{"title":"Single-Cell Suspension Cleanup Workflow","description":"\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003cstrong\u003eSingle-Cell Suspension Cleanup Workflow\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003eDissociation gets the cells out of the tissue. Cleanup determines what actually goes into the cartridge.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003eA suspension with 30% dead cells isn't just a viability problem — dead cells release RNA that contaminates ambient profiles, inflate doublet rates, and generate artifactual clusters that take hours of bioinformatics to filter out. Debris raises background signal. Red blood cells consume capture sites on the 10x bead without contributing any usable transcriptome. The cleanup step before loading is where these problems are solved, not downstream.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003eThis collection covers the post-dissociation cleanup reagents in FireGene's single-cell preparation workflow, organized by the contaminant being removed.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003ca href=\"https:\/\/firegene.com\/products\/dead-cell-debris-removal-kit-fg-ba3339\"\u003e\u003cstrong\u003eDead Cell and Debris Removal\u003c\/strong\u003e\u003c\/a\u003e The Dead Cell Debris \u0026amp; Impurities Removal Kit (FG-BA3339) removes apoptotic cells, necrotic cells, and cellular debris while preserving live cell integrity, viability, and surface antigen expression — the surface markers that flow cytometry and immunophenotyping downstream applications depend on. Operates via density gradient centrifugation: low-density dead cells and debris float to the upper layer while viable intact cells sink, yielding a clean, enriched single-cell suspension. \u003cspan class=\"inline-flex\" data-state=\"closed\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003ca href=\"https:\/\/firegene.com\/products\/digestive-tissue-cell-debris-removal-kit\"\u003e\u003cstrong\u003eDigestive Tissue Debris Removal\u003c\/strong\u003e\u003c\/a\u003e The Digestive Tissue Cell Debris Removal Kit is designed specifically for single-cell suspensions from gastrointestinal tissues, where mucus-associated material, epithelial debris, and dissociation byproducts require a tissue-specific layered separation workflow. \u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003ca href=\"https:\/\/firegene.com\/products\/red-blood-cell-lysis-kit-fg-ba3311\"\u003e\u003cstrong\u003eRed Blood Cell Lysis\u003c\/strong\u003e\u003c\/a\u003e The Red Blood Cell Lysis Kit uses osmotic shock and enzymatic digestion to selectively lyse erythrocytes from single-cell suspensions — removing the cellular noise caused by RBCs in blood-contaminated tissue preparations and blood-derived samples. \u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003eCompatible with 10x Genomics Chromium, BD Rhapsody, and Drop-seq workflows.\u003c\/p\u003e","products":[{"product_id":"red-blood-cell-lysis-kit-fg-ba3311","title":"FireGene Red Blood Cell Lysis Kit - Enriched Single-Cell Prep","description":"\u003ch3 id=\"overview\"\u003eOverview\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Red Blood Cell Lysis Kit\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eis formulated for the rapid and effective removal of red blood cells (RBCs) from single-cell suspensions. Widely applied in flow cytometry, immunological studies, and molecular biology, this kit improves the clarity and quality of samples used for downstream cell analysis and extraction workflows.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch3 id=\"background-information\"\u003eBackground Information\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eCrucial for\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eprocessing peripheral blood and lymphoid tissues\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eEnhances accuracy in:\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry\u003c\/strong\u003e, by isolating white blood cells for phenotypic analysis.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSingle-cell sequencing\u003c\/strong\u003e, by removing cellular noise caused by erythrocytes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProtein and nucleic acid extraction\u003c\/strong\u003e, by providing purified cellular material.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003eWidely used in\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eclinical diagnostics, immunological research\u003c\/strong\u003e, and\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ebiomarker discovery\u003c\/strong\u003e.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch3 id=\"detection-principle\"\u003eDetection Principle\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eCombines\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eosmotic shock and enzymatic digestion\u003c\/strong\u003e:\n\u003cul\u003e\n\u003cli\u003eThe buffer induces a\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ehypertonic environment\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003ethat causes red blood cells to absorb water and rupture.\u003c\/li\u003e\n\u003cli\u003eEnzymes degrade\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eRBC-specific membrane components\u003c\/strong\u003e, ensuring selective lysis.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003eResult:\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWhite blood cells and other cell types remain intact\u003c\/strong\u003e, suitable for further analysis or functional assays.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eSpecifications\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eSingle-cell sequencing, cell culture or other cell-related detections\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eCompatible Sample Types\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eSingle-cell suspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eSupported Instruments\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eWater bath, horizontal centrifuge, cell counter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e4 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eShelf-life\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eKit Components\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e100 mL\/Kit\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 60%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e100 mL\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003eRed Blood Cell Lysis Solution\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e500 mL\/Kit\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40.0716%; font-weight: bold; padding: 8px; border: 1px solid rgb(221, 221, 221);\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 59.9284%; font-weight: bold; padding: 8px; border: 1px solid rgb(221, 221, 221);\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e500 mL\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 40.0716%;\"\u003eRed Blood Cell Lysis Solution\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 59.9284%;\"\u003e500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eProduct FAQ\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e1.    Q: Is this lysis buffer only suitable for removing red blood cells after vascular tissue dissociation? Is it effective for red blood cells in samples from other sources (e.g., blood, spleen, tumor tissue)? Can it be used for non-mammalian samples (e.g., avian blood)?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The lysis buffer is suitable for red blood cell removal from various sample sources, including vascular tissue dissociation suspensions, peripheral blood, spleen homogenate suspensions, and tumor tissue dissociation solutions. It primarily ruptures red blood cells through osmotic pressure differences, featuring strong versatility. However, it is not suitable for non-mammalian samples. Non-mammalian red blood cells (e.g., avian) contain nuclei and have significantly different cell membrane structures from mammals. This lysis buffer cannot effectively rupture their cell membranes, so a dedicated avian red blood cell lysis buffer must be used. For mammalian samples from different sources, only the lysis time needs to be adjusted based on red blood cell content, with no other operational changes required.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e2.    Q: The instruction manual requires mixing the single-cell suspension with the lysis buffer at a 1:3 ratio. If the sample has extremely low red blood cell content (e.g., purified endothelial cell suspension) or extremely high content (e.g., whole blood sample), does the ratio need to be adjusted? Should the lysis time be changed accordingly after adjustment?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The ratio should be flexibly adjusted based on red blood cell content: ① For extremely low red blood cell content (e.g., endothelial cell suspension with red blood cell ratio \u0026lt;5%), the ratio can be adjusted to 1:1 (1 mL suspension + 1 mL lysis buffer) to avoid excessive lysis buffer damaging target cells, with lysis time shortened to 1-3 minutes; ② For extremely high red blood cell content (e.g., whole blood sample), the ratio should be increased to 1:5 (1 mL whole blood + 5 mL lysis buffer) to ensure red blood cells fully contact the lysis buffer, with lysis time extended to 10-15 minutes and gentle pipetting every 3 minutes to prevent incomplete lysis. After adjusting the ratio, the lysis time must be adapted accordingly, with the core criterion for stopping lysis being a clear and transparent solution.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e3.    Q: Step 3 requires \"red blood cell lysis on wet ice\". What is the difference between wet ice and regular ice? What impact will lysis on regular ice (non-wet ice) or at room temperature have on experimental results?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Wet ice refers to a mixture of ice cubes and a small amount of water, which allows the centrifuge tube to evenly contact the low-temperature environment and maintain a stable lysis temperature of approximately 4°C. Regular ice (dry ice or block ice) easily causes uneven or excessively low local temperatures in the centrifuge tube, affecting lysis efficiency. Lysis on regular ice may result in uneven lysis (over-lysis in some areas and incomplete lysis in others). Lysis at room temperature (25-30°C) significantly enhances the activity of the lysis buffer, which not only rapidly ruptures red blood cells but also damages target cells (e.g., vascular endothelial cells, immune cells), reducing target cell viability by over 30% and even causing complete cell death in severe cases. Lysis must be strictly performed on wet ice.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e4.    Q: Step 4 requires \"adding an equal volume of PBS containing 5% FBS to stop lysis\". Does \"equal volume\" refer to the volume of the single-cell suspension or the post-lysis mixture? What consequences will occur if PBS without FBS is used to stop lysis?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: \"Equal volume\" refers to the volume of the post-lysis mixture (suspension + lysis buffer). For example, 1 mL suspension + 3 mL lysis buffer forms a 4 mL mixture, so 4 mL of PBS containing 5% FBS should be added to stop lysis. Using PBS without FBS cannot neutralize the activity of residual lysis buffer, which will continue to act on target cells, damaging their cell membranes. This leads to a 25%-40% decrease in cell viability after subsequent centrifugation and increases cell debris. The protein components in FBS quickly block the active sites of the lysis buffer and protect cells, making it an indispensable component for stopping lysis.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e5.    Q: The centrifugation parameters require \"4°C, 450×g for 5 minutes\". If only a vertical centrifuge is available in the laboratory or if parameters are set incorrectly (e.g., excessively high\/low speed), what impact will this have? Can room-temperature centrifugation replace 4°C centrifugation?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: A vertical centrifuge cannot replace a horizontal centrifuge. The centrifugal force direction of a vertical centrifuge is perpendicular to the centrifuge tube, resulting in uneven cell pelleting (loose pellets or wall adhesion), which increases the risk of aspirating target cells when discarding the supernatant. A horizontal centrifuge ensures uniform cell pelleting at the tube bottom, facilitating subsequent operations. Impacts of incorrect parameters: ① Excessively high speed (e.g., 800×g) compacts the cell pellet, making resuspension difficult and potentially crushing fragile cells (e.g., lymphocytes); ② Excessively low speed (e.g., 200×g) prevents full cell pelleting, leading to target cell loss with the supernatant. Room-temperature centrifugation cannot replace 4°C centrifugation, as room temperature accelerates cell metabolism and enhances residual lysis buffer activity, causing further cell damage. Strict adherence to 4°C centrifugation is recommended.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e6.    Q: Steps 6 and 7 require \"two washing cycles\". What impact will omitting one cycle (only one wash) have on subsequent experiments? Is it feasible to replace PBS containing 5% FBS with RPMI 1640 medium for washing?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Omitting one wash cycle fails to completely remove residual lysis buffer and red blood cell debris: ① Residual lysis buffer interferes with subsequent experiments (e.g., cell capture in single-cell sequencing, fluorescence signal detection in flow cytometry), leading to data bias; ② Excessive red blood cell debris affects cell counting accuracy and may clog detection instruments (e.g., cell counter channels). RPMI 1640 medium can replace PBS containing 5% FBS for washing—both liquids rinse residual impurities, and RPMI 1640 also provides nutrients for cells. However, ensure the medium contains no antibiotics or other components that may affect subsequent experiments. For protein extraction, both liquids are suitable; for cell culture, PBS containing 5% FBS is preferred to better maintain cell status.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e7.    Q: After lysis, the solution fails to become \"clear and transparent\" and remains turbid or has red precipitates. What are the possible causes and how to address them?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Common causes and solutions for incomplete clarity: ① Insufficient lysis time (red blood cells not fully ruptured): Add a small amount of lysis buffer (1\/3 of the original ratio), continue incubation on wet ice for 3-5 minutes, and pipette gently every 2 minutes until the solution clears; ② Excessive impurities (e.g., tissue debris, fibrous components) causing turbidity: First filter the sample through a 70μm cell sieve to remove impurities, then check the solution status; ③ Uneven mixing of lysis buffer (insufficient concentration in some areas): Pipette the mixture gently 10-15 times to ensure uniform distribution of the lysis buffer. If no improvement is observed, add an equal volume of lysis buffer and extend incubation time.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e8.    Q: The product requires \"storage at 4°C in the dark\". If the ice pack melts during transportation and the product is left at room temperature (25°C) for 1 hour, can it still be used? What consequences will occur if stored at room temperature for a long time?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The product can still be used after 1 hour at room temperature, but it must be immediately returned to 4°C and fully mixed before use. The osmotic balance system of the lysis buffer remains stable during short-term room-temperature storage (≤1 hour), with ≤5% activity loss, which does not affect lysis efficiency. Long-term room-temperature storage (over 24 hours) causes changes in the salt ion concentration of the lysis buffer, leading to osmotic imbalance. This not only fails to effectively rupture red blood cells but also causes target cell dehydration or swelling. Additionally, it promotes microbial growth (even though the product is sterile, microbes multiply easily at room temperature), resulting in sample contamination. Therefore, strict storage at 4°C in the dark is mandatory, and repeated removal to room temperature should be avoided.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cstrong\u003e\u003cspan\u003e9.    Q: Step 8 requires \"resuspending with the minimum possible volume of PBS containing 5% FBS\". How to determine the specific volume? If the resuspension volume is too large (resulting in low cell concentration), how to concentrate the cells subsequently?\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The resuspension volume depends on subsequent experimental needs: ① For flow cytometry or single-cell sequencing, resuspend at a concentration of 1×10⁶-1×10⁷ cells\/mL (e.g., 1-2 mL PBS for a pellet containing approximately 1×10⁷ cells); ② For protein extraction, adjust based on the requirements of the extraction kit (typically 50-100 μL). If the volume is too large (low concentration), concentrate by centrifuging at 450×g for 5 minutes at 4°C, discard part of the supernatant, and re-adjust the concentration with a small volume of PBS. Operate gently during centrifugation to avoid cell damage, and limit concentration cycles to ≤2 to prevent viability loss.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e10.    Q: Compared with other brands of red blood cell lysis buffers, this product is labeled \"gentle removal\". What specific features reflect this? What is the approximate damage rate to sensitive cells (e.g., hematopoietic stem cells, nerve cells)?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: \"Gentle removal\" is reflected in two key aspects: ① A mild osmotic gradient design that targets only the osmotic sensitivity of red blood cell membranes, minimizing damage to target cell membranes; ② No irritating components (e.g., proteases) are added, avoiding damage to cell surface proteins and internal structures. The damage rate to sensitive cells is low: Experimental data shows that after treating hematopoietic stem cell suspensions with this product, cell viability remains above 85% (damage rate \u0026lt;15%); for nerve cell suspensions, viability remains above 80% (damage rate \u0026lt;20%), significantly lower than ordinary lysis buffers (which typically cause \u0026gt;30% damage to sensitive cells). For experiments involving sensitive cells, control lysis time to 1-5 minutes to reduce cell exposure to the lysis buffer and further lower the damage rate.\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"100 ml\/kit","offer_id":46299527479508,"sku":"FG-BA3311-100","price":38.0,"currency_code":"USD","in_stock":true},{"title":"500 ml\/kit","offer_id":47718719520980,"sku":"FG-BA3311-500","price":168.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/files\/BA3311_be66e0c8-e409-43c9-bac3-0a951e0a0a74.png?v=1774420035"},{"product_id":"dead-cell-debris-removal-kit-fg-ba3339","title":"FireGene Dead Cell Debris \u0026 Impurities Removal Kit - Purified Cell Suspensions","description":"\u003cp\u003e\u003cstrong\u003eFireGene Dead Cell Debris and Impurities Removal Kit\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eis designed to purify single-cell suspensions by removing dead cells, apoptotic bodies, and unwanted debris. This enhances the clarity, viability, and analytical accuracy of your cell samples, especially in applications like flow cytometry, single-cell sequencing, and immunocytochemistry.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch3 id=\"background-information\"\u003eBackground Information\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eUsed extensively to improve\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003esample quality for downstream applications\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eBenefits include:\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImproved cell sorting and gating accuracy\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003ein flow cytometry.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEnhanced data quality\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003ein scRNA-seq and proteomics.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIncreased reliability\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eof results in cell culture by enriching live cell populations.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003eValuable in\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eimmunocytochemistry\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eand\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003estaining assays\u003c\/strong\u003e, where background noise from dead cells must be minimized.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch3 id=\"detection-principle\"\u003eDetection Principle\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eOperates via\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003edensity gradient centrifugation\u003c\/strong\u003e:\n\u003cul\u003e\n\u003cli\u003eCell suspensions are layered and spun under controlled conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLow-density dead cells and debris\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003efloat in the upper layer.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eViable, intact cells\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003esink to the bottom.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003eFinal output:\n\u003cul\u003e\n\u003cli\u003eA\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eclean, enriched single-cell suspension\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eoptimized for high-precision biological research.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eSpecifications\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eSingle-cell sequencing, cell culture or other cell-related detections\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eCompatible Sample Types\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eSingle-cell suspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eSupported Instruments\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eWater bath, horizontal centrifuge, cell counter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e4 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eShelf-life\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eKit Components\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e10 reactions\/kit\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable height=\"150\" style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px; height: 150px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003cth style=\"width: 57.0662%; padding: 8px; border: 1px solid rgb(221, 221, 221); background: rgb(240, 240, 240); height: 19.5938px; text-align: left;\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"width: 42.9338%; padding: 8px; border: 1px solid rgb(221, 221, 221); background: rgb(240, 240, 240); height: 19.5938px;\"\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 39.2031px;\"\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 57.0662%; height: 39.2031px;\"\u003e\u003cspan\u003eDRS 1 (Dead cell debris and impurities removal solution 1)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 42.9338%; height: 39.2031px;\"\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.2031px;\"\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 57.0662%; height: 39.2031px;\"\u003e\u003cspan\u003eDRS 2 (Dead cell debris and impurities removal solution 2)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 42.9338%; height: 39.2031px;\"\u003e20 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e50 reactions\/kit\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"width: 57.0662%; padding: 8px; border: 1px solid rgb(221, 221, 221); background: rgb(240, 240, 240);\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"width: 42.9338%; padding: 8px; border: 1px solid rgb(221, 221, 221); background: rgb(240, 240, 240);\"\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 57.0662%;\"\u003e\u003cspan\u003eDRS 1 (Dead cell debris and impurities removal solution 1)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 42.9338%;\"\u003e50 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 57.0662%;\"\u003e\u003cspan\u003eDRS 2 (Dead cell debris and impurities removal solution 2)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 42.9338%;\"\u003e100 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3 id=\"detection-principle\"\u003eProduct FAQ\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eQ: What is the purpose of a dead cell debris removal kit?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-end=\"353\" data-start=\"175\"\u003eA: A dead cell debris removal kit is designed to eliminate apoptotic cells, cell fragments, and impurities from cell suspensions, resulting in a cleaner and more homogeneous sample.\u003c\/p\u003e\n\u003cp data-end=\"838\" data-start=\"355\"\u003eThis step is essential because contaminated samples can significantly compromise downstream experimental accuracy and reproducibility. As explained in this \u003ca href=\"https:\/\/firegene.com\/blogs\/knowledge-center\/firegene-dead-cell-debris-impurities-removal-kit-enhance-single-cell-sample-quality-and-viability\"\u003edead cell debris removal guide\u003c\/a\u003e\u003ca href=\"https:\/\/firegene.com\/blogs\/news\/clean-cells-clear-data-an-optimized-method-for-removing-dead-cells-and-debris-in-advanced-research-workflows?utm_source=chatgpt.com\" class=\"decorated-link\" rel=\"noopener\" data-end=\"708\" data-start=\"511\"\u003e, \u003c\/a\u003epoor sample quality is one of the most common bottlenecks in modern biological research.\u003c\/p\u003e\n\u003cp data-end=\"253\" data-start=\"170\" data-section-id=\"3sy1a4\"\u003e\u003cstrong\u003eQ: Why are dead cells and debris considered a hidden threat in cell suspensions?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-end=\"371\" data-start=\"255\"\u003eA: Dead cells and cellular debris are often overlooked, but they can significantly interfere with experimental systems.\u003c\/p\u003e\n\u003cp data-end=\"687\" data-start=\"373\"\u003eThey release degraded RNA, enzymes, and intracellular components that contaminate viable cells, ultimately reducing data accuracy. As highlighted in this\u003cbr data-end=\"529\" data-start=\"526\"\u003e\u003ca href=\"https:\/\/firegene.com\/blogs\/knowledge-center\/the-invisible-killer-in-your-suspension-your-experimental-data-is-being-silently-devoured-by-dead-cell-debris\"\u003e👉 https:\/\/firegene.com\/blogs\/knowledge-center\/the-invisible-killer-in-your-suspension-your-experimental-data-is-being-silently-devoured-by-dead-cell-debris\u003c\/a\u003e\u003c\/p\u003e\n\u003cp data-end=\"841\" data-start=\"689\"\u003ethese contaminants act as an “invisible killer,” silently compromising experimental outcomes and data integrity.\u003c\/p\u003e\n\u003cp data-section-id=\"10k5cte\" data-start=\"242\" data-end=\"309\"\u003e\u003cstrong\u003eQ: Why is sample quality critical in modern cell-based research?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-start=\"311\" data-end=\"468\"\u003eA: In advanced workflows such as single-cell RNA sequencing, flow cytometry, and primary cell culture, \u003cstrong data-start=\"411\" data-end=\"467\"\u003esample quality is the foundation of reliable results\u003c\/strong\u003e.\u003c\/p\u003e\n\u003cp data-start=\"470\" data-end=\"799\"\u003eAfter cell isolation, samples often contain dead cells, membrane fragments, and impurities that can significantly compromise downstream performance. As explained in this\u003cbr data-start=\"639\" data-end=\"642\"\u003e👉 \u003ca data-start=\"645\" data-end=\"797\" rel=\"noopener\" class=\"decorated-link\" href=\"https:\/\/firegene.com\/blogs\/knowledge-center\/clean-cells-clear-data-an-optimized-method-for-removing-dead-cells-and-debris-in-advanced-research-workflows\"\u003ehttps:\/\/firegene.com\/blogs\/knowledge-center\/clean-cells-clear-data-an-optimized-method-for-removing-dead-cells-and-debris-in-advanced-research-workflows\u003cspan aria-hidden=\"true\" class=\"ms-0.5 inline-block align-middle leading-none\"\u003e\u003csvg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"20\" height=\"20\" aria-hidden=\"true\" data-rtl-flip=\"\" class=\"block h-[0.75em] w-[0.75em] stroke-current stroke-[0.75]\"\u003e\u003cuse href=\"\/cdn\/assets\/sprites-core-fxfgsfqf.svg#304883\" fill=\"currentColor\"\u003e\u003c\/use\u003e\u003c\/svg\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp data-start=\"801\" data-end=\"929\"\u003epoor sample quality remains a major bottleneck even in highly advanced research systems.\u003c\/p\u003e\n\u003cp data-section-id=\"zdeluq\" data-start=\"87\" data-end=\"179\"\u003e\u003cspan role=\"text\"\u003e\u003cstrong data-start=\"91\" data-end=\"179\"\u003eQ: How can I improve cell viability and data quality in single-cell experiments?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-start=\"181\" data-end=\"473\"\u003eA: Achieving high cell viability and reliable sequencing data largely depends on optimized sample preparation, especially during tissue dissociation and debris removal. Poor handling can lead to cell damage, RNA degradation, and biased cell populations—ultimately affecting downstream results.\u003c\/p\u003e\n\u003cp data-start=\"475\" data-end=\"942\"\u003eUsing specialized kits designed for gentle dissociation and cleanup can significantly enhance outcomes. As highlighted in this article:\u003cbr data-start=\"610\" data-end=\"613\"\u003e👉 \u003ca data-start=\"616\" data-end=\"775\" rel=\"noopener\" class=\"decorated-link\" href=\"https:\/\/firegene.com\/collections\/sample-preparation-kit?utm_source=chatgpt.com\"\u003eSay Goodbye to Experimental Hurdles, Unlock High-Quality Single-Cell Research\u003cspan aria-hidden=\"true\" class=\"ms-0.5 inline-block align-middle leading-none\"\u003e\u003csvg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"20\" height=\"20\" aria-hidden=\"true\" data-rtl-flip=\"\" class=\"block h-[0.75em] w-[0.75em] stroke-current stroke-[0.75]\"\u003e\u003cuse fill=\"currentColor\" href=\"\/cdn\/assets\/sprites-core-fxfgsfqf.svg#304883\"\u003e\u003c\/use\u003e\u003c\/svg\u003e\u003c\/span\u003e\u003c\/a\u003e\u003cbr data-start=\"775\" data-end=\"778\"\u003ehigh-quality workflows focus on maximizing cell viability, RNA integrity, and reproducibility across different sample types.\u003c\/p\u003e\n\u003cdiv class=\"type-page\"\u003e\n\u003cdiv class=\"product-content\"\u003e\n\u003cdiv class=\"con-wrap\"\u003e\n\u003cdiv class=\"jianjie tab selected\"\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eQ: When layering 2mL of the mixed solution (cell suspension + DRS①) on top of 2mL DRS② according to the procedure, the mixed solution quickly infiltrates into DRS②, and the two layers gradually merge, making it impossible to form a stable stratification. What causes this, and how to avoid infiltration?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: Infiltration is mostly due to unstable density difference between DRS① and DRS②, or the mixed solution containing a large amount of small-molecule impurities. Prevention methods: ① Before use, let DRS① and DRS② stand in a 4℃ refrigerator for 30 minutes to ensure uniform reagent density; ② Before layering, filter the mixed solution through a 20μm cell sieve once to remove impurities such as fragmented organelles smaller than 20μm; ③ When layering, use a 1mL low-adhesion pipette tip to slowly drop the mixed solution along the tube wall, with an interval of 2 seconds between each drop. Wait for the previous drop to fully spread on the surface of DRS② before adding the next drop. This can increase the stratification stability to 90%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eQ: After centrifugation, only 3 layers of solution are observed (cell debris layer, buffy coat layer, and pellet layer), missing the transition layer between DRS① and DRS②, and the proportion of dead cells in the buffy coat layer reaches 30%. What causes the missing stratification, and how to adjust the operation?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: Missing stratification is mostly due to excessively high centrifuge acceleration\/deceleration, which damages the reagent interface. Adjustment methods: ① Strictly set the centrifuge acceleration and deceleration to the \"medium\" gear (e.g., set to \"5\" for digital centrifuges) to avoid stratification damage caused by high-speed acceleration\/deceleration; ② Before centrifugation, gently drop 100μL of PBS containing 2% FBS on top of 2mL DRS② to form a buffer layer; ③ Add 0.5mL DRS① to the mixed solution supplementally, re-layer and centrifuge according to the procedure. This can make the 4-layer structure clearly visible, and reduce the proportion of dead cells in the buffy coat layer to below 15%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eQ: When processing single-cell suspensions of tumor tissue (with a dead cell rate \u0026gt; 40% and a large amount of necrotic debris), the thickness of the cell debris layer reaches 1.5mL after centrifugation, completely covering the buffy coat layer, making it impossible to locate the viable cell layer. How to accurately find and aspirate the buffy coat layer?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: For an excessively thick debris layer, positioning requires \"stepwise aspiration + microscopic observation\". Operation methods: ① Use a 1mL low-adhesion pipette tip to slowly aspirate the upper cell debris layer. After aspirating 200μL each time, take 10μL of the liquid and observe it under a microscope. Stop aspiration when uniformly distributed round viable cells appear in the field of view; ② At this time, the buffy coat layer is 2-3mm below the liquid surface. Insert the pipette tip into this area along the tube wall and slowly aspirate 800μL of liquid; ③ If the aspirated liquid still contains a small amount of debris, filter it through a 20μm cell sieve again. The purity of viable cells can reach more than 90%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eQ: In Step 6, after diluting to 10mL with PBS containing 5% FBS and centrifuging at 4℃, 450×g for 5 minutes, the cell pellet of the buffy coat layer is \"loose and flocculent\", and about 25% of the cells are lost with the supernatant when discarding the supernatant. How to obtain a dense pellet?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: The loose pellet is due to the negative charge on the surface of viable cells, which causes mutual repulsion and difficulty in aggregation. Solutions: ① Increase the centrifugation speed to 500×g and extend the centrifugation time to 8 minutes; ② When diluting, add 30μL of 1% polylysine (self-prepared, non-toxic to cells) to 10mL PBS to neutralize the surface charge of cells; ③ After centrifugation, retain 150μL of supernatant and gently pipette to mix the pellet with the supernatant to avoid the loss of small-volume cells. This can increase the pellet density by 65% and reduce the cell loss rate to below 10%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eQ: After opening the kit, DRS① is stored at 4℃ in the dark for 13 months. When used, it is found that the volume of the buffy coat layer is reduced from 800μL to 300μL, and the dead cell removal rate is reduced from 90% to 60%. Is the reagent invalid, and how to quickly verify it?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: The validity period of DRS① at 4℃ is 1 year, and the density-regulating components are prone to degradation after expiration. Verification methods: ① Mix 1mL DRS① with 1mL RPMI 1640 containing 2% FBS, layer it on top of 2mL DRS②, and centrifuge at 4℃, 1400×g for 20 minutes according to the manual parameters; ② If the volume of the buffy coat layer is \u0026lt; 500μL after centrifugation and the proportion of dead cells under the microscope is \u0026gt; 25%, it indicates that DRS① is invalid; ③ If it still has partial effectiveness, increase the dosage of DRS① to 1.5mL (1mL for conventional use), which can restore the volume of the buffy coat layer to 600μL. It can barely be used for basic experiments (not recommended for single-cell sequencing).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eQ: When processing single-cell suspensions of spleen containing a large number of red blood cells, the boundary between the buffy coat layer and the red blood cell layer (between the debris layer and the buffy coat layer) is blurred after centrifugation, and red blood cells are easily mixed in during aspiration. How to distinguish and separate the two layers?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: The blurred boundary is due to the similar density of red blood cells and viable cells, making red blood cells easy to aggregate with viable cells. Distinction methods: ① After centrifugation, place the centrifuge tube on ice and let it stand for 10 minutes. Red blood cells will settle slowly due to their slightly higher density, forming a clear interface with the buffy coat layer; ② Use a 100μL low-adhesion pipette tip to aspirate a small amount of liquid and observe it under a microscope. If it is mainly composed of round viable cells (no biconcave red blood cells), it is the buffy coat layer; ③ Start aspiration 2mm below the interface and only aspirate 600-800μL of liquid. This can reduce the mixing rate of red blood cells to below 10%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eQ: In Step 2, when resuspending the cell pellet with RPMI 1640 containing 2% FBS, cells are easily adherent to the inner wall of the centrifuge tube, resulting in insufficient cell concentration in the subsequent mixed solution. How to avoid cell adhesion?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: Cell adhesion is mostly due to the centrifuge tube not being treated for anti-adhesion. Prevention methods: ① Before use, soak the centrifuge tube in RPMI 1640 containing 2% FBS for 5 minutes, take it out and drain it (no rinsing required) to form an anti-adhesion coating on the tube wall; ② When resuspending, use a low-adhesion pipette tip to gently pipette along the tube wall to avoid direct impact of cells on the tube wall; ③ If cells have already adhered, gently scrape the tube wall with a sterile cell scraper to collect the adherent cells. This can increase the cell recovery rate to more than 85%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eQ: When using DMEM medium instead of RPMI 1640 to resuspend cells according to the manual, it is found that the buffy coat layer shifts above the pellet layer after centrifugation, and the viable cell recovery rate is only 50%. What causes this, and how to adjust the use of DMEM?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: The density of DMEM is higher than that of RPMI 1640, leading to abnormal density of the mixed solution and affecting stratification. Adjustment methods: ① Use serum-free DMEM instead of serum-containing DMEM to reduce the overall density of the medium; ② When resuspending, increase the cell concentration from the conventional 1×10⁶ cells\/mL to 2×10⁶ cells\/mL to compensate for the density difference; ③ Reduce the centrifugation speed from 1400×g to 1350×g to avoid viable cell sedimentation caused by excessively high density. This can restore the viable cell recovery rate to about 80%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eQ: In Step 5, when aspirating the buffy coat layer, a small amount of pellet (containing impurities and dead cells) is accidentally aspirated, resulting in black particles in the cell suspension. How to remove these particles without losing viable cells?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: The aspirated pellet particles need to be removed by \"gradient washing\". Operation methods: ① Add the aspirated buffy coat liquid to 5mL of PBS containing 5% FBS and mix gently; ② Centrifuge at 4℃, 200×g for 3 minutes. At this time, impurities and dead cell particles will settle to the bottom of the tube. Discard 4mL of the upper liquid; ③ Repeat washing 2 times, and finally resuspend with 2mL of PBS containing 5% FBS. This can remove more than 95% of the black particles, with a viable cell loss rate of \u0026lt; 5%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eQ: After centrifugation, the buffy coat layer is \"fragmented\" (non-continuous band shape), and viable cells are scattered in different layers, with a recovery rate of only 40%. What causes this, and how to avoid fragmentation of the buffy coat layer?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: The fragmentation of the buffy coat layer is mostly due to the mixed solution not spreading uniformly along the tube wall during layering, resulting in local high concentration. Prevention methods: ① Before layering, tilt the 15mL centrifuge tube at 45°, let the mixed solution flow down slowly along the inclined tube wall, and spread naturally into a uniform thin layer; ② If the mixed solution has high viscosity (e.g., containing a large number of adherent cells), first add 50μL DRS① for dilution before layering; ③ Handle the centrifuge tube gently after centrifugation to avoid buffy coat layer breakage caused by vibration. This can increase the continuity of the buffy coat layer to 85% and restore the recovery rate to more than 75%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"FireGene","offers":[{"title":"2 reactions\/Kit","offer_id":47833054904532,"sku":"FG-BA3339-2rxns","price":119.0,"currency_code":"USD","in_stock":true},{"title":"10 reactions\/Kit","offer_id":46321636081876,"sku":"FG-BA3339-10rxns","price":479.0,"currency_code":"USD","in_stock":true},{"title":"50 reactions\/Kit","offer_id":46321638113492,"sku":"FG-BA3339-50rxns","price":1679.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/files\/Dead-Cell-Debris-and-Impurities-Removal-Kit.png?v=1775035536"},{"product_id":"anti-clumping-agent-single-cell-suspensions","title":"FireGene Anti-Clumping Agent for Single-Cell Suspensions","description":"\u003cp\u003e\u003cstrong\u003eFireGene Anti-Clumping Agent\u003c\/strong\u003e is designed to reduce cell aggregates in single-cell suspension samples derived from dissociated animal tissues. The treatment helps maintain a low aggregation rate, supporting smoother downstream workflows such as flow cytometry analysis, single-cell sequencing, cell culture, and other cell-related assays. The protocol uses a short 30°C incubation followed by washing and resuspension, with cell concentration adjusted according to downstream requirements.\u003c\/p\u003e\n\u003ch2\u003eBackground Information\u003c\/h2\u003e\n\u003cp\u003eCell clumping can reduce sample quality in single-cell workflows by affecting counting, sorting, sequencing loading, and downstream assay consistency. An anti-clumping treatment helps improve suspension uniformity before analysis or culture.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Anti-Clumping Agent for Single-Cell Suspensions\u003c\/strong\u003e provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.\u003c\/p\u003e\n\u003ch3\u003eResearch Areas\u003c\/h3\u003e\n\u003cp\u003eThis product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSingle-cell sample preparation\u003c\/li\u003e\n\u003cli\u003eFlow cytometry assay development\u003c\/li\u003e\n\u003cli\u003eCell sorting workflows\u003c\/li\u003e\n\u003cli\u003ePrimary tissue dissociation workflows\u003c\/li\u003e\n\u003cli\u003eCell culture research\u003c\/li\u003e\n\u003cli\u003eCancer and immunology research\u003c\/li\u003e\n\u003cli\u003eDrug discovery assays\u003c\/li\u003e\n\u003cli\u003eTranslational biomedical research\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKey Applications\u003c\/h3\u003e\n\u003cp\u003eThe prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eReduction of cell aggregates\u003c\/li\u003e\n\u003cli\u003eSingle-cell suspension cleanup\u003c\/li\u003e\n\u003cli\u003eFlow cytometry analysis\u003c\/li\u003e\n\u003cli\u003eSingle-cell sequencing preparation\u003c\/li\u003e\n\u003cli\u003eCell culture workflows\u003c\/li\u003e\n\u003cli\u003eCell counting and quality control\u003c\/li\u003e\n\u003cli\u003ePost-dissociation sample processing\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eAnti Clumping Agent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBrand\u003c\/td\u003e\n\u003ctd\u003eFireGene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalog No.\u003c\/td\u003e\n\u003ctd\u003eFG-BA3345\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePack Size\u003c\/td\u003e\n\u003ctd\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Type\u003c\/td\u003e\n\u003ctd\u003eSingle-cell suspensions from dissociated animal tissues\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Function\u003c\/td\u003e\n\u003ctd\u003eReduction of cell aggregates\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWorkflow\u003c\/td\u003e\n\u003ctd\u003eCentrifugation, anti-clumping treatment, incubation, washing, resuspension, quality control\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDownstream Applications\u003c\/td\u003e\n\u003ctd\u003eFlow cytometry analysis, single-cell sequencing, cell culture, cell-related assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTreatment Conditions\u003c\/td\u003e\n\u003ctd\u003e30°C for 20 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended Cell Concentration During Treatment\u003c\/td\u003e\n\u003ctd\u003eNot more than 2,000 cells\/μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Instruments\u003c\/td\u003e\n\u003ctd\u003eWater bath, benchtop centrifuge, cell counter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Reagents\u003c\/td\u003e\n\u003ctd\u003ePBS, FBS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Consumables\u003c\/td\u003e\n\u003ctd\u003eLow-retention pipette tips, centrifuge tubes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOptional Related Reagent\u003c\/td\u003e\n\u003ctd\u003eRed Blood Cell Lysis Solution, Catalog No. BA3311\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e4°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf Life\u003c\/td\u003e\n\u003ctd\u003eOne year\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eResearch Use\u003c\/td\u003e\n\u003ctd\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eCatalog Number\u003c\/th\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAnti Clumping Agent\u003c\/td\u003e\n\u003ctd\u003eFG-BA3345\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"FireGene","offers":[{"title":"100 mL","offer_id":47868495724756,"sku":"FG-BA3345-100","price":299.0,"currency_code":"USD","in_stock":true},{"title":"500 mL","offer_id":47868495757524,"sku":"FG-BA3345-500","price":889.0,"currency_code":"USD","in_stock":true}]},{"product_id":"dead-cell-removal-solution","title":"FireGene Dead Cell Removal Solution for Single-Cell Suspension Cleanup","description":"\u003cp\u003e\u003cstrong\u003eFireGene Dead Cell Removal Solution\u003c\/strong\u003e is designed for removing dead cells from single-cell suspensions derived from dissociated mammalian tissues. The workflow helps reduce dead cell contamination and improve sample background for downstream experiments such as flow cytometry analysis, single-cell sequencing, cell culture, and related cell-based assays. The protocol uses a layered separation workflow with DCRS, followed by centrifugation, collection of the upper layer, washing, resuspension, and quality control.\u003c\/p\u003e\n\u003ch2\u003eBackground Information\u003c\/h2\u003e\n\u003cp\u003eDead cells can increase background, affect flow cytometry results, reduce single-cell sequencing quality, and interfere with downstream cell culture. A cleanup step after tissue dissociation helps prepare samples for more reliable cell-based analysis.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Dead Cell Removal Solution for Single-Cell Suspension Cleanup\u003c\/strong\u003e provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.\u003c\/p\u003e\n\u003ch3\u003eResearch Areas\u003c\/h3\u003e\n\u003cp\u003eThis product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSingle-cell sample preparation\u003c\/li\u003e\n\u003cli\u003eFlow cytometry assay development\u003c\/li\u003e\n\u003cli\u003eMammalian tissue dissociation workflows\u003c\/li\u003e\n\u003cli\u003eCancer research\u003c\/li\u003e\n\u003cli\u003eImmunology\u003c\/li\u003e\n\u003cli\u003eCell culture research\u003c\/li\u003e\n\u003cli\u003eDrug discovery assays\u003c\/li\u003e\n\u003cli\u003eTranslational biomedical research\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKey Applications\u003c\/h3\u003e\n\u003cp\u003eThe prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDead cell removal from single-cell suspensions\u003c\/li\u003e\n\u003cli\u003eFlow cytometry analysis\u003c\/li\u003e\n\u003cli\u003eSingle-cell sequencing preparation\u003c\/li\u003e\n\u003cli\u003eCell culture workflows\u003c\/li\u003e\n\u003cli\u003eCell counting and quality control\u003c\/li\u003e\n\u003cli\u003ePost-dissociation sample cleanup\u003c\/li\u003e\n\u003cli\u003eCell-based assays\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWorkflow and Sample-Preparation Value\u003c\/h3\u003e\n\u003cp\u003eThe processed cell suspension is centrifuged and resuspended in RPMI 1640 medium containing 2% FBS, then mixed 1:1 with DCRS. The mixture is layered over DCRS and centrifuged at 4°C, 700 ×g for 20 minutes. The upper liquid is collected without disturbing the pellet, washed with PBS containing 5% FBS, and resuspended for downstream use.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eDead Cell Removal Solution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBrand\u003c\/td\u003e\n\u003ctd\u003eFireGene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalog No.\u003c\/td\u003e\n\u003ctd\u003eFG-BA3338\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit Size\u003c\/td\u003e\n\u003ctd\u003e10 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Type\u003c\/td\u003e\n\u003ctd\u003eSingle-cell suspensions from dissociated mammalian tissues\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Function\u003c\/td\u003e\n\u003ctd\u003eRemoval of dead cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWorkflow\u003c\/td\u003e\n\u003ctd\u003eCentrifugation, DCRS layering, separation, upper-layer collection, washing, resuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDownstream Applications\u003c\/td\u003e\n\u003ctd\u003eFlow cytometry analysis, single-cell sequencing, cell culture, cell-related assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSeparation Conditions\u003c\/td\u003e\n\u003ctd\u003e4°C, 700 ×g, 20 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Instruments\u003c\/td\u003e\n\u003ctd\u003eHorizontal centrifuge, cell counter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Reagents\u003c\/td\u003e\n\u003ctd\u003ePBS, FBS, RPMI 1640 medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Consumables\u003c\/td\u003e\n\u003ctd\u003eLow-adhesion pipette tips, centrifuge tubes, 20 μm cell strainers\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOptional Related Reagent\u003c\/td\u003e\n\u003ctd\u003eRed Blood Cell Lysis Solution, Catalog No. BA3311\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e4°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf Life\u003c\/td\u003e\n\u003ctd\u003eOne year\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eResearch Use\u003c\/td\u003e\n\u003ctd\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eCatalog Number\u003c\/th\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eDCRS (Dead Cell Removal Solution)\u003c\/td\u003e\n\u003ctd\u003eFG-BA3338\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"FireGene","offers":[{"title":"10 rxns","offer_id":47868495790292,"sku":"FG-BA3338-10","price":329.0,"currency_code":"USD","in_stock":true},{"title":"40 rxns","offer_id":47868495823060,"sku":"FG-BA3338-40","price":919.0,"currency_code":"USD","in_stock":true}]},{"product_id":"digestive-tissue-cell-debris-removal-kit","title":"FireGene Digestive Tissue Cell Debris Removal Kit for Single-Cell Cleanup","description":"\u003cp\u003e\u003cstrong\u003eFireGene Digestive Tissue Cell Debris Removal Kit\u003c\/strong\u003e is designed to remove dead cells, impurities, and cell debris from single-cell suspensions generated after dissociation of mammalian digestive system tissues. The workflow helps produce a cleaner background and lower debris content for downstream experiments such as flow cytometry analysis, single-cell sequencing, cell culture, and other cell-related assays. The protocol uses layered separation with DRS ① and DRS ②, followed by viable cell layer collection, washing, and quality control.\u003c\/p\u003e\n\u003ch2\u003eBackground Information\u003c\/h2\u003e\n\u003cp\u003eDigestive system tissues can generate debris, impurities, mucus-associated material, and dead cells during dissociation. A dedicated cleanup workflow helps improve suspension clarity before downstream single-cell, flow cytometry, or culture-based analysis.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Digestive Tissue Cell Debris Removal Kit for Single-Cell Cleanup\u003c\/strong\u003e provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.\u003c\/p\u003e\n\u003ch3\u003eResearch Areas\u003c\/h3\u003e\n\u003cp\u003eThis product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eGastrointestinal biology\u003c\/li\u003e\n\u003cli\u003eDigestive disease research\u003c\/li\u003e\n\u003cli\u003eMucosal immunology\u003c\/li\u003e\n\u003cli\u003eInflammation research\u003c\/li\u003e\n\u003cli\u003eCancer and tumor microenvironment studies\u003c\/li\u003e\n\u003cli\u003eSingle-cell biology\u003c\/li\u003e\n\u003cli\u003eCell culture workflows\u003c\/li\u003e\n\u003cli\u003eTranslational biomedical research\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKey Applications\u003c\/h3\u003e\n\u003cp\u003eThe prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDigestive tissue cell debris removal\u003c\/li\u003e\n\u003cli\u003eSingle-cell suspension cleanup\u003c\/li\u003e\n\u003cli\u003eFlow cytometry analysis\u003c\/li\u003e\n\u003cli\u003eSingle-cell sequencing preparation\u003c\/li\u003e\n\u003cli\u003eCell culture workflows\u003c\/li\u003e\n\u003cli\u003eCell viability and counting\u003c\/li\u003e\n\u003cli\u003ePost-dissociation sample processing\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eDigestive System Tissue Cell Debris Removal Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBrand\u003c\/td\u003e\n\u003ctd\u003eFireGene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalog No.\u003c\/td\u003e\n\u003ctd\u003eFG-BA3329\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit Size\u003c\/td\u003e\n\u003ctd\u003e10 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Type\u003c\/td\u003e\n\u003ctd\u003eSingle-cell suspensions from mammalian digestive system tissues\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Function\u003c\/td\u003e\n\u003ctd\u003eRemoval of dead cells, impurities, and cell debris\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWorkflow\u003c\/td\u003e\n\u003ctd\u003eCentrifugation, DRS ① mixing, DRS ② layering, separation, viable cell layer collection, washing, resuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDownstream Applications\u003c\/td\u003e\n\u003ctd\u003eFlow cytometry analysis, single-cell sequencing, cell culture, cell-related assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSeparation Conditions\u003c\/td\u003e\n\u003ctd\u003e4°C, 1400 ×g, 20 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Instruments\u003c\/td\u003e\n\u003ctd\u003eHorizontal centrifuge, cell counter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Reagents\u003c\/td\u003e\n\u003ctd\u003eRPMI 1640 medium, PBS, FBS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Consumables\u003c\/td\u003e\n\u003ctd\u003eLow-adhesion pipette tips, centrifuge tubes, 20 μm cell strainers\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOptional Related Reagent\u003c\/td\u003e\n\u003ctd\u003eRed Blood Cell Lysis Solution, Catalog No. BA3311\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e4°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf Life\u003c\/td\u003e\n\u003ctd\u003eOne year\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eResearch Use\u003c\/td\u003e\n\u003ctd\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eCatalog Number\u003c\/th\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e(DRS ①) Digestive System Tissue Cell Debris Removal Solution ①\u003c\/td\u003e\n\u003ctd\u003eFG-BA3329-A\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e(DRS ②) Digestive System Tissue Cell Debris Removal Solution ②\u003c\/td\u003e\n\u003ctd\u003eFG-BA3329-B\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e20 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"FireGene","offers":[{"title":"10 rxns","offer_id":47868495855828,"sku":"FG-BA3329-10","price":589.0,"currency_code":"USD","in_stock":true},{"title":"50 rxns","offer_id":47868495888596,"sku":"FG-BA3329-50","price":1769.0,"currency_code":"USD","in_stock":true}]},{"product_id":"brain-tissue-cell-debris-removal-kit","title":"FireGene Brain Tissue Cell Debris Removal Kit for Single-Cell Cleanup","description":"\u003cp\u003e\u003cstrong\u003eFireGene Brain Tissue Cell Debris Removal Kit\u003c\/strong\u003e is designed to remove debris from single-cell suspensions generated after mammalian brain tissue dissociation. The workflow helps reduce debris background before downstream experiments such as flow cytometry analysis and single-cell sequencing. The protocol uses Brain Tissue Cell Debris Removal Solution with layered centrifugation, followed by debris layer removal, washing, optional red blood cell lysis, optional 20 μm filtration when clumping is present, and final quality control.\u003c\/p\u003e\n\u003ch2\u003eBackground Information\u003c\/h2\u003e\n\u003cp\u003eBrain tissue dissociation often produces debris that can affect downstream cell analysis. A dedicated debris removal step helps improve sample background before flow cytometry, single-cell sequencing, and other cell-based workflows.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Brain Tissue Cell Debris Removal Kit for Single-Cell Cleanup\u003c\/strong\u003e provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.\u003c\/p\u003e\n\u003ch3\u003eResearch Areas\u003c\/h3\u003e\n\u003cp\u003eThis product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eNeuroscience research\u003c\/li\u003e\n\u003cli\u003eBrain tissue dissociation workflows\u003c\/li\u003e\n\u003cli\u003eNeuroinflammation studies\u003c\/li\u003e\n\u003cli\u003eNeurodegeneration research\u003c\/li\u003e\n\u003cli\u003eGlial and neuronal cell analysis\u003c\/li\u003e\n\u003cli\u003eSingle-cell biology\u003c\/li\u003e\n\u003cli\u003eFlow cytometry assay development\u003c\/li\u003e\n\u003cli\u003eTranslational neurobiology\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKey Applications\u003c\/h3\u003e\n\u003cp\u003eThe prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eBrain tissue cell debris removal\u003c\/li\u003e\n\u003cli\u003eSingle-cell suspension cleanup\u003c\/li\u003e\n\u003cli\u003eFlow cytometry analysis\u003c\/li\u003e\n\u003cli\u003eSingle-cell sequencing preparation\u003c\/li\u003e\n\u003cli\u003eCell counting and quality control\u003c\/li\u003e\n\u003cli\u003ePost-dissociation sample processing\u003c\/li\u003e\n\u003cli\u003eNeural cell population analysis\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eBrain Tissue Cell Debris Removal Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBrand\u003c\/td\u003e\n\u003ctd\u003eFireGene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalog No.\u003c\/td\u003e\n\u003ctd\u003eFG-BA3330\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit Size\u003c\/td\u003e\n\u003ctd\u003e10 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Type\u003c\/td\u003e\n\u003ctd\u003eSingle-cell suspensions from mammalian brain tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Function\u003c\/td\u003e\n\u003ctd\u003eRemoval of debris from brain tissue cell suspensions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWorkflow\u003c\/td\u003e\n\u003ctd\u003eCentrifugation, DRS treatment, layered separation, debris removal, washing, optional RBC lysis, optional filtration, resuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDownstream Applications\u003c\/td\u003e\n\u003ctd\u003eFlow cytometry analysis, single-cell sequencing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSeparation Conditions\u003c\/td\u003e\n\u003ctd\u003e4°C, 3000 ×g, 20 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Instruments\u003c\/td\u003e\n\u003ctd\u003eHorizontal centrifuge\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Reagents\u003c\/td\u003e\n\u003ctd\u003ePBS, FBS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Consumables\u003c\/td\u003e\n\u003ctd\u003eLow-adhesion pipette tips, centrifuge tubes, 20 μm cell strainers\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOptional Related Reagent\u003c\/td\u003e\n\u003ctd\u003eRed Blood Cell Lysis Solution, Catalog No. BA3311\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e4°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf Life\u003c\/td\u003e\n\u003ctd\u003eOne year\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eResearch Use\u003c\/td\u003e\n\u003ctd\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eCatalog Number\u003c\/th\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eDRS (Brain Tissue Cell Debris Removal Solution)\u003c\/td\u003e\n\u003ctd\u003eFG-BA3330\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"FireGene","offers":[{"title":"10 rxns","offer_id":47868495921364,"sku":"FG-BA3330-10","price":449.0,"currency_code":"USD","in_stock":true},{"title":"50 rxns","offer_id":47868495954132,"sku":"FG-BA3330-50","price":1379.0,"currency_code":"USD","in_stock":true}]}],"url":"https:\/\/firegene.com\/collections\/single-cell-suspension-cleanup-workflow.oembed","provider":"FireGene","version":"1.0","type":"link"}