{"title":"PCR qPCR and Isothermal Amplification Master Mixes","description":"","products":[{"product_id":"firegene-eia-basic-kit","title":"FireGene EIA Basic Kit","description":"\u003cp\u003eThe FireGene EIA Basic Kit is a basic isothermal amplification kit with DNA or cDNA as the template. It can be used for nucleic acid electrophoresis detection and has the advantages of high amplification yield, strong specificity and good stability. Combined with specific probes designed for the target gene, using enzymes such as EXO III or Nfo for specific recognition and hydrolysis of the probe, the target gene can be accurately quantified and detected, with good repeatability and high reliability. The minimum detection limit of this kit is 10~100 copies (according to different primer screening optimization procedures and detection methods).\u003c\/p\u003e\n\u003ch4\u003eApplications\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eNucleic acid electrophoresis detection\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/h4\u003e\n\u003ctable style=\"width: 100%;\" data-mce-style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 60%;\" data-mce-style=\"width: 60%;\"\u003e\u003cstrong\u003eComponent Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%;\" data-mce-style=\"width: 20%;\"\u003e\u003cstrong\u003eAmount\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%;\" data-mce-style=\"width: 20%;\"\u003e\u003cstrong\u003eStorage (°C)\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003eEIA Basic Enzyme Mix\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e530 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003eEIA Basic Reaction Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e1 ml\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003e\u003cspan\u003eActivator\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e200 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003e\u003cspan\u003eForward primer (F)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e24 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e-20\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003e\u003cspan\u003eReverse primer (R)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e24 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e-20\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003e\u003cspan\u003ePositive control\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e20 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e-20\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003e\u003cspan\u003e6 x DNA Loading Buffer\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e500 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e-20\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003eRestrictions\u003cbr\u003e\n\u003c\/h4\u003e\n\u003cp\u003e\u003cspan\u003eFor Research or Educational Use Only.\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42892686065876,"sku":"FG-M00101","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder_c94298bd-c0cb-4dc9-8599-0dc48b419e89.jpg?v=1656611343"},{"product_id":"firegen-one-step-rt-lamp-sybr-green-kit","title":"FireGene One Step RT-LAMP SYBR Green kit","description":"\u003cp\u003e\u003cspan data-mce-fragment=\"1\"\u003eThe FireGene One-Step RT-LAMP\u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003e SYBR Green kit is a reverse transcription loop-mediated isothermal amplification (RT-LAMP) based \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003ekit.\u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003e The RT-LAMP Enzyme Mix\u003c\/span\u003e contains the Bst DNA Polymerase and the M-MLV (H- ) Reverse Transcriptase (heat-resistant) as the key enzymes. It can be used for SYBR® Green I fluorescence quantification of LAMP with high sensitivity and strong specificity. Combined with the Buffer optimized for SYBR® Green I method, it can inhibit non-specific amplification, thereby significantly improving the amplification efficiency, and is suitable for high-sensitivity dye-based LAMP detection reactions. This product is provided in a convenient Mix, which can obtain a good standard curve in a wide range, and can accurately quantify and detect the target gene with high repeatability and reliability.\u003c\/p\u003e\n\u003ch4\u003eApplications\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eGene expression analysis of gene targets\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/h4\u003e\n\u003ctable style=\"width: 100%;\" data-mce-style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 60%;\" data-mce-style=\"width: 60%;\"\u003e\u003cstrong\u003eComponent Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%;\" data-mce-style=\"width: 20%;\"\u003e\u003cstrong\u003eAmount\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%;\" data-mce-style=\"width: 20%;\"\u003e\u003cstrong\u003eStorage (°C)\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003e\n\u003cspan\u003eRNase free ddH\u003c\/span\u003e\u003csub\u003e2\u003c\/sub\u003e\u003cspan\u003eO\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e1.25 ml x 4\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003e\n\u003cspan\u003e2 x LAMP SYBR Reaction Mix\u003c\/span\u003e\u003csup\u003ea\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e1.25 ml x 4\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003e\n\u003cspan\u003eRT LAMP Enzyme Mix\u003c\/span\u003e\u003csup\u003eb\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e125 μl x 4\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003ea. Contains dNTPs, Mg2+ and SYBR® Green I\u003c\/p\u003e\n\u003cp\u003eb. Contains Bst DNA Polymerase, M-MLV (H- ) Reverse Transcriptase (heat-resistant), RNasin, etc.\u003c\/p\u003e\n\u003ch4\u003eRestrictions\u003cbr\u003e\n\u003c\/h4\u003e\n\u003cp\u003e\u003cspan\u003eFor Research or Educational Use Only.\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv id=\"gtx-trans\" style=\"position: absolute; left: -54px; top: 566.223px;\"\u003e\n\u003cdiv class=\"gtx-trans-icon\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42895928525012,"sku":"FG-M00202","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder_58660dbf-b20d-4ccc-8425-47831360f76a.jpg?v=1656610396"},{"product_id":"magic-high-fidelity-2x-master-mix-for-ngs","title":"Magic High-Fidelity 2X Master Mix for NGS","description":"\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eMagic High-Fidelity DNA Polymerase is a novel B-family DNA polymerase, engineered to have increased affinity for DNA. The intrinsic high processivity of the enzyme results in significant improvement in yield, speed and sensitivity when compared with wild-type B-family DNA polymerases. In addition, the ability to amplify long targets, as well as GC- and AT-rich targets, is significantly improved. Magic High-Fidelity  DNA Polymerase has 5’\u0026amp;rightarrow;3’ polymerase and 3’\u0026amp;rightarrow;5’ exonuclease (proofreading) activity, but no 5’\u0026amp;rightarrow;3’ exonuclease activity. The strong 3’\u0026amp;rightarrow;5’ exonuclease activity results in superior accuracy during DNA amplification, lending to Magic High-Fidelity DNA Polymerase the lowest published error rate of all B-family DNA polymerases (1 error per 3.6 x 106 nucleotides incorporated). This fidelity is approximately 100 times higher than that of wild-type Taq DNA polymerase, and up to 10 times higher than that of other B-family DNA polymerases and polymerase blends.\n             \n             In Magic High-Fidelity  2X PCR Master Mix with HF Buffer, Magic High-Fidelity  DNA Polymerase is supplied in a convenient 2X Master Mix format, containing all reaction components except primers and template. The Master Mix contains Magic High-Fidelity DNA Polymerase (1 U per 50 µL reaction) in a proprietary reaction buffer containing dNTPs (0.3 mM of each dNTP at 1X), MgCl2 (1.5 mM at 1X) and stabilizers. Magic High-Fidelity 2X PCR Master Mix with HF Buffer is designed for routine, high-fidelity PCR of a wide range of targets and fragment sizes. In addition, Magic High-Fidelity  DNA polymerase requires significantly shorter reaction times than wild-type B-family DNA polymerases.\n             \n             DNA fragments generated with Magic High-Fidelity  2X PCR Master Mix may be used for routine downstream analysis and applications, including restriction enzyme digestion, cloning and sequencing. PCR products generated with Magic High-Fidelity  2X PCR Master Mix are blunt-ended, but may be 3’-dA-tailed for cloning into TA cloning vectors.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eHigh Fidelity PCR, High throughput PCR , Routine PCR\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e100 rxn \/ 500 rxn\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e2x\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eHigh-Fidelity 2X Master Mix for NGS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eFor Research Use Only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eStore the High-Fidelity 2X Master Mix for NGS at -20°C. Please avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42990251802836,"sku":"FG-ENY124","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder_755c85a1-e414-41d8-9c69-b0202fc889d7.jpg?v=1657059555"},{"product_id":"rnase-inhibitor-mammalian","title":"RNase Inhibitor, Mammalian","description":"\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eRNase Inhibitor, Mammalian specifically inhibits RNases A, B and C. It inhibits RNases by binding noncovalently in a 1:1 ratio with high affinity. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor is used with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3). The 50 kDa protein inhibits RNases by binding noncovalently in a 1:1 ratio with an association constant greater than 10^14.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eAn E.coli strain that carries the Ribonuclease Inhibitor gene from Porcine.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eCell-Free Protein Expression, PCR, RT-PCR,cDNA synthesis,In vitro transcription\/translation,Enzymatic RNA labeling reaction,Other applications where the integrity of RNA is important\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e2000 U \/ 10000 U \/ 20000 U\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e40,000 U\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eRNase Inhibitor, Mammalian (40,000 U\/ml)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eOne unit is defined as the amount of RNase Inhibitor, Mammalian required to inhibit the activity of 5ng of RNase A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2', 3'-cyclic monophosphate by RNase A.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eFor Research Use Only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eStore the RNase Inhibitor, Mammalian at -20°C. Please avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42990251868372,"sku":"FG-ENY126","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder_11491ea3-ce48-40fd-bc13-ba3a2847a649.jpg?v=1657059558"}],"url":"https:\/\/firegene.com\/collections\/pcr-qpcr-and-isothermal-amplification-master-mixes.oembed","provider":"FireGene","version":"1.0","type":"link"}