhibits\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eapoptotic and necrotic cell death pathways\u003c\/strong\u003e, ensuring preserved tissue integrity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003eAs a result:\n\u003cul\u003e\n\u003cli\u003eSamples remain viable and structurally intact, supporting accurate molecular and cellular analyses after thawing.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eSpecifications\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eSingle-cell sequencing, cell culture or other cell-related detections\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eCompatible Sample Types\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003ePancreas tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eSupported Instruments\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e\/\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e-80 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eShelf-life\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e1 month\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eKit Components\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 60%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003e8 Reactions\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003ePancreas Tissue Storage Solution\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e8 × 1.8 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eProduct FAQ\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e1.    Q: Is this preservation solution only suitable for mammalian pancreatic tissue? Are there differences in preservation effects on different parts of the pancreas (e.g., islets, exocrine portion)? Can it be used for pancreatic tumor tissue or non-mammalian pancreatic tissue (e.g., avian pancreas)?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The preservation solution is only suitable for normal mammalian pancreatic tissue and is not temporarily applicable to pancreatic tumor tissue or non-mammalian pancreatic tissue. Pancreatic tumor tissue has abnormal cell structure and metabolic characteristics, and the preservation solution cannot maintain its physiological activity; the cell membrane structure and anti-damage mechanism of non-mammalian pancreatic tissue (such as avian pancreas) are significantly different from those of mammals, leading to massive cell death after preservation. There are slight differences in the preservation effects on different parts of the pancreas: islet tissue (containing sensitive endocrine cells) has stricter requirements for preservation conditions, and it is recommended to prioritize processing within the 48-hour preservation period; the exocrine portion has slightly stronger tolerance but still needs to complete experiments within the specified time. Both can maintain sensitivity to glucose stimulation through the preservation solution without significant differences in activity.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e2.    Q: The instruction manual recommends storing 200mg of pancreatic tissue per cryovial. Will insufficient tissue quantity (e.g., 50mg) or excessive quantity (e.g., 300mg) affect the preservation effect? How to adjust the operation?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Yes, it will affect the effect. ① Insufficient tissue quantity (\u0026lt;100mg): The preservation solution is relatively excessive, which will not directly damage cells but will increase the difficulty of tissue transfer and cleaning in subsequent experiments, and easily cause the tissue to float due to excessive liquid, affecting subsequent dissociation efficiency. It is recommended to combine multiple small-dose tissues (50-100mg each) into the same cryovial, control the total weight at 150-200mg, and fill the cryovial with the preservation solution according to the conventional dosage. ② Excessive tissue quantity (\u0026gt;250mg): The cryovial (2mL specification) cannot hold a sufficient amount of preservation solution, causing part of the tissue to be exposed to air and undergo dry necrosis. The tissue needs to be stored in multiple cryovials, with the tissue quantity in each vial strictly controlled at 180-220mg to ensure the tissue is completely immersed in the preservation solution.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e3.    Q: The preservation solution needs to be stored at -80℃ in the dark with a validity period of 1 month. Can it still be used if it exceeds the validity period but has no obvious changes in appearance (e.g., no turbidity or precipitation)? Will temporary thawing (e.g., placing at room temperature for 10 minutes when taking) during storage at -80℃ affect the effect?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The preservation solution that exceeds the validity period cannot be used anymore. Even if there is no change in appearance, its internal protective components (such as anti-self-digestion factors and activity stabilizers) will degrade over time, unable to prevent the self-digestion of pancreatic tissue, which may lead to massive damage to islet cells. Temporary thawing during storage at -80℃ (10 minutes at room temperature) needs to be judged according to the situation: ① Incomplete thawing (only a small amount of thawing on the tube wall): Immediately put it back at -80℃, and it can be used normally later. ② Complete thawing: It needs to be stored at 4℃ in the dark within 24 hours and used as soon as possible, and cannot be frozen again. The activity of protective components will decrease by 10%-15% after complete thawing, but it can still meet the short-term preservation needs. If it is not used within 24 hours, it should be discarded.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e4.    Q: Step 3 requires \"gently rinsing the tissue with PBS buffer until the surface is clean\". What is the operational standard for \"gentle\"? What consequences will occur if the rinsing force is too strong or blood and mucus are not cleaned?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The operational standard for \"gentle\" is: gently clamp the edge of the tissue with tweezers (avoid clamping the main part of the tissue), stir slowly in PBS at a frequency of once per second, with each rinsing time not exceeding 30 seconds, and repeat 3-4 times until there is no visible blood or mucus residue in the PBS. Excessive rinsing force will cause damage to pancreatic tissue, release internal digestive enzymes, and accelerate tissue self-digestion; uncleaned blood will introduce red blood cell impurities, which are easily confused with pancreatic cells during subsequent dissociation, affecting the accuracy of cell counting; mucus will wrap the tissue surface, preventing the penetration of the preservation solution, leading to necrosis of local cells due to lack of protection.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e5.    Q: Step 4 requires \"filling the cryovial with the preservation solution to completely immerse the tissue\". What impact will incomplete filling (e.g., filling only 2\/3 of the cryovial) have on the preservation effect? Can PBS be used to supplement to a full cryovial?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Incomplete filling of the preservation solution will cause the tissue to contact with air, leading to oxidative damage. At the same time, the concentration of the preservation solution may change due to the volatilization of air above the liquid surface, unable to maintain a stable preservation environment. The cell activity may decrease by 25%-30% after 24 hours of preservation, and basically lose experimental value after 48 hours. PBS cannot be used to supplement to a full cryovial. PBS has no components for anti-self-digestion and maintaining glucose stimulation sensitivity, which will dilute the concentration of effective components in the preservation solution, destroy the protection system, lead to intensified self-digestion of pancreatic tissue, and a sharp decline in the activity of islet cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e6.    Q: The preservation temperature requires 2℃-8℃. How to stabilize the temperature if the temperature fluctuation range of the laboratory refrigerator is large (e.g., 1℃-9℃)? What problems will occur if the temperature is lower than 2℃ (e.g., 0℃) or higher than 8℃ (e.g., 10℃)?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: When the refrigerator temperature fluctuates greatly, the cryovial can be placed in a constant-temperature incubator (adjusted to 5℃), or wrapped with thermal insulation cotton and placed in the middle layer of the refrigerator (an area with relatively stable temperature), avoiding positions with severe temperature fluctuations such as the refrigerator door and evaporator. A temperature lower than 2℃ (e.g., 0℃) will cause local freezing of the preservation solution, and ice crystals will pierce the pancreatic cell membrane. In particular, islet cells are more sensitive to low temperatures, and their activity will decrease by more than 40%; a temperature higher than 8℃ (e.g., 10℃) will accelerate the degradation of protective components in the preservation solution and promote the activity of self-digestive enzymes in pancreatic tissue. The tissue may become significantly turbid after 12 hours of preservation and cannot be used for subsequent experiments.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e7.    Q: Step 5 requires \"the number of biological ice packs depends on the weather conditions to ensure the sample is at 2℃-8℃ without freezing\". How to adjust the number of ice packs according to the weather? What impact will transportation time exceeding 24 hours have?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Standards for adjusting the number of ice packs: ① Normal temperature weather (15℃-25℃): Place 2 500g biological ice packs (1 on top and 1 on bottom) in a 500mL foam box. ② High-temperature weather (\u0026gt;25℃): Increase to 4 500g biological ice packs (1 on each side, with the sample placed in the middle), and attach thermal insulation film inside the foam box. ③ Low-temperature weather (\u0026lt;15℃): Place 1 500g biological ice pack, and fill the space around the sample with paper towels to avoid freezing due to too low temperature. Transportation time exceeding 24 hours will significantly increase risks: even if the temperature is stable, the sensitivity of pancreatic tissue to glucose stimulation will gradually decrease after more than 24 hours in the preservation solution. The longer the transportation time within the 48-hour preservation period, the greater the deviation of subsequent experimental data. It is recommended to control the transportation time within 12 hours and check the tissue status immediately after arrival.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e8.    Q: The preservation solution needs to be \"completely thawed and mixed\" before use. What is the specific thawing method? What consequences will occur if a 37℃ water bath is used to accelerate thawing or if it is used without complete thawing?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The thawing method is natural thawing at room temperature (18℃-25℃). Take the preservation solution out of -80℃, place it in a room-temperature environment, invert and mix it once every 10 minutes until the liquid is completely clear without ice crystals. A 37℃ water bath cannot be used to accelerate thawing. A 37℃ water bath will cause the local temperature of the preservation solution to be too high, destroying the activity of anti-self-digestion factors and making the preservation solution invalid; using it without complete thawing, the remaining ice crystals will melt slowly when in contact with the tissue, generating osmotic pressure shock and damaging pancreatic cells. In particular, it will lead to a significant decline in the activity of islet cells, affecting the subsequent detection of blood glucose regulation function.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e9.    Q: Cell suspension preparation experiments need to be carried out within 48 hours of preservation. Can the preservation time be extended to 72 hours if the operation cannot be carried out on time due to special circumstances? How to judge whether the tissue is still usable after extension?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Extending the preservation time to 72 hours is not recommended. After more than 48 hours, the protective effect of the preservation solution will decrease sharply, the self-digestion of pancreatic tissue will intensify, and the cell activity may be lower than 50%, which cannot meet the needs of high-precision experiments such as high-throughput single-cell sequencing. If the preservation time needs to be extended due to special circumstances, the experiment must be completed within 60 hours, and the following standards should be used to judge whether the tissue is still usable before use: ① Observe the tissue appearance: no obvious swelling, turbidity, or peculiar smell. ② Stain a small amount of tissue with trypan blue: cell viability ≥60%. ③ Detect glucose stimulation sensitivity: after treatment with low-glucose medium, the insulin secretion reaches more than 50% of that of fresh tissue. Only when the above three conditions are met can the tissue be used; otherwise, it should be discarded.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e10.    Q: Compared with other tissue preservation solutions of this brand (e.g., \u003ca href=\"https:\/\/firegene.com\/products\/animal-tissue-storage-kit-fg-ba3301?_pos=1\u0026amp;_sid=274217ed7\u0026amp;_ss=r\"\u003eFG-BA3301 Animal Tissue Universal Preservation Solution\u003c\/a\u003e), what are the core advantages of this pancreatic tissue-specific preservation solution? Can BA3301 be used as a substitute for this product to preserve pancreatic tissue?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The core advantages are reflected in two aspects: ① Aiming at the \"easy self-digestion\" characteristic of pancreatic tissue, anti-self-digestion factors are added to inhibit the activity of digestive enzymes such as trypsin in the pancreas and avoid tissue self-damage. ② It contains a special glucose stimulation-sensitive stabilizer, which can maintain the response ability of pancreatic tissue to changes in glucose concentration and retain its physiological activity of regulating blood glucose, which is not available in universal preservation solutions. \u003ca href=\"https:\/\/firegene.com\/products\/animal-tissue-storage-kit-fg-ba3301?_pos=1\u0026amp;_sid=274217ed7\u0026amp;_ss=r\"\u003eFG-BA3301\u003c\/a\u003e cannot be used as a substitute. \u003ca href=\"https:\/\/firegene.com\/products\/animal-tissue-storage-kit-fg-ba3301?_pos=1\u0026amp;_sid=274217ed7\u0026amp;_ss=r\"\u003eFG-BA3301\u003c\/a\u003e has no anti-self-digestion components. When preserving pancreatic tissue, obvious self-digestion will occur within 24 hours, a large number of islet cells will die, and the glucose stimulation sensitivity cannot be maintained, making it impossible to obtain accurate physiological function data in subsequent experiments.\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"8 Reactions\/kit","offer_id":46299527446740,"sku":"FG-BA3313-8rxns","price":359.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/files\/BA3313.png?v=1774421748"},{"product_id":"animal-gastrointestinal-tissue-storage-solution","title":"FireGene Animal Gastrointestinal Tissue Storage Solution for Single-Cell Sample Preservation","description":"\u003cp\u003e\u003cstrong\u003eFireGene Animal Gastrointestinal Tissue Storage Solution\u003c\/strong\u003e provides a simple and effective method for preserving ex vivo animal gastrointestinal tissues after collection. The solution uses low-temperature preservation to reduce cellular metabolic activity and help maintain cell viability during storage or transport. It is suitable for short-term preservation of fresh gastrointestinal tissue samples before downstream experiments such as cell suspension preparation and high-throughput single-cell sequencing. The protocol recommends storing approximately 200 mg of fresh tissue per 2 mL cryovial and processing samples within 48 hours.\u003c\/p\u003e\n\u003ch2\u003eBackground Information\u003c\/h2\u003e\n\u003cp data-start=\"1362\" data-end=\"1653\"\u003eAnimal gastrointestinal tissue can change quickly after collection due to cellular stress, enzymatic activity, mucus contamination, and transport conditions. Short-term preservation at low temperature helps slow cellular metabolism and maintain tissue condition before downstream processing.\u003c\/p\u003e\n\u003cp data-start=\"1655\" data-end=\"1913\"\u003e\u003cstrong data-start=\"1655\" data-end=\"1715\"\u003eFireGene Animal Gastrointestinal Tissue Storage Solution\u003c\/strong\u003e is designed for ex vivo animal gastrointestinal tissue samples that require temporary storage or transport before cell suspension preparation, single-cell sequencing, or related research workflows.\u003c\/p\u003e\n\u003ch3 data-section-id=\"15x6sgr\" data-start=\"1915\" data-end=\"1933\"\u003eResearch Areas\u003c\/h3\u003e\n\u003cp data-start=\"1935\" data-end=\"2081\"\u003eThis storage solution is suitable for studies involving gastrointestinal tissue biology, cellular composition, and disease-related tissue changes.\u003c\/p\u003e\n\u003cul data-start=\"2083\" data-end=\"2370\"\u003e\n\u003cli data-section-id=\"1w1fmae\" data-start=\"2083\" data-end=\"2109\"\u003eGastrointestinal biology\u003c\/li\u003e\n\u003cli data-section-id=\"5bfczm\" data-start=\"2110\" data-end=\"2130\"\u003eMucosal immunology\u003c\/li\u003e\n\u003cli data-section-id=\"1dlt8en\" data-start=\"2131\" data-end=\"2159\"\u003eDigestive disease research\u003c\/li\u003e\n\u003cli data-section-id=\"1qdbd06\" data-start=\"2160\" data-end=\"2186\"\u003eGut inflammation studies\u003c\/li\u003e\n\u003cli data-section-id=\"7bli9g\" data-start=\"2187\" data-end=\"2239\"\u003eMicroenvironment and tissue heterogeneity research\u003c\/li\u003e\n\u003cli data-section-id=\"q51jok\" data-start=\"2240\" data-end=\"2270\"\u003eAnimal disease model studies\u003c\/li\u003e\n\u003cli data-section-id=\"82chcw\" data-start=\"2271\" data-end=\"2292\"\u003eSingle-cell biology\u003c\/li\u003e\n\u003cli data-section-id=\"10aof08\" data-start=\"2293\" data-end=\"2328\"\u003eTranslational biomedical research\u003c\/li\u003e\n\u003cli data-section-id=\"vaibvg\" data-start=\"2329\" data-end=\"2370\"\u003eDrug discovery and preclinical research\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 data-section-id=\"1fuj4ew\" data-start=\"2372\" data-end=\"2392\"\u003eKey Applications\u003c\/h3\u003e\n\u003cp data-start=\"2394\" data-end=\"2493\"\u003eThe solution supports short-term sample preservation before downstream tissue-processing workflows.\u003c\/p\u003e\n\u003cul data-start=\"2495\" data-end=\"2876\"\u003e\n\u003cli data-section-id=\"16lfw7y\" data-start=\"2495\" data-end=\"2551\"\u003ePreservation of ex vivo animal gastrointestinal tissue\u003c\/li\u003e\n\u003cli data-section-id=\"1nmm01o\" data-start=\"2552\" data-end=\"2595\"\u003eSample transport under 2°C–8°C conditions\u003c\/li\u003e\n\u003cli data-section-id=\"tnqfd6\" data-start=\"2596\" data-end=\"2650\"\u003ePreparation before single-cell suspension generation\u003c\/li\u003e\n\u003cli data-section-id=\"zt9u16\" data-start=\"2651\" data-end=\"2701\"\u003eHigh-throughput single-cell sequencing workflows\u003c\/li\u003e\n\u003cli data-section-id=\"19krc79\" data-start=\"2702\" data-end=\"2756\"\u003eCell viability maintenance during short-term storage\u003c\/li\u003e\n\u003cli data-section-id=\"ptacj0\" data-start=\"2757\" data-end=\"2818\"\u003eTissue handling for gastrointestinal disease model research\u003c\/li\u003e\n\u003cli data-section-id=\"15b2hal\" data-start=\"2819\" data-end=\"2876\"\u003ePre-processing support for downstream cellular analysis\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003eSpecifications\u003c\/h2\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eAnimal Gastrointestinal Tissue Storage Solution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalog No.\u003c\/td\u003e\n\u003ctd\u003eFG-BA3350\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePack Size\u003c\/td\u003e\n\u003ctd\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eComponent Name\u003c\/td\u003e\n\u003ctd\u003eAGTSS, Animal Gastrointestinal Tissue Storage Solution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Type\u003c\/td\u003e\n\u003ctd\u003eAnimal gastrointestinal tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible Samples\u003c\/td\u003e\n\u003ctd\u003eFresh ex vivo gastrointestinal tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended Tissue Amount\u003c\/td\u003e\n\u003ctd\u003eApproximately 200 mg tissue per 2 mL cryovial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Function\u003c\/td\u003e\n\u003ctd\u003eShort-term preservation of ex vivo animal gastrointestinal tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWorkflow\u003c\/td\u003e\n\u003ctd\u003eTissue washing, immersion in storage solution, low-temperature storage or transport, downstream processing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage During Sample Preservation\u003c\/td\u003e\n\u003ctd\u003e2°C–8°C; avoid freezing samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended Processing Window\u003c\/td\u003e\n\u003ctd\u003eWithin 48 hours after preservation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDownstream Applications\u003c\/td\u003e\n\u003ctd\u003eCell suspension preparation, high-throughput single-cell sequencing, related cell-based research\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Reagents\u003c\/td\u003e\n\u003ctd\u003ePBS buffer solution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Tissue Tools\u003c\/td\u003e\n\u003ctd\u003eSurgical scissors, scalpels, forceps\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Consumables\u003c\/td\u003e\n\u003ctd\u003eLow-adsorption pipette tips, 2 mL cryovials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Storage\u003c\/td\u003e\n\u003ctd\u003e-20°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf Life\u003c\/td\u003e\n\u003ctd\u003eOne year\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHandling Note\u003c\/td\u003e\n\u003ctd\u003eAliquot promptly after receipt to avoid repeated freeze-thaw cycles\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eResearch Use\u003c\/td\u003e\n\u003ctd\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003eKit Components\u003c\/h2\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eCatalog Number\u003c\/th\u003e\n\u003cth align=\"right\"\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAGTSS, Animal Gastrointestinal Tissue Storage Solution\u003c\/td\u003e\n\u003ctd\u003eFG-BA3350-A\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"FireGene","offers":[{"title":"100 mL","offer_id":47868393062612,"sku":"FG-BA3350-100","price":279.0,"currency_code":"USD","in_stock":true},{"title":"500 mL","offer_id":47868393095380,"sku":"FG-BA3350-500","price":739.0,"currency_code":"USD","in_stock":true}]},{"product_id":"serum-free-cell-freezing-medium","title":"FireGene Serum-Free One-Step Cell Freezing Medium for Cell Cryopreservation","description":"\u003cp\u003e\u003cstrong\u003eFireGene Serum-Free One-Step Cell Freezing Medium\u003c\/strong\u003e is designed for the cryopreservation of animal primary cells and cell lines, especially cells that require a clear culture background or are sensitive to animal-origin components and DMSO. The formulation is serum-free, animal-origin-free, antibiotic-free, non-toxic, and contains low DMSO. The workflow does not require programmed cooling; cells can be resuspended in the freezing medium, aliquoted into cryovials, and placed directly at -80°C before optional transfer to liquid nitrogen for longer-term storage.\u003c\/p\u003e\n\u003ch2\u003eBackground Information\u003c\/h2\u003e\n\u003cp data-start=\"1241\" data-end=\"1554\"\u003eCell freezing medium is used to protect cells during low-temperature storage and help preserve cell recovery after thawing. For many research workflows, serum-free and animal-origin-free formulations are preferred because they reduce undefined biological components and help maintain a clearer culture background.\u003c\/p\u003e\n\u003cp data-start=\"1556\" data-end=\"1742\"\u003e\u003cstrong data-start=\"1556\" data-end=\"1609\"\u003eFireGene Serum-Free One-Step Cell Freezing Medium\u003c\/strong\u003e is suitable for animal primary cells and cell lines that require simplified, serum-free cryopreservation without programmed cooling.\u003c\/p\u003e\n\u003ch3 data-section-id=\"15x6sgr\" data-start=\"1744\" data-end=\"1762\"\u003eResearch Areas\u003c\/h3\u003e\n\u003cp data-start=\"1764\" data-end=\"1889\"\u003eThis freezing medium is suitable for laboratories working with primary cells, cultured cell lines, and sensitive cell models.\u003c\/p\u003e\n\u003cul data-start=\"1891\" data-end=\"2145\"\u003e\n\u003cli data-section-id=\"1wbfad3\" data-start=\"1891\" data-end=\"1905\"\u003eCell biology\u003c\/li\u003e\n\u003cli data-section-id=\"1x0kd5n\" data-start=\"1906\" data-end=\"1929\"\u003ePrimary cell research\u003c\/li\u003e\n\u003cli data-section-id=\"r3ap7s\" data-start=\"1930\" data-end=\"1942\"\u003eImmunology\u003c\/li\u003e\n\u003cli data-section-id=\"143grux\" data-start=\"1943\" data-end=\"1960\"\u003eCancer research\u003c\/li\u003e\n\u003cli data-section-id=\"stme50\" data-start=\"1961\" data-end=\"2007\"\u003eStem cell and regenerative medicine research\u003c\/li\u003e\n\u003cli data-section-id=\"1w8a9b5\" data-start=\"2008\" data-end=\"2038\"\u003eDrug discovery and screening\u003c\/li\u003e\n\u003cli data-section-id=\"18a434y\" data-start=\"2039\" data-end=\"2074\"\u003eCell culture workflow development\u003c\/li\u003e\n\u003cli data-section-id=\"10aof08\" data-start=\"2075\" data-end=\"2110\"\u003eTranslational biomedical research\u003c\/li\u003e\n\u003cli data-section-id=\"sn29lg\" data-start=\"2111\" data-end=\"2145\"\u003eBiobanking and sample management\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 data-section-id=\"1fuj4ew\" data-start=\"2147\" data-end=\"2167\"\u003eKey Applications\u003c\/h3\u003e\n\u003cp data-start=\"2169\" data-end=\"2246\"\u003eThe product supports routine and specialized cell cryopreservation workflows.\u003c\/p\u003e\n\u003cul data-start=\"2248\" data-end=\"2580\"\u003e\n\u003cli data-section-id=\"ljcpsh\" data-start=\"2248\" data-end=\"2290\"\u003eCryopreservation of animal primary cells\u003c\/li\u003e\n\u003cli data-section-id=\"1rbnjbh\" data-start=\"2291\" data-end=\"2342\"\u003eCryopreservation of established animal cell lines\u003c\/li\u003e\n\u003cli data-section-id=\"fwfv2l\" data-start=\"2343\" data-end=\"2368\"\u003eSerum-free cell banking\u003c\/li\u003e\n\u003cli data-section-id=\"1ha4b0y\" data-start=\"2369\" data-end=\"2417\"\u003eShort-term or long-term cell storage workflows\u003c\/li\u003e\n\u003cli data-section-id=\"1xfdjj5\" data-start=\"2418\" data-end=\"2458\"\u003eCell recovery before culture expansion\u003c\/li\u003e\n\u003cli data-section-id=\"15lwr3b\" data-start=\"2459\" data-end=\"2506\"\u003eCell storage for downstream functional assays\u003c\/li\u003e\n\u003cli data-section-id=\"2i9zwn\" data-start=\"2507\" data-end=\"2580\"\u003eCryopreservation of cells sensitive to animal-origin components or DMSO\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003eSpecifications\u003c\/h2\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eSerum-Free One-Step Cell Freezing Medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalog No.\u003c\/td\u003e\n\u003ctd\u003eFG-BA3356\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePack Size\u003c\/td\u003e\n\u003ctd\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFormulation Features\u003c\/td\u003e\n\u003ctd\u003eSerum-free, animal-origin-free, antibiotic-free, low DMSO\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible Samples\u003c\/td\u003e\n\u003ctd\u003eAnimal primary cells and cell lines\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Function\u003c\/td\u003e\n\u003ctd\u003eCell cryopreservation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWorkflow\u003c\/td\u003e\n\u003ctd\u003eCell counting, centrifugation, resuspension in freezing medium, cryovial aliquoting, -80°C freezing, optional liquid nitrogen storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProgrammed Cooling Required\u003c\/td\u003e\n\u003ctd\u003eNo\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eGeneral Cryopreservation Density\u003c\/td\u003e\n\u003ctd\u003e1 × 10⁶ cells\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage After Freezing Cells\u003c\/td\u003e\n\u003ctd\u003eDirectly place cryovials at -80°C; transfer to liquid nitrogen after 24 hours for longer-term storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eThawing Temperature\u003c\/td\u003e\n\u003ctd\u003e37°C water bath\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eExample Thawing Centrifugation\u003c\/td\u003e\n\u003ctd\u003e300 ×g for 5 minutes at 4°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Instruments for Freezing\u003c\/td\u003e\n\u003ctd\u003eHorizontal centrifuge, cell counter, -80°C freezer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Instruments for Thawing\u003c\/td\u003e\n\u003ctd\u003eHorizontal centrifuge, water bath\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Reagents for Thawing\u003c\/td\u003e\n\u003ctd\u003eRPMI 1640 medium or suitable culture medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Consumables\u003c\/td\u003e\n\u003ctd\u003eCell cryovials, low-adsorption pipette tips, centrifuge tubes, alcohol cotton balls\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Storage\u003c\/td\u003e\n\u003ctd\u003e-20°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf Life\u003c\/td\u003e\n\u003ctd\u003eOne year\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eResearch Use\u003c\/td\u003e\n\u003ctd\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003eKit Components\u003c\/h2\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eCatalog Number\u003c\/th\u003e\n\u003cth align=\"right\"\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eSerum-Free One-Step Cell Freezing Medium\u003c\/td\u003e\n\u003ctd\u003eBA3356\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"FireGene","offers":[{"title":"100 mL","offer_id":47868396241108,"sku":"FG-BA3356-100","price":199.0,"currency_code":"USD","in_stock":true},{"title":"500 mL","offer_id":47868396273876,"sku":"FG-BA3356-500","price":389.0,"currency_code":"USD","in_stock":true}]},{"product_id":"serum-free-dmso-free-cell-freezing-medium","title":"FireGene Serum-Free and DMSO-Free Cell Freezing Medium for Cell Cryopreservation","description":"\u003cp\u003e\u003cstrong\u003eFireGene Serum-Free and DMSO-Free Cell Freezing Medium\u003c\/strong\u003e is designed for cryopreservation of animal primary cells and immortalized cell lines that are sensitive to animal-origin components or DMSO. The formulation is serum-free, animal-origin-free, DMSO-free, and non-toxic. Cells are resuspended in the freezing medium, aliquoted into cryovials, placed in a programmable freezing container for controlled-rate cooling at approximately 1°C\/min, and stored at -80°C before optional transfer to liquid nitrogen for longer-term storage.\u003c\/p\u003e\n\u003ch2\u003eBackground Information\u003c\/h2\u003e\n\u003cp data-end=\"1577\" data-start=\"1237\"\u003eDMSO is commonly used in cell freezing media, but some primary cells and sensitive cell lines may require alternatives that avoid DMSO and animal-origin components. A serum-free and DMSO-free freezing medium can help support cell banking workflows where a clearer culture background or reduced exposure to undefined components is preferred.\u003c\/p\u003e\n\u003cp data-end=\"1788\" data-start=\"1579\"\u003e\u003cstrong data-end=\"1637\" data-start=\"1579\"\u003eFireGene Serum-Free and DMSO-Free Cell Freezing Medium\u003c\/strong\u003e provides a defined cryopreservation option for animal primary cells and immortalized cell lines that are sensitive to DMSO or animal-origin materials.\u003c\/p\u003e\n\u003ch3 data-end=\"1808\" data-start=\"1790\" data-section-id=\"15x6sgr\"\u003eResearch Areas\u003c\/h3\u003e\n\u003cp data-end=\"1923\" data-start=\"1810\"\u003eThis freezing medium is suitable for cell culture and cryopreservation workflows across multiple research fields.\u003c\/p\u003e\n\u003cul data-end=\"2191\" data-start=\"1925\"\u003e\n\u003cli data-end=\"1939\" data-start=\"1925\" data-section-id=\"1wbfad3\"\u003eCell biology\u003c\/li\u003e\n\u003cli data-end=\"1963\" data-start=\"1940\" data-section-id=\"1x0kd5n\"\u003ePrimary cell research\u003c\/li\u003e\n\u003cli data-end=\"1976\" data-start=\"1964\" data-section-id=\"r3ap7s\"\u003eImmunology\u003c\/li\u003e\n\u003cli data-end=\"1994\" data-start=\"1977\" data-section-id=\"143grux\"\u003eCancer research\u003c\/li\u003e\n\u003cli data-end=\"2041\" data-start=\"1995\" data-section-id=\"stme50\"\u003eStem cell and regenerative medicine research\u003c\/li\u003e\n\u003cli data-end=\"2072\" data-start=\"2042\" data-section-id=\"1w8a9b5\"\u003eDrug discovery and screening\u003c\/li\u003e\n\u003cli data-end=\"2108\" data-start=\"2073\" data-section-id=\"10aof08\"\u003eTranslational biomedical research\u003c\/li\u003e\n\u003cli data-end=\"2143\" data-start=\"2109\" data-section-id=\"buxy7j\"\u003eCell banking and biopreservation\u003c\/li\u003e\n\u003cli data-end=\"2191\" data-start=\"2144\" data-section-id=\"18prr6a\"\u003eAssay development using sensitive cell models\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 data-end=\"2213\" data-start=\"2193\" data-section-id=\"1fuj4ew\"\u003eKey Applications\u003c\/h3\u003e\n\u003cp data-end=\"2340\" data-start=\"2215\"\u003eThe product supports routine and specialized cryopreservation workflows where serum-free and DMSO-free conditions are needed.\u003c\/p\u003e\n\u003cul data-end=\"2694\" data-start=\"2342\"\u003e\n\u003cli data-end=\"2384\" data-start=\"2342\" data-section-id=\"ljcpsh\"\u003eCryopreservation of animal primary cells\u003c\/li\u003e\n\u003cli data-end=\"2437\" data-start=\"2385\" data-section-id=\"16be5n0\"\u003eCryopreservation of immortalized animal cell lines\u003c\/li\u003e\n\u003cli data-end=\"2463\" data-start=\"2438\" data-section-id=\"7tf4a4\"\u003eDMSO-free cell freezing\u003c\/li\u003e\n\u003cli data-end=\"2489\" data-start=\"2464\" data-section-id=\"fwfv2l\"\u003eSerum-free cell banking\u003c\/li\u003e\n\u003cli data-end=\"2551\" data-start=\"2490\" data-section-id=\"1oiz1hk\"\u003ePreservation of cells sensitive to animal-origin components\u003c\/li\u003e\n\u003cli data-end=\"2592\" data-start=\"2552\" data-section-id=\"1xfdjj5\"\u003eCell recovery before culture expansion\u003c\/li\u003e\n\u003cli data-end=\"2646\" data-start=\"2593\" data-section-id=\"1yfj1k2\"\u003eLong-term storage after transfer to liquid nitrogen\u003c\/li\u003e\n\u003cli data-end=\"2694\" data-start=\"2647\" data-section-id=\"15lwr3b\"\u003eCell storage for downstream functional assays\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003eSpecifications\u003c\/h2\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eSerum-Free and DMSO-Free Cell Freezing Medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalog No.\u003c\/td\u003e\n\u003ctd\u003eFG-BA3341\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePack Size\u003c\/td\u003e\n\u003ctd\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFormulation Features\u003c\/td\u003e\n\u003ctd\u003eSerum-free, animal-origin-free, DMSO-free, non-toxic\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible Samples\u003c\/td\u003e\n\u003ctd\u003eAnimal primary cells and immortalized cell lines\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Function\u003c\/td\u003e\n\u003ctd\u003eCell cryopreservation and preservation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWorkflow\u003c\/td\u003e\n\u003ctd\u003eCell counting, centrifugation, resuspension in freezing medium, cryovial aliquoting, controlled-rate cooling, -80°C storage, optional liquid nitrogen transfer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eControlled-Rate Cooling\u003c\/td\u003e\n\u003ctd\u003eRecommended; approximately 1°C\/min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eGeneral Cryopreservation Density\u003c\/td\u003e\n\u003ctd\u003e1 × 10⁶ cells\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage After Freezing Cells\u003c\/td\u003e\n\u003ctd\u003e-80°C initially; transfer to liquid nitrogen after 24 hours for longer-term storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eThawing Temperature\u003c\/td\u003e\n\u003ctd\u003e37°C water bath\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eExample Thawing Centrifugation\u003c\/td\u003e\n\u003ctd\u003e300 ×g for 5 minutes at 4°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Instruments for Freezing\u003c\/td\u003e\n\u003ctd\u003eHorizontal centrifuge, cell counter, -80°C freezer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Instruments for Thawing\u003c\/td\u003e\n\u003ctd\u003eHorizontal centrifuge, water bath\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Reagents for Thawing\u003c\/td\u003e\n\u003ctd\u003eRPMI 1640 medium or suitable culture medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Consumables\u003c\/td\u003e\n\u003ctd\u003eCell cryovials, programmable freezing container, low-adsorption pipette tips, centrifuge tubes, alcohol cotton balls\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Storage\u003c\/td\u003e\n\u003ctd\u003e-20°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf Life\u003c\/td\u003e\n\u003ctd\u003eOne year\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eResearch Use\u003c\/td\u003e\n\u003ctd\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003eKit Components\u003c\/h2\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eCatalog Number\u003c\/th\u003e\n\u003cth align=\"right\"\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eSerum-Free and DMSO-Free Cell Freezing Medium\u003c\/td\u003e\n\u003ctd\u003eFG-BA3341\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"FireGene","offers":[{"title":"100 mL","offer_id":47868397355220,"sku":"FG-BA3341-100","price":199.0,"currency_code":"USD","in_stock":true},{"title":"500 mL","offer_id":47868397387988,"sku":"FG-BA3341-500","price":389.0,"currency_code":"USD","in_stock":true}]}],"url":"https:\/\/firegene.com\/collections\/cell-and-tissue-preservation-for-single-cell-research.oembed","provider":"FireGene","version":"1.0","type":"link"}