{"title":"Single-Nucleus RNA Sequencing","description":"\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003cstrong\u003eSingle-Nucleus RNA Sequencing\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003eSome tissues can't be dissociated into single cells without losing most of what you're trying to study. Adipocytes rupture under standard mechanical and enzymatic protocols. Mature cardiomyocytes and skeletal muscle fibers are too large for microfluidic capture. Vascular smooth muscle cells don't survive the dissociation conditions that work for softer tissues. For these tissue types, nuclei isolation isn't a workaround — it's the only viable path to single-cell resolution.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003esnRNA-seq also enables work on frozen archival tissue where intact cell isolation is no longer possible, and on tissues where ex vivo transcriptional stress responses from dissociation would distort the biology you're trying to measure.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003eThis collection covers five nuclei isolation kits optimized for snRNA-seq and snATAC-seq, spanning difficult-to-dissociate tissues and a universal option for general use.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003ca href=\"https:\/\/firegene.com\/products\/multi-tissue-nuclei-isolation-kit-fg-ba3302\"\u003e\u003cstrong\u003eMulti Tissue Nuclei Isolation Kit\u003c\/strong\u003e\u003c\/a\u003e — The entry-level kit for standard frozen tissue inputs across a broad range of tissue types. Starting point for labs new to snRNA-seq or running multi-tissue comparison studies.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003ca href=\"https:\/\/firegene.com\/products\/adipose-tissue-nuclei-isolation-kit\"\u003e\u003cstrong\u003eAdipose Tissue Nuclei Isolation Kit\u003c\/strong\u003e\u003c\/a\u003e — Adipocytes contain large lipid droplets that cause them to float and burst under standard lysis conditions. This kit uses a lipid-tolerant lysis buffer and density-based separation to recover intact nuclei from fat-rich tissue.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003ca href=\"https:\/\/firegene.com\/products\/muscle-nuclei-isolation-kit-single-nucleus-sequencing\"\u003e\u003cstrong\u003eMuscle Nuclei Isolation Kit\u003c\/strong\u003e\u003c\/a\u003e — Adapted for the large, multinucleated cells of skeletal and cardiac muscle that are mechanically resistant and incompatible with standard dissociation chemistries.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003ca href=\"https:\/\/firegene.com\/products\/vascular-tissue-nuclei-isolation-kit\"\u003e\u003cstrong\u003eBlood Vessel Nuclei Isolation Kit\u003c\/strong\u003e\u003c\/a\u003e — Designed for the mixed cell composition and ECM-dense structure of vascular tissue, covering endothelial cells, smooth muscle cells, and pericytes.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003ca href=\"https:\/\/firegene.com\/products\/plant-nuclei-isolation-kit-snrna-seq\"\u003e\u003cstrong\u003eUniversal Plant Nuclei Isolation Kit\u003c\/strong\u003e\u003c\/a\u003e — Nuclei isolation from plant tissue for snRNA-seq, bypassing the cell wall challenge that makes whole-cell plant single-cell workflows impractical for most species.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003eAll five kits are validated for 10x Genomics Chromium, BD Rhapsody, and Drop-seq workflows.\u003c\/p\u003e","products":[{"product_id":"multi-tissue-nuclei-isolation-kit-fg-ba3302","title":"FireGene Multi Tissue Nuclei Isolation Kit for Single-Cell Sequencing","description":"\u003ch3 class=\"\" data-end=\"192\" data-start=\"180\"\u003e\u003cbr\u003e\u003c\/h3\u003e\n\u003ch3 class=\"\" data-end=\"192\" data-start=\"180\"\u003eOverview\u003c\/h3\u003e\n\u003cp class=\"\" data-end=\"396\" data-start=\"193\"\u003eFireGene’s \u003cstrong data-end=\"241\" data-start=\"204\"\u003eMulti Tissue Nuclei Isolation Kit\u003c\/strong\u003e is designed for isolating high-quality nuclei from various animal tissues, enabling efficient single-cell sequencing and chromatin accessibility analysis.\u003c\/p\u003e\n\u003ch3 class=\"\" data-end=\"424\" data-start=\"398\"\u003eBackground Information\u003c\/h3\u003e\n\u003cul data-end=\"873\" data-start=\"425\"\u003e\n\u003cli class=\"\" data-end=\"487\" data-start=\"425\"\u003e\n\u003cp class=\"\" data-end=\"487\" data-start=\"427\"\u003eIdeal for gene expression and regulatory mechanism research.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli class=\"\" data-end=\"550\" data-start=\"488\"\u003e\n\u003cp class=\"\" data-end=\"550\" data-start=\"490\"\u003ePreserves cellular integrity by avoiding harsh dissociation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli class=\"\" data-end=\"615\" data-start=\"551\"\u003e\n\u003cp class=\"\" data-end=\"615\" data-start=\"553\"\u003ePrevents loss of rare or fragile cell types during processing.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli class=\"\" data-end=\"704\" data-start=\"616\"\u003e\n\u003cp class=\"\" data-end=\"704\" data-start=\"618\"\u003eFacilitates freezing and reanalysis of biobanked samples without compromising quality.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli class=\"\" data-end=\"776\" data-start=\"705\"\u003e\n\u003cp class=\"\" data-end=\"776\" data-start=\"707\"\u003eEnhances reproducibility and efficiency across multiple tissue types.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli class=\"\" data-end=\"873\" data-start=\"777\"\u003e\n\u003cp class=\"\" data-end=\"873\" data-start=\"779\"\u003eCompatible with Single Cell ATAC, Multiome ATAC + Gene Expression, and Gene Expression assays.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"\" data-end=\"898\" data-start=\"875\"\u003eDetection Principle\u003c\/h3\u003e\n\u003cul data-end=\"1130\" data-start=\"899\"\u003e\n\u003cli class=\"\" data-end=\"984\" data-start=\"899\"\u003e\n\u003cp class=\"\" data-end=\"984\" data-start=\"901\"\u003eBegins with lysis of cells to break the membrane while retaining nuclear structure.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli class=\"\" data-end=\"1058\" data-start=\"985\"\u003e\n\u003cp class=\"\" data-end=\"1058\" data-start=\"987\"\u003eUses \u003cstrong data-end=\"1027\" data-start=\"992\"\u003edensity gradient centrifugation\u003c\/strong\u003e to separate nuclei by density.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli class=\"\" data-end=\"1130\" data-start=\"1059\"\u003e\n\u003cp class=\"\" data-end=\"1130\" data-start=\"1061\"\u003eYields \u003cstrong data-end=\"1095\" data-start=\"1068\"\u003epurified, intact nuclei\u003c\/strong\u003e ready for downstream applications.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"\" data-end=\"898\" data-start=\"875\"\u003eSpecifications\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eSingle-cell nuclei sequencing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eCompatible Sample Types\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eAnimal tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eSupported Instruments\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eWater bath, horizontal centrifuge, cell counter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e4 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eShelf-life\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 class=\"\" data-end=\"424\" data-start=\"398\"\u003eKit Components\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"color: rgb(0, 0, 0); background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e10 reactions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40.0716%; font-weight: bold; padding: 8px; border: 1px solid rgb(221, 221, 221);\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 59.9284%; font-weight: bold; padding: 8px; border: 1px solid rgb(221, 221, 221);\"\u003e10 Reactions\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 40.0716%;\"\u003eLysis buffer\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 59.9284%;\"\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 40.0716%;\"\u003eFront suspension\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 59.9284%;\"\u003e15 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 40.0716%;\"\u003eRear suspension\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 59.9284%;\"\u003e50 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 40.0716%;\"\u003ePB1\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 59.9284%;\"\u003e3 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 40.0716%;\"\u003ePB2\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 59.9284%;\"\u003e6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 40.0716%;\"\u003ePB3\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 59.9284%;\"\u003e6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"color: rgb(0, 0, 0); background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e50 reactions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px; height: 137.157px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 40.0716%; font-weight: bold; padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 59.9284%; font-weight: bold; padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px;\"\u003e50 Reactions\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 40.0716%; height: 19.5938px;\"\u003eLysis buffer\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 59.9284%; height: 19.5938px;\"\u003e5*10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 40.0716%; height: 19.5938px;\"\u003eFront suspension\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 59.9284%; height: 19.5938px;\"\u003e5*15 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 40.0716%; height: 19.5938px;\"\u003eRear suspension\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 59.9284%; height: 19.5938px;\"\u003e5*50 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 40.0716%; height: 19.5938px;\"\u003ePB1\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 59.9284%; height: 19.5938px;\"\u003e5*3 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 40.0716%; height: 19.5938px;\"\u003ePB2\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 59.9284%; height: 19.5938px;\"\u003e5*6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 40.0716%; height: 19.5938px;\"\u003ePB3\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 59.9284%; height: 19.5938px;\"\u003e5*6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 id=\"detection-principle\"\u003eProduct FAQ\u003c\/h3\u003e\n\u003cdiv class=\"type-page\"\u003e\n\u003cdiv class=\"product-content\"\u003e\n\u003cdiv class=\"con-wrap\"\u003e\n\u003cdiv class=\"jianjie tab selected\"\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e 1. Q: Is this kit only suitable for fresh animal tissues? Can it be used for human-derived tissues or frozen tissues?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: It is not only suitable for fresh animal tissues, but also can be used for cell suspensions of humans or other mammals, as well as frozen tissues. Whether for fresh samples or frozen samples, they must be processed in accordance with the steps in the instruction manual before subsequent operations such as grinding and lysis are carried out to ensure the effect of nucleus extraction.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e2. Q: When adding BSA to the lysis buffer, pre-suspension and post-suspension in the kit, the required final concentrations are different. How to specifically calculate the amount of BSA to add?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: The amount to add should be calculated based on the actual volume of reagents used. For example, if 10 mL of lysis buffer is used and the required final concentration of BSA is 1%, and the concentration of the original BSA solution is 10%, then the volume of BSA to add = (10 mL × 1%) ÷ 10% = 1 mL; if 15 mL of pre-suspension is used, the required final concentration of BSA is 1.5%, and the concentration of the original BSA solution is 10%, then the volume to add = (15 mL × 1.5%) ÷ 10% = 2.25 mL. It should be noted that the reagents should be prepared immediately before use according to the volume required for the sample to ensure accurate concentration (use the original BSA solution directly; otherwise, the density will be affected).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e3. Q: For nucleus RNA-related experiments, there are clear standards for adding RNase inhibitors. Is it necessary to add them for non-RNA-related experiments? What are the impacts if it is not added as required?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: It is not necessary to add RNase inhibitors for non-RNA-related experiments. If RNase inhibitors are not added or the added amount is insufficient in RNA-related experiments, the RNA in the sample will be degraded by RNase, making it impossible to obtain valid data in subsequent RNA-related experiments such as single-nucleus transcriptome sequencing, which directly affects the accuracy of experimental results. For non-RNA-related experiments, since RNA detection is not involved, not adding RNase inhibitors will not affect nucleus extraction and subsequent experiments (such as single-cell ATAC sequencing).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e4.    Q: The operation steps mention that after grinding, the sample should be left to stand for 2-3 minutes and no more than 3 minutes. What problems will occur if the standing time is too short or too long?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: If the standing time is too short, the separation between cell debris and nuclei in the tissue homogenate will be insufficient. During subsequent filtration, the nuclei are likely to be wrapped by impurities, affecting purity. If the standing time is too long, some nuclei may experience decreased nuclear membrane stability and even rupture due to being in the lysis buffer environment for a long time, reducing the nucleus yield. Therefore, the standing time must be strictly controlled within 2-3 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e5. Q: The centrifugation step has strict requirements on the type and parameters of the centrifuge. What consequences will there be if a vertical centrifuge is used instead of a horizontal centrifuge, or if the acceleration and deceleration are not set as required?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: A horizontal centrifuge must be used. A vertical centrifuge cannot make the sample stratify evenly during centrifugation, leading to incomplete separation between nuclei and impurities and affecting extraction purity. If the acceleration and deceleration are not set to the medium level as required, excessively fast acceleration at the initial stage of centrifugation or excessively fast deceleration at the later stage will generate a large centrifugal force impact, which may damage the nuclear structure and cause nuclear rupture. At the same time, it will also affect the solution stratification effect, making it difficult to accurately collect the nuclear layer.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e6. Q: Step 7 and Step 11 mention the use of 5 mL and 15 mL centrifuge tubes respectively. Can centrifuge tubes of other specifications be used as substitutes? What problems may occur after substitution?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Substitution is not recommended. In Step 7, 600 μL of mixed solution is transferred to a 5 mL centrifuge tube. This specification of centrifuge tube can provide sufficient space for the subsequent addition of PB2, PB3 and solution stratification, and is easy to operate. If a smaller specification (such as 2 mL) is used, the solution is prone to overflow and stratification will be unclear. In Step 11, a 15 mL centrifuge tube is used to collect the filtrate, which can hold a large amount of filtrate. If a 5 mL centrifuge tube is used, multiple transfers may be required, increasing the risk of contamination and being unfavorable for subsequent centrifugation operations.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e7. Q: The self-prepared materials include 40 μm and 10 μm cell sieves. What are the different application scenarios of these two sieves? Can only one of them be used?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The 40 μm cell sieve is used for the initial filtration of the ground sample, mainly to remove large tissue debris and ensure the preliminary purification of nuclei in the filtrate. The 10 μm cell sieve is used during quality control: if there are debris larger than nuclei observed under bright field and the amount of nuclei is sufficient, it is used to further remove impurities and improve purity. It is not allowed to use only one of them. If only the 40 μm sieve is used, small impurities may remain and affect subsequent experiments; if only the 10 μm sieve is used, large tissue debris is likely to block the sieve during initial filtration, resulting in difficult filtration and reduced experimental efficiency.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e8. Q: The kit should be stored at 4°C in the dark. Will short-term storage (e.g., 1-2 hours) at room temperature affect the reagent performance and experimental results?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Short-term storage at room temperature for 1-2 hours usually will not seriously affect the reagent performance, but direct sunlight should be avoided. If the room temperature is relatively high (e.g., exceeding 25°C), long-term storage may lead to decreased stability of some reagent components. For example, the activity of surfactants in the lysis buffer may decrease, affecting the effect of cell membrane disruption; the components in the pre-suspension and post-suspension may undergo slight changes, affecting nucleus preservation. Therefore, after taking out the reagents, they should be used as soon as possible, and the unused ones should be put back into the 4°C refrigerator in time.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e9.    Q: In Step 14, the volume of post-suspension is selected to resuspend the precipitate according to the required nucleus concentration. How to determine whether the required concentration is appropriate? How to adjust if the concentration is too high or too low?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The appropriate concentration should be determined according to the requirements of subsequent experiments. For example, single-nucleus transcriptome sequencing usually requires a nucleus concentration of 1000-5000 cells\/μL. The concentration can be determined by counting with a cell counter. If the concentration is too high, an appropriate amount of post-suspension can be added for dilution. After each addition, the solution should be fully mixed and recounted until the target concentration is reached. If the concentration is too low, the suspension can be centrifuged at 300×g at 4°C for 5 minutes, part of the supernatant can be discarded, and then a small amount of post-suspension can be used to resuspend the precipitate to increase the concentration.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e10. Q: If reagents or samples are accidentally contaminated during the experiment, what remedial measures are available? If remediation is not possible, is it necessary to repurchase the kit?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: If there is obvious contamination inside the samples or reagents (such as turbidity, peculiar smell), there are no effective remedial measures, and the contaminated samples and reagents must be discarded. If the contaminated reagent is a key component of the kit and cannot be replaced, it is necessary to repurchase the kit to avoid the impact of contamination on experimental results and the failure of the experiment.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cfooter\u003e\n\u003cdiv class=\"footer-head pt50 pb50 sm-dn\"\u003e\n\u003cdiv class=\"mauto dflr pt25 pb10\"\u003e\n\u003cdl\u003e\u003c\/dl\u003e\n\u003cdl\u003e\n\u003cdd\u003e\u003c\/dd\u003e\n\u003c\/dl\u003e\n\u003cdiv class=\"txt pl40 pr20 li25\"\u003e\n\u003cdiv class=\"footer-logo\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/footer\u003e","brand":"FireGene","offers":[{"title":"2 reactions\/kit","offer_id":47833285722324,"sku":"FG-BA3302-2rxns","price":79.0,"currency_code":"USD","in_stock":false},{"title":"10 reactions\/kit","offer_id":46299523514580,"sku":"FG-BA3302-10rxns","price":349.0,"currency_code":"USD","in_stock":true},{"title":"50 reactions\/kit","offer_id":47704376377556,"sku":"FG-BA3302-50rxns","price":1149.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/files\/BA3302_807dd298-101e-4750-b134-752a83e7c22e.png?v=1774229854"},{"product_id":"muscle-nuclei-isolation-kit-single-nucleus-sequencing","title":"FireGene Muscle Nuclei Isolation Kit for Single-Nucleus Sequencing","description":"\u003cp\u003e\u003cstrong\u003eFireGene Muscle Nuclei Isolation Kit\u003c\/strong\u003e is designed for the isolation and extraction of nuclei from human or mammalian muscle tissue, including fresh and frozen samples. The kit uses surfactants to disrupt cell membranes and release nuclei while maintaining nuclear membrane integrity. Through tissue grinding, filtration, centrifugation, and density-layer separation, the workflow produces clean single-nucleus suspensions suitable for single-nucleus transcriptome sequencing, single-nucleus ATAC sequencing, and related nuclear analysis experiments.\u003c\/p\u003e\n\u003ch2\u003eBackground Information\u003c\/h2\u003e\n\u003cp data-start=\"1264\" data-end=\"1549\"\u003eMuscle tissue is dense and fibrous, which can make whole-cell dissociation challenging. Nuclei isolation offers a practical sample-preparation approach for muscle research, especially when working with frozen tissue or samples that are difficult to dissociate into intact viable cells.\u003c\/p\u003e\n\u003cp data-start=\"1551\" data-end=\"1736\"\u003eThis kit supports nuclei extraction from both \u003cstrong data-start=\"1597\" data-end=\"1631\"\u003efresh and frozen muscle tissue\u003c\/strong\u003e, helping researchers prepare single-nucleus suspensions for sequencing and molecular analysis workflows.\u003c\/p\u003e\n\u003ch3 data-section-id=\"15x6sgr\" data-start=\"1738\" data-end=\"1756\"\u003eResearch Areas\u003c\/h3\u003e\n\u003cp data-start=\"1758\" data-end=\"1888\"\u003eThe kit is suitable for research fields involving muscle structure, cellular diversity, gene regulation, and tissue heterogeneity.\u003c\/p\u003e\n\u003cul data-start=\"1890\" data-end=\"2179\"\u003e\n\u003cli data-section-id=\"14n1dst\" data-start=\"1890\" data-end=\"1915\"\u003eSkeletal muscle biology\u003c\/li\u003e\n\u003cli data-section-id=\"inffk0\" data-start=\"1916\" data-end=\"1953\"\u003eMuscle development and regeneration\u003c\/li\u003e\n\u003cli data-section-id=\"bj9e1o\" data-start=\"1954\" data-end=\"1986\"\u003eNeuromuscular disease research\u003c\/li\u003e\n\u003cli data-section-id=\"56mux0\" data-start=\"1987\" data-end=\"2017\"\u003eAging-related muscle studies\u003c\/li\u003e\n\u003cli data-section-id=\"fvk3gu\" data-start=\"2018\" data-end=\"2044\"\u003eMetabolic disease models\u003c\/li\u003e\n\u003cli data-section-id=\"9wj5ta\" data-start=\"2045\" data-end=\"2075\"\u003eExercise physiology research\u003c\/li\u003e\n\u003cli data-section-id=\"15qp2sv\" data-start=\"2076\" data-end=\"2099\"\u003eRegenerative medicine\u003c\/li\u003e\n\u003cli data-section-id=\"10aof08\" data-start=\"2100\" data-end=\"2135\"\u003eTranslational biomedical research\u003c\/li\u003e\n\u003cli data-section-id=\"x5rqu8\" data-start=\"2136\" data-end=\"2179\"\u003eSingle-cell and single-nucleus multiomics\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 data-section-id=\"1fuj4ew\" data-start=\"2181\" data-end=\"2201\"\u003eKey Applications\u003c\/h3\u003e\n\u003cp data-start=\"2203\" data-end=\"2309\"\u003eThe prepared nuclei suspensions can be used in downstream experiments that require clean nuclear material.\u003c\/p\u003e\n\u003cul data-start=\"2311\" data-end=\"2620\"\u003e\n\u003cli data-section-id=\"2haltg\" data-start=\"2311\" data-end=\"2356\"\u003eSingle-nucleus RNA sequencing, or snRNA-seq\u003c\/li\u003e\n\u003cli data-section-id=\"9vqous\" data-start=\"2357\" data-end=\"2404\"\u003eSingle-nucleus ATAC sequencing, or snATAC-seq\u003c\/li\u003e\n\u003cli data-section-id=\"1vdl0d5\" data-start=\"2405\" data-end=\"2437\"\u003eNuclear transcriptome analysis\u003c\/li\u003e\n\u003cli data-section-id=\"cxmgau\" data-start=\"2438\" data-end=\"2471\"\u003eChromatin accessibility studies\u003c\/li\u003e\n\u003cli data-section-id=\"oe9er5\" data-start=\"2472\" data-end=\"2504\"\u003eCell-type composition analysis\u003c\/li\u003e\n\u003cli data-section-id=\"14lu26y\" data-start=\"2505\" data-end=\"2567\"\u003eGene expression profiling from fresh or frozen muscle tissue\u003c\/li\u003e\n\u003cli data-section-id=\"kgsayk\" data-start=\"2568\" data-end=\"2620\"\u003eTissue heterogeneity and disease mechanism studies\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003eSpecifications\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"width: 26.7148%;\"\u003eSpecification\u003c\/th\u003e\n\u003cth style=\"width: 72.9242%;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.7148%;\"\u003eProduct Name\u003c\/td\u003e\n\u003ctd style=\"width: 72.9242%;\"\u003eMuscle Nuclei Isolation Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.7148%;\"\u003eCatalog No.\u003c\/td\u003e\n\u003ctd style=\"width: 72.9242%;\"\u003eFG-BA3315\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.7148%;\"\u003eKit Size\u003c\/td\u003e\n\u003ctd style=\"width: 72.9242%;\"\u003e10 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.7148%;\"\u003eSample Type\u003c\/td\u003e\n\u003ctd style=\"width: 72.9242%;\"\u003eHuman or mammalian muscle tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.7148%;\"\u003eCompatible Samples\u003c\/td\u003e\n\u003ctd style=\"width: 72.9242%;\"\u003eFresh and frozen muscle samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.7148%;\"\u003eRecommended Starting Material\u003c\/td\u003e\n\u003ctd style=\"width: 72.9242%;\"\u003eApproximately 200 mg tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.7148%;\"\u003eMain Function\u003c\/td\u003e\n\u003ctd style=\"width: 72.9242%;\"\u003eIsolation and extraction of cell nuclei\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.7148%;\"\u003eWorkflow\u003c\/td\u003e\n\u003ctd style=\"width: 72.9242%;\"\u003eTissue grinding, lysis, filtration, centrifugation, density-layer separation, nuclei resuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.7148%;\"\u003eDownstream Applications\u003c\/td\u003e\n\u003ctd style=\"width: 72.9242%;\"\u003eSingle-nucleus transcriptome sequencing, single-nucleus ATAC sequencing, related nuclear analysis experiments\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.7148%;\"\u003eRequired Instruments\u003c\/td\u003e\n\u003ctd style=\"width: 72.9242%;\"\u003eHorizontal centrifuge, tissue grinder, cell counter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.7148%;\"\u003eRequired Tissue Tools\u003c\/td\u003e\n\u003ctd style=\"width: 72.9242%;\"\u003eSurgical scissors, scalpels, forceps\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.7148%;\"\u003eRequired Consumables\u003c\/td\u003e\n\u003ctd style=\"width: 72.9242%;\"\u003eGrinding tubes with zirconium beads, 5 mL and 15 mL centrifuge tubes, 40 μm cell strainers, optional 20 μm cell strainers\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.7148%;\"\u003eAdditional Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 72.9242%;\"\u003eRNase inhibitor for nuclear RNA-related experiments\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.7148%;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 72.9242%;\"\u003eMain reagents: 4°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.7148%;\"\u003eAdditive Storage\u003c\/td\u003e\n\u003ctd style=\"width: 72.9242%;\"\u003eAdditive Solution ① and Additive Solution ②: -20°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.7148%;\"\u003eShelf Life\u003c\/td\u003e\n\u003ctd style=\"width: 72.9242%;\"\u003eOne year\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.7148%;\"\u003eResearch Use\u003c\/td\u003e\n\u003ctd style=\"width: 72.9242%;\"\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003eKit Components\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"width: 45.6679%;\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"width: 33.574%;\"\u003eCatalog Number\u003c\/th\u003e\n\u003cth align=\"right\" style=\"width: 19.8556%;\"\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 45.6679%;\"\u003eLysis Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 33.574%;\"\u003eFG-BA3315-A\u003c\/td\u003e\n\u003ctd align=\"right\" style=\"width: 19.8556%;\"\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 45.6679%;\"\u003ePre-Suspension Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 33.574%;\"\u003eFG-BA3315-B\u003c\/td\u003e\n\u003ctd align=\"right\" style=\"width: 19.8556%;\"\u003e15 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 45.6679%;\"\u003ePost-Suspension Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 33.574%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3315-C\u003c\/td\u003e\n\u003ctd align=\"right\" style=\"width: 19.8556%;\"\u003e50 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 45.6679%;\"\u003ePB 1\u003c\/td\u003e\n\u003ctd style=\"width: 33.574%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3315-D\u003c\/td\u003e\n\u003ctd align=\"right\" style=\"width: 19.8556%;\"\u003e3 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 45.6679%;\"\u003ePB 2\u003c\/td\u003e\n\u003ctd style=\"width: 33.574%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3315-E\u003c\/td\u003e\n\u003ctd align=\"right\" style=\"width: 19.8556%;\"\u003e6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 45.6679%;\"\u003ePB 3\u003c\/td\u003e\n\u003ctd style=\"width: 33.574%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3315-F\u003c\/td\u003e\n\u003ctd align=\"right\" style=\"width: 19.8556%;\"\u003e6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 45.6679%;\"\u003eAdditive Solution ①\u003c\/td\u003e\n\u003ctd style=\"width: 33.574%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3315-G\u003c\/td\u003e\n\u003ctd align=\"right\" style=\"width: 19.8556%;\"\u003e4 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 45.6679%;\"\u003eAdditive Solution ②\u003c\/td\u003e\n\u003ctd style=\"width: 33.574%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3315-H\u003c\/td\u003e\n\u003ctd align=\"right\" style=\"width: 19.8556%;\"\u003e150 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"FireGene","offers":[{"title":"10 rxns","offer_id":47868356886740,"sku":"FG-BA3315-10","price":699.0,"currency_code":"USD","in_stock":false},{"title":"50 rxns","offer_id":47868356919508,"sku":"FG-BA3315-50","price":2429.0,"currency_code":"USD","in_stock":true}]},{"product_id":"plant-nuclei-isolation-kit-snrna-seq","title":"FireGene Universal Plant Nuclei Isolation Kit for Single-Nucleus Sequencing","description":"\u003cp\u003e\u003cstrong\u003eFireGene Universal Plant Nuclei Isolation Kit\u003c\/strong\u003e is designed for the isolation and extraction of cell nuclei from cryopreserved tissue samples of higher plants. The kit uses surfactants to release nuclei, remove chloroplasts, and maintain nuclear membrane stability. After filtration, centrifugation, density-layer separation, and chloroplast removal, the workflow generates clean single-nucleus suspensions suitable for single-nucleus transcriptome sequencing, single-cell ATAC sequencing, and related plant nuclear analysis experiments.\u003c\/p\u003e\n\u003ch2\u003eBackground Information\u003c\/h2\u003e\n\u003cp data-start=\"1267\" data-end=\"1583\"\u003ePlant tissues contain rigid cell walls, abundant chloroplasts, and diverse secondary metabolites, which can complicate nuclei preparation. For cryopreserved plant samples, nuclei isolation provides a useful approach for studying gene expression and chromatin accessibility when intact cell recovery is not practical.\u003c\/p\u003e\n\u003cp data-start=\"1585\" data-end=\"1801\"\u003e\u003cstrong data-start=\"1585\" data-end=\"1634\"\u003eFireGene Universal Plant Nuclei Isolation Kit\u003c\/strong\u003e is designed for frozen higher plant tissue samples and supports clean nuclei preparation through lysis, filtration, density-layer separation, and chloroplast removal.\u003c\/p\u003e\n\u003ch3 data-section-id=\"15x6sgr\" data-start=\"1803\" data-end=\"1821\"\u003eResearch Areas\u003c\/h3\u003e\n\u003cp data-start=\"1823\" data-end=\"1952\"\u003eThis kit is suitable for plant research fields focused on nuclear gene regulation, tissue heterogeneity, and molecular profiling.\u003c\/p\u003e\n\u003cul data-start=\"1954\" data-end=\"2245\"\u003e\n\u003cli data-section-id=\"1x9yxmc\" data-start=\"1954\" data-end=\"1979\"\u003ePlant molecular biology\u003c\/li\u003e\n\u003cli data-section-id=\"623sjb\" data-start=\"1980\" data-end=\"2007\"\u003ePlant functional genomics\u003c\/li\u003e\n\u003cli data-section-id=\"90febs\" data-start=\"2008\" data-end=\"2037\"\u003ePlant developmental biology\u003c\/li\u003e\n\u003cli data-section-id=\"1w5o8at\" data-start=\"2038\" data-end=\"2070\"\u003ePlant stress response research\u003c\/li\u003e\n\u003cli data-section-id=\"1603u2b\" data-start=\"2071\" data-end=\"2113\"\u003eCrop science and plant breeding research\u003c\/li\u003e\n\u003cli data-section-id=\"1yybilz\" data-start=\"2114\" data-end=\"2133\"\u003ePlant epigenetics\u003c\/li\u003e\n\u003cli data-section-id=\"1y48arh\" data-start=\"2134\" data-end=\"2167\"\u003ePlant single-nucleus multiomics\u003c\/li\u003e\n\u003cli data-section-id=\"1qvit7r\" data-start=\"2168\" data-end=\"2204\"\u003ePlant tissue heterogeneity studies\u003c\/li\u003e\n\u003cli data-section-id=\"41l8oe\" data-start=\"2205\" data-end=\"2245\"\u003eGene regulation and chromatin research\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 data-section-id=\"1fuj4ew\" data-start=\"2247\" data-end=\"2267\"\u003eKey Applications\u003c\/h3\u003e\n\u003cp data-start=\"2269\" data-end=\"2378\"\u003eThe prepared single-nucleus suspensions can be used in downstream workflows requiring clean nuclear material.\u003c\/p\u003e\n\u003cul data-start=\"2380\" data-end=\"2739\"\u003e\n\u003cli data-section-id=\"2haltg\" data-start=\"2380\" data-end=\"2425\"\u003eSingle-nucleus RNA sequencing, or snRNA-seq\u003c\/li\u003e\n\u003cli data-section-id=\"1bd39xk\" data-start=\"2426\" data-end=\"2470\"\u003eSingle-cell ATAC sequencing, or scATAC-seq\u003c\/li\u003e\n\u003cli data-section-id=\"1vdl0d5\" data-start=\"2471\" data-end=\"2503\"\u003eNuclear transcriptome analysis\u003c\/li\u003e\n\u003cli data-section-id=\"5n45lt\" data-start=\"2504\" data-end=\"2539\"\u003eChromatin accessibility profiling\u003c\/li\u003e\n\u003cli data-section-id=\"10w5ai\" data-start=\"2540\" data-end=\"2571\"\u003eCell-type composition studies\u003c\/li\u003e\n\u003cli data-section-id=\"1tyi7am\" data-start=\"2572\" data-end=\"2630\"\u003eGene expression analysis from cryopreserved plant tissue\u003c\/li\u003e\n\u003cli data-section-id=\"jgrmws\" data-start=\"2631\" data-end=\"2668\"\u003ePlant tissue heterogeneity analysis\u003c\/li\u003e\n\u003cli data-section-id=\"1j4qofn\" data-start=\"2669\" data-end=\"2739\"\u003eMolecular mechanism studies in plant development and stress response\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eUniversal Plant Nuclei Isolation Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalog No.\u003c\/td\u003e\n\u003ctd\u003eFG-BA3319\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit Size\u003c\/td\u003e\n\u003ctd\u003e10 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Type\u003c\/td\u003e\n\u003ctd\u003eHigher plant tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible Samples\u003c\/td\u003e\n\u003ctd\u003eCryopreserved plant tissue samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended Starting Material\u003c\/td\u003e\n\u003ctd\u003eApproximately 200–300 mg frozen tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Function\u003c\/td\u003e\n\u003ctd\u003eIsolation and extraction of plant cell nuclei\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWorkflow\u003c\/td\u003e\n\u003ctd\u003eLiquid nitrogen grinding, lysis, filtration, centrifugation, density-layer separation, chloroplast removal, nuclei resuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDownstream Applications\u003c\/td\u003e\n\u003ctd\u003eSingle-nucleus transcriptome sequencing, single-cell ATAC sequencing, related nuclear analysis experiments\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Instruments\u003c\/td\u003e\n\u003ctd\u003eHorizontal centrifuge, mortar and pestle, cell counter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Tissue Tools\u003c\/td\u003e\n\u003ctd\u003eSurgical scissors, surgical knives, forceps\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Reagent\u003c\/td\u003e\n\u003ctd\u003eRNase inhibitor for nuclear RNA-related experiments\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Consumables\u003c\/td\u003e\n\u003ctd\u003eZirconium beads, low-adsorption pipette tips, 5 mL and 15 mL centrifuge tubes, 70 μm cell strainers, optional 20 μm cell strainers\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProcessing Temperature\u003c\/td\u003e\n\u003ctd\u003eLow-temperature workflow; wet ice or ice blocks recommended\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eMain reagents: 4°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdditive Storage\u003c\/td\u003e\n\u003ctd\u003eAdditive Solution ① and Additive Solution ②: -20°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf Life\u003c\/td\u003e\n\u003ctd\u003eOne year under recommended storage conditions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eResearch Use\u003c\/td\u003e\n\u003ctd\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"width: 52.5271%;\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"width: 26.3538%;\"\u003eCatalog Number\u003c\/th\u003e\n\u003cth align=\"right\" style=\"width: 20.2166%;\"\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 52.5271%;\"\u003eLysis Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 26.3538%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3319-A\u003c\/td\u003e\n\u003ctd align=\"right\" style=\"width: 20.2166%;\"\u003e20 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 52.5271%;\"\u003ePre-Suspension Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 26.3538%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3319-B\u003c\/td\u003e\n\u003ctd align=\"right\" style=\"width: 20.2166%;\"\u003e15 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 52.5271%;\"\u003ePost-Suspension Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 26.3538%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3319-C\u003c\/td\u003e\n\u003ctd align=\"right\" style=\"width: 20.2166%;\"\u003e50 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 52.5271%;\"\u003ePB 1\u003c\/td\u003e\n\u003ctd style=\"width: 26.3538%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3319-D\u003c\/td\u003e\n\u003ctd align=\"right\" style=\"width: 20.2166%;\"\u003e3 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 52.5271%;\"\u003ePB 2\u003c\/td\u003e\n\u003ctd style=\"width: 26.3538%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3319-E\u003c\/td\u003e\n\u003ctd align=\"right\" style=\"width: 20.2166%;\"\u003e6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 52.5271%;\"\u003ePB 3\u003c\/td\u003e\n\u003ctd style=\"width: 26.3538%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3319-F\u003c\/td\u003e\n\u003ctd align=\"right\" style=\"width: 20.2166%;\"\u003e6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 52.5271%;\"\u003eCRS, Chloroplast Removal Solution\u003c\/td\u003e\n\u003ctd style=\"width: 26.3538%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3319-G\u003c\/td\u003e\n\u003ctd align=\"right\" style=\"width: 20.2166%;\"\u003eNot specified\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 52.5271%;\"\u003eAdditive Solution ①\u003c\/td\u003e\n\u003ctd style=\"width: 26.3538%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3319-H\u003c\/td\u003e\n\u003ctd align=\"right\" style=\"width: 20.2166%;\"\u003e4 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 52.5271%;\"\u003eAdditive Solution ②\u003c\/td\u003e\n\u003ctd style=\"width: 26.3538%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3319-I\u003c\/td\u003e\n\u003ctd align=\"right\" style=\"width: 20.2166%;\"\u003e150 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eProduct FAQ\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e1.    Q: Is this kit only suitable for cryopreserved tissues of higher plants? Are there differences in nucleus extraction effects for different types of higher plant tissues (e.g., leaves, roots, stems)? Can it be used for fresh plant tissues or lower plant tissues (e.g., algae)?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The kit is only suitable for cryopreserved tissues of higher plants and is not temporarily applicable to fresh plant tissues or lower plant tissues. Fresh plant tissues contain a large amount of water, which easily causes tissue adhesion during grinding and makes it impossible to form uniform fine powder. The nuclear membrane structure and fiber components of lower plants such as algae are significantly different from those of higher plants, and the kit reagents cannot stabilize the cell nuclei, easily leading to extraction failure. There are slight differences in the extraction effects of cryopreserved tissues from different parts of higher plants: leaf tissues (containing more chloroplasts) require extending the action time of chloroplast lysis buffer to 12 minutes; root and stem tissues have less chloroplast content, and an action time of 8 minutes is sufficient. Both can obtain high-purity cell nuclei with no significant difference in yield.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e2.    Q: The instruction manual requires weighing 150-200mg of frozen tissue. Will insufficient tissue quantity (e.g., 80mg) or excessive tissue quantity (e.g., 250mg) affect the nucleus extraction effect? How to adjust the operation?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Yes, it will affect the effect. ① Insufficient tissue quantity (\u0026lt;120mg): The total number of cell nuclei is small, and the nuclear layer is not obvious during subsequent centrifugation and stratification, which easily leads to collection omission and a yield reduction of more than 50%. It is recommended to combine multiple 80mg tissue samples (to a total weight of 150mg) and follow the conventional steps to ensure the nuclear layer is clearly distinguishable. ② Excessive tissue quantity (\u0026gt;220mg): The space in the 5mL centrifuge tube is limited, and the lysis buffer cannot fully wrap the tissue fine powder, which easily leads to incomplete release of local cell nuclei and excessive local enzymolysis. The tissue needs to be processed in two tubes, with each tube containing 150-180mg of tissue and corresponding to 2mL of lysis buffer, to avoid the impact of tissue crowding on extraction efficiency.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e3.    Q: Step 2 requires grinding the frozen tissue into fine powder in a mortar pre-cooled with dry ice for 30 minutes. What impact will insufficient pre-cooling time of the mortar (e.g., 20 minutes) or thawing of the tissue during grinding have on the experiment?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Insufficient pre-cooling of the mortar (\u0026lt;30 minutes) will cause the temperature of the mortar to rise during grinding, and the tissue is prone to thawing and adhesion, making it impossible to form uniform fine powder. Large tissue blocks are visible to the naked eye, and the release of cell nuclei during subsequent lysis is reduced by more than 40%. If the tissue thaws during grinding, plant cells will release a large number of enzymes due to the sudden temperature rise, damaging the nuclear membrane. After centrifugation, the nuclear layer is turbid, and the purity decreases by 60%. It is necessary to ensure that the mortar is pre-cooled for more than 30 minutes, and the tissue is kept in a frozen state throughout the grinding process. Dry ice can be added intermittently to maintain low temperature and prevent tissue thawing.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e4.    Q: Steps 4 and 17 use 70μm and 20μm cell sieves respectively. If the laboratory lacks one type of sieve, can it be replaced with a sieve of another pore size? For example, using 50μm instead of 70μm, or 10μm instead of 20μm?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Random replacement is not allowed. ① The 70μm sieve is used to filter tissue debris after grinding. If replaced with a 50μm sieve, some cell nuclei will be retained (the diameter of higher plant cell nuclei is about 5-10μm; although they can pass through, the sieve is easily blocked by fiber debris, leading to filtration difficulties and even squeezing the cell nuclei to reduce activity). ② The 20μm sieve is used to remove impurities larger than cell nuclei. If replaced with a 10μm sieve, some cell nuclei will be retained, and the yield will decrease by 30%. If a 70μm sieve is lacking, extend the grinding time to 10 minutes to ensure the tissue fine powder is finer, then filter with an 80μm sieve (with an effect close to 70μm). If a 20μm sieve is lacking, let the resuspended nuclear suspension stand for 5 minutes after centrifugation, and aspirate the upper clearer liquid (avoiding bottom precipitated impurities) to minimize debris residue as much as possible.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e5.    Q: The centrifugation parameters for Steps 6, 10, and 13 are \"4℃, 500×g, 5 minutes\", \"4℃, 3000×g, 20 minutes\", and \"4℃, 700×g, 5 minutes\" respectively. What impact will deviations in centrifugation speed or time have? Can a vertical centrifuge replace a horizontal centrifuge?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Deviations in centrifugation parameters have a significant impact: ① Step 6: If the speed is too low (\u0026lt;400×g), the cell nuclei cannot be fully precipitated and are discarded with the supernatant, resulting in a 40% yield reduction; if the speed is too high (\u0026gt;600×g), the centrifugal force is too strong, squeezing the cell nuclei and causing nuclear membrane rupture, with a 25% decrease in activity. ② Step 10: If the time is insufficient (\u0026lt;15 minutes), the solution stratification is unclear, and the nuclear layer is mixed with the impurity layer, making accurate collection impossible; if the speed is too low (\u0026lt;2800×g), the nuclear layer cannot settle at the interface of PB2 and PB3, resulting in a sharp 50% yield reduction. ③ Step 13: If the speed is too high (\u0026gt;800×g), the nuclear precipitate is compacted, making subsequent resuspension difficult. A vertical centrifuge cannot replace a horizontal centrifuge. The direction of centrifugal force of a vertical centrifuge is perpendicular to the centrifuge tube, which will cause disordered stratification of the solution and dispersion of the nuclear layer, making it impossible to form a clear three-layer structure. A horizontal centrifuge must be used.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e6.    Q: Step 2 mentions that \"lysis buffer, pre-suspension, and post-suspension need to be added with BSA at a final concentration of 1%\". What impact will deviations in BSA concentration (e.g., 0.5% or 2%) or forgetting to add BSA have on the cell nuclei?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The core function of BSA is to protect the nuclear membrane and reduce mechanical damage during operation. ① Low concentration (0.5%): Insufficient protective effect, the nuclear membrane is prone to rupture during centrifugation and resuspension, a large number of nuclear fragments are visible under the microscope, and the purity decreases by 35%. ② High concentration (2%): Excessive BSA increases the solution viscosity, slows down the flow rate during subsequent filtration, and is easily mixed with cell nuclei, affecting downstream sequencing experiments (e.g., increasing non-specific signals). Forgetting to add BSA will cause the nuclear membrane to be directly exposed to the reagents, suffering double damage from mechanical force and chemical factors, with an activity reduction of more than 60%, making it unusable for subsequent experiments and requiring re-extraction.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e7.    Q: For nucleus RNA-related experiments, it is necessary to add RNase inhibitor at a final concentration of 1U\/μL to all reagents. How to specifically calculate the addition amount? What consequences will insufficient or excessive addition have?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Calculation method for the addition amount: Taking \"per 1mL of reagent\" as the unit, if using 40U\/μL RNase inhibitor, 25μL needs to be added (1mL×1U\/μL÷40U\/μL=25μL). The lysis buffer needs to be additionally adjusted to a final concentration of 1.2U\/μL, that is, 30μL of 40U\/μL RNase inhibitor is added per 1mL of lysis buffer. Insufficient addition (\u0026lt;0.8U\/μL): It cannot effectively inhibit RNase activity, and the nuclear RNA will be degraded. The subsequent RNA extraction amount is less than 20% of the normal amount, and fragmentation is severe. Excessive addition (\u0026gt;1.5U\/μL): The preservative in the RNase inhibitor affects the activity of the cell nuclei, causing partial nuclear membrane rupture and a 20% decrease in purity. The calculated amount must be strictly added.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e8.    Q: Step 14 requires adding chloroplast lysis buffer and placing it on ice for 8-12 minutes, with \"the specific time depending on the amount of chloroplasts\". How to judge the amount of chloroplasts? What impact will insufficient or excessive action time have?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Method for judging the amount of chloroplasts: If the ground tissue fine powder is dark green (e.g., leaf tissue), it indicates a large amount of chloroplasts; if it is light yellow or white (e.g., root tissue), it indicates a small amount of chloroplasts. Adjustment of action time: Dark green tissues require 10-12 minutes, light yellow\/white tissues require 8-10 minutes. During this period, observation can be made under a microscope every 2 minutes, and the process can be terminated when the green particles are significantly reduced. Insufficient action time (\u0026lt;8 minutes): Chloroplasts are not fully lysed, the precipitate is still green after centrifugation, and chloroplast fragments will interfere with nuclear detection in subsequent experiments (e.g., flow cytometry signal deviation). Excessive action time (\u0026gt;15 minutes): The chloroplast lysis buffer will damage the nuclear membrane, leading to a 30% decrease in nuclear activity. The time must be dynamically adjusted according to the color.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e9.    Q: When collecting the nuclear layer in Step 11, how to accurately identify the position of the nuclear layer? What impact will mistakenly aspirating the PB2 layer or debris layer have on subsequent experiments?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: After centrifugation, the solution is divided into three layers: the upper layer is the debris layer (turbid, approximately 1000μL), the middle layer is the nuclear layer (translucent, slightly milky white, approximately 400μL, located at the interface of PB2 and PB3), and the lower layer is the PB3 layer (transparent). During collection, a 1mL low-adhesion pipette tip should be used to slowly insert into the middle layer close to the liquid surface, and only the translucent liquid should be aspirated to avoid touching the upper and lower layers. Mistakenly aspirating the debris layer: A large amount of tissue debris is mixed into the nuclear suspension, causing significant impurity interference during subsequent counting and clogging the single-cell sequencing chip. Mistakenly aspirating the PB2 layer: The high-salt components in PB2 will damage the nuclear membrane, causing nuclear rupture, making it unusable for sequencing or staining experiments, and requiring re-centrifugation for collection.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e10.    Q: The kit needs to be stored at 4℃ in the dark with a validity period of 1 year. If the ice pack melts during transportation and the reagent is left at room temperature (25℃) for 1.5 hours, can it still be used? What consequences will long-term storage at room temperature have?\u003c\/strong\u003e\u003c\/span\u003e\u003cbr\u003e\u003cspan\u003eA: It can still be used after being left at room temperature for 1.5 hours, but it must be immediately returned to the 4℃ refrigerator and fully mixed before use. The surfactants and buffer components in the reagent have good stability during short-term room-temperature storage (≤2 hours), with an activity loss of ≤10%, which does not affect the extraction effect. Long-term storage at room temperature (exceeding 24 hours) will lead to: ① Decreased activity of the lysis buffer, inability to effectively release cell nuclei, and a 50% yield reduction; ② Inactivation of the chloroplast lysis buffer, inability to remove chloroplasts, and a sharp decrease in purity; ③ Changes in the pH value of the buffer, inability to stabilize the nuclear membrane, and easy rupture of cell nuclei during centrifugation. Finally, a qualified nuclear suspension cannot be obtained, and it cannot be used.\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"10 rxns","offer_id":47868382937300,"sku":"FG-BA3319-10","price":789.0,"currency_code":"USD","in_stock":true},{"title":"50 rxns","offer_id":47868382970068,"sku":"FG-BA3319-50","price":2759.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/files\/universal-preparation-of-nucleus-suspension-from-plant-tissuespng.png?v=1778574036"},{"product_id":"vascular-tissue-nuclei-isolation-kit","title":"FireGene Blood Vessel Nuclei Isolation Kit for Single-Nucleus Sequencing","description":"\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e\u003cstrong data-end=\"669\" data-start=\"623\"\u003eFireGene Blood Vessel Nuclei Isolation Kit\u003c\/strong\u003e is designed for the isolation and extraction of nuclei from cell suspensions, fresh tissues, and cryopreserved tissues derived from animal vascular tissues. The kit uses surfactants to disrupt cell membranes and release nuclei while maintaining nuclear membrane integrity. After tissue grinding, filtration, centrifugation, and density-layer separation, the workflow produces clean single-nucleus suspensions suitable for single-nucleus transcriptome sequencing, single-nucleus ATAC sequencing, and related nuclear analysis experiments.\u003c\/p\u003e\n\u003ch2\u003eBackground Information\u003c\/h2\u003e\n\u003cp data-end=\"1670\" data-start=\"1345\"\u003eBlood vessels contain multiple specialized cell populations, including endothelial cells, smooth muscle cells, pericytes, fibroblasts, and immune-associated cells. Preparing nuclei from vascular tissue can support molecular profiling when intact cell dissociation is challenging or when cryopreserved tissue samples are used.\u003c\/p\u003e\n\u003cp data-end=\"1861\" data-start=\"1672\"\u003e\u003cstrong data-end=\"1718\" data-start=\"1672\"\u003eFireGene Blood Vessel Nuclei Isolation Kit\u003c\/strong\u003e provides a practical workflow for preparing clean single-nucleus suspensions from animal vascular samples for sequencing and nuclear analysis.\u003c\/p\u003e\n\u003ch3 data-end=\"1881\" data-start=\"1863\" data-section-id=\"15x6sgr\"\u003eResearch Areas\u003c\/h3\u003e\n\u003cp data-end=\"2008\" data-start=\"1883\"\u003eThis kit is suitable for research fields focused on vascular structure, gene regulation, and tissue-level cellular diversity.\u003c\/p\u003e\n\u003cul data-end=\"2317\" data-start=\"2010\"\u003e\n\u003cli data-end=\"2028\" data-start=\"2010\" data-section-id=\"yufykc\"\u003eVascular biology\u003c\/li\u003e\n\u003cli data-end=\"2062\" data-start=\"2029\" data-section-id=\"1gha1eq\"\u003eCardiovascular disease research\u003c\/li\u003e\n\u003cli data-end=\"2090\" data-start=\"2063\" data-section-id=\"9jlppi\"\u003eEndothelial cell research\u003c\/li\u003e\n\u003cli data-end=\"2119\" data-start=\"2091\" data-section-id=\"1pzo0ic\"\u003eSmooth muscle cell biology\u003c\/li\u003e\n\u003cli data-end=\"2171\" data-start=\"2120\" data-section-id=\"1vdlgwu\"\u003eAtherosclerosis and vascular inflammation studies\u003c\/li\u003e\n\u003cli data-end=\"2195\" data-start=\"2172\" data-section-id=\"lxf1i7\"\u003eAngiogenesis research\u003c\/li\u003e\n\u003cli data-end=\"2237\" data-start=\"2196\" data-section-id=\"uwelti\"\u003eTissue remodeling and fibrosis research\u003c\/li\u003e\n\u003cli data-end=\"2281\" data-start=\"2238\" data-section-id=\"x5rqu8\"\u003eSingle-cell and single-nucleus multiomics\u003c\/li\u003e\n\u003cli data-end=\"2317\" data-start=\"2282\" data-section-id=\"10aof08\"\u003eTranslational biomedical research\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 data-end=\"2339\" data-start=\"2319\" data-section-id=\"1fuj4ew\"\u003eKey Applications\u003c\/h3\u003e\n\u003cp data-end=\"2442\" data-start=\"2341\"\u003eThe prepared nuclei suspensions can be used in downstream workflows requiring clean nuclear material.\u003c\/p\u003e\n\u003cul data-end=\"2825\" data-start=\"2444\"\u003e\n\u003cli data-end=\"2489\" data-start=\"2444\" data-section-id=\"2haltg\"\u003eSingle-nucleus RNA sequencing, or snRNA-seq\u003c\/li\u003e\n\u003cli data-end=\"2537\" data-start=\"2490\" data-section-id=\"9vqous\"\u003eSingle-nucleus ATAC sequencing, or snATAC-seq\u003c\/li\u003e\n\u003cli data-end=\"2570\" data-start=\"2538\" data-section-id=\"1vdl0d5\"\u003eNuclear transcriptome analysis\u003c\/li\u003e\n\u003cli data-end=\"2606\" data-start=\"2571\" data-section-id=\"5n45lt\"\u003eChromatin accessibility profiling\u003c\/li\u003e\n\u003cli data-end=\"2658\" data-start=\"2607\" data-section-id=\"10yh06\"\u003eCell-type composition analysis in vascular tissue\u003c\/li\u003e\n\u003cli data-end=\"2731\" data-start=\"2659\" data-section-id=\"1j6s7iv\"\u003eGene expression profiling from fresh or cryopreserved vascular samples\u003c\/li\u003e\n\u003cli data-end=\"2771\" data-start=\"2732\" data-section-id=\"12qwlyl\"\u003eVascular tissue heterogeneity studies\u003c\/li\u003e\n\u003cli data-end=\"2825\" data-start=\"2772\" data-section-id=\"1gnhqrm\"\u003eDisease mechanism research in cardiovascular models\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003eSpecifications\u003c\/h2\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eBlood Vessel Nuclei Isolation Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBrand\u003c\/td\u003e\n\u003ctd\u003eFireGene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit Size\u003c\/td\u003e\n\u003ctd\u003e10 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Source\u003c\/td\u003e\n\u003ctd\u003eAnimal vascular tissues\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible Samples\u003c\/td\u003e\n\u003ctd\u003eCell suspensions, fresh tissues, and cryopreserved tissues derived from animal vascular tissues\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended Starting Material\u003c\/td\u003e\n\u003ctd\u003eApproximately 200 mg tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Function\u003c\/td\u003e\n\u003ctd\u003eIsolation and extraction of nuclei\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWorkflow\u003c\/td\u003e\n\u003ctd\u003eLiquid nitrogen grinding, lysis, filtration, centrifugation, density-layer separation, nuclei washing and resuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDownstream Applications\u003c\/td\u003e\n\u003ctd\u003eSingle-nucleus transcriptome sequencing, single-nucleus ATAC sequencing, related nuclear analysis experiments\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Instruments\u003c\/td\u003e\n\u003ctd\u003eHorizontal centrifuge, tissue grinder, cell counter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Tissue Tools\u003c\/td\u003e\n\u003ctd\u003eSurgical scissors, scalpels, forceps\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Reagents\u003c\/td\u003e\n\u003ctd\u003eRNase inhibitor for nuclear RNA-related experiments\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Consumables\u003c\/td\u003e\n\u003ctd\u003eLiquid nitrogen, grinding tubes with zirconium beads, 5 mL and 15 mL centrifuge tubes, 40 μm cell strainers, optional 20 μm cell strainers\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProcessing Temperature\u003c\/td\u003e\n\u003ctd\u003ePost-lysis steps should be performed on wet ice or an ice block when possible\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eMain reagents: 4°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdditive Storage\u003c\/td\u003e\n\u003ctd\u003eAdditive Solution ① and Additive Solution ②: -20°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf Life\u003c\/td\u003e\n\u003ctd\u003eOne year\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eResearch Use\u003c\/td\u003e\n\u003ctd\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003eKit Components\u003c\/h2\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eCatalog Number\u003c\/th\u003e\n\u003cth align=\"right\"\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eLysis Buffer\u003c\/td\u003e\n\u003ctd\u003eFG-BA3343-A\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePre-Suspension Buffer\u003c\/td\u003e\n\u003ctd\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3343-B\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e15 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePost-Suspension Buffer\u003c\/td\u003e\n\u003ctd\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3343-C\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e50 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePB 1\u003c\/td\u003e\n\u003ctd\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3343-D\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e3 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePB 2\u003c\/td\u003e\n\u003ctd\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3343-E\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePB 3\u003c\/td\u003e\n\u003ctd\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3343-F\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdditive Solution ①\u003c\/td\u003e\n\u003ctd\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3343-G\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e4 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdditive Solution ②\u003c\/td\u003e\n\u003ctd\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3343-H\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e150 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"FireGene","offers":[{"title":"10 rxns","offer_id":47868399255764,"sku":"FG-BA3343-10","price":699.0,"currency_code":"USD","in_stock":true},{"title":"50 rxns","offer_id":47868399288532,"sku":"FG-BA3343-50","price":2429.0,"currency_code":"USD","in_stock":true}]},{"product_id":"adipose-tissue-nuclei-isolation-kit","title":"FireGene Adipose Nuclei Isolation Kit for Single-Nucleus Sequencing","description":"\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e\u003cstrong\u003eFireGene Adipose Nuclei Isolation Kit \u003c\/strong\u003eis designed for the isolation and extraction of cell nuclei from human or mammalian adipose tissue samples. The kit uses surfactants to disrupt cell membranes and release nuclei while maintaining nuclear membrane integrity. Through tissue grinding, filtration, centrifugation, and density-layer separation, the workflow produces clean single-nucleus suspensions suitable for single-nucleus transcriptome sequencing, single-nucleus ATAC sequencing, and related nuclear analysis experiments.\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eBackground Information\u003c\/h2\u003e\n\u003cp data-end=\"1514\" data-start=\"1244\"\u003eAdipose tissue is lipid-rich and can be difficult to process into high-quality single-cell suspensions. Nuclei isolation provides a practical alternative for adipose samples, especially when working with frozen tissue or samples where whole-cell recovery is challenging.\u003c\/p\u003e\n\u003cp data-end=\"1681\" data-start=\"1516\"\u003e\u003cstrong data-end=\"1557\" data-start=\"1516\"\u003eFireGene Adipose Nuclei Isolation Kit\u003c\/strong\u003e supports preparation of clean single-nucleus suspensions for sequencing-based studies and other nuclear analysis workflows.\u003c\/p\u003e\n\u003ch3 data-end=\"1701\" data-start=\"1683\" data-section-id=\"15x6sgr\"\u003eResearch Areas\u003c\/h3\u003e\n\u003cp data-end=\"1819\" data-start=\"1703\"\u003eThis kit is suitable for research fields focused on adipose biology, metabolic regulation, and tissue heterogeneity.\u003c\/p\u003e\n\u003cul data-end=\"2160\" data-start=\"1821\"\u003e\n\u003cli data-end=\"1845\" data-start=\"1821\" data-section-id=\"j8jxsp\"\u003eAdipose tissue biology\u003c\/li\u003e\n\u003cli data-end=\"1874\" data-start=\"1846\" data-section-id=\"1dtts7f\"\u003eMetabolic disease research\u003c\/li\u003e\n\u003cli data-end=\"1893\" data-start=\"1875\" data-section-id=\"vni08e\"\u003eObesity research\u003c\/li\u003e\n\u003cli data-end=\"1935\" data-start=\"1894\" data-section-id=\"12xwy96\"\u003eDiabetes and insulin resistance studies\u003c\/li\u003e\n\u003cli data-end=\"1976\" data-start=\"1936\" data-section-id=\"uf81qv\"\u003eEndocrine and hormone-related research\u003c\/li\u003e\n\u003cli data-end=\"2017\" data-start=\"1977\" data-section-id=\"1xdu0b1\"\u003eInflammation and immune cell profiling\u003c\/li\u003e\n\u003cli data-end=\"2056\" data-start=\"2018\" data-section-id=\"1lp3159\"\u003eAging-related adipose tissue studies\u003c\/li\u003e\n\u003cli data-end=\"2080\" data-start=\"2057\" data-section-id=\"15qp2sv\"\u003eRegenerative medicine\u003c\/li\u003e\n\u003cli data-end=\"2124\" data-start=\"2081\" data-section-id=\"x5rqu8\"\u003eSingle-cell and single-nucleus multiomics\u003c\/li\u003e\n\u003cli data-end=\"2160\" data-start=\"2125\" data-section-id=\"10aof08\"\u003eTranslational biomedical research\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 data-end=\"2182\" data-start=\"2162\" data-section-id=\"1fuj4ew\"\u003eKey Applications\u003c\/h3\u003e\n\u003cp data-end=\"2290\" data-start=\"2184\"\u003eThe prepared nuclei suspensions can be used in downstream experiments that require clean nuclear material.\u003c\/p\u003e\n\u003cul data-end=\"2658\" data-start=\"2292\"\u003e\n\u003cli data-end=\"2337\" data-start=\"2292\" data-section-id=\"2haltg\"\u003eSingle-nucleus RNA sequencing, or snRNA-seq\u003c\/li\u003e\n\u003cli data-end=\"2385\" data-start=\"2338\" data-section-id=\"9vqous\"\u003eSingle-nucleus ATAC sequencing, or snATAC-seq\u003c\/li\u003e\n\u003cli data-end=\"2418\" data-start=\"2386\" data-section-id=\"1vdl0d5\"\u003eNuclear transcriptome analysis\u003c\/li\u003e\n\u003cli data-end=\"2454\" data-start=\"2419\" data-section-id=\"5n45lt\"\u003eChromatin accessibility profiling\u003c\/li\u003e\n\u003cli data-end=\"2505\" data-start=\"2455\" data-section-id=\"1wvz3b2\"\u003eCell-type composition analysis in adipose tissue\u003c\/li\u003e\n\u003cli data-end=\"2570\" data-start=\"2506\" data-section-id=\"j76kee\"\u003eGene expression profiling from fresh or frozen adipose samples\u003c\/li\u003e\n\u003cli data-end=\"2609\" data-start=\"2571\" data-section-id=\"bpmidh\"\u003eAdipose tissue heterogeneity studies\u003c\/li\u003e\n\u003cli data-end=\"2658\" data-start=\"2610\" data-section-id=\"1fqsuyz\"\u003eDisease mechanism research in metabolic models\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003eSpecifications\u003c\/h2\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eAdipose Nuclei Isolation Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalog No.\u003c\/td\u003e\n\u003ctd\u003eFG-BA3342\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit Size\u003c\/td\u003e\n\u003ctd\u003e10 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Type\u003c\/td\u003e\n\u003ctd\u003eHuman or mammalian adipose tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible Samples\u003c\/td\u003e\n\u003ctd\u003eCell samples, fresh tissue, and frozen tissue samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended Starting Material\u003c\/td\u003e\n\u003ctd\u003eApproximately 200 mg tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Function\u003c\/td\u003e\n\u003ctd\u003eIsolation and extraction of cell nuclei\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWorkflow\u003c\/td\u003e\n\u003ctd\u003eTissue mincing, grinding, lysis, filtration, centrifugation, density-layer separation, nuclei washing and resuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDownstream Applications\u003c\/td\u003e\n\u003ctd\u003eSingle-nucleus transcriptome sequencing, single-nucleus ATAC sequencing, related nuclear analysis experiments\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Instruments\u003c\/td\u003e\n\u003ctd\u003eHorizontal centrifuge, tissue grinder, cell counter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Tissue Tools\u003c\/td\u003e\n\u003ctd\u003eSurgical scissors, scalpels, forceps\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Reagents\u003c\/td\u003e\n\u003ctd\u003eRNase inhibitor for nuclear RNA-related experiments\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Consumables\u003c\/td\u003e\n\u003ctd\u003eGrinding tubes with zirconium beads, 5 mL and 15 mL centrifuge tubes, 40 μm cell strainers, optional 20 μm cell strainers\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProcessing Temperature\u003c\/td\u003e\n\u003ctd\u003eSubsequent procedures after Step 6 should be carried out on wet ice or ice blocks when possible\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eMain reagents: 4°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdditive Storage\u003c\/td\u003e\n\u003ctd\u003eAdditive Solution ① and Additive Solution ②: -20°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf Life\u003c\/td\u003e\n\u003ctd\u003eOne year\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eResearch Use\u003c\/td\u003e\n\u003ctd\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003eKit Components\u003c\/h2\u003e\n\u003ctable style=\"width: 100%; height: 176.344px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003cth style=\"width: 44.2689%; height: 19.5938px;\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"width: 32.5111%; height: 19.5938px;\"\u003eCatalog Number\u003c\/th\u003e\n\u003cth style=\"width: 19.2913%; height: 19.5938px;\" align=\"right\"\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 44.2689%; height: 19.5938px;\"\u003eLysis Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 32.5111%; height: 19.5938px;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3342-A\u003c\/td\u003e\n\u003ctd style=\"width: 19.2913%; height: 19.5938px;\" align=\"right\"\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 44.2689%; height: 19.5938px;\"\u003ePre-Suspension Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 32.5111%; height: 19.5938px;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3342-B\u003c\/td\u003e\n\u003ctd style=\"width: 19.2913%; height: 19.5938px;\" align=\"right\"\u003e15 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 44.2689%; height: 19.5938px;\"\u003ePost-Suspension Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 32.5111%; height: 19.5938px;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3342-C\u003c\/td\u003e\n\u003ctd style=\"width: 19.2913%; height: 19.5938px;\" align=\"right\"\u003e50 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 44.2689%; height: 19.5938px;\"\u003ePB 1\u003c\/td\u003e\n\u003ctd style=\"width: 32.5111%; height: 19.5938px;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3342-D\u003c\/td\u003e\n\u003ctd style=\"width: 19.2913%; height: 19.5938px;\" align=\"right\"\u003e3 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 44.2689%; height: 19.5938px;\"\u003ePB 2\u003c\/td\u003e\n\u003ctd style=\"width: 32.5111%; height: 19.5938px;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3342-E\u003c\/td\u003e\n\u003ctd style=\"width: 19.2913%; height: 19.5938px;\" align=\"right\"\u003e6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 44.2689%; height: 19.5938px;\"\u003ePB 3\u003c\/td\u003e\n\u003ctd style=\"width: 32.5111%; height: 19.5938px;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3342-F\u003c\/td\u003e\n\u003ctd style=\"width: 19.2913%; height: 19.5938px;\" align=\"right\"\u003e6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 44.2689%; height: 19.5938px;\"\u003eAdditive Solution ①\u003c\/td\u003e\n\u003ctd style=\"width: 32.5111%; height: 19.5938px;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3342-G\u003c\/td\u003e\n\u003ctd style=\"width: 19.2913%; height: 19.5938px;\" align=\"right\"\u003e4 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 44.2689%; height: 19.5938px;\"\u003eAdditive Solution ②\u003c\/td\u003e\n\u003ctd style=\"width: 32.5111%; height: 19.5938px;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3342-H\u003c\/td\u003e\n\u003ctd style=\"width: 19.2913%; height: 19.5938px;\" align=\"right\"\u003e150 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"FireGene","offers":[{"title":"10 rxns","offer_id":47868401320148,"sku":"FG-BA3342-10","price":699.0,"currency_code":"USD","in_stock":true},{"title":"50 rxns","offer_id":47868401352916,"sku":"FG-BA3342-50","price":2429.0,"currency_code":"USD","in_stock":true}]}],"url":"https:\/\/firegene.com\/collections\/single-nucleus-rna-sequencing.oembed","provider":"FireGene","version":"1.0","type":"link"}