{"title":"Reverse Transcription and cDNA Synthesis","description":"\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003cstrong\u003eReverse Transcription and cDNA Synthesis\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003eThe RT step sits between your RNA and everything downstream — and it's where more variation enters the workflow than most protocols acknowledge. Template secondary structure, genomic DNA contamination, inhibitor carryover from extraction, primer bias: each of these affects cDNA yield and representation before the first qPCR cycle runs.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003eThis collection covers five products across three RT workflow formats, each addressing a different combination of input and downstream application.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003cstrong\u003eStandalone reverse transcriptase\u003c\/strong\u003e MagicScript II Reverse Transcriptase for labs that run their own optimized RT protocols — high thermostability for GC-rich or structured templates, compatible with oligo(dT), random hexamers, or gene-specific primers.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003cstrong\u003eFirst-strand cDNA synthesis kits\u003c\/strong\u003e Two formats: standard First Strand cDNA Synthesis Kit for clean RNA inputs, and a gDNA Wiper variant that removes genomic DNA contamination in the same tube before reverse transcription begins. No separate DNase incubation step, no additional reagent, no extra time — the wiper reagent is added first, incubated briefly, then the RT reaction proceeds in the same tube.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003cstrong\u003eRT mixes for qPCR\u003c\/strong\u003e Two RT Mix for qPCR formats — standard and gDNA Wiper — using a balanced random primer\/oligo(dT) ratio for uniform coverage across transcript lengths. Optimized for two-step RT-qPCR where the cDNA is used directly in downstream qPCR without cleanup.\u003c\/p\u003e","products":[{"product_id":"firegene-1st-strand-cdna-synthesis-kit-gdna-wiper","title":"FireGene 1st Strand cDNA Synthesis Kit (+gDNA wiper)","description":"\u003cp\u003e\u003cspan data-mce-fragment=\"1\"\u003eThe FireGene 1st strand cDNA Synthesis Kit contains all of the reagents necessary for synthesis of full-length first-strand cDNA from total or poly A\u003c\/span\u003e\u003csup data-mce-fragment=\"1\"\u003e+\u003c\/sup\u003e\u003cspan data-mce-fragment=\"1\"\u003e RNA, including both oligo(dT) and random hexamers primers. This cDNA synthesis kit also works well with other cDNA-dependent techniques, including RT-PCR, cDNA probe preparation, quantitative PCR (qPCR), and PCR based cloning. \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eThe FireGene 1st strand cDNA Synthesis Kit is ideal for reverse transcription of many different RNA templates, including GC-rich templates and RNAs with high levels of secondary structure.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan data-mce-fragment=\"1\"\u003eThis kit includes 4 x gDNA wiper Mix that can completely remove residual genomic DNA contamination and ensure more reliable subsequent quantitative results, 2 x RT Mix that contains optimized buffer system and dNTP, and the Enzyme Mix contains FireGene Reverse Transcriptase and RNasin. You may also choose Oligo (dT)23VN, Random hexamers, or gene-specific primers as reverse transcription primers based on your requirements.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch4\u003eApplications\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eFirst-strand cDNA synthesis\u003c\/li\u003e\n\u003cli\u003ecDNA probe preparation\u003c\/li\u003e\n\u003cli\u003eRT-PCR\u003c\/li\u003e\n\u003cli\u003eSynthesis of cDNA libraries with a high proportion of full-length cDNAs\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/h4\u003e\n\u003ctable style=\"width: 100%;\" data-mce-style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 60%;\" data-mce-style=\"width: 60%;\"\u003e\u003cstrong\u003eComponent Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%;\" data-mce-style=\"width: 20%;\"\u003e\u003cstrong\u003eAmount\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%;\" data-mce-style=\"width: 20%;\"\u003e\u003cstrong\u003eStorage (°C)\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003e\n\u003cspan\u003eRNase free ddH\u003c\/span\u003e\u003csub\u003e2\u003c\/sub\u003e\u003cspan\u003eO\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e1 ml x 2\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003e\n\u003cspan\u003e2 x RT Mix\u003c\/span\u003e\u003csup\u003e*1\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e1 ml\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003e\n\u003cspan\u003eEnzyme Mix\u003c\/span\u003e\u003csup\u003e*2\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e200 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003e\u003cspan\u003eOligo (dT)\u003csub\u003e23\u003c\/sub\u003eVN (50 μM)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e100 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003e\u003cspan\u003eRandom hexamers (50 ng\/μl)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e100 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\"\u003e\u003cspan\u003e4 x gDNA wiper Mix\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e\u003cspan\u003e40 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e*1. Each contains 1 mM dNTP\u003c\/p\u003e\n\u003cp\u003e*2. Contains RNasin\u003c\/p\u003e\n\u003ch4\u003eRestrictions\u003cbr\u003e\n\u003c\/h4\u003e\n\u003cp\u003e\u003cspan\u003eFor Research or Educational Use Only.\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42896209936596,"sku":"FG-R00112","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FG0431-Main.jpg?v=1656608484"},{"product_id":"firegene-rt-mix-for-qpcr-gdna-wiper","title":"FireGene RT mix for qPCR (+gDNA wiper)","description":"\u003cp data-mce-fragment=\"1\"\u003e\u003cspan\u003eThe FireGene RT mix for qPCR (gDNA wiper) is suitable for two-step qRT-PCR detection. It can react quickly by simply adding template RNA and water. The \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eincluded\u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\u003cspan\u003egDNA wiper Mix \u003c\/span\u003e\u003cspan\u003ecan completely remove the residual genomic DNA contamination and ensure more reliable subsequent quantification results. The T Mix contains all the components required for the reverse transcription reaction, and stops the gDNA wiper function at the same time to ensure the \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003ecompleteness of \u003c\/span\u003e\u003cspan\u003ecDNA. This product is \u003cspan style=\"font-size: 14px;\" data-mce-style=\"font-size: 14px;\"\u003especifically\u003c\/span\u003e optimized for qPCR. The ratio-optimized Random primers\/Oligo dT primer mix enables cDNA synthesis to start from each region of the RNA transcript with the same reverse transcription efficiency, ensuring the reliability and repeatability of qPCR results. The reverse transcription product is compatible with SYBR® Green and probe-based qPCR. According to the purpose of the experiment, the corresponding reagents can be selected for high-performance gene expression analysis.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch4 data-mce-fragment=\"1\"\u003eApplications\u003c\/h4\u003e\n\u003cul data-mce-fragment=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003eFirst-strand cDNA synthesis\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003ecDNA probe preparation\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003eRT-PCR\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003eSynthesis of cDNA libraries with a high proportion of full-length cDNAs\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4 data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eComponents\u003c\/span\u003e\u003c\/h4\u003e\n\u003ctable style=\"width: 100%;\" data-mce-style=\"width: 100%;\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 60%;\" data-mce-style=\"width: 60%;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eComponent Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%;\" data-mce-style=\"width: 20%;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eAmount\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%;\" data-mce-style=\"width: 20%;\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage (°C)\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\" data-mce-fragment=\"1\"\u003e\n\u003cspan data-mce-fragment=\"1\"\u003eRNase free ddH\u003c\/span\u003e\u003csub data-mce-fragment=\"1\"\u003e2\u003c\/sub\u003e\u003cspan data-mce-fragment=\"1\"\u003eO\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\" data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e1 ml x 2\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\" data-mce-fragment=\"1\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\" data-mce-fragment=\"1\"\u003e\n\u003cspan data-mce-fragment=\"1\"\u003e5 x RT Mix\u003c\/span\u003e\u003csup data-mce-fragment=\"1\"\u003e*1\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\" data-mce-fragment=\"1\"\u003e\u003cspan\u003e400 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\" data-mce-fragment=\"1\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\" data-mce-fragment=\"1\"\u003e\n\u003cspan data-mce-fragment=\"1\"\u003eControl No RT Mix\u003c\/span\u003e\u003csup data-mce-fragment=\"1\"\u003e*2\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\" data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e40 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\" data-mce-fragment=\"1\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd style=\"width: 50%;\" data-mce-style=\"width: 50%;\" data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e4 x gDNA wiper Mix\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\" data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e400 μl\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 25%;\" data-mce-style=\"width: 25%;\" data-mce-fragment=\"1\"\u003e-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e*1. Contains FireGene Reverse Transcriptase, dNTP, RNasin, Random primers\/Oligo dT primer mix.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e*2. The ingredients are the same as 5 x RT Mix, except that FireGene Reverse Transcriptase is not included.\u003c\/p\u003e\n\u003ch4 data-mce-fragment=\"1\"\u003eRestrictions\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/h4\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eFor Research or Educational Use Only.\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42896340058324,"sku":"FG-R00113","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder_d43c590f-fb9c-4544-87ad-b6fec046b56b.jpg?v=1656611950"},{"product_id":"magicscript-ii-first-strand-cdna-synthesis-kit","title":"MagicScript II First Strand cDNA Synthesis Kit","description":"\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eMagicScript II cDNA First Strand Synthesis Kit features two optimized mixes, MagicScript II Enzyme Mix and MagicScript II Reaction Mix. The enzyme mix combines MagicScript II Reverse Transcriptase and RNase Inhibitor, while the reaction mix contains an optimized buffer. MagicScript II Reverse Transcriptase is a recombinant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability. It can be used to synthesize first strand cDNA at higher temperatures than the wild type M-MuLV. The enzyme is active up to 50°C, providing higher specificity and higher yield of cDNA.\n             \n             The kit also provides two optimized primers for reverse transcription and nuclease-free water. An anchored Oligo-dT primer [d(T)23VN] forces the primer to anneal to the beginning of the polyA tail. The optimized Random Primer Mix provides random and consistent priming sites covering the entire RNA templates including both mRNAs and non-polyadenylated RNAs. The first strand cDNA product generated is up to 10 kb.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003ecDNA Synthesis; RT-PCR \u0026amp; cDNA Synthesis; Reverse Transcription (cDNA Synthesis)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e50 rxn \/ 100 rxn\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e* MagicScript II Reaction Mix (2X)\n             \n             * MagicScript II Enzyme Mix (10X)\n             \n             * Oligo d(T)23VN (50 uM)\n             \n             * dNTPs (10 mM)\n             \n             * Nuclease-free H2O\n             \n             * Random Primer Mix (60 uM)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eFor Research Use Only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eStore the MagicScript II First Strand cDNA Synthesis Kit at -20°C. Please avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003ch4\u003eRestrictions\u003c\/h4\u003e\n\u003cp\u003e\u003cspan\u003eFor Research Use Only.\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42990250557652,"sku":"FG-ENY101","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder_a2bdb91f-0bc3-4672-98ae-70a0ca759270.jpg?v=1657059522"},{"product_id":"magicscript-ii-rt-master-mix-for-qpcr","title":"MagicScript II RT Master Mix for qPCR","description":"\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eMagicScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) with reduced RNase H activity and increased thermal stability compared to wild-type MMLV RT. MagicScript II RT Master Mix for qPCR is a reverse transcription detection reagent suitable for two-step RT-qPCR. The 5X MagicScript II RT Mix contains all the reagents required for Reverse transcription reactions [MagicScript II Reverse Transcriptase, dNTP, RNase Inhibitor, Random primers\/Oligo (dT)23VN Primer Mix and optimized reaction solution]. The volume of RNA template can be up to 80% of the total volume, which is very suitable for the reverse transcription reaction of low concentration RNA template. 5 x MagicScript II RT Mix will not freeze at -20°C storage, which is very convenient to use.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003ecDNA Synthesis; RT-PCR \u0026amp; cDNA Synthesis; Reverse Transcription (cDNA Synthesis)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e10 rxn \/ 100 rxn\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e5x\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eNuclease-free H2O; 5X MagicScript II RT Mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eFor Research Use Only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eStore the MagicScript II RT Master Mix for qPCR at -20°C. Please avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42990250623188,"sku":"FG-ENY102","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder_75183d25-c3be-4d8f-b6ba-070f5cef0cce.jpg?v=1657059523"},{"product_id":"magicscript-ii-reverse-transcriptase","title":"MagicScript II Reverse Transcriptase","description":"\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eMagicScript II Reverse Transcriptase is a recombinant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability. It can be used to synthesize first strand cDNA at higher temperatures than the wild type M-MuLV. The enzyme is active up to 48°C, providing higher specificity, higher yield of cDNA and more full-length cDNA product up to 12 kb.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eThe gene encoding a mutant M-MuLV Reverse Transcriptase (RNase H-) is expressed in E. coli and purified to near homogeneity.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003ecDNA Synthesis, RT-qPCR, RT-PCR and cDNA Synthesis , PCR, Reverse Transcription (cDNA Synthesis), RT-PCR \u0026amp; cDNA Synthesis\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e4000 U \/ 10000 U\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003e200,000 U\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eMagicScript II Reverse Transcriptase (200,000 U\/ml); 100 mM DTT (10X); 5X First-Strand Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eOne unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-insoluble material in a total reaction volume of 50 μl in 10 minutes at 37°C using poly(rA)•oligo(dT)18 as template.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eUsage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eFor Research Use Only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30%;\" data-mce-style=\"width: 30%;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 70%;\" data-mce-style=\"width: 70%;\"\u003eStorage Conditions: 20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, 0.01% IGEPAL CA-630, pH 7.5 @ 25°C. Store the MagicScript II Reverse Transcriptase at -20°C. Please avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":42990251835604,"sku":"FG-ENY125","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/products\/FireGene-logo-01-placeholder_a760d881-822d-46eb-a859-0d4e532a3989.jpg?v=1657059556"}],"url":"https:\/\/firegene.com\/collections\/reverse-transcription-and-cdna-synthesis\/other-reagents.oembed","provider":"FireGene","version":"1.0","type":"link"}