{"title":"PCR qPCR and Molecular Biology Reagents","description":"\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003cstrong\u003ePCR, qPCR \u0026amp; Molecular Biology Reagents\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003eThe enzymes at the center of your workflow don't get enough credit — until one of them fails.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003eThis collection covers the core reagents that molecular biology runs on, from the Taq polymerase in a routine genotyping gel to the high-fidelity enzyme feeding your NGS library prep. Twenty-eight products across six subcategories, organized below by where they sit in the workflow. \u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003cstrong\u003eDNA amplification\u003c\/strong\u003e Standard and hot-start Taq formulations for routine PCR; MagicFast Pfu and Magic High-Fidelity polymerases and 2× master mixes for applications where error rate matters — cloning inserts, long-range amplification, NGS library construction. Dye and no-dye master mix variants available.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003cstrong\u003eIsothermal amplification\u003c\/strong\u003e Bst DNA Polymerase (full length and large fragment) for LAMP-based assays. RT-LAMP SYBR Green kit for single-tube RNA detection without a thermocycler — useful for point-of-care workflow development and field diagnostics.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003cstrong\u003eReverse transcription and cDNA synthesis\u003c\/strong\u003e MagicScript II reverse transcriptase, first-strand cDNA synthesis kits, and RT master mixes for qPCR — including gDNA wiper variants that eliminate genomic background without a separate DNase step.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003cstrong\u003eCloning and mutagenesis\u003c\/strong\u003e One-Step Cloning Kit for seamless ligation-independent assembly; Fast Site-Directed Mutagenesis Kit built on a high-fidelity amplification and rapid recombination system for plasmids up to 20 kb.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003cstrong\u003eModifying enzymes and library prep tools\u003c\/strong\u003e T4 DNA Ligase (high concentration), T4 DNA Polymerase, Klenow Fragment, T4 Polynucleotide Kinase, UDG, RNase Inhibitor, and Tn5 Transposase — the accessory enzymes behind end repair, adapter ligation, tagmentation, and RNA protection steps in NGS and single-cell workflows.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003cstrong\u003eIn vitro transcription\u003c\/strong\u003e T7 RNA Polymerase for in vitro RNA synthesis.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003eShips worldwide. Bulk and institutional pricing available on request.\u003c\/p\u003e","products":[{"product_id":"bst-dna-polymerase-large-fragment","title":"Bst DNA Polymerase, Large Fragment","description":"\u003cp\u003eBst DNA Polymerase, Large Fragment is the portion of the Bacillus stearothermophilus DNA Polymerase protein that contains the 5' \u0026amp;rightarrow; 3' polymerase activity, but lacks 5' \u0026amp;rightarrow; 3' exonuclease activity. This polymerase also has strand displacement capabilities making it an ideal candidate for isothermal amplification.\u003c\/p\u003e\n\u003ch4\u003eApplication\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eIsothermal amplification (LAMP, RCA)\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003eDNA sequencing of high GC DNA\u003c\/li\u003e\n\u003cli\u003eSequencing from very low amounts of template DNA\u003c\/li\u003e\n\u003cli\u003eDNA strand displacement amplification\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003eUnit Definition\u003c\/h4\u003e\n\u003cp\u003eOne unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C.\u003c\/p\u003e\n\u003ch4\u003eComponents\u003c\/h4\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 60%;\"\u003e\u003cstrong\u003eComponent Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%;\"\u003e\u003cstrong\u003eAmount\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%;\"\u003e\u003cstrong\u003eStorage (°C)\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\"\u003eBst DNA Polymerase Large Fragment (8 U\/μl)\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\"\u003e1.25 ml\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\"\u003e\u003cspan\u003e10 x Bst Buffer\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\"\u003e\u003cspan\u003e2 x 1.25 ml\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003eRestrictions\u003c\/h4\u003e\n\u003cp\u003e\u003cspan\u003eFor Research Use Only\u003c\/span\u003e\u003c\/p\u003e\n\u003ch4\u003e\u003c\/h4\u003e\n\u003cdiv id=\"gtx-trans\" style=\"position: absolute; left: -31px; top: 311.625px;\"\u003e\n\u003cdiv class=\"gtx-trans-icon\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42892321947860,"sku":"FG-M00102","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder_74ee035d-162e-47be-ba25-aa3d54034ff0.jpg?v=1656610248"},{"product_id":"bst-dna-polymerase-full-length","title":"Bst DNA Polymerase, Full Length","description":"\u003cp\u003eBst DNA Polymerase, \u003cspan\u003eFull Length\u003c\/span\u003e is the full length p\u003cspan\u003eolymerase from\u003c\/span\u003e Bacillus stearothermophilus that contains the 5' \u0026amp;rightarrow; 3' polymerase activity and the 5' \u0026amp;rightarrow; 3' exonuclease activity. This polymerase also has strand displacement capabilities making it an ideal candidate for isothermal amplification.\u003c\/p\u003e\n\u003ch4\u003eApplications\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eIsothermal amplification (LAMP, RCA)\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003eDNA strand displacement amplification\u003c\/li\u003e\n\u003cli\u003ePrimer extension\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003e\u003cbr\u003e\u003c\/h4\u003e\n\u003ch4\u003e\u003cspan\u003eUnit Definition\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003e\u003cspan\u003eOne unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eStorage: \u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003eStore at -20°C for 2 years.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eEnzyme Concentration: \u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e8 U\u003c\/span\u003e\u003cspan\u003enits\u003c\/span\u003e\u003cspan\u003e\/μl\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch4\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/h4\u003e\n\u003ctable class=\"16\" border=\"1\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"162\" valign=\"top\"\u003e\n\u003cp class=\"15\" align=\"center\"\u003e\u003cspan\u003eComponent Name\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"135\" valign=\"top\"\u003e\n\u003cp class=\"15\" align=\"center\"\u003e\u003cspan\u003e100 Reactions\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"162\" valign=\"top\"\u003e\n\u003cp class=\"15\" align=\"center\"\u003e\u003cspan\u003eBst\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eII DNA Polymerase\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"135\" valign=\"top\"\u003e\n\u003cp class=\"15\" align=\"center\"\u003e\u003cspan\u003e200μl\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"162\" valign=\"top\"\u003e\n\u003cp class=\"15\" align=\"center\"\u003e\u003cspan\u003e5×LAMP Reaction Mix\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"135\" valign=\"top\"\u003e\n\u003cp class=\"15\" align=\"center\"\u003e\u003cspan\u003e0.6\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eml\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"162\" valign=\"top\"\u003e\n\u003cp class=\"15\" align=\"center\"\u003e\u003cspan\u003eTS\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eLAMP\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eGreen(20×)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"135\" valign=\"top\"\u003e\n\u003cp class=\"15\" align=\"center\"\u003e\u003cspan\u003e100μl\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"162\" valign=\"top\"\u003e\n\u003cp class=\"15\" align=\"center\"\u003e\u003cspan\u003e6×DNA Loading Buffer\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"135\" valign=\"top\"\u003e\n\u003cp class=\"15\" align=\"center\"\u003e\u003cspan\u003e500  μl\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"162\" valign=\"top\"\u003e\n\u003cp class=\"15\" align=\"center\"\u003e\u003cspan\u003eNuclease-free Water\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"135\" valign=\"top\"\u003e\n\u003cp class=\"15\" align=\"center\"\u003e\u003cspan\u003e2×1ml\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch4\u003eRestrictions\u003c\/h4\u003e\n\u003cp\u003e\u003cspan\u003eFor Research Use Only.\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42892399149268,"sku":"FG-LP301-100rxns","price":298.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder.jpg?v=1656610265"},{"product_id":"t7-rna-polymerase","title":"T7 RNA Polymerase","description":"\u003cp\u003eT7 RNA Polymerase is isolated from recombinant E.coli BL21 strain \u003cspan data-mce-fragment=\"1\"\u003ecarrying a plasmid which contains the T7 RNA Polymerase gene without\u003c\/span\u003e endogenous nucleic acid residues nor endonuclease residues. T7 RNA Polymerase \u003cspan data-mce-fragment=\"1\"\u003eexhibits extremely high specificity\u003c\/span\u003e to the T7 phage promoter \u003cspan data-mce-fragment=\"1\"\u003esequences\u003c\/span\u003e (TAATACGACT CACTATAG) and can be used as the promoter. \u003cspan data-mce-fragment=\"1\"\u003eT7 DNA or DNA cloned downstream from a T7 promoter can serve as a template for T7 RNA Polymerase-directed RNA synthesis.\u003c\/span\u003e The double-stranded linear blunt-end or the5' overhang DNA can be used as substrate template for T7 RNA Polymerase, hence, linear plasmids and PCR products can be used as templates for in vitro RNA synthesis.\u003c\/p\u003e\n\u003ch4\u003eApplications\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eGeneration of templates for in vitro translation.\u003c\/li\u003e\n\u003cli\u003eGeneration of probes for nucleic acid hybridizations.\u003c\/li\u003e\n\u003cli\u003eGeneration of RNA processing substrates.\u003c\/li\u003e\n\u003cli\u003eGeneration of antisense RNA.\u003c\/li\u003e\n\u003cli\u003emRNA, sgRNA synthesis\u003c\/li\u003e\n\u003cli\u003eRadiolabeled RNA probe preparation\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003e\u003cspan\u003eUnit Definition\u003c\/span\u003e\u003c\/h4\u003e\n\u003cp\u003e\u003cspan\u003eIn standard reaction, one unit is defined as the amount of enzyme that will incorporate 1 nmol ATP into acid-insoluble material in a total reaction volume of 50 μl in 1 hour at 37°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch4\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/h4\u003e\n\u003ctable style=\"width: 100%;\" data-mce-style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 60%;\" data-mce-style=\"width: 60%;\"\u003e\u003cstrong\u003eComponent Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%;\" data-mce-style=\"width: 20%;\"\u003e\u003cstrong\u003eAmount\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%;\" data-mce-style=\"width: 20%;\"\u003e\u003cstrong\u003eStorage (°C)\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003e\u003cspan\u003eT7 RNA Polymerase (50 U\/μl)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e100 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003e\u003cspan\u003e5 x T7 Transcription Buffer\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e1 ml\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003eRestrictions\u003cbr\u003e\n\u003c\/h4\u003e\n\u003cp\u003e\u003cspan\u003eFor Research Use Only.\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42892653560020,"sku":"FG-R00301","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder_5711bff0-2303-4871-a291-c01d2b548a4a.jpg?v=1656612594"},{"product_id":"firegene-eia-basic-kit","title":"FireGene EIA Basic Kit","description":"\u003cp\u003eThe FireGene EIA Basic Kit is a basic isothermal amplification kit with DNA or cDNA as the template. It can be used for nucleic acid electrophoresis detection and has the advantages of high amplification yield, strong specificity and good stability. Combined with specific probes designed for the target gene, using enzymes such as EXO III or Nfo for specific recognition and hydrolysis of the probe, the target gene can be accurately quantified and detected, with good repeatability and high reliability. The minimum detection limit of this kit is 10~100 copies (according to different primer screening optimization procedures and detection methods).\u003c\/p\u003e\n\u003ch4\u003eApplications\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eNucleic acid electrophoresis detection\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/h4\u003e\n\u003ctable style=\"width: 100%;\" data-mce-style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 60%;\" data-mce-style=\"width: 60%;\"\u003e\u003cstrong\u003eComponent Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%;\" data-mce-style=\"width: 20%;\"\u003e\u003cstrong\u003eAmount\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%;\" data-mce-style=\"width: 20%;\"\u003e\u003cstrong\u003eStorage (°C)\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003eEIA Basic Enzyme Mix\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e530 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003eEIA Basic Reaction Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e1 ml\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003e\u003cspan\u003eActivator\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e200 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003e\u003cspan\u003eForward primer (F)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e24 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e-20\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003e\u003cspan\u003eReverse primer (R)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e24 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e-20\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003e\u003cspan\u003ePositive control\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e20 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e-20\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003e\u003cspan\u003e6 x DNA Loading Buffer\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e500 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e-20\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003eRestrictions\u003cbr\u003e\n\u003c\/h4\u003e\n\u003cp\u003e\u003cspan\u003eFor Research or Educational Use Only.\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42892686065876,"sku":"FG-M00101","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder_c94298bd-c0cb-4dc9-8599-0dc48b419e89.jpg?v=1656611343"},{"product_id":"firegen-one-step-rt-lamp-sybr-green-kit","title":"FireGene One Step RT-LAMP SYBR Green kit","description":"\u003cp\u003e\u003cspan data-mce-fragment=\"1\"\u003eThe FireGene One-Step RT-LAMP\u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003e SYBR Green kit is a reverse transcription loop-mediated isothermal amplification (RT-LAMP) based \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003ekit.\u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003e The RT-LAMP Enzyme Mix\u003c\/span\u003e contains the Bst DNA Polymerase and the M-MLV (H- ) Reverse Transcriptase (heat-resistant) as the key enzymes. It can be used for SYBR® Green I fluorescence quantification of LAMP with high sensitivity and strong specificity. Combined with the Buffer optimized for SYBR® Green I method, it can inhibit non-specific amplification, thereby significantly improving the amplification efficiency, and is suitable for high-sensitivity dye-based LAMP detection reactions. This product is provided in a convenient Mix, which can obtain a good standard curve in a wide range, and can accurately quantify and detect the target gene with high repeatability and reliability.\u003c\/p\u003e\n\u003ch4\u003eApplications\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eGene expression analysis of gene targets\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/h4\u003e\n\u003ctable style=\"width: 100%;\" data-mce-style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 60%;\" data-mce-style=\"width: 60%;\"\u003e\u003cstrong\u003eComponent Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%;\" data-mce-style=\"width: 20%;\"\u003e\u003cstrong\u003eAmount\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%;\" data-mce-style=\"width: 20%;\"\u003e\u003cstrong\u003eStorage (°C)\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003e\n\u003cspan\u003eRNase free ddH\u003c\/span\u003e\u003csub\u003e2\u003c\/sub\u003e\u003cspan\u003eO\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e1.25 ml x 4\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003e\n\u003cspan\u003e2 x LAMP SYBR Reaction Mix\u003c\/span\u003e\u003csup\u003ea\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e1.25 ml x 4\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003e\n\u003cspan\u003eRT LAMP Enzyme Mix\u003c\/span\u003e\u003csup\u003eb\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e125 μl x 4\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003ea. Contains dNTPs, Mg2+ and SYBR® Green I\u003c\/p\u003e\n\u003cp\u003eb. Contains Bst DNA Polymerase, M-MLV (H- ) Reverse Transcriptase (heat-resistant), RNasin, etc.\u003c\/p\u003e\n\u003ch4\u003eRestrictions\u003cbr\u003e\n\u003c\/h4\u003e\n\u003cp\u003e\u003cspan\u003eFor Research or Educational Use Only.\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv id=\"gtx-trans\" style=\"position: absolute; left: -54px; top: 566.223px;\"\u003e\n\u003cdiv class=\"gtx-trans-icon\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42895928525012,"sku":"FG-M00202","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder_58660dbf-b20d-4ccc-8425-47831360f76a.jpg?v=1656610396"},{"product_id":"firegene-1st-strand-cdna-synthesis-kit-gdna-wiper","title":"FireGene 1st Strand cDNA Synthesis Kit (+gDNA wiper)","description":"\u003cp\u003e\u003cspan data-mce-fragment=\"1\"\u003eThe FireGene 1st strand cDNA Synthesis Kit contains all of the reagents necessary for synthesis of full-length first-strand cDNA from total or poly A\u003c\/span\u003e\u003csup data-mce-fragment=\"1\"\u003e+\u003c\/sup\u003e\u003cspan data-mce-fragment=\"1\"\u003e RNA, including both oligo(dT) and random hexamers primers. This cDNA synthesis kit also works well with other cDNA-dependent techniques, including RT-PCR, cDNA probe preparation, quantitative PCR (qPCR), and PCR based cloning. \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eThe FireGene 1st strand cDNA Synthesis Kit is ideal for reverse transcription of many different RNA templates, including GC-rich templates and RNAs with high levels of secondary structure.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan data-mce-fragment=\"1\"\u003eThis kit includes 4 x gDNA wiper Mix that can completely remove residual genomic DNA contamination and ensure more reliable subsequent quantitative results, 2 x RT Mix that contains optimized buffer system and dNTP, and the Enzyme Mix contains FireGene Reverse Transcriptase and RNasin. You may also choose Oligo (dT)23VN, Random hexamers, or gene-specific primers as reverse transcription primers based on your requirements.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch4\u003eApplications\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eFirst-strand cDNA synthesis\u003c\/li\u003e\n\u003cli\u003ecDNA probe preparation\u003c\/li\u003e\n\u003cli\u003eRT-PCR\u003c\/li\u003e\n\u003cli\u003eSynthesis of cDNA libraries with a high proportion of full-length cDNAs\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/h4\u003e\n\u003ctable style=\"width: 100%;\" data-mce-style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 60%;\" data-mce-style=\"width: 60%;\"\u003e\u003cstrong\u003eComponent Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%;\" data-mce-style=\"width: 20%;\"\u003e\u003cstrong\u003eAmount\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%;\" data-mce-style=\"width: 20%;\"\u003e\u003cstrong\u003eStorage (°C)\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003e\n\u003cspan\u003eRNase free ddH\u003c\/span\u003e\u003csub\u003e2\u003c\/sub\u003e\u003cspan\u003eO\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e1 ml x 2\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003e\n\u003cspan\u003e2 x RT Mix\u003c\/span\u003e\u003csup\u003e*1\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e1 ml\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003e\n\u003cspan\u003eEnzyme Mix\u003c\/span\u003e\u003csup\u003e*2\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e200 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003e\u003cspan\u003eOligo (dT)\u003csub\u003e23\u003c\/sub\u003eVN (50 μM)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e100 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003e\u003cspan\u003eRandom hexamers (50 ng\/μl)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e100 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003e\u003cspan\u003e4 x gDNA wiper Mix\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e40 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e*1. Each contains 1 mM dNTP\u003c\/p\u003e\n\u003cp\u003e*2. Contains RNasin\u003c\/p\u003e\n\u003ch4\u003eRestrictions\u003cbr\u003e\n\u003c\/h4\u003e\n\u003cp\u003e\u003cspan\u003eFor Research or Educational Use Only.\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42896209936596,"sku":"FG-R00112","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FG0431-Main.jpg?v=1656608484"},{"product_id":"firegene-rt-mix-for-qpcr-gdna-wiper","title":"FireGene RT mix for qPCR (+gDNA wiper)","description":"\u003cp data-mce-fragment=\"1\"\u003e\u003cspan\u003eThe FireGene RT mix for qPCR (gDNA wiper) is suitable for two-step qRT-PCR detection. It can react quickly by simply adding template RNA and water. The \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eincluded\u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\u003cspan\u003egDNA wiper Mix \u003c\/span\u003e\u003cspan\u003ecan completely remove the residual genomic DNA contamination and ensure more reliable subsequent quantification results. The T Mix contains all the components required for the reverse transcription reaction, and stops the gDNA wiper function at the same time to ensure the \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003ecompleteness of \u003c\/span\u003e\u003cspan\u003ecDNA. This product is \u003cspan style=\"font-size: 14px;\" data-mce-style=\"font-size: 14px;\"\u003especifically\u003c\/span\u003e optimized for qPCR. The ratio-optimized Random primers\/Oligo dT primer mix enables cDNA synthesis to start from each region of the RNA transcript with the same reverse transcription efficiency, ensuring the reliability and repeatability of qPCR results. The reverse transcription product is compatible with SYBR® Green and probe-based qPCR. According to the purpose of the experiment, the corresponding reagents can be selected for high-performance gene expression analysis.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch4 data-mce-fragment=\"1\"\u003eApplications\u003c\/h4\u003e\n\u003cul data-mce-fragment=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003eFirst-strand cDNA synthesis\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003ecDNA probe preparation\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003eRT-PCR\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003eSynthesis of cDNA libraries with a high proportion of full-length cDNAs\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4 data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eComponents\u003c\/span\u003e\u003c\/h4\u003e\n\u003ctable style=\"width: 100%;\" data-mce-style=\"width: 100%;\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 60%;\" data-mce-style=\"width: 60%;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eComponent Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%;\" data-mce-style=\"width: 20%;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eAmount\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%;\" data-mce-style=\"width: 20%;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage (°C)\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\" data-mce-fragment=\"1\"\u003e\n\u003cspan data-mce-fragment=\"1\"\u003eRNase free ddH\u003c\/span\u003e\u003csub data-mce-fragment=\"1\"\u003e2\u003c\/sub\u003e\u003cspan data-mce-fragment=\"1\"\u003eO\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\" data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e1 ml x 2\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\" data-mce-fragment=\"1\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\" data-mce-fragment=\"1\"\u003e\n\u003cspan data-mce-fragment=\"1\"\u003e5 x RT Mix\u003c\/span\u003e\u003csup data-mce-fragment=\"1\"\u003e*1\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\" data-mce-fragment=\"1\"\u003e\u003cspan\u003e400 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\" data-mce-fragment=\"1\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\" data-mce-fragment=\"1\"\u003e\n\u003cspan data-mce-fragment=\"1\"\u003eControl No RT Mix\u003c\/span\u003e\u003csup data-mce-fragment=\"1\"\u003e*2\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\" data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e40 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\" data-mce-fragment=\"1\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\" data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e4 x gDNA wiper Mix\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\" data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e400 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\" data-mce-fragment=\"1\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e*1. Contains FireGene Reverse Transcriptase, dNTP, RNasin, Random primers\/Oligo dT primer mix.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e*2. The ingredients are the same as 5 x RT Mix, except that FireGene Reverse Transcriptase is not included.\u003c\/p\u003e\n\u003ch4 data-mce-fragment=\"1\"\u003eRestrictions\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/h4\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eFor Research or Educational Use Only.\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42896340058324,"sku":"FG-R00113","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder_d43c590f-fb9c-4544-87ad-b6fec046b56b.jpg?v=1656611950"},{"product_id":"firegene-fast-site-directed-mutagenesis-kits","title":"FireGene Fast Site-Directed Mutagenesis Kits","description":"\u003cp\u003eThe FireGene Fast Mutagenesis Kit is a site-directed mutagenesis system based on our rapid cloning technology. Using this kit, the target plasmid amplification product is be retreived by gel or DpnI digestion, the site-directed mutagenesis can be accomplished by direct transformation after recombination circularization. The kit consists of two modules: the Max Master Mix Amplification Module and the Rapid Cloning Module. The ultra-high fidelity of Max Master Mix significantly reduces the possibility of introducing new mutations during the amplification process, and it is conducive to the complete degradation of the original methylated template due to the extremely low amount of template used. Its excellent ability to amplify long fragments is widely applicable to the amplification of any plasmids less than 20 kb in length. The rapid cloning system utilizes an efficient homologous recombination reaction, so that when the site-directed mutagenesis kit performs DNA site-directed mutagenesis, the linearized PCR product can be efficiently circularized, which is beneficial to transform the host and improve the mutation efficiency.\u003c\/p\u003e\n\u003ch4\u003eApplications\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eDirectional cloning into virtually any vector at any site\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/h4\u003e\n\u003ctable style=\"width: 100%;\" data-mce-style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 60%;\" data-mce-style=\"width: 60%;\"\u003e\u003cstrong\u003eComponent Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%;\" data-mce-style=\"width: 20%;\"\u003e\u003cstrong\u003eAmount\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%;\" data-mce-style=\"width: 20%;\"\u003e\u003cstrong\u003eStorage (°C)\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003e\u003cspan\u003eDpnI\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e20 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003e\u003cspan\u003eUvsXase\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e20 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003e\u003cspan\u003eUFO Buffer\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e40 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003eRestrictions\u003cbr\u003e\n\u003c\/h4\u003e\n\u003cp\u003e\u003cspan\u003eFor Research or Educational Use Only.\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv id=\"gtx-trans\" style=\"position: absolute; left: 38px; top: 382.55px;\"\u003e\n\u003cdiv class=\"gtx-trans-icon\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42896457367764,"sku":"FG-C00201","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder_4d74ec5e-476c-4631-bc3c-ec7e1e6b0062.jpg?v=1656611399"},{"product_id":"firegene-one-step-cloning-kit","title":"FireGene One Step Cloning Kit","description":"\u003cp data-mce-fragment=\"1\"\u003eFireGene One Step Cloning technology is a Fast, Efficient, and simple DNA directional cloning technology, which can directionally clone the PCR product of the target gene to any site of any vector. FireGene One Step Cloning Kit is a new generation recombination cloning kit with the core of recombinase obtained by heterologous expression of a gene of T4 phage. UvsXase is compatible with conventional digestion and PCR reaction systems. Cloning vector restriction products or PCR products and target gene PCR products can be directly used for recombinant cloning without DNA purification, which greatly simplifies the experimental steps.\u003c\/p\u003e\n\u003ch4 data-mce-fragment=\"1\"\u003eApplications\u003c\/h4\u003e\n\u003cul data-mce-fragment=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003eDNA site-directed mutagenesis\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003eHigh-throughput cloning\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003eSeamless cloning\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003eFast cloning\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4 data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eComponents\u003c\/span\u003e\u003c\/h4\u003e\n\u003ctable style=\"width: 100%;\" data-mce-fragment=\"1\" data-mce-style=\"width: 100%;\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 60%;\" data-mce-fragment=\"1\" data-mce-style=\"width: 60%;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eComponent Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%;\" data-mce-fragment=\"1\" data-mce-style=\"width: 20%;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eAmount\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%;\" data-mce-fragment=\"1\" data-mce-style=\"width: 20%;\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage (°C)\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 50%;\" data-mce-fragment=\"1\" data-mce-style=\"width: 50%;\"\u003e\u003cspan\u003eUvsXase\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-fragment=\"1\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e50 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-fragment=\"1\" data-mce-style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 50%;\" data-mce-fragment=\"1\" data-mce-style=\"width: 50%;\"\u003e\u003cspan\u003e5 xUFO Buffer\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-fragment=\"1\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e100 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-fragment=\"1\" data-mce-style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003ch4 data-mce-fragment=\"1\"\u003eExperiment Workflow\u003c\/h4\u003e\n\u003cp\u003eBasic Steps:\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eAmplify the whole plasmid of the vector at the cloning site to obtain a linearized vector;\u003c\/li\u003e\n\u003cli\u003eDesign homologous primers so that the 5' and 3' ends of the PCR product of the target fragment have the c\u003cspan data-mce-fragment=\"1\"\u003eompletely identical sequence as the \u003c\/span\u003efront and rear ends of the pre-inserted linearized vector, respectively (15 bp ~ 20 bp);\u003c\/li\u003e\n\u003cli\u003eAfter mixing the amplified PCR product of the target gene and the linearized vector in a certain proportion, under the catalysis of UvsXase, the transformation can be carried out within 30 minutes, and the directional cloning is completed.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eThe positive rate of clones can reach more than 95%.\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"Flow chart of single gene cloning experiment using UvsXase\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/files\/Flow_chart_of_single_gene_cloning_experiment_using_UvsXase_480x480.png?v=1654717278\"\u003e\u003c\/p\u003e\n\u003ch4 data-mce-fragment=\"1\"\u003eRestrictions\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/h4\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eFor Research or Educational Use Only.\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42896681664724,"sku":"FG-C00101","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder_b50f057f-0f30-482d-9abf-ca2bc6443b42.jpg?v=1656611716"},{"product_id":"magicscript-ii-first-strand-cdna-synthesis-kit","title":"MagicScript II First Strand cDNA Synthesis Kit","description":"\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eMagicScript II cDNA First Strand Synthesis Kit features two optimized mixes, MagicScript II Enzyme Mix and MagicScript II Reaction Mix. The enzyme mix combines MagicScript II Reverse Transcriptase and RNase Inhibitor, while the reaction mix contains an optimized buffer. MagicScript II Reverse Transcriptase is a recombinant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability. It can be used to synthesize first strand cDNA at higher temperatures than the wild type M-MuLV. The enzyme is active up to 50°C, providing higher specificity and higher yield of cDNA.\n             \n             The kit also provides two optimized primers for reverse transcription and nuclease-free water. An anchored Oligo-dT primer [d(T)23VN] forces the primer to anneal to the beginning of the polyA tail. The optimized Random Primer Mix provides random and consistent priming sites covering the entire RNA templates including both mRNAs and non-polyadenylated RNAs. The first strand cDNA product generated is up to 10 kb.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003ecDNA Synthesis; RT-PCR \u0026amp; cDNA Synthesis; Reverse Transcription (cDNA Synthesis)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e50 rxn \/ 100 rxn\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e* MagicScript II Reaction Mix (2X)\n             \n             * MagicScript II Enzyme Mix (10X)\n             \n             * Oligo d(T)23VN (50 uM)\n             \n             * dNTPs (10 mM)\n             \n             * Nuclease-free H2O\n             \n             * Random Primer Mix (60 uM)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eFor Research Use Only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eStore the MagicScript II First Strand cDNA Synthesis Kit at -20°C. Please avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003ch4\u003eRestrictions\u003c\/h4\u003e\n\u003cp\u003e\u003cspan\u003eFor Research Use Only.\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42990250557652,"sku":"FG-ENY101","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder_a2bdb91f-0bc3-4672-98ae-70a0ca759270.jpg?v=1657059522"},{"product_id":"magicscript-ii-rt-master-mix-for-qpcr","title":"MagicScript II RT Master Mix for qPCR","description":"\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eMagicScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) with reduced RNase H activity and increased thermal stability compared to wild-type MMLV RT. MagicScript II RT Master Mix for qPCR is a reverse transcription detection reagent suitable for two-step RT-qPCR. The 5X MagicScript II RT Mix contains all the reagents required for Reverse transcription reactions [MagicScript II Reverse Transcriptase, dNTP, RNase Inhibitor, Random primers\/Oligo (dT)23VN Primer Mix and optimized reaction solution]. The volume of RNA template can be up to 80% of the total volume, which is very suitable for the reverse transcription reaction of low concentration RNA template. 5 x MagicScript II RT Mix will not freeze at -20°C storage, which is very convenient to use.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003ecDNA Synthesis; RT-PCR \u0026amp; cDNA Synthesis; Reverse Transcription (cDNA Synthesis)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e10 rxn \/ 100 rxn\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e5x\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eNuclease-free H2O; 5X MagicScript II RT Mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eFor Research Use Only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eStore the MagicScript II RT Master Mix for qPCR at -20°C. Please avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42990250623188,"sku":"FG-ENY102","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder_75183d25-c3be-4d8f-b6ba-070f5cef0cce.jpg?v=1657059523"},{"product_id":"t4-polynucleotide-kinase","title":"T4 Polynucleotide Kinase","description":"\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eT4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer and exchange of Pi from the γ position of ATP to the 5' -hydroxyl terminus of polynucleotides (double-and single-stranded DNA and RNA) and nucleoside 3'-monophosphates. T4 Polynucleotide Kinase also catalyzes the removal of 3'-phosphoryl groups from 3'-phosphoryl polynucleotides, deoxynucleoside 3'-monophosphates and deoxynucleoside 3'-diphosphates. T4 PNK is applicable to end-labeling DNA or RNA for probes and DNA sequencing, addition of 5'-phosphates to oligonucleotides to allow subsequent ligation and removal of 3'-phosphoryl groups.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eA E. coli strain that carries the cloned T4 Polynucleotide Kinase gene.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003ePhosphorylation (Kinase)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e250 U \/ 500 U \/ 2500 U\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e10,000 U\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eT4 Polynucleotide Kinase (10,000 U\/ml)ï¼›10X T4 PNK Reaction Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eOne Richardson unit is defined as the amount of enzyme catalyzing the incorporation of 1 nmol of acid-insoluble [32P] in a total reaction volume of 50 μl in 30 minutes at 37°C in 1X T4 PNK Reaction Buffer with 66 µM [γ-32P] ATP (5 x 106 cpm\/µmol) and 0.26 mM 5'-hydroxyl-terminated salmon sperm DNA.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eFor Research Use Only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eStore the T4 Polynucleotide Kinase at -20°C. Please avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42990250655956,"sku":"FG-ENY103","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder_75eda2c5-0528-4ab3-a333-69003338efff.jpg?v=1657059525"},{"product_id":"dna-polymerase-i-large-klenow-fragment","title":"DNA Polymerase I, Large (Klenow) Fragment","description":"\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eDNA Polymerase I, Large (Klenow) Fragment (about 68 kD) is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3'\u0026amp;rightarrow;5' exonuclease activity, but has lost 5'\u0026amp;rightarrow;3' exonuclease activity. Klenow retains the polymerization fidelity of the holoenzyme without degrading 5' termini.  It is applicable to DNA sequencing by the Sanger dideoxy method, fill-in of 5' overhangs to form blunt ends, removal of 3' overhangs to form blunt ends, second strand cDNA synthesis and second strand synthesis in mutagenesis protocols. Theoretical protein molecular weight is 68000 daltons. Error Rate: ~ 18x10-6 bases\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eAn E.coli strain that contains the E. coli polA gene that has had its 5'\u0026amp;rightarrow;3' exonuclease domain removed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eBlunting, DNA Sequencing , Polymerases for DNA Manipulation,  RT-qPCR, RT-PCR and cDNA Synthesis, RT-PCR \u0026amp; cDNA Synthesis, PCR\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e200 U \/ 1000 U \/ 5000 U\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e5,000 U\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eDNA Polymerase I, Large (Klenow) Fragment (5,000 U\/ml); 10X ABuffer B\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eOne unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eFor Research Use Only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eStorage condition: 25 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 @25°C. Store the DNA Polymerase I, Large (Klenow) Fragment at -20°C. Please avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42990250688724,"sku":"FG-ENY104","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder_d9c7b7d5-7883-4e1b-9b31-2b389de52e13.jpg?v=1657059526"},{"product_id":"uracil-dna-glycosylase-udg","title":"Uracil-DNA Glycosylase (UDG)","description":"\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eE.coli Uracil-DNA Glycosylase (UDG) catalyses the release of free uracil from uracil-containing DNA. UDG efficiently hydrolyzes uracil from single-stranded or double-stranded DNA, but not from oligomers (6 or fewer bases). It releases uracil from ss- or ds-DNA and is applicable to eliminates PCR carry-over contamination.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eAn E.coli strain that carries the UDG gene from E. coli .\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eEliminates PCR carry-over contamination\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e1000 U \/ 5000 U\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e5,000 U\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eUDG (5,000 U\/ml); 10X UDG Reaction Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eOne unit is defined as the amount of enzyme that catalyzes the release of 60 pmol of uracil per minute from double-stranded, uracil-containing DNA. Activity is measured by release of [3H]-uracil in a 50 µl reaction containing 0.2 μg DNA (104-105 cpm\/μg) in 30 minutes at 37°C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eFor Research Use Only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eStorage Conditions: 10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.1 mg\/ml BSA, 50% Glycerol, pH 7.4 @ 25°C. Store the Uracil-DNA Glycosylase (UDG) at -20°C. Please avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42990250754260,"sku":"FG-ENY105","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder_9884ee01-dca9-4104-ba36-fcaf7c9f3000.jpg?v=1657059527"},{"product_id":"t4-dna-polymerase","title":"T4 DNA Polymerase","description":"\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eT4 DNA Polymerase catalyzes the synthesis of DNA in the 5'\u0026amp;rightarrow;3' direction and requires the presence of template and primer. This enzyme has a 3'\u0026amp;rightarrow;5' exonuclease activity which is much more active than that found in DNA Polymerase I (E. coli). Unlike E. coli DNA Polymerase I, T4 DNA Polymerase does not have a 5'\u0026amp;rightarrow;3' exonuclease function. It is applicable to 3' overhang removal to form blunt ends, 5' overhang fill-in to form blunt ends, single strand deletion subcloning, second strand synthesis in site-directed mutagenesis and probe labeling using replacement synthesis. Theoretical protein molecular weight is 104000 daltons. Error Rate:  ~ 1x10-6 bases\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003ePurified from a strain of E. coli that carries the T4 DNA Polymerase gene.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eBlunting, PCR, Polymerases for DNA Manipulation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e200 U \/ 1000 U\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e5,000 U\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eT4 DNA Polymerase (5,000 U\/ml); 10X ABuffer B\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eOne unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eFor Research Use Only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eStorage Conditions: 100 mM KPO4, 1 mM DTT, 50% Glycerol, pH 6.5 @ 25°C. Store the T4 DNA Polymerase at -20°C. Please avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42990250819796,"sku":"FG-ENY106","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder_5b4d9869-ade8-4a96-9db7-99e8f8cebdb6.jpg?v=1657059528"},{"product_id":"hotstart-taq-2x-pcr-master-mix","title":"HotStart Taq 2X PCR Master Mix","description":"\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eHotStart Taq DNA polymerase is an innovative antibody-modified thermostable enzyme. The activity of the enzyme is completely closed at room temperature, avoiding the non-specific amplification and primer dimer in the preparation of the sample and the first cycle reaction temperature rise, increasing the specificity of DNA amplification. HotStart Taq 2x Master Mix is an optimized ready-to-use solution containing HotStart Taq DNA Polymerase, dNTPs, MgCl2, KCI and stabilizers. It is ideally suited to routine PCR applications from templates including pure DNA solutions, bacterial colonies, and cDNA products. It can amplify up to 4 kb from complex genomic DNA or up to 5 kb from lambda DNA. Applicable to the PCR reaction, colony PCR, primer extension, etc.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eMultiplex PCR,  PCR , Specialty PCR, Routine PCR\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e100 RXN \/ 500 RXN\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e2x\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eHotStart Taq 2X PCR Master Mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eFor Research Use Only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eStore the HotStart Taq 2X PCR Master Mix at -20°C. Please avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42990250983636,"sku":"FG-ENY109","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder_1f9b8494-a342-4e52-bad2-1953ced452dd.jpg?v=1657059532"},{"product_id":"magictaq-dna-polymerase","title":"MagicTaq DNA Polymerase","description":"\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eMagicTaq DNA Polymerase is an optimized blend of Taq Klenow and Deep Oean DNA polymerases for use with routine and difficult PCR experiments. The 3'\u0026amp;rightarrow; 5' exonuclease activity of Deep Ocean DNA Polymerase increases the fidelity and robust amplification of Taq klenow DNA Polymerase. The Magic Taq Reaction Buffers and High GC Enhancer have been formulated for robust yields with minimal optimization, regardless of a template's GC content. Magic Taq DNA Polymerase is supplied with two 5X buffers: (Standard and GC), as well as a High GC Enhancer solution. For most routine and\/or AT-rich amplicons (Lambda, etc.) or complex amplicons with up to ~50% GC content, Magic Taq Standard Reaction Buffer provides robust amplification. For GC-rich amplicons, the First Taq GC Reaction Buffer can improve both performance and yield. For particularly high GC or difficult amplicons, the First Taq High GC Enhancer can be added at a final concentration of 10–20% to reactions containing FirstTaq GC Reaction Buffer.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eMultiplex PCR,  PCR , Specialty PCR, Routine PCR\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e1000 U \/ 5000 U\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e5,000 U\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eDNA Polymerase (5,000 U\/ml); 5X GC Reaction Buffer; 5X Standard Reaction Buffer; 100% High GC Enhancer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eFor Research Use Only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eStorage Conditions: 10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% Tween 20, 0.5% IGEPAL CA-630, 50% Glycerol, pH7.4 @ 25°C. Store DNA Polymerase at -20°C. Please avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42990251049172,"sku":"FG-ENY110","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder_f355307d-bf76-4868-ba68-df82d4d753eb.jpg?v=1657059533"},{"product_id":"magicfast-pfu-2x-pcr-master-mix","title":"MagicFast Pfu 2X PCR Master Mix","description":"\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003ePfu DNA Polymerase is a high-fidelity, thermostable enzyme isolated from Pyrococcus furiosus. Pfu DNA Polymerase possesses 3'\u0026amp;rightarrow;5' exonuclease (proofreading) activity. Base misincorporations that may occur during polymerization are rapidly excised by this proofreading activity. \nMagicFast Pfu DNA Polymerase has been genetically engineered to enhance the amplification efficiency by fusing DNA binding domains to the Pfu DNA polymerase. MagicFast Pfu DNA Polymerase has the characteristics of high amplification speed (extension ~2 kb\/min), high fidelity and strong anti-interference ability.\n             \n             Consequently, MagicFast Pfu DNA Polymerase is recommended for use in PCR and primer extension reactions that require high-fidelity synthesis. MagicFast Pfu DNA Polymerase-generated PCR fragments are blunt-ended. MagicFast Pfu 2X PCR Master Mix is an optimized ready-to-use solution containing MagicFast Pfu DNA Polymerase, dNTPs, MgCl2, KCI and stabilizers.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eHigh Fidelity PCR, PCR , Routine PCR\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e100 rxn \/ 500 rxn\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e2x\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eMagicFast Pfu 2X PCR Master Mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eFor Research Use Only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eStore the MagicFast Pfu 2X PCR Master Mix at -20°C. Please avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42990251376852,"sku":"FG-ENY116","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder_319242a2-e60c-4d6d-81ff-fc922ea4ed00.jpg?v=1657059543"},{"product_id":"magicfast-pfu-2x-pcr-master-mix-with-dye","title":"MagicFast Pfu 2X PCR Master Mix with Dye","description":"\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003ePfu DNA Polymerase is a high-fidelity, thermostable enzyme isolated from Pyrococcus furiosus. Pfu DNA Polymerase possesses 3'\u0026amp;rightarrow;5' exonuclease (proofreading) activity. Base misincorporations that may occur during polymerization are rapidly excised by this proofreading activity. MagicFast Pfu DNA Polymerase has been genetically engineered to enhance the amplification efficiency by fusing DNA binding domains to the Pfu DNA polymerase. MagicFast Pfu DNA Polymerase has the characteristics of high amplification speed (extension ~2 kb\/min), high fidelity and strong anti-interference ability.\n             \n             Consequently, MagicFast Pfu DNA Polymerase is recommended for use in PCR and primer extension reactions that require high-fidelity synthesis. MagicFast Pfu DNA Polymerase-generated PCR fragments are blunt-ended. MagicFast Pfu 2X PCR Master Mix is an optimized ready-to-use solution containing MagicFast Pfu DNA Polymerase, dNTPs, MgCl2, KCI and stabilizers, as well as two commonly used tracking dyes for DNA gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at ~4 kb and Tartrazine migrates at ~10 bp. Both dyes are present in concentrations that do not mask comigrating DNA bands.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eHigh Fidelity PCR, PCR , Routine PCR\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e100 rxn \/ 500 rxn\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e2x\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eMagicFast Pfu 2X PCR Master Mix with Dye\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eFor Research Use Only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eStore the MagicFast Pfu 2X PCR Master Mix with dye at -20°C. Please avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42990251442388,"sku":"FG-ENY117","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder_8913e771-8ee0-49b7-840e-55ad095216c8.jpg?v=1657059546"},{"product_id":"magiclamp-taq-dna-polymerase","title":"FireAmp FastPfu Fly DNA Polymerase High-Fidelity DNA Polymerase","description":"\u003cp style=\"vertical-align: middle; text-align: left; text-indent: 0.0pt; line-height: 100.0%;\"\u003e\u003cspan\u003e\u003cb\u003e[Product Introduction]\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"vertical-align: middle; text-align: left; text-indent: 0.2pt; line-height: 100.0%;\"\u003e\u003cspan\u003eFireAmp FastPfu Fly DNA Polymerase is a hot-start ultra-high-fidelity DNA polymerase \u003c\/span\u003e\u003cspan\u003edesigned for rapid PCR. Compared with conventional high-fidelity DNA polymerases,this \u003c\/span\u003e\u003cspan\u003eenzyme features faster amplification speed (≤5 kb fragments can be amplified at an ultra-fast rate \u003c\/span\u003e\u003cspan\u003eof 12 kb\/min; \u0026gt;5 kb fragments can be amplified at a high speed of 6 kb\/min), higher \u003c\/span\u003e\u003cspan\u003eamplification efficiency, higher yield, higher fidelity, and higher sensitivity. The 2×FireAmp \u003c\/span\u003e\u003cspan\u003eFastPfu Fly Reaction Mix already contains dNTPs. For DNA amplification, only the template, primers, water, and\u003c\/span\u003e\u003cspan\u003e FireAmp FastPfu Fly DNA Polymerase need to be added to adjust the reaction mix\u003c\/span\u003e\u003cspan\u003e to 1× concentration for the reaction. The amplification product is blunt-ended and \u003c\/span\u003e\u003cspan\u003ecan be directly used for cloning experiments.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left; text-indent: 0.0pt; line-height: 185.2%; margin-left: 0.0pt; mso-para-margin-left: 0.0pt; margin-top: 0.0pt; mso-para-margin-top: 0.0pt; margin-bottom: 0.0pt; mso-para-margin-bottom: 0.0pt; mso-add-space: auto;\"\u003e\u003cspan\u003e·Fidelity is 108 times that of conventional Taq DNA polymerase.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left; text-indent: 0.0pt; line-height: 185.2%; margin-left: 0.0pt; mso-para-margin-left: 0.0pt; margin-top: 0.0pt; mso-para-margin-top: 0.0pt; margin-bottom: 0.0pt; mso-para-margin-bottom: 0.0pt; mso-add-space: auto;\"\u003e\u003cspan\u003e·Amplification product is blunt-ended and can be directly used for cloning experiments.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left; text-indent: 0.0pt; line-height: 185.2%; margin-left: 0.0pt; mso-para-margin-left: 0.0pt; margin-top: 0.0pt; mso-para-margin-top: 0.0pt; margin-bottom: 0.0pt; mso-para-margin-bottom: 0.0pt; mso-add-space: auto;\"\u003e\u003cspan\u003e·Amplification of genomic DNA fragments (≤15 kb)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left; text-indent: 0.0pt; line-height: 185.2%; margin-left: 0.0pt; mso-para-margin-left: 0.0pt; margin-top: 0.0pt; mso-para-margin-top: 0.0pt; margin-bottom: 0.0pt; mso-para-margin-bottom: 0.0pt; mso-add-space: auto;\"\u003e\u003cspan\u003e·Amplification of plasmid DNA fragments (≤20 kb)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left; text-indent: 0.0pt; line-height: 185.2%; margin-left: 0.0pt; mso-para-margin-left: 0.0pt; margin-top: 0.0pt; mso-para-margin-top: 0.0pt; margin-bottom: 0.0pt; mso-para-margin-bottom: 0.0pt; mso-add-space: auto;\"\u003e\u003cspan\u003e\u003cb\u003e[Specification]\u003c\/b\u003e 250 units\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left; text-indent: 0.0pt; line-height: 185.2%; margin-left: 0.0pt; mso-para-margin-left: 0.0pt; margin-top: 0.0pt; mso-para-margin-top: 0.0pt; margin-bottom: 0.0pt; mso-para-margin-bottom: 0.0pt; mso-add-space: auto;\"\u003e \u003c\/p\u003e\n\u003cp style=\"vertical-align: middle; text-align: left; text-indent: 0.0pt; line-height: 100.0%;\"\u003e\u003cspan\u003e\u003cb\u003e[Features]\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Times New Roman;\"\u003e· Hot-start, high specificity\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Times New Roman;\"\u003e· High amplification efficiency\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Times New Roman;\"\u003e· Rapid, ultra-high fidelity\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Times New Roman;\"\u003e· High sensitivity\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Times New Roman;\"\u003e· High yield\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Times New Roman;\"\u003e[\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003eApplications\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Times New Roman;\"\u003e]\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Times New Roman;\"\u003e· Amplification of complex templates and high GC\/AT content templates\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Times New Roman;\"\u003e· Ultra-high-fidelity rapid PCR, blunt-end cloning, site-directed mutagenesis\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Times New Roman;\"\u003e· Long-fragment amplification\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"vertical-align: middle; text-align: left; text-indent: -256.4pt; line-height: 100.0%;\"\u003e\u003cspan\u003e\u003c!-- [if !vml]--\u003e\u003cimg width=\"108.5\" height=\"28.6\" src=\"data:image\/png;base64,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\"\u003e\u003c!--[endif]--\u003e\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eFG-FG2104 FireAmp FastPfu Fly         \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Times New Roman;\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003e\u003cb\u003e[Product Components]\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable cellspacing=\"0\" border=\"1\" class=\"MsoTableGrid\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"296\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"102\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"91\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"85\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"296\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eFireAmp FastPfu Fly DNA Polymerase\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"102\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e250 U×1\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"91\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e500 U×1\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"85\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e500 U×6\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"296\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e2×FireAmp FastPfu Fly Reaction Mix\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"102\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1 ml×3\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"91\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1.2 ml×5\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"85\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1.2 ml×30\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"296\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e50 mM MgSO₄\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"102\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e200 μl×1\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"91\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e400 μl×1\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"85\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1 ml×1\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"296\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e6×DNA Loading Buffer\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"102\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e500 μl×1\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"91\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1 ml×1\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"85\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1 ml×2\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"296\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003ePCR Stimulant\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"102\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e200 μl×1\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"91\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e400 μl×1\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"85\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1 ml×1\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp style=\"vertical-align: middle; text-align: left; text-indent: 0.0pt; line-height: 100.0%;\"\u003e \u003c\/p\u003e\n\u003cp style=\"vertical-align: middle; text-align: left; text-indent: 0.0pt; line-height: 100.0%;\"\u003e\u003cspan\u003e\u003cb\u003e[Recommended PCR System and Conditions (50 μl reaction system)]\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable cellspacing=\"0\" border=\"1\" class=\"MsoTableGrid\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"297\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eComponent\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"135\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eVolume\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"142\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eFinal Concentration\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"297\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eTemplate\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"135\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eVariable\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"142\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eAs required\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"297\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eForward Primer (10 μM)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"135\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1 μl\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"142\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e0.2 μM\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"297\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eReverse Primer (10 μM)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"135\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1 μl\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"142\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e0.2 μM\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"297\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e2×FireAmp FastPfu Fly Reaction Mix\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"135\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e25 μl\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"142\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1×\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"297\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eFireAmp FastPfu Fly DNA Polymerase\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"135\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1 μl\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"142\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e2.5 units\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"297\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eNuclease-free Water\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"135\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eVariable\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"142\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"297\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eTotal volume\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"135\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e50 μl\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"142\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp style=\"vertical-align: middle; text-align: left; text-indent: 0.0pt; line-height: 100.0%;\"\u003e \u003c\/p\u003e\n\u003cp style=\"vertical-align: middle; text-align: left; text-indent: 0.0pt; line-height: 100.0%;\"\u003e\u003cspan\u003e\u003cb\u003e[Optimized Parameters (50 μl reaction system)]\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable cellspacing=\"0\" border=\"1\" class=\"MsoTableGrid\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"288\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eTemplate\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"288\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eInput\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"288\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eGenomic DNA\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"288\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10-500 ng\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"288\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003ePlasmid DNA\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"288\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1-30 ng\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"288\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003ecDNA\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"288\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1-2 μl cDNA from RT reaction (50-500 ng RNA for RT reaction)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp style=\"vertical-align: middle; text-align: left; text-indent: 0.0pt; line-height: 100.0%;\"\u003e \u003c\/p\u003e\n\u003cp style=\"vertical-align: middle; text-align: left; text-indent: 0.0pt; line-height: 100.0%;\"\u003e\u003cspan\u003e\u003cb\u003e[PCR Reaction Protocol]\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable cellspacing=\"0\" border=\"1\" class=\"MsoTableGrid\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"192\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eNumber of Cycles\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"192\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eTemperature\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"192\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eTime\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"192\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1 cycle\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"192\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e98℃\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"192\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1 min\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"3\" valign=\"top\" width=\"192\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e30-35 cycles\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"192\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e98℃\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"192\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10 sec\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"192\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eTm-5℃\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"192\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e5 sec\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"192\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e72℃\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"192\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e6 or 12 kb\/min *\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"192\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1 cycle\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"192\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e72℃\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"192\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1 min\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp style=\"vertical-align: middle; text-align: left; text-indent: 0.0pt; line-height: 100.0%;\"\u003e\u003cspan\u003e*For fragments ≤ 5 kb, select 12 kb\/min; for fragments \u0026gt; 5 kb, select 6 kb\/min.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"vertical-align: middle; text-align: left; text-indent: 0.0pt; line-height: 100.0%;\"\u003e\u003cspan\u003e\u003cb\u003e[PCR Stimulant]\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"vertical-align: middle; text-align: left; text-indent: 0.1pt; line-height: 100.0%;\"\u003e\u003cspan\u003ePCR Stimulant is used to optimize the amplification of complex templates or high GC\/AT content templates. \u003c\/span\u003e\u003cspan\u003eIt significantly enhances amplification for Pfu series enzymes. The storage solution \u003c\/span\u003e\u003cspan\u003econcentration is 5×, and the working concentration can be adjusted between 0.5× and 2.0×.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"vertical-align: middle; text-align: left; text-indent: 0.0pt; line-height: 100.0%;\"\u003e\u003cspan\u003e\u003cb\u003e[Precautions]\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left; text-indent: 0.9pt; line-height: 120.0%; margin-left: 0.0pt; mso-para-margin-left: 0.0pt; margin-top: 0.0pt; mso-para-margin-top: 0.0pt; margin-bottom: 0.0pt; mso-para-margin-bottom: 0.0pt; mso-add-space: auto;\"\u003e\u003cspan\u003e·It is recommended to add FireAmp FastPfu Fly DNA polymerase to the reaction system as the \u003c\/span\u003e\u003cspan\u003elast step.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left; text-indent: 0.9pt; line-height: 120.0%; margin-left: 0.0pt; mso-para-margin-left: 0.0pt; margin-top: 0.0pt; mso-para-margin-top: 0.0pt; margin-bottom: 0.0pt; mso-para-margin-bottom: 0.0pt; mso-add-space: auto;\"\u003e\u003cspan\u003e·Please thaw the 2×FireAmp FastPfu Fly Reaction Mix completely before use.If a small amount \u003c\/span\u003e\u003cspan\u003eof precipitate forms, dissolve it by heating in a 37°C water bath and mix well before use.\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"250 units","offer_id":46130521768148,"sku":"FG-FG2104","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/files\/FireAmpFastPfuFlyDNAPolymeraseHigh-FidelityDNAPolymerase.png?v=1778574964"},{"product_id":"magiclamp-taq-2x-master-mix","title":"MagicLAmp Taq 2X Master Mix","description":"\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eMagicLAmp Taq 2X PCR Master Mix combines high quality recombinant Taq klenow DNA Polymerase with a trace amount of Deep Ocean DNA Polymerase. The 3'\u0026amp;rightarrow;5' exonuclease activity of Deep Ocean DNA Polymerase increases the fidelity and robust amplification of Taq klenow DNA Polymerase. MagicLAmp Taq DNA Polymerase offers two fold higher fidelity than Taq klenow DNA Polymerase alone. A wide range of PCR products can be generated, up to 30 kb from lambda DNA or from human genomic DNA.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eLong Range PCR, Specialty PCR, PCR , Routine PCR\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e100 RXN \/ 500 RXN\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e2x\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eMagicLAmp Taq 2X PCR Master Mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eFor Research Use Only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eStore the MagicLAmp Taq 2X PCR Master Mix at -20°C. Please avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42990251573460,"sku":"FG-ENY119","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder_5c47dff3-150c-41a1-ad02-c3120c349572.jpg?v=1657059549"},{"product_id":"magiclamp-taq-2x-master-mix-with-dye","title":"MagicLAmp Taq 2X Master Mix with Dye","description":"\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eMagicLAmp Taq 2X PCR Master Mix with Dye combines high quality recombinant Taq klenow DNA Polymerase with a trace amount of Deep Ocean DNA Polymerase. The 3'\u0026amp;rightarrow;5' exonuclease activity of Deep Ocean DNA Polymerase increases the fidelity and robust amplification of Taq klenow DNA Polymerase. MagicLAmp Taq DNA Polymerase offers two fold higher fidelity than Taq klenow DNA Polymerase alone. A wide range of PCR products can be generated, up to 30 kb from lambda DNA or from human genomic DNA.\n             \n             MagicLAmp Taq 2X PCR Master Mix with Dye is an optimized ready-to-use solution containing MagicLAmp Taq DNA Polymerase, dNTPs, MgCl2, KCI and stabilizers, as well as two commonly used tracking dyes for DNA gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at ~4 kb and Tartrazine migrates at ~10 bp. Both dyes are present in concentrations that do not mask comigrating DNA bands.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eLong Range PCR, Specialty PCR, PCR , Routine PCR\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e100 RXN \/ 500 RXN\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e2x\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eMagicLAmp Taq 2X PCR Master Mix with Dye\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eFor Research Use Only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eStore the MagicLAmp Taq 2X PCR Master Mix with Dye at -20°C. Please avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42990251606228,"sku":"FG-ENY120","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder_a8ba5d8f-004e-45a7-8f1b-aa78faaca65e.jpg?v=1657059550"},{"product_id":"magic-high-fidelity-dna-polymerase","title":"Magic High-Fidelity DNA Polymerase","description":"\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eMagic High-Fidelity DNA Polymerase is a novel B-family DNA polymerase, engineered to have increased affinity for DNA. The intrinsic high processivity of the enzyme results in significant improvement in yield, speed and sensitivity when compared with wild-type B-family DNA polymerases. In addition, the ability to amplify long targets, as well as GC- and AT-rich targets, is significantly improved. In addition, Magic High-Fidelity DNA Polymerase requires significantly shorter reaction times than wild-type B-family DNA polymerases.\n             \n             Magic High-Fidelity DNA Polymerase has 5’\u0026amp;rightarrow;3’ polymerase and 3’\u0026amp;rightarrow;5’ exonuclease (proofreading) activity, but no 5’\u0026amp;rightarrow;3’ exonuclease activity. The strong 3’\u0026amp;rightarrow;5’ exonuclease activity results in superior accuracy during DNA amplification, lending to Magic High-Fidelity DNA Polymerase the lowest published error rate of all B-family DNA polymerases (1 error per 3.6 x 106 nucleotides incorporated). This fidelity is approximately 100 times higher than that of wild-type Taq DNA polymerase, and up to 10 times higher than that of other B-family DNA polymerases and polymerase blends.\nDNA fragments generated with Magic High-Fidelity DNA Polymerase may be used for routine downstream analysis and applications, including restriction enzyme digestion, cloning and sequencing. PCR products generated with Magic High-Fidelity DNA Polymerase are blunt-ended, but may be 3’-dA-tailed for cloning into TA cloning vectors.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eAn E.coli strain carries the Magic High-Fidelity DNA Polymerase gene.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eHigh Fidelity PCR, High throughput PCR , Routine PCR\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e100 U \/ 1000 U \/ 5000 U\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e2,000 U\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eMagic High-Fidelity DNA Polymerase (2,000 U\/ml); 5X Magic High-Fidelity DNA Polymerase HF Buffer; 5X Magic High-Fidelity DNA Polymerase GC Buffer; MgCl2 Solution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eOne unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 74°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eFor Research Use Only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eStorage Conditions: 20 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 200 μg\/ml BSA, 50% Glycerol, 1X stabilizers, pH 7.4 @ 25°C. Store the Magic High-Fidelity DNA Polymerase at -20°C. Please avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42990251671764,"sku":"FG-ENY121","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder_fdfc16c6-df8a-46e6-a198-34cbf9d5de39.jpg?v=1657059552"},{"product_id":"magic-high-fidelity-2x-master-mix-for-ngs","title":"Magic High-Fidelity 2X Master Mix for NGS","description":"\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eMagic High-Fidelity DNA Polymerase is a novel B-family DNA polymerase, engineered to have increased affinity for DNA. The intrinsic high processivity of the enzyme results in significant improvement in yield, speed and sensitivity when compared with wild-type B-family DNA polymerases. In addition, the ability to amplify long targets, as well as GC- and AT-rich targets, is significantly improved. Magic High-Fidelity  DNA Polymerase has 5’\u0026amp;rightarrow;3’ polymerase and 3’\u0026amp;rightarrow;5’ exonuclease (proofreading) activity, but no 5’\u0026amp;rightarrow;3’ exonuclease activity. The strong 3’\u0026amp;rightarrow;5’ exonuclease activity results in superior accuracy during DNA amplification, lending to Magic High-Fidelity DNA Polymerase the lowest published error rate of all B-family DNA polymerases (1 error per 3.6 x 106 nucleotides incorporated). This fidelity is approximately 100 times higher than that of wild-type Taq DNA polymerase, and up to 10 times higher than that of other B-family DNA polymerases and polymerase blends.\n             \n             In Magic High-Fidelity  2X PCR Master Mix with HF Buffer, Magic High-Fidelity  DNA Polymerase is supplied in a convenient 2X Master Mix format, containing all reaction components except primers and template. The Master Mix contains Magic High-Fidelity DNA Polymerase (1 U per 50 µL reaction) in a proprietary reaction buffer containing dNTPs (0.3 mM of each dNTP at 1X), MgCl2 (1.5 mM at 1X) and stabilizers. Magic High-Fidelity 2X PCR Master Mix with HF Buffer is designed for routine, high-fidelity PCR of a wide range of targets and fragment sizes. In addition, Magic High-Fidelity  DNA polymerase requires significantly shorter reaction times than wild-type B-family DNA polymerases.\n             \n             DNA fragments generated with Magic High-Fidelity  2X PCR Master Mix may be used for routine downstream analysis and applications, including restriction enzyme digestion, cloning and sequencing. PCR products generated with Magic High-Fidelity  2X PCR Master Mix are blunt-ended, but may be 3’-dA-tailed for cloning into TA cloning vectors.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eHigh Fidelity PCR, High throughput PCR , Routine PCR\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e100 rxn \/ 500 rxn\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e2x\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eHigh-Fidelity 2X Master Mix for NGS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eFor Research Use Only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eStore the High-Fidelity 2X Master Mix for NGS at -20°C. Please avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42990251802836,"sku":"FG-ENY124","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder_755c85a1-e414-41d8-9c69-b0202fc889d7.jpg?v=1657059555"},{"product_id":"magicscript-ii-reverse-transcriptase","title":"MagicScript II Reverse Transcriptase","description":"\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eMagicScript II Reverse Transcriptase is a recombinant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability. It can be used to synthesize first strand cDNA at higher temperatures than the wild type M-MuLV. The enzyme is active up to 48°C, providing higher specificity, higher yield of cDNA and more full-length cDNA product up to 12 kb.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eThe gene encoding a mutant M-MuLV Reverse Transcriptase (RNase H-) is expressed in E. coli and purified to near homogeneity.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003ecDNA Synthesis, RT-qPCR, RT-PCR and cDNA Synthesis , PCR, Reverse Transcription (cDNA Synthesis), RT-PCR \u0026amp; cDNA Synthesis\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e4000 U \/ 10000 U\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e200,000 U\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eMagicScript II Reverse Transcriptase (200,000 U\/ml); 100 mM DTT (10X); 5X First-Strand Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eOne unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-insoluble material in a total reaction volume of 50 μl in 10 minutes at 37°C using poly(rA)•oligo(dT)18 as template.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eFor Research Use Only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eStorage Conditions: 20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, 0.01% IGEPAL CA-630, pH 7.5 @ 25°C. Store the MagicScript II Reverse Transcriptase at -20°C. Please avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42990251835604,"sku":"FG-ENY125","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder_a760d881-822d-46eb-a859-0d4e532a3989.jpg?v=1657059556"},{"product_id":"rnase-inhibitor-mammalian","title":"RNase Inhibitor, Mammalian","description":"\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eRNase Inhibitor, Mammalian specifically inhibits RNases A, B and C. It inhibits RNases by binding noncovalently in a 1:1 ratio with high affinity. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor is used with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3). The 50 kDa protein inhibits RNases by binding noncovalently in a 1:1 ratio with an association constant greater than 10^14.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eAn E.coli strain that carries the Ribonuclease Inhibitor gene from Porcine.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eCell-Free Protein Expression, PCR, RT-PCR,cDNA synthesis,In vitro transcription\/translation,Enzymatic RNA labeling reaction,Other applications where the integrity of RNA is important\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e2000 U \/ 10000 U \/ 20000 U\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e40,000 U\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eRNase Inhibitor, Mammalian (40,000 U\/ml)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eOne unit is defined as the amount of RNase Inhibitor, Mammalian required to inhibit the activity of 5ng of RNase A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2', 3'-cyclic monophosphate by RNase A.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eFor Research Use Only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eStore the RNase Inhibitor, Mammalian at -20°C. Please avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42990251868372,"sku":"FG-ENY126","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder_11491ea3-ce48-40fd-bc13-ba3a2847a649.jpg?v=1657059558"},{"product_id":"t4-dna-ligase-high-concentration","title":"T4 DNA Ligase (High Concentration)","description":"\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eT4 DNA Ligase can catalyze the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA and some DNA\/RNA hybrids. T4 DNA ligase will seal nicks for these DNA substrates. Applicable to Cloning of restriction fragments, joining linkers and adapters to blunt-ended DNA.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eAn E.coli strain that carries the T4 DNA ligase gene.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eCloning Ligation, Adaptor Ligation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e80000 U \/ 400000 U\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e2,000,000 U\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eT4 DNA Ligase (High Conc.); 10X T4 DNA ligase Reaction Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eOne unit is defined as the amount of enzyme required to give 50% ligase of HindIII fragments of λ DNA (5' DNA termini concentration of 0.12 µM, 300- μg\/ml) in a total reaction volume of 20 μl in 30 minutes at 16°C in 1X T4 DNA Ligase Reaction Buffer.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eFor Research Use Only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eStorage Conditions: 10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH7.4 @ 25°C. Store the T4 DNA Ligase (High Conc.) at -20°C. Please avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42990251933908,"sku":"FG-ENY127","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder_b6dcaa02-aa4b-437b-8dae-19a05291ece9.jpg?v=1657059559"},{"product_id":"tn5-transposase","title":"Tn5 Transposase","description":"\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eSynonym\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eTn5; Tn5 Transposase; Transposase (Tnp) Tn5; Dimer_Tnp_Tn5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eTn5 transposase is a bacterial enzyme that integrates a DNA fragment into genomic DNA, and is used as a tool for detecting nucleosome-free regions of genomic DNA in eukaryotes. Bacterial Tn5 transposase promotes the transposition of the Tn5 transposon. Tn5 transposase integrates DNA fragments at a specific site in the nucleosomal DNA. In the ATAC-seq method, Tn5 transposase has been employed to insert an adaptor DNA fragment in the nucleosome-free DNA regions and linker DNA regions between nucleosomes.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eMagic\u003cspan data-mce-fragment=\"1\"\u003e™\u003c\/span\u003e Tn5 Transposase is a naked and hyperactive form of Tn5 transposase. Our Magic Tn5 Transposase can recognize inside, outside and mosaic end sequences of Tn5 transposon.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eE.coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e\n\u003cul\u003e\n\u003cli\u003eLibrary construction for second-generation sequencing.\u003c\/li\u003e\n\u003cli\u003eIn vitro transgenic experiment.\u003c\/li\u003e\n\u003cli\u003eATAC-seq\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e25U\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e1 U\/μl.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003ea. Tn5 Transposase(1 U\/μl) b. 5x LM Buffer c. 10xTPS Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eOne unit Tn5 Transposase is defined as the amount of enzyme that cleaves 1 ug DNA fragment containing recognition seqence in 1 hour at 37°C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e50 mM Tris-HCl (pH 8.0), 100 mM NaCl, 0.1 mM EDTA, 0.1% Triton X-100, 50% glycerol (V\/V)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eFor Research Use Only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eThe Tn5 Transposase is shipped with dry ice. Store Tn5 Transposase at -20°C. Avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42990251966676,"sku":"FG-TN5BL01","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder_062be9d0-ce45-4a8e-bd03-3f19b7c4e348.jpg?v=1657059560"}],"url":"https:\/\/firegene.com\/collections\/pcr-qpcr-and-molecular-biology-reagents.oembed","provider":"FireGene","version":"1.0","type":"link"}