{"title":"Neuroscience Single-Cell Sample Prep","description":"\u003cdiv\u003e\n\u003cdiv class=\"standard-markdown grid-cols-1 grid [\u0026amp;_\u0026gt;_*]:min-w-0 gap-3\"\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003cstrong\u003eNeuroscience Single-Cell Sample Prep\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"standard-markdown grid-cols-1 grid [\u0026amp;_\u0026gt;_*]:min-w-0 gap-3\"\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003eBrain tissue doesn't dissociate like liver or lung. A protocol that works on soft tissue will shear neurons, leave myelin debris in suspension, and return a cell population that looks nothing like what was in the animal. The biology is already hard — the sample prep shouldn't be the bottleneck.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"standard-markdown grid-cols-1 grid [\u0026amp;_\u0026gt;_*]:min-w-0 gap-3\"\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003eThis collection covers four dissociation kits built specifically for neural and sensory tissue, each formulated around the structural and biochemical properties of that tissue type.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"standard-markdown grid-cols-1 grid [\u0026amp;_\u0026gt;_*]:min-w-0 gap-3\"\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003ca href=\"https:\/\/firegene.com\/products\/mouse-brain-dissociation-kit-fg-ba3305?_pos=1\u0026amp;_sid=f65976421\u0026amp;_ss=r\"\u003e\u003cstrong\u003eMouse Brain Dissociation Kit (FG-BA3305)\u003c\/strong\u003e\u003c\/a\u003e Enzymatic formulation optimized for mouse neural tissue, where high lipid content and fragile neuronal populations make standard protocols unreliable. Used in scRNA-seq workflows requiring intact neurons, astrocytes, microglia, and oligodendrocytes without ex vivo transcriptional artifacts from over-digestion.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"standard-markdown grid-cols-1 grid [\u0026amp;_\u0026gt;_*]:min-w-0 gap-3\"\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003ca href=\"https:\/\/firegene.com\/products\/human-brain-dissociation-kit-fg-ba3326?_pos=1\u0026amp;_sid=d4a60e16e\u0026amp;_ss=r\"\u003e\u003cstrong\u003eHuman Brain Dissociation Kit (FG-BA3326)\u003c\/strong\u003e\u003c\/a\u003e Purpose-built for human brain tissue, which has a denser extracellular matrix and different myelination profile than rodent brain. Mouse brain protocols applied to human tissue typically yield lower viability and higher aggregation — this kit addresses both through species-specific enzyme calibration.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"standard-markdown grid-cols-1 grid [\u0026amp;_\u0026gt;_*]:min-w-0 gap-3\"\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003cstrong\u003eSpinal Cord Dissociation Kit\u003c\/strong\u003e Optimized for the connective tissue density and mixed cell composition of spinal cord — neurons, oligodendrocytes, astrocytes, microglia, and vascular cells in a structure that resists generic CNS protocols. Suited for neuroinflammation, ALS, spinal injury, and demyelinating disease research.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"standard-markdown grid-cols-1 grid [\u0026amp;_\u0026gt;_*]:min-w-0 gap-3\"\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003ca href=\"https:\/\/firegene.com\/products\/cochlea-dissociation-kit-fg-ba3321?_pos=1\u0026amp;_sid=bcec52eac\u0026amp;_ss=r\"\u003e\u003cstrong\u003eCochlea Dissociation Kit (FG-BA3321)\u003c\/strong\u003e\u003c\/a\u003e Designed for inner ear tissue isolation, where fragile hair cells and supporting cell populations are lost under standard enzymatic conditions. Enables single-cell characterization of cochlear cell types for hearing research and auditory neuroscience.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"standard-markdown grid-cols-1 grid [\u0026amp;_\u0026gt;_*]:min-w-0 gap-3\"\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003eAll four kits are validated for 10x Genomics Chromium, BD Rhapsody, and Drop-seq workflows.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","products":[{"product_id":"mouse-brain-dissociation-kit-fg-ba3305","title":"FireGene Mouse Brain Dissociation Kit for scRNA-seq","description":"\u003ch3 data-start=\"277\" data-end=\"291\" class=\"\"\u003eOverview\u003c\/h3\u003e\n\u003cp data-start=\"292\" data-end=\"598\" class=\"\"\u003eThe \u003cstrong data-start=\"296\" data-end=\"337\"\u003eFireGene Mouse Brain Dissociation Kit\u003c\/strong\u003e is engineered to produce high-quality single-cell suspensions from mouse brain tissue using an optimized enzymatic process. This kit is ideal for neuroscience research, enabling precise gene expression profiling and functional studies at the single-cell level.\u003c\/p\u003e\n\u003chr data-start=\"600\" data-end=\"603\" class=\"\"\u003e\n\u003ch3 data-start=\"605\" data-end=\"631\" class=\"\"\u003eBackground Information\u003c\/h3\u003e\n\u003cul data-start=\"633\" data-end=\"1647\"\u003e\n\u003cli data-start=\"633\" data-end=\"1242\" class=\"\"\u003e\n\u003cp data-start=\"635\" data-end=\"688\" class=\"\"\u003e\u003cstrong data-start=\"635\" data-end=\"688\"\u003eDriven by Clinical and Scientific Research Needs:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul data-start=\"691\" data-end=\"1242\"\u003e\n\u003cli data-start=\"691\" data-end=\"781\" class=\"\"\u003e\n\u003cp data-start=\"693\" data-end=\"781\" class=\"\"\u003eIncreased demand for high-resolution studies of \u003cstrong data-start=\"741\" data-end=\"780\"\u003ecellular heterogeneity in the brain\u003c\/strong\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"784\" data-end=\"885\" class=\"\"\u003eTraditional methods fail to preserve delicate brain cell populations and often yield low viability.\u003c\/li\u003e\n\u003cli data-start=\"888\" data-end=\"1145\" class=\"\"\u003e\n\u003cp data-start=\"890\" data-end=\"943\" class=\"\"\u003eSingle-cell sequencing of mouse brain tissue enables:\u003c\/p\u003e\n\u003cul data-start=\"948\" data-end=\"1145\"\u003e\n\u003cli data-start=\"948\" data-end=\"984\" class=\"\"\u003e\n\u003cp data-start=\"950\" data-end=\"984\" class=\"\"\u003eDetailed gene expression analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"989\" data-end=\"1066\" class=\"\"\u003e\n\u003cp data-start=\"991\" data-end=\"1066\" class=\"\"\u003eFunctional characterization of \u003cstrong data-start=\"1022\" data-end=\"1065\"\u003eneurons, glia, and other CNS cell types\u003c\/strong\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1071\" data-end=\"1145\" class=\"\"\u003e\n\u003cp data-start=\"1073\" data-end=\"1145\" class=\"\"\u003eDiscovery of new biomarkers and therapeutic targets for brain disorders.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1148\" data-end=\"1242\" class=\"\"\u003e\n\u003cp data-start=\"1150\" data-end=\"1242\" class=\"\"\u003eCritical for exploring \u003cstrong data-start=\"1173\" data-end=\"1230\"\u003eneurodevelopment, neurodegeneration, and brain repair\u003c\/strong\u003e mechanisms.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1244\" data-end=\"1647\" class=\"\"\u003e\n\u003cp data-start=\"1246\" data-end=\"1290\" class=\"\"\u003e\u003cstrong data-start=\"1246\" data-end=\"1290\"\u003eBackground of Technological Development:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul data-start=\"1293\" data-end=\"1647\"\u003e\n\u003cli data-start=\"1293\" data-end=\"1365\" class=\"\"\u003e\n\u003cp data-start=\"1295\" data-end=\"1365\" class=\"\"\u003eOvercomes limitations of mechanical and chemical dissociation methods.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1368\" data-end=\"1572\" class=\"\"\u003e\n\u003cp data-start=\"1370\" data-end=\"1416\" class=\"\"\u003eEmploys \u003cstrong data-start=\"1378\" data-end=\"1410\"\u003eadvanced enzymatic protocols\u003c\/strong\u003e that:\u003c\/p\u003e\n\u003cul data-start=\"1421\" data-end=\"1572\"\u003e\n\u003cli data-start=\"1421\" data-end=\"1480\" class=\"\"\u003e\n\u003cp data-start=\"1423\" data-end=\"1480\" class=\"\"\u003eUse optimized enzyme concentrations and incubation times.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1485\" data-end=\"1527\" class=\"\"\u003e\n\u003cp data-start=\"1487\" data-end=\"1527\" class=\"\"\u003eMinimize damage to fragile neural cells.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1532\" data-end=\"1572\" class=\"\"\u003e\n\u003cp data-start=\"1534\" data-end=\"1572\" class=\"\"\u003ePreserve cell viability and diversity.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1575\" data-end=\"1647\" class=\"\"\u003e\n\u003cp data-start=\"1577\" data-end=\"1647\" class=\"\"\u003eDesigned to ensure \u003cstrong data-start=\"1596\" data-end=\"1646\"\u003econsistent, reproducible dissociation outcomes\u003c\/strong\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"1649\" data-end=\"1652\" class=\"\"\u003e\n\u003ch3 data-start=\"1654\" data-end=\"1677\" class=\"\"\u003eDetection Principle\u003c\/h3\u003e\n\u003cul data-start=\"1679\" data-end=\"2240\"\u003e\n\u003cli data-start=\"1679\" data-end=\"1762\" class=\"\"\u003e\n\u003cp data-start=\"1681\" data-end=\"1762\" class=\"\"\u003eUtilizes a \u003cstrong data-start=\"1692\" data-end=\"1734\"\u003esynergistic enzymatic digestion method\u003c\/strong\u003e optimized for brain tissue.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1763\" data-end=\"2107\" class=\"\"\u003e\n\u003cp data-start=\"1765\" data-end=\"1784\" class=\"\"\u003eProcess highlights:\u003c\/p\u003e\n\u003cul data-start=\"1787\" data-end=\"2107\"\u003e\n\u003cli data-start=\"1787\" data-end=\"1858\" class=\"\"\u003e\n\u003cp data-start=\"1789\" data-end=\"1858\" class=\"\"\u003eMouse brain tissue is \u003cstrong data-start=\"1811\" data-end=\"1836\"\u003ecut into small pieces\u003c\/strong\u003e for uniform exposure.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1861\" data-end=\"1942\" class=\"\"\u003e\n\u003cp data-start=\"1863\" data-end=\"1942\" class=\"\"\u003e\u003cstrong data-start=\"1863\" data-end=\"1905\"\u003eSequential addition of enzyme mixtures\u003c\/strong\u003e facilitates gentle tissue breakdown.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1945\" data-end=\"2026\" class=\"\"\u003e\n\u003cp data-start=\"1947\" data-end=\"2026\" class=\"\"\u003eIncubation is performed under \u003cstrong data-start=\"1977\" data-end=\"2025\"\u003econtrolled temperature and timing conditions\u003c\/strong\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2029\" data-end=\"2107\" class=\"\"\u003e\n\u003cp data-start=\"2031\" data-end=\"2107\" class=\"\"\u003eEnzymes selectively degrade the \u003cstrong data-start=\"2063\" data-end=\"2106\"\u003eextracellular matrix and cell junctions\u003c\/strong\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2108\" data-end=\"2240\" class=\"\"\u003e\n\u003cp data-start=\"2110\" data-end=\"2123\" class=\"\"\u003eFinal output:\u003c\/p\u003e\n\u003cul data-start=\"2126\" data-end=\"2240\"\u003e\n\u003cli data-start=\"2126\" data-end=\"2240\" class=\"\"\u003e\n\u003cp data-start=\"2128\" data-end=\"2240\" class=\"\"\u003eA \u003cstrong data-start=\"2130\" data-end=\"2178\"\u003eclean, high-viability single-cell suspension\u003c\/strong\u003e ready for scRNA-seq, flow cytometry, and downstream analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 data-start=\"1654\" data-end=\"1677\" class=\"\"\u003eSpecifications\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eSingle-cell sequencing, cell culture or other cell-related detections\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eCompatible Sample Types\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eMouse brain tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eSupported Instruments\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eWater bath, horizontal centrifuge, cell counter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e-20 °C \/ 4 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eShelf-life\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e24 months at -20 °C\u003cbr\u003e12 months at 4 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 data-start=\"1654\" data-end=\"1677\" class=\"\"\u003eKit Components\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e10 reactions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"width: 25%; padding: 8px; border: 1px solid #ddd; background: #f0f0f0;\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"width: 25%; padding: 8px; border: 1px solid #ddd; background: #f0f0f0;\"\u003e10 Tests\/Kit\u003c\/th\u003e\n\u003cth style=\"width: 25%; padding: 8px; border: 1px solid #ddd; background: #f0f0f0;\"\u003eStorage\u003c\/th\u003e\n\u003cth style=\"width: 25%; padding: 8px; border: 1px solid #ddd; background: #f0f0f0;\"\u003eShelf-life\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003eBuffer A\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e1*21.6 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e-20 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003eEnzyme B\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e1*8 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e-20 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003eEnzyme C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e1*100 μL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e4 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003eDRS\u003cspan\u003e(Mouse Brain Cell Debris Removal Buffer)\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e1*10 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e4 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e50 reactions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"width: 25%; padding: 8px; border: 1px solid #ddd; background: #f0f0f0;\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"width: 25%; padding: 8px; border: 1px solid #ddd; background: #f0f0f0;\"\u003e10 Tests\/Kit\u003c\/th\u003e\n\u003cth style=\"width: 25%; padding: 8px; border: 1px solid #ddd; background: #f0f0f0;\"\u003eStorage\u003c\/th\u003e\n\u003cth style=\"width: 25%; padding: 8px; border: 1px solid #ddd; background: #f0f0f0;\"\u003eShelf-life\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003eBuffer A\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e5*21.6 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e-20 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003eEnzyme B\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e5*8 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e-20 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003eEnzyme C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e5*100 μL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e4 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003eDRS\u003cspan\u003e(Mouse Brain Cell Debris Removal Buffer)\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e5*10 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e4 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch4\u003e\u003c\/h4\u003e\n\u003ch4\u003eProduct Q\u0026amp;A\u003c\/h4\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e1. Q: Is this kit only suitable for mouse brain tissue? Is it applicable to brain tissue of other animals such as rats and rabbits? Can it be used for non-brain tissues of mice (e.g., liver, kidney)?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eA: This kit is specially designed for mouse brain tissue and is only suitable for the dissociation of mouse brain tissue. It is not recommended for use with brain tissue of other animals like rats and rabbits for the time being. The cell density and extracellular matrix composition of brain tissue vary among different animals, and the enzyme ratio and buffer components in the kit are optimized for mouse brain tissue. Using it on other animals may result in low dissociation efficiency or poor cell viability. Meanwhile, it cannot be used for non-brain tissues of mice. Non-brain tissues (such as liver and kidney) have significantly different structures from brain tissue, so a dedicated dissociation kit for the corresponding tissue (e.g., \u003ca href=\"https:\/\/firegene.com\/products\/liver-dissociation-kit-fg-ba3323?_pos=1\u0026amp;_sid=f8e64b327\u0026amp;_ss=r\"\u003eFG-BA3323 Liver Tissue Dissociation Kit\u003c\/a\u003e) should be used.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e2. Q: The components of the kit have different storage conditions. What should be noted before mixing and using them? If Enzyme C is accidentally stored frozen at -20°C, can it still be used?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: Two points should be noted before mixing and using: First, confirm that all components are within their validity period and stored in compliance with the required conditions (Buffer A and Enzyme B at -20°C; Enzyme C and DRS at 4°C in the dark). Second, before use, take Buffer A and Enzyme B out of the -20°C freezer, thaw them at room temperature, and mix thoroughly. Enzyme C and DRS can be directly taken out of the 4°C refrigerator and mixed well, with repeated freezing and thawing avoided. If Enzyme C is accidentally stored frozen at -20°C, it cannot be used anymore. Enzyme C is an enzyme preparation, and low-temperature freezing will destroy its spatial structure, leading to complete loss of enzyme activity. Using it will fail to dissociate the tissue effectively, and you need to contact the manufacturer to purchase a replacement.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e3. Q: Step 1 requires cutting about 200mg of fresh tissue. What impact will insufficient tissue quantity (e.g., only 50mg) or excessive tissue quantity (e.g., 300mg) have on the dissociation effect? How to adjust the operation?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: Insufficient tissue quantity (50mg) will lead to a relative excess of enzyme reagents, which may cause over-digestion of cells and reduce cell viability. Excessive tissue quantity (300mg) will result in insufficient enzyme reagents, which cannot fully break down the extracellular matrix, leading to incomplete tissue dissociation, a large number of tissue clumps, and low single-cell yield. Adjustment methods: When the tissue quantity is insufficient, the dosage of each reagent can be reduced proportionally (e.g., Buffer A reduced to 540μL, Enzyme B to 200μL, Enzyme C to 2.5μL) to ensure the ratio of enzyme to tissue is appropriate. When the tissue quantity is excessive, it should be divided into two portions for processing, and each portion should be operated with the reagent dosage corresponding to 200mg of tissue to avoid insufficient reagents affecting dissociation.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e4. Q: In Step 3, the digestion time is 20-30 minutes, and quality inspection is required every 3-5 minutes. What is the specific operation of quality inspection? How to judge whether digestion needs to be stopped?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: Specific operation of quality inspection: Take 10μL of cell suspension, add 10μL of AOPI or trypan blue staining solution (self-prepared), mix well, drop it onto a cell counting plate, and observe under a cell counter or microscope. There are two criteria for judging whether to stop digestion: First, cell viability. If the viability is low, digestion must be stopped even if the tissue is not fully dissociated to avoid over-digestion. Second, cell dispersion. When the proportion of tissue clumps in the field of view is ≤5% and the proportion of single cells is ≥90%, digestion can be stopped. If there are still many tissue clumps after 30 minutes but the cell viability is ≥70%, digestion can be extended for 5 minutes, but quality inspection must be conducted again to avoid exceeding 35 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e5. Q: Both Step 5 and Step 6 require filtration with a 70μm cell sieve. Why does Step 6 require rinsing the original centrifuge tube and filtering again? What consequences will occur if the rinsing and filtering in Step 6 are omitted?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: The purpose of rinsing and filtering in Step 6 is to recover the cells remaining on the wall of the original centrifuge tube. Digested cells may adhere to the inner wall of the centrifuge tube, and filtering only in Step 5 will cause the loss of these cells, reducing the single-cell yield. If this step is omitted, the cell yield may decrease by 15%-20%. Especially for samples with a small initial tissue quantity, it will seriously affect subsequent experiments (e.g., single-cell sequencing requires a sufficient number of cells). Therefore, the tube wall must be rinsed with 3mL of RPMI 1640 medium or PBS containing 5% FBS, and the rinsing solution must be filtered through the same 70μm cell sieve to ensure full cell recovery.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e  6. Q: After adding DRS in Step 11, it is necessary to gently pipette 10 times with a 5mL pipette, and \"vortex oscillation is not allowed\". What problems will vortex oscillation cause? What impacts will insufficient or excessive pipetting times have?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: Vortex oscillation will generate strong mechanical force, which damages the cell membrane of mouse brain cells, leading to cell rupture. At the same time, it will cause uneven mixing of DRS and cell suspension, affecting the subsequent density gradient stratification effect. Insufficient pipetting times (less than 10 times) will result in insufficient mixing of DRS and cell suspension, making it impossible to form stable debris layers and cell layers after centrifugation, leading to incomplete debris removal. Excessive pipetting times (more than 15 times) will damage cells due to mechanical friction, increase the proportion of dead cells, and may disrupt the density system of DRS, also affecting stratification. It is necessary to strictly control the pipetting to 10 times, with gentle movements to avoid generating air bubbles.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e 7. Q: Step 12 requires slowly adding 3mL of PBS along the tube wall to \"gently cover the top layer, and never mix\". If mixing occurs accidentally during addition, how to handle it? Is it necessary to re-operate?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: If mixing occurs accidentally when adding PBS, it will destroy the density gradient foundation formed by DRS and cell suspension. After centrifugation, it will be impossible to clearly separate the debris layer, supernatant, and cell pellet, resulting in the failure of debris removal. Therefore, re-operation is mandatory. For re-operation, centrifuge the mixed solution at 300×g for 5 minutes at 4°C, discard the supernatant, resuspend the pellet with 2mL of PBS containing 5% FBS, then add 1mL of DRS and gently pipette 10 times according to Step 11. After that, slowly add 3mL of PBS along the tube wall again, ensuring no mixing to avoid re-occurrence of errors.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e 8. Q: Steps 13 and 14 emphasize \"a horizontal centrifuge must be used\" and \"the centrifuge tube must be handled gently\". What impact will using a vertical centrifuge or handling the tube roughly after centrifugation have on stratification?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: When a vertical centrifuge is used, the direction of centrifugal force is perpendicular to the centrifuge tube, making it impossible for the solution to be evenly stratified according to the density gradient. This will cause debris, cells, and PBS to mix, and no clear three-layer structure can be separated. Rough handling of the centrifuge tube after centrifugation will cause violent shaking of the solution inside the tube, and the formed three-layer structure (supernatant, debris layer, cell pellet) will mix with each other. Debris will re-mix into the cell pellet, resulting in the loss of debris removal effect. Therefore, a horizontal centrifuge must be used, and after centrifugation, the centrifuge tube should be slowly taken out with both hands supporting the bottom to avoid any violent shaking.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e 9. Q: When adding \u003ca href=\"red%20blood%20cell%20lysis%20buffer%20(FG-BA3311)\"\u003ered blood cell lysis buffer (FG-BA3311)\u003c\/a\u003e in Step 15, what volume is the \"three times the volume\" calculated based on? Can the red blood cell lysis time on ice (5 minutes) be shortened or extended?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: \"Three times the volume\" is calculated based on the \"appropriate volume of PBS containing 5% FBS\" in Step 15. For example, if 100μL of PBS containing 5% FBS is used to resuspend the pellet, 300μL of red blood cell lysis buffer should be added. The red blood cell lysis time on ice cannot be shortened or extended: Shortening it to less than 5 minutes will result in incomplete red blood cell lysis, and residual red blood cells will mix into the brain cell suspension, interfering with subsequent cell counting and experiments (e.g., non-specific signals of red blood cells in flow cytometry analysis). Extending it to more than 5 minutes will make the lysis buffer toxic to brain cells, leading to decreased brain cell viability, especially for sensitive neuron cells. Thus, the time must be strictly controlled at 5 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e 10. Q: After quality control in Step 21, it is required to \"carry out subsequent experiments immediately\". If subsequent experiments cannot be conducted immediately, can the prepared brain cell suspension be stored for a short period? What are the restrictions on storage conditions and time?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: Short-term storage is possible, but there are strict restrictions on storage conditions and time: The cell suspension needs to be adjusted to a concentration of 1×10⁶-1×10⁷ cells\/mL (using PBS containing 5% FBS), placed in a sterile low-adhesion centrifuge tube, sealed, and stored in a 4°C refrigerator. The storage time should not exceed 1 hour, and repeated shaking should be avoided during storage. If stored for more than 1 hour, the viability of brain cells will decrease significantly (may be lower than 60%), and a small amount of new cell debris will appear. If stored for more than 2 hours, the cells will basically lose their viability and cannot be used for subsequent experiments (e.g., cell culture, single-cell sequencing). Before use, trypan blue staining must be re-conducted for quality inspection, and only cells with viability ≥70% can be used.\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"2 reactions\/kit","offer_id":47833138856148,"sku":"FG-BA3305-2rxns","price":69.0,"currency_code":"USD","in_stock":true},{"title":"10 reactions\/kit","offer_id":46299523645652,"sku":"FG-BA3305-10rxns","price":289.0,"currency_code":"USD","in_stock":true},{"title":"50 reactions\/kit","offer_id":47682162524372,"sku":"FG-BA3305-50rxns","price":1329.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/files\/BA3305.png?v=1773991058"},{"product_id":"cochlea-dissociation-kit-fg-ba3321","title":"FireGene Cochlea Dissociation Kit - Inner Ear Cell Prep","description":"\u003ch3 id=\"overview\"\u003eOverview\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Cochlea Dissociation Kit\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eis a specialized enzymatic solution optimized to isolate single cells from delicate cochlear tissue. Designed for use in single-cell sequencing and auditory research, this kit ensures high-viability cell suspensions for advanced studies in hearing loss, balance disorders, and neurobiology.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch3 id=\"background-information\"\u003eBackground Information\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eDriven by Clinical and Scientific Research Needs:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eUnderstanding inner ear biology and hearing mechanisms requires accurate dissociation of cochlear tissue into single cells.\u003c\/li\u003e\n\u003cli\u003eTraditional methods often fail to isolate\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ehair cells and auditory neurons\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003ewithout damage.\u003c\/li\u003e\n\u003cli\u003eThe kit supports:\n\u003cul\u003e\n\u003cli\u003eResearch on\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eage-related hearing loss, Ménière’s disease\u003c\/strong\u003e, and genetic hearing impairments.\u003c\/li\u003e\n\u003cli\u003eIdentification of gene expression profiles linked to\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ehair cell degeneration and regeneration\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eDevelopment of\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003etargeted therapies\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003efor restoring hearing and treating\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ebalance dysfunction\u003c\/strong\u003e.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBackground of Technological Development:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eMechanical or chemical dissociation methods often compromise cell integrity and yield.\u003c\/li\u003e\n\u003cli\u003eThis kit employs:\n\u003cul\u003e\n\u003cli\u003eA\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eprecise enzymatic digestion strategy\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003ethat matches the biochemical makeup of cochlear ECM.\u003c\/li\u003e\n\u003cli\u003eCarefully tuned\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eenzyme types, concentrations, and reaction times\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eA robust protocol to achieve\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eefficient, reproducible cell release\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003ewith excellent viability.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch3 id=\"detection-principle\"\u003eDetection Principle\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eBased on a\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003esynergistic enzymatic digestion process\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003etailored to the cochlea:\n\u003cul\u003e\n\u003cli\u003eCochlear tissue is\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003efinely sectioned\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003efor maximal enzyme exposure.\u003c\/li\u003e\n\u003cli\u003eReagents are added in sequence under\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003econtrolled temperature and timing\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eThe enzymes act to\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003edegrade ECM components and loosen intercellular adhesions\u003c\/strong\u003e.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003eFinal Output:\n\u003cul\u003e\n\u003cli\u003eA\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eclean, viable single-cell suspension\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eideal for scRNA-seq, auditory cell profiling, and regenerative studies.\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 id=\"detection-principle\"\u003eSpecifications\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eSingle-cell sequencing, cell culture or other cell-related detections\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eCompatible Sample Types\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eCochlea tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eSupported Instruments\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eWater bath, horizontal centrifuge, cell counter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e-20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eShelf-life\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 id=\"detection-principle\"\u003eKit Components\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"color: rgb(0, 0, 0); background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e10 Reactions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100.179%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40.0716%; font-weight: bold; padding: 8px; border: 1px solid rgb(221, 221, 221);\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 59.9284%; font-weight: bold; padding: 8px; border: 1px solid rgb(221, 221, 221);\"\u003e10 Reactions\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 40.0716%;\"\u003e\u003cspan\u003eCochlea DS (Cochlea Dissociation Solution)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 59.9284%;\"\u003e2 × 1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"color: rgb(0, 0, 0); background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e50 Reactions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 60%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003e50 Reactions\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e\u003cspan\u003eCochlea DS (Cochlea Dissociation Solution)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e10 × 1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eProduct FAQ\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e1.    Q: Is this kit only suitable for fresh cochlear tissue? Is it effective for cryopreserved and resuscitated cochlear tissue?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The kit is mainly suitable for fresh mammalian cochlear tissue (e.g., cochlear tissue from mice, rats, and humans). It should be used with caution for cryopreserved and resuscitated cochlear tissue: Sensitive cells in the cochlea (such as hair cells and supporting cells) are prone to rupture due to ice crystal damage during cryopreservation. After resuscitation, the viability of dissociated cells will be 25%-35% lower than that of fresh tissue. Pre-quality inspection via trypan blue staining is required (viability ≥55% to be usable).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e2.    Q: The instruction manual requires cutting 200mg of fresh cochlear tissue. Will insufficient tissue quantity (e.g., 80mg) or excessive tissue quantity (e.g., 250mg) affect the dissociation effect? How to adjust the operation?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Yes, it will affect the effect. ① Insufficient tissue quantity (\u0026lt;150mg): The content of target cells (e.g., hair cells) in cochlear tissue is inherently low. Insufficient quantity will result in an extremely low final single-cell yield (less than 40% of the normal amount), and the cell pellet will be unobvious during subsequent centrifugation and stratification, easily leading to collection omission. It is recommended to combine multiple 80mg cochlear tissue samples (to a total weight of 180-200mg) and add the dissociation solution according to the conventional dosage to ensure the total number of target cells meets experimental requirements. ② Excessive tissue quantity (\u0026gt;220mg): The space in a 5mL centrifuge tube is limited, so the tissue cannot be fully minced and dispersed. The enzymolysis solution cannot act evenly on all cells, leading to incomplete dissociation of local tissue and over-digestion of local cells. The tissue should be split into two tubes for processing, with each tube containing 180-200mg of tissue and corresponding to 240μL of dissociation solution + 2760μL of RPMI 1640 medium, to avoid the impact of tissue crowding on enzymolysis efficiency.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e3.    Q: Cochlear tissue contains sensitive cells such as hair cells, and the digestion time in Step 3 is 0.5-2 hours. How to determine the optimal digestion time? What impact will improper digestion time have on sensitive cells?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The optimal digestion time needs to be dynamically determined through \"microscopic quality inspection\", and observation is recommended every 15 minutes: ① When more than 80% of the cells in the field of view are in a single dispersed state, there are no obvious clusters of more than 5 cells, and trypan blue staining shows viability ≥70%, terminate digestion immediately. ② If hair cells (columnar in shape with obvious cilia) are observed to start swelling or rupturing, digestion must be terminated even if the minimum digestion time (0.5 hours) has not been reached. Insufficient digestion time: There are many tissue debris, and target cells such as hair cells are still wrapped in the tissue, resulting in a low single-cell yield. Excessive digestion time: Sensitive cells (such as hair cells and spiral ganglion cells) will have their cell membranes ruptured due to over-enzymolysis, leading to a sharp drop in viability. The proportion of target cells in subsequent experiments (e.g., single-cell sequencing) will be less than 30%, which cannot meet research needs.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e4.    Q: Steps 5 and 6 require filtration with a 70μm cell sieve and rinsing the centrifuge tube 3 times. What impact will omitting one rinsing step or replacing with a 50μm\/100μm sieve have on the cochlear cell suspension?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Omitting one rinsing step will cause approximately 1\/3 of the target cochlear cells (e.g., hair cells, supporting cells) to remain on the inner wall of the centrifuge tube, reducing the final yield by more than 30%. Cochlear cells are inherently small in quantity and easily adsorbed on the tube wall, so 3 rinsing steps are crucial to ensure full cell recovery. A 50μm or 100μm sieve cannot be used as a replacement: ① The 50μm sieve has too small a pore size. Although it can filter tissue debris, it will retain some smaller cochlear cells (e.g., spiral ganglion cells, with a diameter of approximately 8-12μm), leading to the loss of target cells. ② The 100μm sieve has too large a pore size and cannot effectively filter undigested cochlear tissue debris (e.g., basement membrane debris). These debris will enter subsequent steps along with the cells, interfering with the accuracy of cell counting and potentially clogging the microchannels of the single-cell sequencing chip, affecting the experimental process.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e5.    Q: The instruction manual mentions that \"DMEM medium can replace RPMI 1640 medium\". After replacement, is it necessary to adjust centrifugation parameters or digestion time? Is there any difference in the impact of the two media on the viability of sensitive cochlear cells (e.g., hair cells)?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: There is no need to adjust centrifugation parameters (still 4℃, 300×g for 5 minutes) or digestion time after replacement. Both DMEM and RPMI 1640 are common basal media for mammalian cells. Although they differ in glucose and amino acid content, both can provide a suitable osmotic pressure (280-320mOsm\/kg) and pH (7.2-7.4) for cochlear tissue dissociation, with no impact on enzymolysis efficiency. The impact on the viability of sensitive cochlear cells is minimal: Experimental data shows that after dissociating mouse cochlear tissue with the two media, the difference in hair cell viability is ≤6%, and the difference in the viability of supporting cells and nerve cells is ≤5%. The medium can be selected based on the existing inventory in the laboratory. If cochlear cell culture is required in subsequent steps, it is recommended to prioritize the medium consistent with the culture system to reduce the stress response of cells due to environmental changes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e6.    Q: The cochlear tissue dissociation solution needs to be stored at -20℃. If the ice pack melts during transportation and the reagent is left at 4℃ for 2 hours, can it still be used? What impact does repeated freezing and thawing have on the enzyme activity in the dissociation solution?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The reagent can still be used after being left at 4℃ for 2 hours, but it must be immediately returned to -20℃ and fully mixed before use. The enzymes (e.g., collagenase, hyaluronidase) in the dissociation solution lose ≤10% of their activity when stored at 4℃ for a short period (≤2 hours), and can still effectively destroy the extracellular matrix of cochlear tissue without affecting the dissociation effect. Repeated freezing and thawing will lead to a significant decrease in enzyme activity: Each freeze-thaw cycle reduces enzyme activity by 12%-15%; after more than 3 freeze-thaw cycles, the activity is less than 50%, which cannot fully decompose the fiber components in cochlear tissue. After dissociation, there will still be a large amount of tissue debris, and the release of target cells (e.g., hair cells) will decrease by more than 60%, which cannot meet experimental needs. It is recommended that after receiving the kit, aliquot the 2×1.25mL dissociation solution into 250μL\/tube (each tube corresponds to one experimental dose), seal it, store at -20℃, and take one tube for each experiment to avoid repeated freezing and thawing.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e7.    Q: The centrifugation parameters for Steps 8 and 9 are \"4℃, 300×g for 5 minutes\". If only a vertical centrifuge is available in the laboratory or the centrifugation speed is incorrect (e.g., 200×g, 400×g), what impact will this have on cochlear cells? Can room-temperature centrifugation replace 4℃ centrifugation?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: A vertical centrifuge cannot replace a horizontal centrifuge. The direction of centrifugal force of a vertical centrifuge is perpendicular to the centrifuge tube, which will cause uneven precipitation of cochlear cells, easily resulting in loose precipitation or adhesion to the tube wall. Sensitive cells (e.g., hair cells) are prone to being sucked away when discarding the supernatant; a horizontal centrifuge allows cells to precipitate evenly at the bottom of the tube, facilitating accurate collection. Impacts of incorrect parameters: ① Speed of 200×g: Insufficient centrifugal force causes cochlear cells (especially hair cells) to fail to precipitate fully and be lost with the supernatant, reducing the yield by more than 45%. ② Speed of 400×g: Excessive centrifugal force squeezes fragile hair cells and nerve cells, leading to cell membrane rupture and a 30%-35% decrease in viability. Room-temperature centrifugation cannot replace 4℃ centrifugation. Room temperature accelerates the metabolism of cochlear cells, and the residual enzymolysis solution has enhanced activity at room temperature, further damaging sensitive cells. Centrifugation at 4℃ can maintain a low metabolic state of cells, reduce viability loss, and ensure the quality of target cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e8.    Q: After quality control, it is required to \"carry out subsequent experiments immediately\". If subsequent experiments cannot be conducted immediately, can the prepared cochlear single-cell suspension be stored for a short period? What are the strict restrictions on storage conditions and time?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Short-term storage is possible, but the conditions are extremely strict: It must be stored in a sealed container at 4℃ in the dark for no more than 30 minutes, and repeated shaking or vibration must be avoided throughout the process. During storage, adjust the cell concentration to 1×10⁶-1×10⁷ cells\/mL using PBS containing 5% FBS, and place it in a low-adhesion centrifuge tube to prevent cell adhesion or damage from mechanical force. After 30 minutes, the viability of sensitive cochlear cells (e.g., hair cells) will decrease rapidly (15%-20% every 30 minutes), and cell aggregation is prone to occur; if stored for more than 1 hour, the viability of hair cells may be lower than 50%, making it unsuitable for high-precision experiments such as single-cell sequencing and cell function detection. Before use, re-conduct trypan blue staining quality inspection; only cells with viability ≥65% and single-cell ratio ≥80% can be used. At the same time, gently pipette 3-5 times (avoid violent pipetting to damage hair cell cilia) to disperse slightly aggregated cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e9.    Q: Compared with the \u003ca href=\"https:\/\/firegene.com\/products\/multi-tissue-dissociation-kit-fg-ba3303?_pos=2\u0026amp;_sid=0c1766ce1\u0026amp;_ss=r\"\u003emulti tissue dissociation kit of this brand (FG-BA3303)\u003c\/a\u003e, what are the core advantages of this cochlear-specific kit? Can \u003ca href=\"https:\/\/firegene.com\/products\/multi-tissue-dissociation-kit-fg-ba3303?_pos=2\u0026amp;_sid=0c1766ce1\u0026amp;_ss=r\"\u003eFG-BA3303\u003c\/a\u003e be used as a replacement for this product to dissociate cochlear tissue?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The core advantages lie in the \"optimization of the enzymolysis system for the characteristics of cochlear tissue\": ① Lower enzyme concentration (the enzyme concentration of FG-BA3321 dissociation solution is only 60% of that of \u003ca href=\"https:\/\/firegene.com\/products\/multi-tissue-dissociation-kit-fg-ba3303?_pos=2\u0026amp;_sid=0c1766ce1\u0026amp;_ss=r\"\u003eFG-BA3303\u003c\/a\u003e), avoiding over-digestion and damage to sensitive cells. ② Addition of hair cell protective components (e.g., low-concentration antioxidants) can reduce oxidative stress damage to hair cells during dissociation, and the hair cell recovery rate is more than 40% higher than that of \u003ca href=\"https:\/\/firegene.com\/products\/multi-tissue-dissociation-kit-fg-ba3303?_pos=2\u0026amp;_sid=0c1766ce1\u0026amp;_ss=r\"\u003eFG-BA3303\u003c\/a\u003e. ③ Better adaptability to enzymolysis time, which can balance tissue dissociation efficiency and cell viability without significant adjustments. \u003ca href=\"https:\/\/firegene.com\/products\/multi-tissue-dissociation-kit-fg-ba3303?_pos=2\u0026amp;_sid=0c1766ce1\u0026amp;_ss=r\"\u003eFG-BA3303\u003c\/a\u003e cannot be used as a replacement. \u003ca href=\"https:\/\/firegene.com\/products\/multi-tissue-dissociation-kit-fg-ba3303?_pos=2\u0026amp;_sid=0c1766ce1\u0026amp;_ss=r\"\u003eFG-BA3303\u003c\/a\u003e is a general-purpose kit, and its enzyme concentration and components are designed for ordinary tissues. When used for cochlear tissue, it is prone to massive death of hair cells and spiral ganglion cells, with viability less than 30%, and incomplete tissue dissociation, making it impossible to obtain a single-cell suspension that meets experimental needs. The cochlear-specific FG-BA3321 kit must be used.\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"2 reactions\/kit","offer_id":47833420759252,"sku":"FG-BA3321-2rxns","price":149.0,"currency_code":"USD","in_stock":true},{"title":"10 reactions\/kit","offer_id":46299523809492,"sku":"FG-BA3321-10rxns","price":589.0,"currency_code":"USD","in_stock":true},{"title":"50 reactions\/kit","offer_id":47707479441620,"sku":"FG-BA3321-50rxns","price":1949.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/files\/BA3321.png?v=1774247199"},{"product_id":"human-brain-dissociation-kit-fg-ba3326","title":"FireGene Human Brain Dissociation Kit for Neuroscience Research","description":"\u003ch3 id=\"overview\"\u003eOverview\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Human Brain Dissociation Kit\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eis a high-efficiency enzymatic system designed to dissociate human brain tissue into high-viability single-cell suspensions. Ideal for neurological research and single-cell sequencing, this kit enables accurate profiling of brain cell populations involved in health and disease.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch3 id=\"background-information\"\u003eBackground Information\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eDriven by Clinical and Scientific Research Needs:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSingle-cell sequencing of human brain tissue is essential for understanding\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ecomplex neural circuits\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eand\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ebrain disorders\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eTraditional dissociation methods struggle with preserving diverse brain cell types such as neurons and glia.\u003c\/li\u003e\n\u003cli\u003eThis kit enables:\n\u003cul\u003e\n\u003cli\u003eHigh-resolution analysis of\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ecellular heterogeneity\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003ein both healthy and diseased brain tissues.\u003c\/li\u003e\n\u003cli\u003eIdentification of\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ebiomarkers and therapeutic targets\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003efor conditions like\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eneurodegeneration, epilepsy\u003c\/strong\u003e, and\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003emental health disorders\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eAdvancement of\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eprecision neurology\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003ethrough better cellular insight.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBackground of Technological Development:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eOlder dissociation methods often lead to low cell yields and viability.\u003c\/li\u003e\n\u003cli\u003eFireGene’s solution:\n\u003cul\u003e\n\u003cli\u003eUses\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ea synergistic enzymatic blend\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eoptimized for fragile neural tissue.\u003c\/li\u003e\n\u003cli\u003eFeatures carefully calibrated\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eenzyme types, concentrations, and temperature conditions\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eMaximizes viable cell recovery for\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ereliable, reproducible results\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003ein downstream assays.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch3 id=\"detection-principle\"\u003eDetection Principle\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eUtilizes a\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003estepwise enzymatic digestion method\u003c\/strong\u003e:\n\u003cul\u003e\n\u003cli\u003eBrain samples are\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ecut into small pieces\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eto enhance enzyme penetration.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEnzymes are added sequentially\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eunder optimal incubation conditions.\u003c\/li\u003e\n\u003cli\u003eExtracellular matrix and intercellular junctions are enzymatically broken down.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003eResult:\n\u003cul\u003e\n\u003cli\u003eA\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eclean, viable single-cell suspension\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003esuitable for scRNA-seq, brain atlas mapping, flow cytometry, or culture.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 id=\"detection-principle\"\u003eSpecifications\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eSingle-cell sequencing, cell culture or other cell-related detections\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eCompatible Sample Types\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eHuman brain tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eSupported Instruments\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eWater bath, horizontal centrifuge, cell counter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e-20 °C \/ 4 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eShelf-life\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e24 months at -20 °C\u003cbr\u003e12 months at 4 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 id=\"detection-principle\"\u003eKit Components\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e10 Reactions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"width: 25%; padding: 8px; border: 1px solid #ddd; background: #f0f0f0;\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"width: 25%; padding: 8px; border: 1px solid #ddd; background: #f0f0f0;\"\u003e10 Reactions\/Kit\u003c\/th\u003e\n\u003cth style=\"width: 25%; padding: 8px; border: 1px solid #ddd; background: #f0f0f0;\"\u003eStorage\u003c\/th\u003e\n\u003cth style=\"width: 25%; padding: 8px; border: 1px solid #ddd; background: #f0f0f0;\"\u003eShelf-life\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003eBuffer A\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e1*21.6 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e-20 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003eEnzyme B\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e1*8 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e-20 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003eEnzyme C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e1*100 μL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e4 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e\u003cspan\u003eDRS (Human Brain Cell Debris Removal Buffer)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e1*10 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e4 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e50 Reactions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"width: 25.0447%; padding: 8px; border: 1px solid rgb(221, 221, 221); background: rgb(240, 240, 240);\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"width: 25.0447%; padding: 8px; border: 1px solid rgb(221, 221, 221); background: rgb(240, 240, 240);\"\u003e50 Reactions\/Kit\u003c\/th\u003e\n\u003cth style=\"width: 25.0447%; padding: 8px; border: 1px solid rgb(221, 221, 221); background: rgb(240, 240, 240);\"\u003eStorage\u003c\/th\u003e\n\u003cth style=\"width: 25.0447%; padding: 8px; border: 1px solid rgb(221, 221, 221); background: rgb(240, 240, 240);\"\u003eShelf-life\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 25.0447%;\"\u003eBuffer A\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 25.0447%;\"\u003e5*21.6 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 25.0447%;\"\u003e-20 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 25.0447%;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 25.0447%;\"\u003eEnzyme B\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 25.0447%;\"\u003e5*8 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 25.0447%;\"\u003e-20 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 25.0447%;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 25.0447%;\"\u003eEnzyme C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 25.0447%;\"\u003e5*100 μL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 25.0447%;\"\u003e4 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 25.0447%;\"\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 25.0447%;\"\u003e\u003cspan\u003eDRS (Human Brain Cell Debris Removal Buffer)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 25.0447%;\"\u003e5*10 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 25.0447%;\"\u003e4 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 25.0447%;\"\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eProduct FAQ\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e1.    Q: When dissociating human brain tissue, flocculent precipitates appear immediately after adding Enzyme C (10μL\/reaction). Will this affect the enzymolysis effect? How to handle the precipitation issue?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Flocculent precipitates are mostly caused by a temporary reaction between Enzyme C (enzyme preparation) and ionic components in Buffer A. If the precipitates dissolve on their own within 10 minutes, the enzymolysis effect will not be affected. If the precipitates persist, reconfigure the reaction system: ① First, mix 10μL Enzyme C with 800μL Enzyme B uniformly to form an \"enzyme mixture\"; ② Slowly add the mixture dropwise into Buffer A containing the tissue, and invert to mix while adding. This avoids precipitation caused by local high concentration and ensures stable enzymolysis efficiency.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e2.    Q: After adding DRS (Debris Removal Solution) in Step 11, the cell suspension is pipetted 10 times. What impact will excessive pipetting force or insufficient pipetting times have on cell separation? How to define the standard for \"gentle pipetting\"?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Excessive pipetting force: Damages the cell membrane of fragile cells such as neurons and glial cells, reducing cell viability by 30%-40%, with a large number of cell debris visible under the microscope. Insufficient pipetting times (\u0026lt;8 times): DRS cannot fully contact the cell suspension, so debris cannot float up effectively during subsequent centrifugation and stratification, reducing cell purity by 50%. Standard for \"gentle pipetting\": Use a 5mL pipette, insert the pipette tip slowly 1cm below the liquid surface when aspirating, control the aspiration time to 2 seconds, and push the liquid out slowly along the tube wall when dispensing. Avoid generating air bubbles and ensure stable, non-impact liquid flow.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e3.    Q: The centrifugation parameters in Step 13 are \"4℃, 3000×g, 20 minutes, medium acceleration\/deceleration\". If the laboratory's horizontal centrifuge does not have the \"medium acceleration\/deceleration\" function and only has \"fast\" or \"slow\" options, how to adjust the operation?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: If medium acceleration\/deceleration is unavailable, prioritize \"slow acceleration\/deceleration\" (acceleration time ≥5 minutes, deceleration time ≥8 minutes) and extend the centrifugation time to 25 minutes: ① Slow acceleration prevents liquid disturbance due to sudden increase in centrifugal force, ensuring stable stratification of DRS and cells; ② Slow deceleration prevents bottom cells from being scattered by inertia, reducing mixing with the debris layer. If only fast acceleration\/deceleration is available, let the centrifuge tube stand for 2-5 minutes after centrifugation before taking it out to stabilize the stratification interface. This reduces the probability of cell-debris mixing by 30%, but the cell yield will still be approximately 15% lower than that with medium acceleration\/deceleration.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e4.    Q: When dissociating aged human brain tissue (refrigerated for more than 6 hours after sampling), the cell viability is only 30%, much lower than the 70% of fresh tissue. How to optimize the operation to improve the cell viability of aged tissue?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The low viability of aged tissue requires optimization from two aspects: \"reducing cell damage\" and \"enhancing cell protection\": ① Immediately soak the tissue in PBS containing 10% FBS (5% for regular use) after sampling, and transport it under refrigeration at 4℃ to avoid tissue dehydration; ② Reduce the amount of Enzyme B to 600μL (800μL for regular use) during enzymolysis, and shorten the enzymolysis time to 15-20 minutes to reduce enzyme-induced damage to fragile cells; ③ Replace regular PBS with PBS containing 1% BSA in the washing step to enhance cell membrane protection. These measures can increase the cell viability of aged tissue to over 50%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e5.    Q: After adding red blood cell lysis buffer (\u003ca name=\"OLE_LINK2\"\u003e\u003c\/a\u003e\u003ca href=\"https:\/\/firegene.com\/products\/red-blood-cell-lysis-kit-fg-ba3311?_pos=5\u0026amp;_sid=8111c3f2d\u0026amp;_ss=r\"\u003e\u003cu\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Times New Roman;\"\u003eFG-\u003c\/span\u003e\u003c\/span\u003e\u003c\/u\u003e\u003cu\u003e\u003cspan class=\"15\"\u003eBA3311\u003c\/span\u003e\u003c\/u\u003e\u003c\/a\u003e) in Step 15, the mixture is incubated on ice for 5 minutes. What impact will prolonged incubation (e.g., 10 minutes) or insufficient incubation (e.g., 2 minutes) have on human brain cells (e.g., neurons)?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Prolonged incubation: Red blood cell lysis buffer has mild toxicity to neurons; 10-minute incubation reduces neuron viability by 25%-30%, and glial cells tend to shrink. Insufficient incubation: Red blood cells are not fully lysed, with residual red blood cells accounting for over 20%, which interferes with cell capture in subsequent single-cell sequencing (non-specific adsorption to the sequencing chip). The optimal operation is to observe under a microscope every 2 minutes and terminate the incubation immediately when red blood cells become transparent (approximately 4-5 minutes). This balances the effect of red blood cell removal and cell viability protection.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e6.    Q: Step 20 mentions \"if obvious cell clumping occurs, filter with a 20μm cell sieve\". However, after filtration, a large number of neurons (with a diameter of approximately 8-12μm) are retained. What is the reason, and how to adjust the filtration operation?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Neurons are retained mostly because they form clumps \u0026gt;20μm by entangling with glial cells and debris, rather than single neurons being retained. Adjustment methods: ① Before filtration, gently pipette the cell suspension 15 times with a 1mL low-adhesion pipette tip (avoid violence) to disperse small clumps \u0026lt;20μm; ② Pre-wet the 20μm cell sieve with PBS containing 5% FBS to reduce cell adsorption to the sieve; ③ Gently push the liquid with a 5mL syringe during filtration to avoid clump compression caused by sieve clogging. These measures can reduce the neuron retention rate from 40% to below 10%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e7.    Q: After opening Buffer A in the kit, it is stored at 4℃ for 1 month. When used, slight turbidity is found in the solution. Is it still usable? How to quickly determine if the turbid Buffer A is invalid?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Slight turbidity may be caused by the low-temperature precipitation of components in Buffer A. Its validity needs to be verified first: ① Take 100μL of turbid Buffer A and heat it in a 37℃ water bath for 10 minutes. If the turbidity disappears, it indicates normal precipitation; the buffer can be used after shaking uniformly. ② If the turbidity persists after heating or flocculent precipitates appear, Buffer A is contaminated or deteriorated and cannot be used. To avoid turbidity after opening, aliquot Buffer A into 2mL\/tube (one tube for one experiment) after opening, store it frozen at -20℃, and thaw and mix well before each use. This extends the validity period to 3 months.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e8.    Q: A 70μm cell sieve is used for filtration in Steps 5 and 6. If the sieve is severely clogged, the filtration speed is extremely slow, and a large amount of white flocculent material (suspected to be nerve fibers) remains on the sieve, how to pretreat to reduce nerve fiber residue?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The white flocculent material is nerve fibers. A \"pre-digestion\" step needs to be added before enzymolysis: ① After mincing the tissue, soak it in 2160μL Buffer A for 10 minutes, and gently shake every 5 minutes; ② Add 50μL Enzyme B (1\/16 of the regular 800μL), incubate at 37℃ for 5 minutes to preliminarily degrade nerve fibers; ③ Add the remaining Enzyme B and Enzyme C according to the regular procedure. This reduces nerve fiber formation by 60%, avoids sieve clogging, and increases the filtration speed by 40%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e9.    Q: After quality control, it is found that glial cells account for 90% of the cell suspension, while neurons only account for 10%—much lower than the normal neuron proportion in brain tissue (approximately 30%). What causes neuron loss, and how to optimize the collection method?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Neuron loss is mostly due to \"weak neuron sedimentation capacity\" in centrifugation and filtration steps: ① In Step 7, increase the centrifugation speed from 300×g to 350×g and extend the centrifugation time to 8 minutes to enhance neuron precipitation; ② Before filtration in Step 20, if there is no obvious cell clumping, skip the 20μm cell sieve filtration (neurons are easily adsorbed by the sieve); ③ When aspirating the supernatant in Step 14, retain 100μL of supernatant to mix with the cell pellet to avoid the loss of small-volume neurons with the supernatant. These measures can increase the neuron proportion to approximately 25%.\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"2 reactions\/kit","offer_id":47833516474580,"sku":"FG-BA3326-2rxns","price":109.0,"currency_code":"USD","in_stock":false},{"title":"10 reactions\/kit","offer_id":46299524432084,"sku":"FG-BA3326-10rxns","price":429.0,"currency_code":"USD","in_stock":true},{"title":"50 reactions\/kit","offer_id":47708600697044,"sku":"FG-BA3326-50rxns","price":1419.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/files\/BA3326.png?v=1774256387"},{"product_id":"spinal-cord-dissociation-kit","title":"FireGene Spinal Cord Dissociation Kit for Single-Cell Preparation","description":"\u003cp\u003e\u003cstrong\u003eFireGene Spinal Cord Dissociation Kit\u003c\/strong\u003e is designed for the isolation and preparation of single-cell suspensions from spinal cord tissue. The kit uses enzyme-based digestion to gently and rapidly disrupt extracellular matrix and release cells. The workflow includes digestion, filtration, washing, debris removal, optional red blood cell lysis, and quality control. Prepared cell suspensions can be used for single-cell sequencing, cell culture, and other cell-related assays.\u003c\/p\u003e\n\u003ch2\u003eBackground Information\u003c\/h2\u003e\n\u003cp\u003eSpinal cord tissue can contain fragile neural and glial cell populations along with debris generated during dissociation. A workflow that combines enzymatic digestion with debris removal helps improve sample clarity before downstream cellular analysis.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Spinal Cord Dissociation Kit for Single-Cell Preparation\u003c\/strong\u003e provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.\u003c\/p\u003e\n\u003ch3\u003eResearch Areas\u003c\/h3\u003e\n\u003cp\u003eThis product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eNeuroscience research\u003c\/li\u003e\n\u003cli\u003eSpinal cord injury models\u003c\/li\u003e\n\u003cli\u003eNeuroinflammation studies\u003c\/li\u003e\n\u003cli\u003eNeurodegeneration research\u003c\/li\u003e\n\u003cli\u003eGlial cell biology\u003c\/li\u003e\n\u003cli\u003eRegenerative medicine\u003c\/li\u003e\n\u003cli\u003eSingle-cell biology\u003c\/li\u003e\n\u003cli\u003eCell culture assay development\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKey Applications\u003c\/h3\u003e\n\u003cp\u003eThe prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSpinal cord single-cell suspension preparation\u003c\/li\u003e\n\u003cli\u003eSingle-cell sequencing\u003c\/li\u003e\n\u003cli\u003eFlow cytometry and cell sorting\u003c\/li\u003e\n\u003cli\u003ePrimary neural cell culture\u003c\/li\u003e\n\u003cli\u003eCell viability and counting\u003c\/li\u003e\n\u003cli\u003eDebris reduction after tissue dissociation\u003c\/li\u003e\n\u003cli\u003eCell-type composition analysis\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eSpinal Cord Dissociation Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBrand\u003c\/td\u003e\n\u003ctd\u003eFireGene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalog No.\u003c\/td\u003e\n\u003ctd\u003eFG-BA3334\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit Size\u003c\/td\u003e\n\u003ctd\u003e10 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Type\u003c\/td\u003e\n\u003ctd\u003eSpinal cord tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible Samples\u003c\/td\u003e\n\u003ctd\u003eFresh spinal cord tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended Starting Material\u003c\/td\u003e\n\u003ctd\u003eApproximately 200 mg tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Function\u003c\/td\u003e\n\u003ctd\u003ePreparation of spinal cord single-cell suspensions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWorkflow\u003c\/td\u003e\n\u003ctd\u003eEnzymatic digestion, filtration, centrifugation, debris removal, washing, optional RBC lysis, resuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDownstream Applications\u003c\/td\u003e\n\u003ctd\u003eSingle-cell sequencing, cell culture, cell-related assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDigestion Conditions\u003c\/td\u003e\n\u003ctd\u003e37°C for 20–30 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Instruments\u003c\/td\u003e\n\u003ctd\u003eWater bath or hybridization oven, horizontal centrifuge\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Reagents\u003c\/td\u003e\n\u003ctd\u003ePBS, FBS, RPMI 1640 medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Consumables\u003c\/td\u003e\n\u003ctd\u003eLow-adhesion pipette tips, centrifuge tubes, 20 μm and 70 μm cell strainers\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOptional Related Reagent\u003c\/td\u003e\n\u003ctd\u003eRed Blood Cell Lysis Solution, Catalog No. BA3311\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eBuffer A and Enzyme B: -20°C; Enzyme C and DRS: 4°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf Life\u003c\/td\u003e\n\u003ctd\u003eBuffer A and Enzyme B: 2 years; Enzyme C and DRS: 1 year\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eResearch Use\u003c\/td\u003e\n\u003ctd\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eCatalog Number\u003c\/th\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer A\u003c\/td\u003e\n\u003ctd\u003eFG-BA3334-A\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e21.6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEnzyme B\u003c\/td\u003e\n\u003ctd\u003eFG-BA3334-B\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e8 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEnzyme C\u003c\/td\u003e\n\u003ctd\u003eFG-BA3334-C\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e100 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDRS (Spinal Cord Cell Debris Removal Solution)\u003c\/td\u003e\n\u003ctd\u003eFG-BA3308-D\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"FireGene","offers":[{"title":"10 rxns","offer_id":47868495167700,"sku":"FG-BA3334-10","price":359.0,"currency_code":"USD","in_stock":true},{"title":"50 rxns","offer_id":47868495200468,"sku":"FG-BA3334-50","price":1119.0,"currency_code":"USD","in_stock":true}]}],"url":"https:\/\/firegene.com\/collections\/neuroscience-single-cell-sample-prep.oembed","provider":"FireGene","version":"1.0","type":"link"}