{"title":"Organoid Research","description":"\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003cstrong\u003eOrganoid Research\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003eOrganoids are only as good as the cells that seed them. Whether you're establishing intestinal crypts for a gut organoid line, isolating islets for pancreatic disease modeling, or characterizing gut epithelial cell populations by scRNA-seq, the dissociation step determines which cell types you actually recover — and how faithfully their transcriptomes reflect the tissue they came from.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003eThis collection covers three organ-specific dissociation kits used in organoid and single-cell workflows across GI biology, endocrinology, and model organism research.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003ca href=\"https:\/\/firegene.com\/products\/gastrointestinal-dissociation-kit-fg-ba3316?_pos=1\u0026amp;_sid=640ef7471\u0026amp;_ss=r\"\u003e\u003cstrong\u003eGastrointestinal Dissociation Kit (FG-BA3316)\u003c\/strong\u003e\u003c\/a\u003e Enzymatic dissociation kit optimized for viable single-cell suspensions from GI tissue, used in single-cell sequencing, immune profiling, and gut microbiota research. Suited for IBD, colorectal cancer, and intestinal epithelial biology applications where cell-type representation and viability from a structurally complex, mucus-rich tissue are the primary constraints. \u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003ca href=\"https:\/\/firegene.com\/products\/pancreas-dissociation-kit-fg-ba3314?_pos=1\u0026amp;_sid=2d803a8eb\u0026amp;_ss=r\"\u003e\u003cstrong\u003ePancreas Dissociation Kit (FG-BA3314)\u003c\/strong\u003e\u003c\/a\u003e Enzymatic kit for pancreatic tissue dissociation into viable single-cell suspensions, supporting investigations into diabetes, pancreatitis, and pancreatic cancer. The pancreas combines dense acinar exocrine tissue with rare endocrine islet populations — a composition that makes standard dissociation protocols unreliable for downstream single-cell analysis. \u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003ca href=\"https:\/\/firegene.com\/products\/moth-intestinal-dissociation-kit?_pos=1\u0026amp;_sid=7d302a51e\u0026amp;_ss=r\"\u003e\u003cstrong\u003eMoth Intestinal Dissociation Kit \u003c\/strong\u003e\u003c\/a\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/firegene.com\/products\/pancreas-dissociation-kit-fg-ba3314?_pos=1\u0026amp;_sid=2d803a8eb\u0026amp;_ss=r\"\u003e(FG-BA3347)\u003c\/a\u003e\u003c\/strong\u003e Single-cell preparation from lepidopteran intestinal tissue for insect gut biology, host-pathogen interaction studies, and comparative GI research using model organisms. One of few commercially available dissociation kits specifically formulated for insect intestinal tissue.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003eAll three kits are validated for 10x Genomics Chromium, BD Rhapsody, and Drop-seq workflows.\u003c\/p\u003e","products":[{"product_id":"pancreas-dissociation-kit-fg-ba3314","title":"FireGene Pancreas Dissociation Kit - High-Fidelity Enzymatic Digestion","description":"\u003ch3 id=\"overview\"\u003eOverview\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Pancreas Dissociation Kit\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eis designed for the efficient enzymatic dissociation of pancreatic tissue into viable single-cell suspensions. Ideal for single-cell sequencing and disease modeling, this kit supports investigations into diabetes, pancreatitis, and pancreatic cancer with high precision and reproducibility.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch3 id=\"background-information\"\u003eBackground Information\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eDriven by Clinical and Scientific Research Needs:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eUnderstanding pancreatic disease mechanisms, such as\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ediabetes, chronic inflammation\u003c\/strong\u003e, and\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003epancreatic cancer\u003c\/strong\u003e, requires detailed single-cell analysis.\u003c\/li\u003e\n\u003cli\u003eTraditional methods do not fully preserve\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eislet cells\u003c\/strong\u003e,\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eacinar cells\u003c\/strong\u003e, and other fragile populations.\u003c\/li\u003e\n\u003cli\u003eThis kit enables:\n\u003cul\u003e\n\u003cli\u003eIsolation and profiling of pancreatic cell types for\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ebiomarker discovery\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eImproved insight into\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eimmune response, fibrosis, and beta-cell function\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eDevelopment of\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003etargeted therapies\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eand regenerative strategies for pancreatic diseases.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBackground of Technological Development:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eFireGene overcomes the limitations of low cell yield and viability by using:\n\u003cul\u003e\n\u003cli\u003eA\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eproprietary enzymatic formulation\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003edesigned specifically for pancreatic ECM.\u003c\/li\u003e\n\u003cli\u003eOptimized conditions for\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eincubation time, temperature, and enzyme concentration\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eA workflow that preserves\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ecell integrity\u003c\/strong\u003e, ensuring consistent and high-quality single-cell suspensions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch3 id=\"detection-principle\"\u003eDetection Principle\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eBased on a\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003esynergistic enzymatic digestion process\u003c\/strong\u003e:\n\u003cul\u003e\n\u003cli\u003ePancreatic tissue is\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eminced and treated sequentially\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003ewith prepared reagents.\u003c\/li\u003e\n\u003cli\u003eThe digestion proceeds under\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003econtrolled conditions\u003c\/strong\u003e, breaking down ECM components and intercellular junctions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003eFinal Result:\n\u003cul\u003e\n\u003cli\u003eA\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eclean, high-viability single-cell suspension\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eideal for use in scRNA-seq, proteomics, and pancreatic disease research.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 id=\"detection-principle\"\u003eSpecifications\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eSingle-cell sequencing, cell culture or other cell-related detections\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eCompatible Sample Types\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003ePancreas tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eSupported Instruments\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eWater bath, horizontal centrifuge, cell counter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e-80 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eShelf-life\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 id=\"detection-principle\"\u003eKit Components\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40.0716%; font-weight: bold; padding: 8px; border: 1px solid rgb(221, 221, 221);\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 59.9284%; font-weight: bold; padding: 8px; border: 1px solid rgb(221, 221, 221);\"\u003e8 Tests\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 40.0716%;\"\u003e\n\u003cp\u003ePancreas Dissociation Kit\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 59.9284%;\"\u003e8 × 3 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eProduct FAQ\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003e1.   Q: Is this kit only suitable for mammalian pancreatic tissue? Are there differences in dissociation effects on different parts of the pancreas (e.g., islets, exocrine portion)? Can it be used for pancreatic tumor tissue or non-mammalian pancreatic tissue (e.g., avian pancreas)?\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The kit is only suitable for normal pancreatic tissue and pancreatic cancer tissue, and is not temporarily recommended for non-mammalian pancreatic tissue. The cell membrane characteristics of non-mammalian pancreatic tissue (such as avian pancreas) are significantly different from those of mammals, making it difficult to obtain intact single cells after dissociation. There are slight differences in dissociation effects on different parts of the pancreas: For islet tissue (containing sensitive endocrine cells), the digestion time needs to be shortened (it is recommended to complete quality inspection within 0.5 hours) to avoid reduced viability due to excessive enzymolysis; the exocrine portion has slightly stronger tolerance and can be digested for the conventional 0.5 hours. However, both can eventually obtain high-viability single cells with no significant difference in yield.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e2.    Q: The kit is labeled \"8 rxns\", and the dosage of pancreatic tissue dissociation solution per experiment is 3mL. If only 100mg of pancreatic tissue (1\/2 of the standard dosage) is processed in a single experiment, can the dosage of dissociation solution be proportionally reduced to 1.5mL?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Proportional reduction to 1.5mL is not recommended. The enzyme concentration and buffer system in the pancreatic tissue dissociation solution are designed for 3mL volume to match 200mg of tissue. If reduced to 1.5mL, the contact area between the enzyme and the tissue will be significantly reduced, and the excessively small liquid volume may lead to high local enzyme concentration. On the contrary, this will cause over-digestion of some tissues and incomplete dissociation of others. When processing 100mg of tissue, 3mL of dissociation solution is still required. During subsequent centrifugation to collect cells, the centrifugation time can be extended (from 5 minutes to 8 minutes) to ensure sufficient cell precipitation and avoid cell loss due to the relatively large liquid volume.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e3.    Q: During digestion in Step 3, a water bath requires \"manual shaking every 3-5 minutes\", and a hybridization oven requires \"a rotation speed of 20-30 rpm\". Is the only operational difference between the two devices the mixing method? Which one is more suitable for pancreatic tissue dissociation?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Beyond the mixing method, the core difference lies in temperature stability: The temperature control accuracy of the hybridization oven (±0.5℃) is higher than that of the water bath (±1℃). Moreover, the automatic rotation speed enables continuous and uniform contact between pancreatic tissue and the dissociation solution, avoiding local digestion differences caused by uneven density of pancreatic tissue. In terms of effect, the hybridization oven is more suitable for pancreatic tissue dissociation—Pancreatic tissue contains a large number of digestive enzymes. If the frequency or intensity of manual shaking is inconsistent, it is easy to cause \"local over-enzymolysis and local undigestion\"; the stable rotation speed of the hybridization oven can reduce this risk, and the single-cell yield is 15%-20% higher than that of the water bath. If a water bath is used, it is necessary to strictly shake at 3-minute intervals for 10 seconds each time to ensure no tissue settles at the bottom.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e4.    Q: Steps 5-7 require filtration with a 70μm cell sieve and rinsing the centrifuge tube 3 times to collect a total of 12mL of filtrate. What impact will omitting one rinsing step have on the experimental results? Can a 40μm or 100μm cell sieve replace the 70μm one?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Omitting one rinsing step will result in the loss of approximately 1\/3 of residual cells, reducing the final cell yield by 25%-30%. Cells after pancreatic dissociation (such as islet β cells and acinar cells) are easily adsorbed on the inner wall of the centrifuge tube, and 3 rinsing steps are the key to ensuring full cell recovery. A 40μm or 100μm cell sieve cannot be used as a replacement: The pore size of the 40μm sieve is too small and will retain some pancreatic single cells (e.g., islet cells have a diameter of about 10-15μm; although they can pass through, the sieve is easily blocked by fiber debris, leading to filtration difficulties); the pore size of the 100μm sieve is too large and cannot effectively filter the fine fiber debris remaining in pancreatic tissue. These impurities will interfere with subsequent cell counting or single-cell sequencing sample preparation.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e5.    Q: The centrifugation parameters in Steps 8 and 9 are both \"4℃, 300×g for 5 minutes\". If only a vertical centrifuge is available in the laboratory or the centrifugation speed is set incorrectly (e.g., 200×g, 400×g), what impact will this have? Can room-temperature centrifugation replace 4℃ centrifugation?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: A vertical centrifuge cannot replace a horizontal centrifuge. The direction of centrifugal force of a vertical centrifuge is perpendicular to the centrifuge tube, which will cause uneven precipitation of pancreatic cells, easily resulting in loose precipitation or adhesion to the tube wall. Cells are likely to be sucked away when discarding the supernatant; a horizontal centrifuge allows cells to precipitate evenly at the bottom of the tube, facilitating subsequent operations. Impacts of incorrect parameters: ① Rotation speed of 200×g: Cells cannot be fully precipitated and will be lost with the supernatant, reducing the yield by more than 40%; ② Rotation speed of 400×g: Excessive centrifugal force will squeeze fragile islet cells, leading to a 20%-25% decrease in viability. Room-temperature centrifugation cannot replace 4℃ centrifugation. Room temperature will accelerate the metabolism of pancreatic cells, and the residual dissociation solution has enhanced activity at room temperature, further damaging the cells. Centrifugation at 4℃ can maintain a low metabolic state of cells and reduce viability loss.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e6.    Q: The instruction manual mentions that \"DMEM medium can replace RPMI 1640 medium\". After replacement, is it necessary to adjust the number of rinsing steps or centrifugation parameters? Do the two media have an impact on the viability of pancreatic cells?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: There is no need to adjust the number of rinsing steps or centrifugation parameters after replacement. Both DMEM and RPMI 1640 are common basal media for mammalian cells. Although they differ in amino acid and vitamin content, both can provide a suitable osmotic pressure and pH environment for pancreatic cells, with no impact on dissociation efficiency. The impact on the viability of pancreatic cells is minimal: Experimental data shows that the difference in pancreatic cell viability after rinsing with the two media is ≤5%. The medium can be selected based on the existing inventory in the laboratory without deliberate replacement. If the subsequent experiment is cell culture, it is recommended to prioritize the medium consistent with the culture system to reduce the cell adaptation cost.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e7.    Q: Step 12 mentions that \"BA3311 Red Blood Cell Lysis Buffer can be used to remove red blood cells\". After which step should this operation be performed? What should be noted during lysis to avoid damaging pancreatic cells?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: It should be performed after Step 10 (after two washes) and before Step 11 (before resuspension). Specific operation: After discarding the supernatant in Step 10, add 1mL of BA3311 Red Blood Cell Lysis Buffer, incubate at 4℃ for 5 minutes (avoid room-temperature incubation to prevent damage to pancreatic cells), then centrifuge at 4℃, 300×g for 5 minutes, discard the supernatant, and resuspend with 5mL of PBS containing 5% FBS (add an additional wash), then proceed to Step 11. Precautions: The lysis time should not exceed 8 minutes; otherwise, the pancreatic cell membrane will be damaged. If there are many red blood cells, lysis can be repeated once, but an additional wash is required to avoid residual lysis buffer affecting subsequent experiments (e.g., cell capture efficiency in single-cell sequencing).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e8.    Q: The pancreatic tissue dissociation solution needs to be stored at -80℃. If the ice pack melts during transportation and the reagent is left at 4℃ for 2 hours, can it still be used? What impact does repeated freezing and thawing have?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: It can still be used after being left at 4℃ for 2 hours, but it must be immediately returned to -80℃ and fully mixed before subsequent use. The enzyme in the dissociation solution loses ≤10% of its activity when placed at 4℃ for a short period (≤2 hours), which does not affect the dissociation effect. Repeated freezing and thawing will lead to a significant decrease in enzyme activity: Each freeze-thaw cycle reduces enzyme activity by 12%-18%; after more than 3 freeze-thaw cycles, the activity is less than 50%, which cannot effectively decompose the extracellular matrix of pancreatic tissue, resulting in a large number of tissue clumps after dissociation. It is recommended that after receiving the kit, aliquot the 4×3mL dissociation solution into 3mL\/tube (no further aliquoting is needed, as the original packaging is already in single-use volume), seal it, store at -80℃, and take one tube for each experiment to avoid repeated freezing and thawing.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e9.    Q: After quality control in Step 13, it is required to \"carry out subsequent experiments immediately\". If subsequent experiments cannot be conducted immediately, can the prepared pancreatic cell suspension be stored for a short period? What are the restrictions on storage conditions and time?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Short-term storage is possible under the conditions of sealed storage in a 4℃ refrigerator for no more than 1.5 hours, with repeated shaking avoided. During storage, the cell concentration should be adjusted to 1×10⁶-1×10⁷ cells\/mL using PBS containing 5% FBS, and the suspension should be placed in a low-adhesion centrifuge tube. After 1.5 hours, the viability of pancreatic cells (especially islet cells) will decrease significantly (10%-12% per hour), and cell aggregation is prone to occur; if stored for more than 3 hours, the cell viability may be lower than 50%, making it unsuitable for single-cell sequencing or cell culture. Before use, re-quality inspection is required, and only cells with viability ≥65% can be used. Additionally, gently pipette 5-8 times to disperse slightly aggregated cells.\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"8 Reactions\/kit","offer_id":46299524497620,"sku":"FG-BA3314-8","price":889.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/files\/BA3314.png?v=1774421468"},{"product_id":"gastrointestinal-dissociation-kit-fg-ba3316","title":"FireGene Gastrointestinal Dissociation Kit - Gut Cell Isolation","description":"\u003ch3 id=\"overview\"\u003eOverview\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Gastrointestinal Dissociation Kit\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eis a high-performance enzymatic solution designed to isolate viable single-cell suspensions from GI tissues. Optimized for applications like single-cell sequencing, immune profiling, and gut microbiota research, this kit delivers exceptional results for complex and fragile gastrointestinal samples.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch3 id=\"background-information\"\u003eBackground Information\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eDriven by Clinical and Scientific Research Needs:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSingle-cell sequencing of GI tissue is critical for understanding diseases such as\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eIBD (inflammatory bowel disease)\u003c\/strong\u003e,\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ecolorectal cancer\u003c\/strong\u003e, and\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eintestinal fibrosis\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eConventional methods do not fully preserve the\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ediversity of epithelial, stromal, and immune cells\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eThis kit enables:\n\u003cul\u003e\n\u003cli\u003eDetailed characterization of\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ecell subsets involved in inflammation, cancer, and tissue repair\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eIdentification of biomarkers and therapeutic targets for\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eprecision medicine\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eSupport for regenerative and microbiome-associated GI research.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBackground of Technological Development:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eTraditional techniques often result in\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003elow cell viability and inefficient dissociation\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eFireGene resolves these limitations by:\n\u003cul\u003e\n\u003cli\u003eUsing a\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003etargeted enzymatic system\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003etailored for gut ECM and cell junctions.\u003c\/li\u003e\n\u003cli\u003eOptimizing\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ereaction conditions\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003efor reproducibility across different GI tissue types.\u003c\/li\u003e\n\u003cli\u003eDelivering high cell recovery rates suitable for\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003esensitive downstream applications\u003c\/strong\u003e.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch3 id=\"detection-principle\"\u003eDetection Principle\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eUses a\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003emulti-step enzymatic digestion approach\u003c\/strong\u003e:\n\u003cul\u003e\n\u003cli\u003eGI tissue is\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ecut into small fragments\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003efor uniform reagent exposure.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEnzymes are applied sequentially\u003c\/strong\u003e, breaking down extracellular matrix and cellular adhesions.\u003c\/li\u003e\n\u003cli\u003eThe process occurs under\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ecarefully regulated incubation conditions\u003c\/strong\u003e.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003eOutcome:\n\u003cul\u003e\n\u003cli\u003eA\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ehigh-quality, viable single-cell suspension\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eideal for scRNA-seq, flow cytometry, and functional GI assays.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 id=\"detection-principle\"\u003eSpecifications\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eSingle-cell sequencing, cell culture or other cell-related detections\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eCompatible Sample Types\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eGastrointestinal tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eSupported Instruments\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eWater bath, horizontal centrifuge, cell counter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e-20 °C \/ 4 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eShelf-life\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e24 months at -20 °C\u003cbr\u003e12 months at 4 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 id=\"detection-principle\"\u003eKit Components\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e10 Reactions\/kit\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px; height: 78.3752px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003cth style=\"width: 25.0447%; padding: 8px; border: 1px solid rgb(221, 221, 221); background: rgb(240, 240, 240); height: 19.5938px;\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"width: 25.0447%; padding: 8px; border: 1px solid rgb(221, 221, 221); background: rgb(240, 240, 240); height: 19.5938px;\"\u003eQuantity\u003c\/th\u003e\n\u003cth style=\"width: 25.0447%; padding: 8px; border: 1px solid rgb(221, 221, 221); background: rgb(240, 240, 240); height: 19.5938px;\"\u003eStorage\u003c\/th\u003e\n\u003cth style=\"width: 25.0447%; padding: 8px; border: 1px solid rgb(221, 221, 221); background: rgb(240, 240, 240); height: 19.5938px;\"\u003eShelf-life\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003eBuffer A\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003e40 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003e-20 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003eEnzyme B\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003e1 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003e-20 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003eEnzyme C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003e100 μL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003e4 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e50 Reactions\/kit\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px; height: 78.3752px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003cth style=\"width: 25.0447%; padding: 8px; border: 1px solid rgb(221, 221, 221); background: rgb(240, 240, 240); height: 19.5938px;\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"width: 25.0447%; padding: 8px; border: 1px solid rgb(221, 221, 221); background: rgb(240, 240, 240); height: 19.5938px;\"\u003eQuantity\u003c\/th\u003e\n\u003cth style=\"width: 25.0447%; padding: 8px; border: 1px solid rgb(221, 221, 221); background: rgb(240, 240, 240); height: 19.5938px;\"\u003eStorage\u003c\/th\u003e\n\u003cth style=\"width: 24.8658%; padding: 8px; border: 1px solid rgb(221, 221, 221); background: rgb(240, 240, 240); height: 19.5938px;\"\u003eShelf-life\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003eBuffer A\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003e5*40 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003e-20 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 24.8658%;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003eEnzyme B\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003e5*1 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003e-20 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 24.8658%;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003eEnzyme C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003e5*100 μL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003e4 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 24.8658%;\"\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eProduct FAQ\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e1.    Q: Is this kit only suitable for mammalian gastrointestinal tissue? Are there differences in dissociation effects on tissues from different parts of the gastrointestinal tract (e.g., gastric mucosa, small intestinal villi, colonic mucosa)? Can it be used for non-mammalian gastrointestinal tissue (e.g., fish intestines)?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The kit is suitable for full-segment gastrointestinal tissue (stomach, small intestine, colon, etc.) of mammals such as humans and mice. There are slight differences in dissociation effects on tissues from different parts: small intestinal villi tissue is relatively fragile, so the digestion time is recommended to be shortened to 20-30 minutes to avoid villus structure breakage; gastric mucosa and colonic mucosa contain more collagen fibers, so the digestion time can be extended to 30-40 minutes, and dynamic adjustment is required through quality inspection. It is not suitable for non-mammalian gastrointestinal tissue. The cell membrane structure and fiber components of non-mammalian gastrointestinal cells (such as fish intestines) are significantly different from those of mammals. The enzyme system of the kit cannot accurately degrade their extracellular matrix, which easily leads to incomplete dissociation or a sharp drop in cell viability.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e2.    Q: The kit is labeled \"10 rxns\". Each experiment requires 4mL of Buffer A, 100μL of Enzyme B, and 10μL of Enzyme C. If only 100mg of gastrointestinal tissue (1\/2 of the standard dosage) is processed in a single experiment, can the reagent dosage be reduced proportionally?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Proportional reduction of reagent dosage is not recommended. Buffer A is not only a reaction medium but also needs to maintain the tissue suspension environment; a volume of 4mL can ensure the full dispersion of tissue fragments. The ratio of Enzyme B to Enzyme C is precisely formulated; reducing the dosage will lead to insufficient enzyme concentration, which cannot effectively decompose the fibers of gastrointestinal tissue and instead increases the cell clumping rate. When processing 100mg of tissue, reagents should still be added according to the standard dosage. During subsequent centrifugation to collect cells, the centrifugation time can be extended (from 5 minutes to 8 minutes) to ensure sufficient cell precipitation and avoid cell loss due to the relatively large liquid volume.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e3.    Q: During digestion in Step 2, a water bath requires \"manual shaking every 3-5 minutes\", and a hybridization oven requires \"a rotation speed of 20-30 rpm\". What are the core differences in operation effects between the two devices? Which one is more suitable for gastrointestinal tissue dissociation?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The core difference lies in \"the uniformity of enzyme-tissue contact\": the automatic rotation speed of the hybridization oven can keep gastrointestinal tissue fragments suspended in the dissociation solution at all times, avoiding local over-enzymolysis (e.g., tissue at the bottom of the tube) or insufficient enzymolysis (e.g., tissue at the liquid surface) caused by gravity sedimentation. If the manual shaking frequency of the water bath is inconsistent, it is easy to cause over-digestion of small intestinal villi tissue and incomplete dissociation of colonic mucosa tissue. In terms of effect, the hybridization oven is more suitable for gastrointestinal tissue dissociation, especially for fiber-rich tissues such as the colon, with a single-cell yield 18%-25% higher than that of the water bath. If a water bath is used, it is necessary to strictly shake at 3-minute intervals for 10 seconds each time to ensure no tissue settles at the bottom.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e4.    Q: Both Step 5 and Step 6 require filtration with a 70μm cell sieve. If the laboratory lacks a 70μm cell sieve, can it be replaced with a 50μm or 100μm cell sieve? What impact will the replacement have?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: A 50μm or 100μm cell sieve cannot be used as a replacement. The pore size of the 50μm sieve is too small and will retain normal single cells in gastrointestinal tissue (e.g., the diameter of small intestinal epithelial cells is about 12-18μm; although they can pass through, the sieve is easily blocked by fiber fragments, leading to filtration difficulties and even cell extrusion to reduce viability). The pore size of the 100μm sieve is too large and cannot effectively filter undigested tissue fragments (e.g., gastric mucosa fiber fragments). These fragments will enter subsequent steps along with cells, interfering with the accuracy of cell counting and may even block the single-cell sequencing chip. If a 70μm cell sieve is lacking, it is necessary to contact the supplier for emergency deployment or select a compatible 70μm cell sieve of the same brand (product number: BS70-C); random replacement is not allowed.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e5.    Q: The instruction manual mentions that \"DMEM medium can replace RPMI 1640 medium\". After replacement, is it necessary to adjust centrifugation parameters or digestion time? Is there any difference in the impact of the two media on the viability of gastrointestinal cells?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: There is no need to adjust centrifugation parameters (still 4℃, 300×g for 5 minutes) or digestion time after replacement. Both DMEM and RPMI 1640 are common basal media for mammalian cells. Although they differ in glucose and amino acid content, both can provide a suitable osmotic pressure (280-320mOsm\/kg) and pH (7.2-7.4) for gastrointestinal tissue dissociation, with no impact on enzymolysis efficiency. The impact on the viability of gastrointestinal cells is minimal: experimental data shows that the difference in cell viability after dissociating mouse small intestinal tissue with the two media is ≤6%. The medium can be selected based on the existing inventory in the laboratory without deliberate replacement.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e6.    Q: Step 7 uses PBS containing 5% FBS to wash cells. If serum-free operation is required for the experiment (e.g., some cell sorting experiments), can PBS without FBS be used as a replacement? Or can serum substitutes (e.g., BSA) replace FBS?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: PBS without FBS cannot be used as a replacement. The core function of FBS is to neutralize residual enzyme activity (Enzyme B and Enzyme C) to prevent enzymes from continuing to damage cells; PBS without FBS cannot neutralize enzyme activity, which will lead to a more than 30% drop in cell viability after washing. If serum-free operation is required, PBS containing 1% BSA can be used to replace PBS containing 5% FBS. BSA can play a similar enzyme-neutralizing role and does not contain serum components; however, it should be noted that after replacing with BSA, the washing time needs to be extended from 5 minutes to 8 minutes to ensure complete neutralization of enzyme activity and avoid residues affecting subsequent experiments.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e7.    Q: Enzyme C needs to be stored at 4℃ in the dark. If it is left at room temperature for 30 minutes (e.g., forgotten to be put back in the refrigerator after use), can it still be used? What consequences will occur if Enzyme C is stored at -20℃ for a long time?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: It can still be used after being left at room temperature for 30 minutes, but it must be immediately put back in the 4℃ refrigerator and fully mixed before use. Enzyme C is an active enzyme preparation; short-term storage at room temperature (≤30 minutes) results in ≤10% activity loss, which does not affect the dissociation effect. Long-term storage of Enzyme C at -20℃ is not allowed. Low temperature will cause Enzyme C to denature and inactivate (after 24 hours of freezing at -20℃, activity decreases by more than 70%), making it unable to work synergistically with Enzyme B to decompose gastrointestinal tissue fibers, leading to dissociation failure. The correct storage condition for Enzyme C is 4℃ in the dark, with a validity period of 1 year, which must be strictly followed.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e8.    Q: If there are many red blood cells in the cell suspension after dissociation and \u003ca href=\"https:\/\/firegene.com\/products\/red-blood-cell-lysis-kit-fg-ba3311?_pos=12\u0026amp;_sid=c5ff19011\u0026amp;_ss=r\"\u003eFG-BA3311 Red Blood Cell Lysis Buffer\u003c\/a\u003e is needed to remove them, after which step should this operation be performed? What should be noted during lysis to avoid damaging gastrointestinal cells?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: This operation should be performed after Step 7 and before Step 8. Specific operation: After discarding the supernatant in Step 7, add 1mL of FG-BA3311 Red Blood Cell Lysis Buffer, incubate on ice for 5 minutes (to avoid damaging gastrointestinal cells due to room-temperature incubation), centrifuge at 4℃, 300×g for 5 minutes, discard the supernatant, then resuspend with 5mL of PBS containing 5% FBS (add an additional washing step), and then proceed to Step 8. Precautions: ① The lysis time should not exceed 8 minutes; ice incubation can reduce the toxicity of the lysis buffer to gastrointestinal cells; ② If there are too many red blood cells (e.g., gastric mucosa tissue contains a large number of capillaries), lysis can be repeated once, but an additional PBS washing step is required to avoid residual lysis buffer affecting subsequent experiments (e.g., interference with fluorescence signals in cell sorting).\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"2 reactions\/kit","offer_id":47833362956500,"sku":"FG-BA3316-2rxns","price":189.0,"currency_code":"USD","in_stock":true},{"title":"10 reactions\/kit","offer_id":46299524563156,"sku":"FG-BA3316-10rxns","price":759.0,"currency_code":"USD","in_stock":true},{"title":"50 reactions\/kit","offer_id":47718733316308,"sku":"FG-BA3316-50rxns","price":2759.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/files\/BA3316_8da3ff52-d799-4f95-a195-65af984be99f.png?v=1774513519"},{"product_id":"moth-intestinal-dissociation-kit","title":"FireGene Moth Intestinal Dissociation Kit for Single-Cell Preparation","description":"\u003cp\u003e\u003cstrong\u003eFireGene Moth Intestinal Dissociation Kit\u003c\/strong\u003e is designed for the isolation and preparation of cell suspensions from moth intestinal tissues. The kit uses enzymatic digestion to gently and effectively break down extracellular matrix and release cells. Prepared cell suspensions can be used for cell sorting, cell culture, and other cell-related assays. The workflow is optimized for insect tissue handling, with gentle pipetting and wide-bore tips recommended because insect cells are relatively fragile.\u003c\/p\u003e\n\u003ch2\u003eBackground Information\u003c\/h2\u003e\n\u003cp\u003eMoth intestinal tissue is relevant to insect physiology, host-pathogen interaction, microbiome, and pest biology research. Because insect cells can be fragile, gentle processing conditions are important during dissociation and resuspension.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Moth Intestinal Dissociation Kit for Single-Cell Preparation\u003c\/strong\u003e provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.\u003c\/p\u003e\n\u003ch3\u003eResearch Areas\u003c\/h3\u003e\n\u003cp\u003eThis product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eInsect biology\u003c\/li\u003e\n\u003cli\u003eMoth intestinal physiology\u003c\/li\u003e\n\u003cli\u003eHost-pathogen interaction studies\u003c\/li\u003e\n\u003cli\u003eInsect microbiome research\u003c\/li\u003e\n\u003cli\u003ePest biology and toxicology\u003c\/li\u003e\n\u003cli\u003eCell culture assay development\u003c\/li\u003e\n\u003cli\u003eCell sorting workflows\u003c\/li\u003e\n\u003cli\u003eComparative cell biology\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKey Applications\u003c\/h3\u003e\n\u003cp\u003eThe prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eMoth intestinal cell suspension preparation\u003c\/li\u003e\n\u003cli\u003eCell sorting\u003c\/li\u003e\n\u003cli\u003eCell culture\u003c\/li\u003e\n\u003cli\u003eCell viability and counting\u003c\/li\u003e\n\u003cli\u003eInsect intestinal tissue analysis\u003c\/li\u003e\n\u003cli\u003eCell-based assays\u003c\/li\u003e\n\u003cli\u003eTissue-specific insect research\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eMoth Intestinal Dissociation Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBrand\u003c\/td\u003e\n\u003ctd\u003eFireGene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalog No.\u003c\/td\u003e\n\u003ctd\u003eFG-BA3347\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit Size\u003c\/td\u003e\n\u003ctd\u003e10 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Type\u003c\/td\u003e\n\u003ctd\u003eMoth intestinal tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible Samples\u003c\/td\u003e\n\u003ctd\u003eFresh intestinal tissues from moths\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended Starting Material\u003c\/td\u003e\n\u003ctd\u003eIntestinal tissues from 10–15 fresh moths\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Function\u003c\/td\u003e\n\u003ctd\u003ePreparation of moth intestinal cell suspensions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWorkflow\u003c\/td\u003e\n\u003ctd\u003eEnzymatic digestion, filtration, centrifugation, optional wash, resuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDownstream Applications\u003c\/td\u003e\n\u003ctd\u003eCell sorting, cell culture, cell-related assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDigestion Conditions\u003c\/td\u003e\n\u003ctd\u003e28°C for 25–35 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Instruments\u003c\/td\u003e\n\u003ctd\u003eWater bath or hybridization oven, horizontal centrifuge\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Reagents\u003c\/td\u003e\n\u003ctd\u003eD-Hanks buffer, calcium and magnesium free; FBS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Consumables\u003c\/td\u003e\n\u003ctd\u003eLow-adhesion pipette tips, 5 mL and 15 mL centrifuge tubes, 40 μm cell strainers\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf Life\u003c\/td\u003e\n\u003ctd\u003eTwo years\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eResearch Use\u003c\/td\u003e\n\u003ctd\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eCatalog Number\u003c\/th\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eMIDS (Moth Intestinal Dissociation Solution)\u003c\/td\u003e\n\u003ctd\u003eFG-BA3347-A\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"FireGene","offers":[{"title":"10 rxns","offer_id":47868495462612,"sku":"FG-BA3347-10","price":539.0,"currency_code":"USD","in_stock":true},{"title":"50 rxns","offer_id":47868495495380,"sku":"FG-BA3347-50","price":1769.0,"currency_code":"USD","in_stock":true}]}],"url":"https:\/\/firegene.com\/collections\/organoid-research.oembed","provider":"FireGene","version":"1.0","type":"link"}