{"title":"Cloning and Mutagenesis Kits","description":"\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003cstrong\u003eCloning and Mutagenesis Kits\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003eRestriction enzyme cloning works until the restriction site you need is already in your insert. Overlap extension PCR mutagenesis works until the fragment is too long and the error rate too high to trust the sequence. Both are still common in molecular biology labs — and both have a faster replacement that doesn't require gel purification between steps.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003eThis collection covers two kits built around homologous recombination rather than ligation or annealing-based chemistry.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003cstrong\u003eOne Step Cloning Kit — seamless directional cloning\u003c\/strong\u003e Built on UvsXase, a recombinase derived from T4 phage. Directionally inserts PCR products into any linearized or restriction-digested vector at any site — restriction fragments and PCR-linearized vectors work directly, with no DNA purification required before the recombination reaction. Homology arms of 15–20 bp added to insert primers are sufficient for efficient directional cloning. Suitable for single-fragment insertion, multi-fragment assembly, and high-throughput cloning workflows. \u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003cstrong\u003eFast Site-Directed Mutagenesis Kit — whole-plasmid amplification and recombination\u003c\/strong\u003e Two-module system: a high-fidelity Max Master Mix amplification module for whole-plasmid PCR up to 20 kb, and a rapid homologous recombination module for circularization. DpnI digestion removes the original methylated template; the amplified product goes directly into the recombination reaction without gel purification. Introduces point mutations, insertions, or deletions at any position in plasmids under 20 kb in a single round of amplification. \u003c\/p\u003e","products":[{"product_id":"firegene-fast-site-directed-mutagenesis-kits","title":"FireGene Fast Site-Directed Mutagenesis Kits","description":"\u003cp\u003eThe FireGene Fast Mutagenesis Kit is a site-directed mutagenesis system based on our rapid cloning technology. Using this kit, the target plasmid amplification product is be retreived by gel or DpnI digestion, the site-directed mutagenesis can be accomplished by direct transformation after recombination circularization. The kit consists of two modules: the Max Master Mix Amplification Module and the Rapid Cloning Module. The ultra-high fidelity of Max Master Mix significantly reduces the possibility of introducing new mutations during the amplification process, and it is conducive to the complete degradation of the original methylated template due to the extremely low amount of template used. Its excellent ability to amplify long fragments is widely applicable to the amplification of any plasmids less than 20 kb in length. The rapid cloning system utilizes an efficient homologous recombination reaction, so that when the site-directed mutagenesis kit performs DNA site-directed mutagenesis, the linearized PCR product can be efficiently circularized, which is beneficial to transform the host and improve the mutation efficiency.\u003c\/p\u003e\n\u003ch4\u003eApplications\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDirectional cloning into virtually any vector at any site\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/h4\u003e\n\u003ctable style=\"width: 100%;\" data-mce-style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 60%;\" data-mce-style=\"width: 60%;\"\u003e\u003cstrong\u003eComponent Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%;\" data-mce-style=\"width: 20%;\"\u003e\u003cstrong\u003eAmount\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%;\" data-mce-style=\"width: 20%;\"\u003e\u003cstrong\u003eStorage (°C)\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003e\u003cspan\u003eDpnI\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e20 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003e\u003cspan\u003eUvsXase\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e20 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003e\u003cspan\u003eUFO Buffer\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e40 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003eRestrictions\u003cbr\u003e\n\u003c\/h4\u003e\n\u003cp\u003e\u003cspan\u003eFor Research or Educational Use Only.\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv id=\"gtx-trans\" style=\"position: absolute; left: 38px; top: 382.55px;\"\u003e\n\u003cdiv class=\"gtx-trans-icon\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42896457367764,"sku":"FG-C00201","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder_4d74ec5e-476c-4631-bc3c-ec7e1e6b0062.jpg?v=1656611399"},{"product_id":"firegene-one-step-cloning-kit","title":"FireGene One Step Cloning Kit","description":"\u003cp data-mce-fragment=\"1\"\u003eFireGene One Step Cloning technology is a Fast, Efficient, and simple DNA directional cloning technology, which can directionally clone the PCR product of the target gene to any site of any vector. FireGene One Step Cloning Kit is a new generation recombination cloning kit with the core of recombinase obtained by heterologous expression of a gene of T4 phage. UvsXase is compatible with conventional digestion and PCR reaction systems. Cloning vector restriction products or PCR products and target gene PCR products can be directly used for recombinant cloning without DNA purification, which greatly simplifies the experimental steps.\u003c\/p\u003e\n\u003ch4 data-mce-fragment=\"1\"\u003eApplications\u003c\/h4\u003e\n\u003cul data-mce-fragment=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003eDNA site-directed mutagenesis\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003eHigh-throughput cloning\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003eSeamless cloning\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003eFast cloning\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4 data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eComponents\u003c\/span\u003e\u003c\/h4\u003e\n\u003ctable style=\"width: 100%;\" data-mce-fragment=\"1\" data-mce-style=\"width: 100%;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 60%;\" data-mce-fragment=\"1\" data-mce-style=\"width: 60%;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eComponent Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%;\" data-mce-fragment=\"1\" data-mce-style=\"width: 20%;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eAmount\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%;\" data-mce-fragment=\"1\" data-mce-style=\"width: 20%;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage (°C)\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 50%;\" data-mce-fragment=\"1\" data-mce-style=\"width: 50%;\"\u003e\u003cspan\u003eUvsXase\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-fragment=\"1\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e50 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-fragment=\"1\" data-mce-style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 50%;\" data-mce-fragment=\"1\" data-mce-style=\"width: 50%;\"\u003e\u003cspan\u003e5 xUFO Buffer\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-fragment=\"1\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e100 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-fragment=\"1\" data-mce-style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003ch4 data-mce-fragment=\"1\"\u003eExperiment Workflow\u003c\/h4\u003e\n\u003cp\u003eBasic Steps:\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eAmplify the whole plasmid of the vector at the cloning site to obtain a linearized vector;\u003c\/li\u003e\n\u003cli\u003eDesign homologous primers so that the 5' and 3' ends of the PCR product of the target fragment have the c\u003cspan data-mce-fragment=\"1\"\u003eompletely identical sequence as the \u003c\/span\u003efront and rear ends of the pre-inserted linearized vector, respectively (15 bp ~ 20 bp);\u003c\/li\u003e\n\u003cli\u003eAfter mixing the amplified PCR product of the target gene and the linearized vector in a certain proportion, under the catalysis of UvsXase, the transformation can be carried out within 30 minutes, and the directional cloning is completed.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eThe positive rate of clones can reach more than 95%.\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"Flow chart of single gene cloning experiment using UvsXase\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/files\/Flow_chart_of_single_gene_cloning_experiment_using_UvsXase_480x480.png?v=1654717278\"\u003e\u003c\/p\u003e\n\u003ch4 data-mce-fragment=\"1\"\u003eRestrictions\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/h4\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eFor Research or Educational Use Only.\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42896681664724,"sku":"FG-C00101","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder_b50f057f-0f30-482d-9abf-ca2bc6443b42.jpg?v=1656611716"}],"url":"https:\/\/firegene.com\/collections\/cloning-and-mutagenesis-kits.oembed","provider":"FireGene","version":"1.0","type":"link"}