{"title":"Liquid Biopsy","description":"\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003cstrong\u003eLiquid Biopsy\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003eA liquid biopsy is only as sensitive as the weakest step in the workflow. ctDNA can make up less than 0.1% of total cfDNA in early-stage cancer — which means a suboptimal extraction, a PCR duplication artifact, or a library prep that loses short fragments can cost you the signal you were looking for.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003eThis page brings together FireGene products validated for liquid biopsy workflows, organized by where they sit in the pre-analytical and library preparation pipeline.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003cstrong\u003ecfDNA Extraction\u003c\/strong\u003e Two column-based options for plasma cfDNA isolation from 1–4 mL plasma input. The Plasma cfDNA Extraction Kit and Circulating Free DNA Extraction Kit are optimized for the short, highly fragmented nature of cfDNA — the 160–200 bp mononucleosomal fragments that standard genomic DNA extraction protocols systematically lose. Both deliver inhibitor-free, high-purity cfDNA compatible with ddPCR, targeted NGS panels, and whole-genome approaches.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003cstrong\u003ePCR-Free Library Preparation\u003c\/strong\u003e The Non-Amplification DNA Library Kit for Illumina skips PCR amplification entirely — the step that introduces the duplication bias that distorts variant allele frequency measurements at low ctDNA concentrations. For cfDNA inputs where every molecule counts, removing PCR preserves the true representation of the circulating nucleic acid pool.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003cstrong\u003eDNA Methylation Sequencing\u003c\/strong\u003e The Post-Bisulfite DNA Library Preparation Kit supports whole-genome bisulfite sequencing (WGBS) and reduced-representation methylation profiling from cfDNA — the foundation of methylation-based liquid biopsy approaches for tissue-of-origin classification and early cancer detection.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003eFor complete workflow guidance, contact us directly.\u003c\/p\u003e","products":[{"product_id":"firegene-plasma-cfdna-extraction-kit","title":"FireGene Plasma cfDNA Extraction Kit","description":"\u003ch4 class=\"font_9\"\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp class=\"font_9\"\u003eThe kit employed the FireGene-developed magnetic bead purification technology and unique buffer system, which is suitable \u003cspan\u003eto extract cell-free DNA (cfDNA) \u003c\/span\u003efrom large-volume (1 mL~4mL) EDTA plasma, cell-free DNA sample preservation tube plasma, such as Streck Cell-Free DNA BCT non-invasive blood collection tube, CWBIO's cell-free DNA sample storage tubes, etc. The extraction process does not involve toxic reagents such as chloroform and phenol. The extracted cfDNA is stable and reliable quality with\u003cspan\u003e high purity. The extracted cfDNA\u003c\/span\u003e can be used for conventional PCR, quantitative PCR, chip detection, downstream experiments such as high-throughput sequencing. The kit can also be efficiently used with the pipetting workstation and \u003cspan\u003emagnetic bar method magnetic bead automatic extraction system\u003c\/span\u003e.\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch4 data-uw-styling-context=\"true\"\u003e\n\u003cbr\u003e\u003cmeta charset=\"utf-8\"\u003e\n\u003c\/h4\u003e\n\u003ch4 data-uw-styling-context=\"true\"\u003eSpecifications\u003c\/h4\u003e\n\u003ctable width=\"100%\" style=\"width: 100%; height: 400.688px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 23.3684%; height: 19.5938px;\"\u003e\u003cstrong\u003eProduct Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 76.2706%; height: 19.5938px;\"\u003e\u003cspan\u003eNucleic Acid Extraction\/Purification\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 58.7812px;\"\u003e\n\u003ctd style=\"width: 23.3684%; height: 58.7812px;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cstrong\u003eApplications\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 76.2706%; height: 58.7812px;\"\u003e\n\u003cspan\u003eConventional PCR, quantitative PCR, chip detection, downstream experiments such as high-throughput sequencing\u003c\/span\u003e\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.1875px;\"\u003e\n\u003ctd style=\"width: 23.3684%; height: 39.1875px;\"\u003e\u003cstrong\u003eCompatible Sample Types\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 76.2706%; height: 39.1875px;\"\u003e\u003cspan\u003eEDTA plasma, cell-free DNA sample preservation tube plasma, etc.\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 23.3684%; height: 19.5938px;\"\u003e\u003cstrong\u003eTarget\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 76.2706%; height: 19.5938px;\"\u003e\u003cspan\u003ecfDNA\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 23.3684%; height: 19.5938px;\"\u003e\u003cb\u003eMethod\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 76.2706%; height: 19.5938px;\"\u003e\u003cspan\u003eMagnetic beads\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 23.3684%; height: 19.5938px;\"\u003e\u003cstrong\u003ePrep Time\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 76.2706%; height: 19.5938px;\"\u003e\u003cbr\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.1875px;\"\u003e\n\u003ctd style=\"width: 23.3684%; height: 39.1875px;\"\u003e\u003cstrong\u003eAmount of Starting Material\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 76.2706%; height: 39.1875px;\"\u003e\u003cbr\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.1875px;\"\u003e\n\u003ctd style=\"width: 23.3684%; height: 39.1875px;\"\u003e\u003cstrong\u003eSupported Instruments\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 76.2706%; height: 39.1875px;\"\u003eCompatible with most automated nucleic acid extraction instruments, high-throughput compatible\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 23.3684%; height: 19.5938px;\"\u003e\u003cstrong\u003eNumber of Reactions\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 76.2706%; height: 19.5938px;\"\u003e\u003cspan\u003e48 rxns\/96rxns\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 106.781px;\"\u003e\n\u003ctd style=\"width: 23.3684%; height: 106.781px;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 76.2706%; height: 106.781px;\"\u003e\n\u003cp\u003e\u003cspan\u003e-\u003cspan style=\"font-family: Times New Roman;\"\u003eProteinase K and magnetic bead suspension should be stored at C2-8\u003c\/span\u003e\u003cspan style=\"font-family: 宋体;\"\u003e℃\u003c\/span\u003e\u003cspan style=\"font-family: Times New Roman;\"\u003e; other components should be stored at room temperature\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 23.3684%; height: 19.5938px;\"\u003e\u003cb\u003eShelf-life\u003c\/b\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 76.2706%; height: 19.5938px;\"\u003e\u003cspan\u003e12 months\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 id=\"detection-principle\"\u003eKit Components\u003c\/h3\u003e\n\u003ctable class=\"MsoNormalTable\" border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"254\" valign=\"center\"\u003e\n\u003cp class=\"16\" align=\"center\"\u003e\u003cb\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e Name\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"121\" valign=\"center\"\u003e\n\u003cp class=\"16\" align=\"center\"\u003e\u003cb\u003e\u003cspan\u003e10 times\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"174\" valign=\"center\"\u003e\n\u003cp class=\"16\" align=\"center\"\u003e\u003cb\u003e\u003cspan\u003eStorage temperature\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"254\" valign=\"center\"\u003e\n\u003cp class=\"16\" align=\"center\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Times New Roman;\"\u003eL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eysis buffer 1\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"121\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Times New Roman;\"\u003e4.5\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"174\" valign=\"center\"\u003e\n\u003cp class=\"16\" align=\"center\"\u003e\u003cspan\u003eRT.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"254\" valign=\"center\"\u003e\n\u003cp class=\"16\" align=\"center\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Times New Roman;\"\u003eL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eysis buffer \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Times New Roman;\"\u003e2\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"121\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Times New Roman;\"\u003e33.6\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"174\" valign=\"center\"\u003e\n\u003cp class=\"16\" align=\"center\"\u003e\u003cspan\u003eRT.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"254\" valign=\"center\"\u003e\n\u003cp class=\"16\" align=\"center\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Times New Roman;\"\u003eP\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eroteinase K\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"121\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Times New Roman;\"\u003e1.7mL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"174\" valign=\"center\"\u003e\n\u003cp class=\"16\" align=\"center\"\u003e\u003cspan\u003e2-8℃\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"254\" valign=\"center\"\u003e\n\u003cp class=\"16\" align=\"center\"\u003e\u003cspan\u003eMagnetic bead suspension C\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"121\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Times New Roman;\"\u003e0.9\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"174\" valign=\"center\"\u003e\n\u003cp class=\"16\" align=\"center\"\u003e\u003cspan\u003e2-8℃\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"254\" valign=\"center\"\u003e\n\u003cp class=\"16\" align=\"center\"\u003e\u003cspan\u003eWashing solution C1\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"121\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Times New Roman;\"\u003e11\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"174\" valign=\"center\"\u003e\n\u003cp class=\"16\" align=\"center\"\u003e\u003cspan\u003eRT.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"254\" valign=\"center\"\u003e\n\u003cp class=\"16\" align=\"center\"\u003e\u003cspan\u003eWashing liquid C2\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"121\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Times New Roman;\"\u003e5\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003emL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"174\" valign=\"center\"\u003e\n\u003cp class=\"16\" align=\"center\"\u003e\u003cspan\u003eRT.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"254\" valign=\"center\"\u003e\n\u003cp class=\"16\" align=\"center\"\u003e\u003cspan\u003eElution Buffer C\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"121\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"center\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Times New Roman;\"\u003e800\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Times New Roman;\"\u003eµL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"174\" valign=\"center\"\u003e\n\u003cp class=\"16\" align=\"center\"\u003e\u003cspan\u003eRT.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv id=\"gtx-trans\" style=\"position: absolute; left: 169px; top: 359.784px;\"\u003e\n\u003cdiv class=\"gtx-trans-icon\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e","brand":"FireGene","offers":[{"title":"10 reactions\/kit","offer_id":47833609502932,"sku":"FG-FG1102D-10rxns","price":69.0,"currency_code":"USD","in_stock":true},{"title":"48  reactions\/kit","offer_id":47722166747348,"sku":"FG-FG1102D-48rxns","price":249.0,"currency_code":"USD","in_stock":true},{"title":"96  reactions\/kit","offer_id":46130500534484,"sku":"FG-FG1102D-96rxns","price":419.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/PlasmacfDNAExtractionKit.jpg?v=1648852571"},{"product_id":"firegene-circulating-free-dna-extraction-kit-columns","title":"FireGene Circulating Free DNA Extraction Kit (Columns)","description":"\u003cp\u003eThe Kit is designed for rapid and efficient purification of circulating cell free DNA (cfDNA). The system utilizes silicacoated paramagnetic particles to purify cfDNA from less than 0.3 mL to greater than 5 mL of serum or plasma. The buffers were developed to ensure efficient recovery of 100 bp - 500 bp DNA fragments in order to maximize recovery of cfDNA. The recovered DNA is suitable for a wide range of downstream applications, such as bisulfite sequencing, NGS, and qPCR.\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42967998529748,"sku":"FG-0315","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder_8074d57a-58c0-4150-a7d0-07a399f6aadf.jpg?v=1656610878"}],"url":"https:\/\/firegene.com\/collections\/liquid-biopsy.oembed","provider":"FireGene","version":"1.0","type":"link"}