{"title":"Tissue Dissociation Kits for Single-Cell Sequencing","description":"","products":[{"product_id":"multi-tissue-dissociation-kit-fg-ba3303","title":"FireGene Multi Tissue Dissociation Kit for Single-Cell Suspension","description":"\u003ch3 class=\"\" data-end=\"273\" data-start=\"261\"\u003eOverview\u003c\/h3\u003e\n\u003cp class=\"\" data-end=\"579\" data-start=\"274\"\u003eFireGene’s \u003cstrong data-end=\"318\" data-start=\"285\"\u003eMulti-Tissue Dissociation Kit\u003c\/strong\u003e is engineered for high-efficiency enzymatic tissue dissociation, ideal for generating high-quality single-cell suspensions from a variety of mammalian tissues. It supports applications in single-cell sequencing, disease modeling, and advanced drug development.\u003c\/p\u003e\n\u003ch3 class=\"\" data-end=\"607\" data-start=\"581\"\u003eBackground Information\u003c\/h3\u003e\n\u003cul data-end=\"1367\" data-start=\"608\"\u003e\n\u003cli class=\"\" data-end=\"709\" data-start=\"608\"\u003e\n\u003cp class=\"\" data-end=\"709\" data-start=\"610\"\u003eDesigned to support \u003cstrong data-end=\"708\" data-start=\"630\"\u003esingle-cell research, disease mechanism studies, and therapeutic screening\u003c\/strong\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli class=\"\" data-end=\"790\" data-start=\"710\"\u003e\n\u003cp class=\"\" data-end=\"790\" data-start=\"712\"\u003eEssential in tumor biology, where understanding cellular heterogeneity is key.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli class=\"\" data-end=\"942\" data-start=\"791\"\u003e\n\u003cp class=\"\" data-end=\"818\" data-start=\"793\"\u003eOvercomes limitations of:\u003c\/p\u003e\n\u003cul data-end=\"942\" data-start=\"821\"\u003e\n\u003cli class=\"\" data-end=\"875\" data-start=\"821\"\u003e\n\u003cp class=\"\" data-end=\"875\" data-start=\"823\"\u003e\u003cstrong data-end=\"850\" data-start=\"823\"\u003eMechanical dissociation\u003c\/strong\u003e, which may damage cells.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli class=\"\" data-end=\"942\" data-start=\"878\"\u003e\n\u003cp class=\"\" data-end=\"942\" data-start=\"880\"\u003e\u003cstrong data-end=\"900\" data-start=\"880\"\u003eChemical methods\u003c\/strong\u003e, which risk introducing toxic substances.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli class=\"\" data-end=\"1021\" data-start=\"943\"\u003e\n\u003cp class=\"\" data-end=\"1021\" data-start=\"945\"\u003eDeveloped with deep insight into \u003cstrong data-end=\"1020\" data-start=\"978\"\u003eextracellular matrix-cell interactions\u003c\/strong\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli class=\"\" data-end=\"1241\" data-start=\"1022\"\u003e\n\u003cp class=\"\" data-end=\"1097\" data-start=\"1024\"\u003eCombines \u003cstrong data-end=\"1053\" data-start=\"1033\"\u003emultiple enzymes\u003c\/strong\u003e and a carefully optimized buffer system to:\u003c\/p\u003e\n\u003cul data-end=\"1241\" data-start=\"1100\"\u003e\n\u003cli class=\"\" data-end=\"1164\" data-start=\"1100\"\u003e\n\u003cp class=\"\" data-end=\"1164\" data-start=\"1102\"\u003eAdapt to the diverse structural profiles of different tissues.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli class=\"\" data-end=\"1241\" data-start=\"1167\"\u003e\n\u003cp class=\"\" data-end=\"1241\" data-start=\"1169\"\u003eEnsure consistency and high yield across various experimental workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli class=\"\" data-end=\"1367\" data-start=\"1242\"\u003e\n\u003cp class=\"\" data-end=\"1367\" data-start=\"1244\"\u003eProvides researchers with a \u003cstrong data-end=\"1294\" data-start=\"1272\"\u003euniversal solution\u003c\/strong\u003e for tissue dissociation without compromising cell viability or function.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"\" data-end=\"1392\" data-start=\"1369\"\u003eDetection Principle\u003c\/h3\u003e\n\u003cul data-end=\"1751\" data-start=\"1393\"\u003e\n\u003cli class=\"\" data-end=\"1445\" data-start=\"1393\"\u003e\n\u003cp class=\"\" data-end=\"1445\" data-start=\"1395\"\u003eUtilizes a \u003cstrong data-end=\"1444\" data-start=\"1406\"\u003egentle enzymatic digestion process\u003c\/strong\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli class=\"\" data-end=\"1529\" data-start=\"1446\"\u003e\n\u003cp class=\"\" data-end=\"1529\" data-start=\"1448\"\u003eEfficiently breaks down the extracellular matrix while preserving cell integrity.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli class=\"\" data-end=\"1680\" data-start=\"1530\"\u003e\n\u003cp class=\"\" data-end=\"1541\" data-start=\"1532\"\u003eSupports:\u003c\/p\u003e\n\u003cul data-end=\"1680\" data-start=\"1544\"\u003e\n\u003cli class=\"\" data-end=\"1573\" data-start=\"1544\"\u003e\n\u003cp class=\"\" data-end=\"1573\" data-start=\"1546\"\u003e\u003cstrong data-end=\"1573\" data-start=\"1546\"\u003eRapid tissue processing\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli class=\"\" data-end=\"1605\" data-start=\"1576\"\u003e\n\u003cp class=\"\" data-end=\"1605\" data-start=\"1578\"\u003e\u003cstrong data-end=\"1605\" data-start=\"1578\"\u003eMinimal cellular stress\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli class=\"\" data-end=\"1680\" data-start=\"1608\"\u003e\n\u003cp class=\"\" data-end=\"1680\" data-start=\"1610\"\u003e\u003cstrong data-end=\"1652\" data-start=\"1610\"\u003eHigh-viability single-cell suspensions\u003c\/strong\u003e for downstream applications\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli class=\"\" data-end=\"1751\" data-start=\"1681\"\u003e\n\u003cp class=\"\" data-end=\"1751\" data-start=\"1683\"\u003eCompatible with scRNA-seq, flow cytometry, and primary cell culture.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"\" data-end=\"1392\" data-start=\"1369\"\u003eSpecifications\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eSingle-cell sequencing, cell culture, or other cell-related detections\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eCompatible Sample Types\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eLiver, lung, stomach, intestine, kidney, tumor tissue, mammary gland, ovary, fallopian tube, uterus, testis, epididymis, pituitary gland, thyroid gland, thymus gland, esophagus, nasal tissue, mucous membrane, periosteum, skin, lymphoid tissue, embryonic tissue, and soft horn\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eSupported Instruments\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eWater bath, horizontal centrifuge, cell counter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e-20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eShelf-life\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 class=\"\" data-end=\"1392\" data-start=\"1369\"\u003eKit Components\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e10 reactions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 60%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003e10 Reactions\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003eMTDS\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e2 × 1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e50 reactions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 60%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003e50 Reactions\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003eMTDS\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e10 × 1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProduct FAQ\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e1. Q: Is this kit suitable for all mammalian tissues? Are there any clearly unsuitable tissue types?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: The kit is suitable for various common mammalian tissues, including more than 20 types such as liver, lung, stomach, intestine, kidney, tumor tissue, mammary gland, ovary (as listed in the instruction manual). However, it is not recommended for tissues with extremely high calcification (e.g., mature bone tissue) or excessively high fat content (e.g., omental adipose tissue). The special structure of such tissues may prevent the dissociation solution from acting fully, making it difficult to obtain high-quality single-cell suspensions. If you need to process such tissues, it is recommended to choose a dedicated dissociation kit.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e2.   Q: The mammalian tissue universal dissociation solution (FG-BA3303-A) in the kit components is 2×1.25 mL, and 10 rxns means 10 experiments can be conducted. What volume of dissociation solution is required for each experiment?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Each experiment requires 240 μL of mammalian tissue universal dissociation solution. According to Step 1 of the instruction manual, for each experiment, take one 5 mL centrifuge tube, add 240 μL of dissociation solution and 2760 μL of RPMI 1640 medium, and mix well. The total volume of 2×1.25 mL (2.5 mL in total) of dissociation solution can meet the needs of approximately 10 experiments (2.5 mL = 2500 μL, 2500 μL ÷ 240 μL per experiment ≈ 10.4 experiments). Due to slight losses during operation, it is marked as 10 rxns. If you need to scale up or down the experiment, adjust the volume proportionally.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e3.    Q: During the digestion process, what are the differences between using a water bath and a hybridization oven? Which equipment is better to choose?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: The core difference between the two lies in the mixing method: when using a water bath, the centrifuge tube needs to be shaken manually every 3-5 minutes to ensure full contact between the tissue and the dissociation solution; when using a hybridization oven, a rotation speed of 20-30 rpm can be set to achieve automatic mixing, reducing manual operation errors. There is no absolute \"better\" choice, and it needs to be based on experimental conditions: if the number of experimental samples is large and the operator's time is limited, the automatic mixing of the hybridization oven is more efficient, and it can avoid digestion differences caused by uneven manual shaking strength; if the number of samples is small, manual operation with a water bath can meet the needs. Both types of equipment need to maintain a constant temperature of 37°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e4.    Q: The instruction manual mentions that \"different types and states of tissues have different digestion times, and the cell suspension should be quality-checked at regular intervals\". How often should the quality check be conducted specifically? What are the core indicators of quality check?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: It is recommended to conduct the first quality check after 15 minutes of digestion, and then conduct quality checks every 20-30 minutes thereafter. The core indicators of quality check include two aspects: first, cell viability; second, cell dispersion. Take a small amount of suspension for microscopic examination to observe whether there are obvious tissue clumps (if the proportion of tissue clumps is significant, the digestion time can be appropriately extended, but cell viability must be monitored simultaneously to avoid over-digestion).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e5.    Q: In Steps 5-7, after digestion, it is necessary to filter with a 70 μm cell sieve and wash the centrifuge tube 3 times. Will omitting 1-2 washing steps affect the experimental results?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: It will significantly affect the results. Omitting washing steps will result in residual dissociation solution, tissue debris, and dead cells not being completely removed: on the one hand, the residual dissociation solution may continue to damage the activity of subsequent cells, especially having a great impact on the cell state in subsequent cell culture or single-cell sequencing; on the other hand, residual impurities will interfere with the quality control results of the cell counter, leading to inaccurate calculation of cell concentration. Therefore, it is necessary to strictly perform 3 washing steps to ensure that a total of 12 mL of filtrate is collected and impurities are fully removed.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e6.    Q: Steps 9 and 10 mention washing the precipitate twice with PBS containing 5% FBS. What is the function of FBS? Can it be replaced with PBS without FBS?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The core function of FBS (fetal bovine serum) is to neutralize the activity of residual dissociation solution, prevent the dissociation solution from continuously damaging cells, and at the same time provide basic nutrients for cells to maintain cell viability. It cannot be replaced with PBS without FBS. If PBS without FBS is used, the residual dissociation solution will continue to damage the cell membrane, leading to a significant decrease in subsequent cell viability. Especially for samples that need to undergo cell culture or single-cell sequencing, this may directly lead to experimental failure.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e7.    Q: The instruction manual notes that \"DMEM medium can replace RPMI 1640 medium\". There are differences in their components. Is it necessary to adjust the experimental steps or reagent dosage after replacement?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: There is no need to adjust the experimental steps or reagent dosage. Both DMEM and RPMI 1640 media are common basic media for mammalian cells. Although there are slight differences in the contents of amino acids, vitamins and other components, both can provide a suitable osmotic pressure and pH environment for tissue dissociation and meet the working conditions of the dissociation solution. When replacing, add it directly according to the original dosage (2760 μL per experiment), which has no significant impact on the dissociation effect and cell viability.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e8.    Q: If red blood cell removal is required, the \u003ca href=\"https:\/\/firegene.com\/products\/red-blood-cell-lysis-kit-fg-ba3311?_pos=4\u0026amp;_sid=e8a67b419\u0026amp;_ss=r\"\u003ered blood cell lysis kit (FG-BA3311)\u003c\/a\u003e should be used. At which step should this kit be added for operation? What specific points should be noted?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: It is recommended to perform the red blood cell removal operation after Step 10 and before Step 11. The specific process is: after completing the two PBS washes in Step 10 and discarding the supernatant, add the lysis solution from the red blood cell lysis kit (according to the dosage in the instruction manual of that kit).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e9.    Q: The kit should be stored at -20°C. If it is accidentally thawed during transportation or before the experiment (e.g., left at room temperature for 1 hour), can the thawed dissociation solution still be used?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: If it is only thawed briefly (within 1 hour at room temperature and without repeated freezing and thawing), mix it well immediately after thawing and store it back at -20°C, and it can still be used; if the thawing time exceeds 2 hours, or there is repeated freezing and thawing (frozen again after thawing, then thawed again), it cannot be used. The enzyme activity in the dissociation solution is sensitive to temperature. Prolonged storage at room temperature or repeated freezing and thawing will lead to a decrease in enzyme activity and dissociation efficiency, and may result in insufficient tissue digestion. If the thawed dissociation solution has been used, the dissociation effect should be evaluated by shortening the quality check interval and closely monitoring cell viability.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e10.    Q: Step 13 states that \"subsequent experiments should be carried out immediately after quality control\". If subsequent experiments cannot be carried out immediately, can the prepared single-cell suspension be stored for a short period? What are the storage conditions?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Short-term storage is possible, but the storage time should not exceed 1 hour. The storage condition is a 4°C refrigerator, and repeated shaking should be avoided. During storage, the cell suspension should be adjusted to an appropriate concentration (1×10⁶ - 1×10⁷ cells\/mL) with PBS containing 5% FBS, and placed in a sterile low-adhesion centrifuge tube for sealing. It should be noted that if the storage time exceeds 1 hour, cell viability will gradually decrease, especially for single-cell sequencing samples, which may affect the subsequent gene expression detection results; if the suspension needs to be used after storage, it is necessary to re-conduct the cell viability quality check and gently pipette to mix well to avoid cell precipitation.\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"2 reactions\/kit","offer_id":47833302761684,"sku":"FG-BA3303-2rxns","price":79.0,"currency_code":"USD","in_stock":true},{"title":"10 reactions\/kit","offer_id":46299523547348,"sku":"FG-BA3303-10rxns","price":309.0,"currency_code":"USD","in_stock":true},{"title":"50 reactions\/kit","offer_id":47683128721620,"sku":"FG-BA3303-50rxns","price":1289.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/files\/BA3303.png?v=1773828041"},{"product_id":"bone-dissociation-kit-fg-ba3308","title":"FireGene Bone Dissociation Kit - Enzymatic Single-Cell Prep","description":"\u003ch3 class=\"\" data-end=\"273\" data-start=\"259\"\u003eOverview\u003c\/h3\u003e\n\u003cp class=\"\" data-end=\"569\" data-start=\"274\"\u003eThe \u003cstrong data-end=\"312\" data-start=\"278\"\u003eFireGene Bone Dissociation Kit\u003c\/strong\u003e is a powerful enzymatic solution designed to generate high-quality single-cell suspensions from bone tissue. It is ideal for applications in bone disease research, regenerative medicine, and stem cell studies, offering consistent and viable cell isolation.\u003c\/p\u003e\n\u003chr class=\"\" data-end=\"574\" data-start=\"571\"\u003e\n\u003ch3 class=\"\" data-end=\"602\" data-start=\"576\"\u003eBackground Information\u003c\/h3\u003e\n\u003cul data-end=\"1635\" data-start=\"604\"\u003e\n\u003cli class=\"\" data-end=\"1200\" data-start=\"604\"\u003e\n\u003cp class=\"\" data-end=\"659\" data-start=\"606\"\u003e\u003cstrong data-end=\"659\" data-start=\"606\"\u003eDriven by Clinical and Scientific Research Needs:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul data-end=\"1200\" data-start=\"662\"\u003e\n\u003cli class=\"\" data-end=\"758\" data-start=\"662\"\u003e\n\u003cp class=\"\" data-end=\"758\" data-start=\"664\"\u003eBone tissue research is essential for studying diseases like \u003cstrong data-end=\"757\" data-start=\"725\"\u003eosteoporosis and bone tumors\u003c\/strong\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli class=\"\" data-end=\"867\" data-start=\"761\"\u003e\n\u003cp class=\"\" data-end=\"867\" data-start=\"763\"\u003eSingle-cell level analysis allows for better understanding of \u003cstrong data-end=\"851\" data-start=\"825\"\u003ecellular heterogeneity\u003c\/strong\u003e in bone tissue.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli class=\"\" data-end=\"962\" data-start=\"870\"\u003e\n\u003cp class=\"\" data-end=\"962\" data-start=\"872\"\u003eConventional tissue sectioning methods are insufficient for detailed cellular exploration.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli class=\"\" data-end=\"1091\" data-start=\"965\"\u003e\n\u003cp class=\"\" data-end=\"1091\" data-start=\"967\"\u003eIn regenerative medicine, isolating viable \u003cstrong data-end=\"1055\" data-start=\"1010\"\u003ebone cells (e.g., mesenchymal stem cells)\u003c\/strong\u003e is critical for tissue engineering.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli class=\"\" data-end=\"1200\" data-start=\"1094\"\u003e\n\u003cp class=\"\" data-end=\"1200\" data-start=\"1096\"\u003eHigh-quality single-cell suspensions support \u003cstrong data-end=\"1174\" data-start=\"1141\"\u003ecell culture, differentiation\u003c\/strong\u003e, and downstream analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli class=\"\" data-end=\"1635\" data-start=\"1202\"\u003e\n\u003cp class=\"\" data-end=\"1248\" data-start=\"1204\"\u003e\u003cstrong data-end=\"1248\" data-start=\"1204\"\u003eBackground of Technological Development:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul data-end=\"1635\" data-start=\"1251\"\u003e\n\u003cli class=\"\" data-end=\"1336\" data-start=\"1251\"\u003e\n\u003cp class=\"\" data-end=\"1336\" data-start=\"1253\"\u003eTraditional dissociation methods often lead to \u003cstrong data-end=\"1335\" data-start=\"1300\"\u003ecell damage and poor efficiency\u003c\/strong\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli class=\"\" data-end=\"1635\" data-start=\"1339\"\u003e\n\u003cp class=\"\" data-end=\"1399\" data-start=\"1341\"\u003eThis kit leverages \u003cstrong data-end=\"1393\" data-start=\"1360\"\u003eadvanced enzymatic techniques\u003c\/strong\u003e with:\u003c\/p\u003e\n\u003cul data-end=\"1635\" data-start=\"1404\"\u003e\n\u003cli class=\"\" data-end=\"1467\" data-start=\"1404\"\u003e\n\u003cp class=\"\" data-end=\"1467\" data-start=\"1406\"\u003ePrecisely selected enzymes tailored to bone matrix breakdown.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli class=\"\" data-end=\"1530\" data-start=\"1472\"\u003e\n\u003cp class=\"\" data-end=\"1530\" data-start=\"1474\"\u003eOptimized enzyme concentrations and reaction conditions.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli class=\"\" data-end=\"1635\" data-start=\"1535\"\u003e\n\u003cp class=\"\" data-end=\"1635\" data-start=\"1537\"\u003eImproved dissociation efficiency and \u003cstrong data-end=\"1601\" data-start=\"1574\"\u003eenhanced cell viability\u003c\/strong\u003e for experimental reproducibility.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr class=\"\" data-end=\"1640\" data-start=\"1637\"\u003e\n\u003ch3 class=\"\" data-end=\"1665\" data-start=\"1642\"\u003eDetection Principle\u003c\/h3\u003e\n\u003cul data-end=\"2110\" data-start=\"1667\"\u003e\n\u003cli class=\"\" data-end=\"1770\" data-start=\"1667\"\u003e\n\u003cp class=\"\" data-end=\"1770\" data-start=\"1669\"\u003eUses a \u003cstrong data-end=\"1715\" data-start=\"1676\"\u003etwo-step enzymatic digestion method\u003c\/strong\u003e tailored to the density and complexity of bone tissue.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli class=\"\" data-end=\"1992\" data-start=\"1771\"\u003e\n\u003cp class=\"\" data-end=\"1781\" data-start=\"1773\"\u003eProcess:\u003c\/p\u003e\n\u003cul data-end=\"1992\" data-start=\"1784\"\u003e\n\u003cli class=\"\" data-end=\"1827\" data-start=\"1784\"\u003e\n\u003cp class=\"\" data-end=\"1827\" data-start=\"1786\"\u003eBone tissue is \u003cstrong data-end=\"1826\" data-start=\"1801\"\u003ecut into small pieces\u003c\/strong\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli class=\"\" data-end=\"1907\" data-start=\"1830\"\u003e\n\u003cp class=\"\" data-end=\"1907\" data-start=\"1832\"\u003e\u003cstrong data-end=\"1855\" data-start=\"1832\"\u003eTwo enzyme mixtures\u003c\/strong\u003e are added successively under controlled conditions.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli class=\"\" data-end=\"1992\" data-start=\"1910\"\u003e\n\u003cp class=\"\" data-end=\"1992\" data-start=\"1912\"\u003eEnzymes break down the \u003cstrong data-end=\"1959\" data-start=\"1935\"\u003eextracellular matrix\u003c\/strong\u003e and \u003cstrong data-end=\"1991\" data-start=\"1964\"\u003eintercellular junctions\u003c\/strong\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli class=\"\" data-end=\"2110\" data-start=\"1993\"\u003e\n\u003cp class=\"\" data-end=\"2006\" data-start=\"1995\"\u003eThe result:\u003c\/p\u003e\n\u003cul data-end=\"2110\" data-start=\"2009\"\u003e\n\u003cli class=\"\" data-end=\"2110\" data-start=\"2009\"\u003e\n\u003cp class=\"\" data-end=\"2110\" data-start=\"2011\"\u003e\u003cstrong data-end=\"2052\" data-start=\"2011\"\u003eClean, viable single-cell suspensions\u003c\/strong\u003e suitable for flow cytometry, scRNA-seq, and cell culture.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"\" data-end=\"1665\" data-start=\"1642\"\u003eSpecifications\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eSingle-cell sequencing, cell culture or other cell-related detections\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eCompatible Sample Types\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eBone tissue, including cartilage and bone\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eSupported Instruments\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eWater bath, horizontal centrifuge, cell counter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e-20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eShelf-life\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 class=\"\" data-end=\"1665\" data-start=\"1642\"\u003eKit Components\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e10 reactions\/kit\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"table-wrapper\"\u003e\n\u003ctable height=\"150\" style=\"width: 100%; height: 128px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003cth style=\"width: 56.6258%; height: 19.5938px;\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"width: 43.0132%; height: 19.5938px;\"\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 54.2031px;\"\u003e\n\u003ctd style=\"width: 56.6258%; height: 54.2031px;\"\u003e\u003cspan\u003eBDS1 (Bone Dissociation Solution 1)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 43.0132%; height: 54.2031px;\"\u003e\n\u003cspan\u003e2 \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Times New Roman;\"\u003e×\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e 1.1\u003c\/span\u003e\u003cspan\u003e m\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 54.2031px;\"\u003e\n\u003ctd style=\"width: 56.6258%; height: 54.2031px;\"\u003e\u003cspan\u003eBDS2 (Bone Dissociation Solution 2)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 43.0132%; height: 54.2031px;\"\u003e\n\u003cspan\u003e2 \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Times New Roman;\"\u003e×\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e 1.4\u003c\/span\u003e\u003cspan\u003e m\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e50 reactions\/kit\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"table-wrapper\"\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"width: 56.8669%;\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"width: 42.772%;\"\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 56.8669%;\"\u003e\u003cspan\u003eBDS1 (Bone Dissociation Solution 1)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 42.772%;\"\u003e\n\u003cspan\u003e10 \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Times New Roman;\"\u003e×\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e 1.1\u003c\/span\u003e\u003cspan\u003e m\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 56.8669%;\"\u003e\u003cspan\u003eBDS2 (Bone Dissociation Solution 2)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 42.772%;\"\u003e\n\u003cspan\u003e10 \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Times New Roman;\"\u003e×\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e 1.4\u003c\/span\u003e\u003cspan\u003e m\u003c\/span\u003e\u003cspan\u003eL\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch4\u003e\u003cbr\u003e\u003c\/h4\u003e\n\u003ch4\u003eProduct Q\u0026amp;A\u003c\/h4\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e1. Q: Is this kit suitable for all types of bone tissue? Do the operation steps need to be adjusted for the dissociation of cartilage and hard bone?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: The kit is suitable for cartilage and hard bone tissue of mammals such as humans and mice. However, since hard bone has a higher degree of mineralization, two points should be noted during dissociation: first, the chopping step needs to be more thorough (chop into small pieces of 1mm³ or less as much as possible) to ensure the dissociation solution fully contacts the tissue; second, the incubation time of Dissociation Solution 2 can be appropriately extended (8-14 hours recommended for hard bone, 6-10 hours for cartilage), and specific adjustments should be made based on quality inspection results. No other operation steps need to be changed; only the chopping degree and incubation time are optimized to adapt to different types of bone tissue.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e2. Q: The kit is labeled \"10 tests\". What are the respective dosages of Bone Tissue Dissociation Solution 1 and Dissociation Solution 2 per experiment? If only 100mg of bone tissue needs to be processed in a single experiment, can the reagent dosage be reduced proportionally?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: For each experiment (200mg of tissue), 210μL of Bone Tissue Dissociation Solution 1 and 280μL of Bone Tissue Dissociation Solution 2 are required. If processing 100mg of tissue (1\/2 of the standard dosage), the reagent dosage can be reduced proportionally: Dissociation Solution 1 reduced to 105μL (mixed with 1395μL of RPMI 1640 medium), and Dissociation Solution 2 reduced to 140μL (mixed with 1360μL of RPMI 1640 medium). Note: The reagent volume must ensure pipetting accuracy (it is recommended that a single pipetting volume is not less than 50μL). If the tissue amount is less than 50mg, it is not recommended to further reduce the dosage proportionally, to avoid pipetting errors caused by too small reagent volume and thus affecting the dissociation effect.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e3. Q: Both Step 3 and the incubation of Dissociation Solution 2 mention \"37°C water bath or hybridization oven\". What are the differences in operation and effect between the two devices? Which one is more suitable for bone tissue dissociation?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: The core difference lies in the mixing method: a water bath requires manual shaking of the centrifuge tube every 3-5 minutes to ensure the tissue contacts the dissociation solution; a hybridization oven can be set to 20-30 rpm for automatic mixing, reducing manual operation errors. In terms of effect, the hybridization oven is more suitable for bone tissue dissociation. Bone tissue has a hard texture, and manual shaking is prone to uneven local contact, leading to incomplete dissociation; automatic mixing allows the dissociation solution to continuously wrap the tissue, which can significantly improve the single-cell yield especially during the long-term incubation (6-14 hours) of Dissociation Solution 2. If a water bath is used, the 3-5 minute shaking frequency must be strictly followed to avoid omissions.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e4. Q: Step 7 mentions \"quality inspection at regular intervals\". What are the quality inspection intervals and judgment criteria for bone tissue dissociation? If cell viability is low but the tissue is not fully dissociated during quality inspection, how to handle it?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: Recommended quality inspection intervals: in the first 4 hours of Dissociation Solution 2 incubation, conduct quality inspection once every 1 hour; after 4 hours, conduct it once every 2 hours. Quality inspection operation: take 10μL of the suspension, stain with trypan blue, and observe under a microscope. The core judgment criteria are cell viability ≥70% and tissue clump proportion ≤10%. If cell viability is low (\u0026lt;60%) but there are still tissue clumps, digestion must be stopped immediately. In bone tissue dissociation, the damage to cell viability caused by excessive enzymolysis is irreversible; continued incubation will lead to a further decrease in viability. Residual tissue clumps can be removed by filtration in subsequent steps, and complete dissociation is not necessary.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e5. Q: Steps 8-10 require filtration with a 70μm cell sieve and rinsing the centrifuge tube 3 times, collecting a total of 12mL of filtrate. What impact will omitting one rinsing step have on the experimental results? Can a 100μm cell sieve replace the 70μm one?\u003c\/strong\u003e\u003c\/span\u003e\u003cspan\u003eA: Omitting one rinsing step will result in the loss of approximately 1\/3 of residual cells, reducing the final cell yield by 25%-30%. Cells are prone to adhering to the inner wall of the centrifuge tube after bone tissue dissociation, so 3 rinsing steps are the key to ensuring full cell recovery. A 100μm cell sieve cannot replace the 70μm one: the pore size of the 100μm sieve is too large to effectively filter fine debris (such as mineralized particles and fiber residues) remaining in bone tissue. These impurities will interfere with subsequent cell counting and even block single-cell sequencing chips, affecting experimental results.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e6. Q: The instruction manual states that \"DMEM medium can replace RPMI 1640 medium\". After replacement, is it necessary to adjust the reagent dosage or incubation time? Do the two media have an impact on the cell viability of bone tissue dissociation?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: No adjustment to reagent dosage or incubation time is needed after replacement. Both DMEM and RPMI 1640 are common basal media for mammalian cells. Although they differ in amino acid and vitamin content, both can provide a suitable osmotic pressure and pH environment for bone tissue dissociation, with no impact on dissociation efficiency. The impact on cell viability is minimal: experimental data shows that the difference in cell viability after dissociating mouse femur tissue with the two media is ≤4%. The medium can be selected based on the existing inventory in the laboratory, with no need for deliberate replacement.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e7. Q: If there are many red blood cells in the cell suspension after dissociation and FG-BA3311 Red Blood Cell Lysis Buffer is required for removal, at which step should this operation be performed? What precautions should be taken during lysis?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: The operation should be performed after Step 13 and before Step 14: after completing the two washing steps in Step 13 and discarding the supernatant, add 1mL of FG-BA3311 Red Blood Cell Lysis Buffer, incubate at room temperature for 5 minutes, centrifuge at 300×g for 5 minutes at 4°C, discard the supernatant, and resuspend the pellet with 5mL of PBS containing 5% FBS (adding an extra washing step) before proceeding to Step 14. Precautions: The lysis time should not be too long, otherwise it will damage bone tissue-derived cells (such as osteoblasts and osteoclasts); if there are too many red blood cells, lysis can be repeated once, but an additional washing step is required to avoid residual lysis buffer affecting subsequent experiments.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e8. Q: The kit needs to be stored at -20°C. If the ice pack melts during transportation and the reagent is left at 4°C for 3 hours, can it still be used? What are the impacts of repeated freezing and thawing?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: The reagent can still be used if left at 4°C for 1 hour, but it should be immediately returned to -20°C and fully mixed before subsequent use. The enzyme in the dissociation solution loses ≤10% of its activity when placed at 4°C for a short time (≤1 hour), which does not affect the dissociation effect. Repeated freezing and thawing will lead to a significant decrease in enzyme activity: each freeze-thaw cycle reduces enzyme activity by 12%-18%; after more than 3 cycles, the activity is less than 50%, making it unable to effectively decompose the extracellular matrix of bone tissue and resulting in incomplete dissociation. It is recommended that after receiving the kit, aliquot Dissociation Solution 1 and Dissociation Solution 2 into single-use volumes (e.g., 210μL\/tube for Dissociation Solution 1, 280μL\/tube for Dissociation Solution 2), seal them, store at -20°C, and take one tube per experiment to avoid repeated freezing and thawing.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e9. Q: During bone tissue dissociation, if obvious tissue clumps are still found in the pellet after centrifugation in Step 4, is it necessary to add Dissociation Solution 1 again for re-digestion? Will it affect the subsequent incubation effect of Dissociation Solution 2?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: There is no need to add Dissociation Solution 1 again for re-digestion. The purpose of centrifugation in Step 4 is to remove Dissociation Solution 1 and residual impurities. If there are tissue clumps at this time, after adding Dissociation Solution 2 in Step 6, the dissociation of tissue clumps can be promoted by extending the incubation time (e.g., extending from 8 hours to 10 hours for hard bone) or increasing the mixing frequency during incubation (the rotation speed of the hybridization oven can be slightly adjusted to 30 rpm). Re-adding Dissociation Solution 1 will lead to excessive enzyme concentration, which, when combined with Dissociation Solution 2 in subsequent steps, is likely to cause over-digestion of cells and a significant decrease in viability, thereby adversely affecting the experimental results.\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"2 reactions\/kit","offer_id":47833307250900,"sku":"FG-BA3308-2rxns","price":119.0,"currency_code":"USD","in_stock":true},{"title":"10 reactions\/kit","offer_id":46299523580116,"sku":"FG-BA3308-10rxns","price":499.0,"currency_code":"USD","in_stock":true},{"title":"50 reactions\/kit","offer_id":47678026088660,"sku":"FG-BA3308-50rxns","price":1749.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/files\/BA3308_1c601e31-39a0-4146-9bc8-58bae5ed8d17.png?v=1773915302"},{"product_id":"mouse-brain-dissociation-kit-fg-ba3305","title":"FireGene Mouse Brain Dissociation Kit for scRNA-seq","description":"\u003ch3 data-start=\"277\" data-end=\"291\" class=\"\"\u003eOverview\u003c\/h3\u003e\n\u003cp data-start=\"292\" data-end=\"598\" class=\"\"\u003eThe \u003cstrong data-start=\"296\" data-end=\"337\"\u003eFireGene Mouse Brain Dissociation Kit\u003c\/strong\u003e is engineered to produce high-quality single-cell suspensions from mouse brain tissue using an optimized enzymatic process. This kit is ideal for neuroscience research, enabling precise gene expression profiling and functional studies at the single-cell level.\u003c\/p\u003e\n\u003chr data-start=\"600\" data-end=\"603\" class=\"\"\u003e\n\u003ch3 data-start=\"605\" data-end=\"631\" class=\"\"\u003eBackground Information\u003c\/h3\u003e\n\u003cul data-start=\"633\" data-end=\"1647\"\u003e\n\u003cli data-start=\"633\" data-end=\"1242\" class=\"\"\u003e\n\u003cp data-start=\"635\" data-end=\"688\" class=\"\"\u003e\u003cstrong data-start=\"635\" data-end=\"688\"\u003eDriven by Clinical and Scientific Research Needs:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul data-start=\"691\" data-end=\"1242\"\u003e\n\u003cli data-start=\"691\" data-end=\"781\" class=\"\"\u003e\n\u003cp data-start=\"693\" data-end=\"781\" class=\"\"\u003eIncreased demand for high-resolution studies of \u003cstrong data-start=\"741\" data-end=\"780\"\u003ecellular heterogeneity in the brain\u003c\/strong\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"784\" data-end=\"885\" class=\"\"\u003eTraditional methods fail to preserve delicate brain cell populations and often yield low viability.\u003c\/li\u003e\n\u003cli data-start=\"888\" data-end=\"1145\" class=\"\"\u003e\n\u003cp data-start=\"890\" data-end=\"943\" class=\"\"\u003eSingle-cell sequencing of mouse brain tissue enables:\u003c\/p\u003e\n\u003cul data-start=\"948\" data-end=\"1145\"\u003e\n\u003cli data-start=\"948\" data-end=\"984\" class=\"\"\u003e\n\u003cp data-start=\"950\" data-end=\"984\" class=\"\"\u003eDetailed gene expression analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"989\" data-end=\"1066\" class=\"\"\u003e\n\u003cp data-start=\"991\" data-end=\"1066\" class=\"\"\u003eFunctional characterization of \u003cstrong data-start=\"1022\" data-end=\"1065\"\u003eneurons, glia, and other CNS cell types\u003c\/strong\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1071\" data-end=\"1145\" class=\"\"\u003e\n\u003cp data-start=\"1073\" data-end=\"1145\" class=\"\"\u003eDiscovery of new biomarkers and therapeutic targets for brain disorders.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1148\" data-end=\"1242\" class=\"\"\u003e\n\u003cp data-start=\"1150\" data-end=\"1242\" class=\"\"\u003eCritical for exploring \u003cstrong data-start=\"1173\" data-end=\"1230\"\u003eneurodevelopment, neurodegeneration, and brain repair\u003c\/strong\u003e mechanisms.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1244\" data-end=\"1647\" class=\"\"\u003e\n\u003cp data-start=\"1246\" data-end=\"1290\" class=\"\"\u003e\u003cstrong data-start=\"1246\" data-end=\"1290\"\u003eBackground of Technological Development:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul data-start=\"1293\" data-end=\"1647\"\u003e\n\u003cli data-start=\"1293\" data-end=\"1365\" class=\"\"\u003e\n\u003cp data-start=\"1295\" data-end=\"1365\" class=\"\"\u003eOvercomes limitations of mechanical and chemical dissociation methods.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1368\" data-end=\"1572\" class=\"\"\u003e\n\u003cp data-start=\"1370\" data-end=\"1416\" class=\"\"\u003eEmploys \u003cstrong data-start=\"1378\" data-end=\"1410\"\u003eadvanced enzymatic protocols\u003c\/strong\u003e that:\u003c\/p\u003e\n\u003cul data-start=\"1421\" data-end=\"1572\"\u003e\n\u003cli data-start=\"1421\" data-end=\"1480\" class=\"\"\u003e\n\u003cp data-start=\"1423\" data-end=\"1480\" class=\"\"\u003eUse optimized enzyme concentrations and incubation times.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1485\" data-end=\"1527\" class=\"\"\u003e\n\u003cp data-start=\"1487\" data-end=\"1527\" class=\"\"\u003eMinimize damage to fragile neural cells.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1532\" data-end=\"1572\" class=\"\"\u003e\n\u003cp data-start=\"1534\" data-end=\"1572\" class=\"\"\u003ePreserve cell viability and diversity.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1575\" data-end=\"1647\" class=\"\"\u003e\n\u003cp data-start=\"1577\" data-end=\"1647\" class=\"\"\u003eDesigned to ensure \u003cstrong data-start=\"1596\" data-end=\"1646\"\u003econsistent, reproducible dissociation outcomes\u003c\/strong\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"1649\" data-end=\"1652\" class=\"\"\u003e\n\u003ch3 data-start=\"1654\" data-end=\"1677\" class=\"\"\u003eDetection Principle\u003c\/h3\u003e\n\u003cul data-start=\"1679\" data-end=\"2240\"\u003e\n\u003cli data-start=\"1679\" data-end=\"1762\" class=\"\"\u003e\n\u003cp data-start=\"1681\" data-end=\"1762\" class=\"\"\u003eUtilizes a \u003cstrong data-start=\"1692\" data-end=\"1734\"\u003esynergistic enzymatic digestion method\u003c\/strong\u003e optimized for brain tissue.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1763\" data-end=\"2107\" class=\"\"\u003e\n\u003cp data-start=\"1765\" data-end=\"1784\" class=\"\"\u003eProcess highlights:\u003c\/p\u003e\n\u003cul data-start=\"1787\" data-end=\"2107\"\u003e\n\u003cli data-start=\"1787\" data-end=\"1858\" class=\"\"\u003e\n\u003cp data-start=\"1789\" data-end=\"1858\" class=\"\"\u003eMouse brain tissue is \u003cstrong data-start=\"1811\" data-end=\"1836\"\u003ecut into small pieces\u003c\/strong\u003e for uniform exposure.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1861\" data-end=\"1942\" class=\"\"\u003e\n\u003cp data-start=\"1863\" data-end=\"1942\" class=\"\"\u003e\u003cstrong data-start=\"1863\" data-end=\"1905\"\u003eSequential addition of enzyme mixtures\u003c\/strong\u003e facilitates gentle tissue breakdown.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1945\" data-end=\"2026\" class=\"\"\u003e\n\u003cp data-start=\"1947\" data-end=\"2026\" class=\"\"\u003eIncubation is performed under \u003cstrong data-start=\"1977\" data-end=\"2025\"\u003econtrolled temperature and timing conditions\u003c\/strong\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2029\" data-end=\"2107\" class=\"\"\u003e\n\u003cp data-start=\"2031\" data-end=\"2107\" class=\"\"\u003eEnzymes selectively degrade the \u003cstrong data-start=\"2063\" data-end=\"2106\"\u003eextracellular matrix and cell junctions\u003c\/strong\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2108\" data-end=\"2240\" class=\"\"\u003e\n\u003cp data-start=\"2110\" data-end=\"2123\" class=\"\"\u003eFinal output:\u003c\/p\u003e\n\u003cul data-start=\"2126\" data-end=\"2240\"\u003e\n\u003cli data-start=\"2126\" data-end=\"2240\" class=\"\"\u003e\n\u003cp data-start=\"2128\" data-end=\"2240\" class=\"\"\u003eA \u003cstrong data-start=\"2130\" data-end=\"2178\"\u003eclean, high-viability single-cell suspension\u003c\/strong\u003e ready for scRNA-seq, flow cytometry, and downstream analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 data-start=\"1654\" data-end=\"1677\" class=\"\"\u003eSpecifications\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eSingle-cell sequencing, cell culture or other cell-related detections\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eCompatible Sample Types\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eMouse brain tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eSupported Instruments\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eWater bath, horizontal centrifuge, cell counter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e-20 °C \/ 4 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eShelf-life\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e24 months at -20 °C\u003cbr\u003e12 months at 4 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 data-start=\"1654\" data-end=\"1677\" class=\"\"\u003eKit Components\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e10 reactions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"width: 25%; padding: 8px; border: 1px solid #ddd; background: #f0f0f0;\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"width: 25%; padding: 8px; border: 1px solid #ddd; background: #f0f0f0;\"\u003e10 Tests\/Kit\u003c\/th\u003e\n\u003cth style=\"width: 25%; padding: 8px; border: 1px solid #ddd; background: #f0f0f0;\"\u003eStorage\u003c\/th\u003e\n\u003cth style=\"width: 25%; padding: 8px; border: 1px solid #ddd; background: #f0f0f0;\"\u003eShelf-life\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003eBuffer A\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e1*21.6 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e-20 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003eEnzyme B\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e1*8 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e-20 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003eEnzyme C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e1*100 μL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e4 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003eDRS\u003cspan\u003e(Mouse Brain Cell Debris Removal Buffer)\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e1*10 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e4 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e50 reactions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"width: 25%; padding: 8px; border: 1px solid #ddd; background: #f0f0f0;\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"width: 25%; padding: 8px; border: 1px solid #ddd; background: #f0f0f0;\"\u003e10 Tests\/Kit\u003c\/th\u003e\n\u003cth style=\"width: 25%; padding: 8px; border: 1px solid #ddd; background: #f0f0f0;\"\u003eStorage\u003c\/th\u003e\n\u003cth style=\"width: 25%; padding: 8px; border: 1px solid #ddd; background: #f0f0f0;\"\u003eShelf-life\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003eBuffer A\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e5*21.6 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e-20 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003eEnzyme B\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e5*8 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e-20 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003eEnzyme C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e5*100 μL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e4 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003eDRS\u003cspan\u003e(Mouse Brain Cell Debris Removal Buffer)\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e5*10 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e4 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch4\u003e\u003c\/h4\u003e\n\u003ch4\u003eProduct Q\u0026amp;A\u003c\/h4\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e1. Q: Is this kit only suitable for mouse brain tissue? Is it applicable to brain tissue of other animals such as rats and rabbits? Can it be used for non-brain tissues of mice (e.g., liver, kidney)?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eA: This kit is specially designed for mouse brain tissue and is only suitable for the dissociation of mouse brain tissue. It is not recommended for use with brain tissue of other animals like rats and rabbits for the time being. The cell density and extracellular matrix composition of brain tissue vary among different animals, and the enzyme ratio and buffer components in the kit are optimized for mouse brain tissue. Using it on other animals may result in low dissociation efficiency or poor cell viability. Meanwhile, it cannot be used for non-brain tissues of mice. Non-brain tissues (such as liver and kidney) have significantly different structures from brain tissue, so a dedicated dissociation kit for the corresponding tissue (e.g., \u003ca href=\"https:\/\/firegene.com\/products\/liver-dissociation-kit-fg-ba3323?_pos=1\u0026amp;_sid=f8e64b327\u0026amp;_ss=r\"\u003eFG-BA3323 Liver Tissue Dissociation Kit\u003c\/a\u003e) should be used.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e2. Q: The components of the kit have different storage conditions. What should be noted before mixing and using them? If Enzyme C is accidentally stored frozen at -20°C, can it still be used?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: Two points should be noted before mixing and using: First, confirm that all components are within their validity period and stored in compliance with the required conditions (Buffer A and Enzyme B at -20°C; Enzyme C and DRS at 4°C in the dark). Second, before use, take Buffer A and Enzyme B out of the -20°C freezer, thaw them at room temperature, and mix thoroughly. Enzyme C and DRS can be directly taken out of the 4°C refrigerator and mixed well, with repeated freezing and thawing avoided. If Enzyme C is accidentally stored frozen at -20°C, it cannot be used anymore. Enzyme C is an enzyme preparation, and low-temperature freezing will destroy its spatial structure, leading to complete loss of enzyme activity. Using it will fail to dissociate the tissue effectively, and you need to contact the manufacturer to purchase a replacement.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e3. Q: Step 1 requires cutting about 200mg of fresh tissue. What impact will insufficient tissue quantity (e.g., only 50mg) or excessive tissue quantity (e.g., 300mg) have on the dissociation effect? How to adjust the operation?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: Insufficient tissue quantity (50mg) will lead to a relative excess of enzyme reagents, which may cause over-digestion of cells and reduce cell viability. Excessive tissue quantity (300mg) will result in insufficient enzyme reagents, which cannot fully break down the extracellular matrix, leading to incomplete tissue dissociation, a large number of tissue clumps, and low single-cell yield. Adjustment methods: When the tissue quantity is insufficient, the dosage of each reagent can be reduced proportionally (e.g., Buffer A reduced to 540μL, Enzyme B to 200μL, Enzyme C to 2.5μL) to ensure the ratio of enzyme to tissue is appropriate. When the tissue quantity is excessive, it should be divided into two portions for processing, and each portion should be operated with the reagent dosage corresponding to 200mg of tissue to avoid insufficient reagents affecting dissociation.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e4. Q: In Step 3, the digestion time is 20-30 minutes, and quality inspection is required every 3-5 minutes. What is the specific operation of quality inspection? How to judge whether digestion needs to be stopped?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: Specific operation of quality inspection: Take 10μL of cell suspension, add 10μL of AOPI or trypan blue staining solution (self-prepared), mix well, drop it onto a cell counting plate, and observe under a cell counter or microscope. There are two criteria for judging whether to stop digestion: First, cell viability. If the viability is low, digestion must be stopped even if the tissue is not fully dissociated to avoid over-digestion. Second, cell dispersion. When the proportion of tissue clumps in the field of view is ≤5% and the proportion of single cells is ≥90%, digestion can be stopped. If there are still many tissue clumps after 30 minutes but the cell viability is ≥70%, digestion can be extended for 5 minutes, but quality inspection must be conducted again to avoid exceeding 35 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e5. Q: Both Step 5 and Step 6 require filtration with a 70μm cell sieve. Why does Step 6 require rinsing the original centrifuge tube and filtering again? What consequences will occur if the rinsing and filtering in Step 6 are omitted?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: The purpose of rinsing and filtering in Step 6 is to recover the cells remaining on the wall of the original centrifuge tube. Digested cells may adhere to the inner wall of the centrifuge tube, and filtering only in Step 5 will cause the loss of these cells, reducing the single-cell yield. If this step is omitted, the cell yield may decrease by 15%-20%. Especially for samples with a small initial tissue quantity, it will seriously affect subsequent experiments (e.g., single-cell sequencing requires a sufficient number of cells). Therefore, the tube wall must be rinsed with 3mL of RPMI 1640 medium or PBS containing 5% FBS, and the rinsing solution must be filtered through the same 70μm cell sieve to ensure full cell recovery.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e  6. Q: After adding DRS in Step 11, it is necessary to gently pipette 10 times with a 5mL pipette, and \"vortex oscillation is not allowed\". What problems will vortex oscillation cause? What impacts will insufficient or excessive pipetting times have?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: Vortex oscillation will generate strong mechanical force, which damages the cell membrane of mouse brain cells, leading to cell rupture. At the same time, it will cause uneven mixing of DRS and cell suspension, affecting the subsequent density gradient stratification effect. Insufficient pipetting times (less than 10 times) will result in insufficient mixing of DRS and cell suspension, making it impossible to form stable debris layers and cell layers after centrifugation, leading to incomplete debris removal. Excessive pipetting times (more than 15 times) will damage cells due to mechanical friction, increase the proportion of dead cells, and may disrupt the density system of DRS, also affecting stratification. It is necessary to strictly control the pipetting to 10 times, with gentle movements to avoid generating air bubbles.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e 7. Q: Step 12 requires slowly adding 3mL of PBS along the tube wall to \"gently cover the top layer, and never mix\". If mixing occurs accidentally during addition, how to handle it? Is it necessary to re-operate?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: If mixing occurs accidentally when adding PBS, it will destroy the density gradient foundation formed by DRS and cell suspension. After centrifugation, it will be impossible to clearly separate the debris layer, supernatant, and cell pellet, resulting in the failure of debris removal. Therefore, re-operation is mandatory. For re-operation, centrifuge the mixed solution at 300×g for 5 minutes at 4°C, discard the supernatant, resuspend the pellet with 2mL of PBS containing 5% FBS, then add 1mL of DRS and gently pipette 10 times according to Step 11. After that, slowly add 3mL of PBS along the tube wall again, ensuring no mixing to avoid re-occurrence of errors.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e 8. Q: Steps 13 and 14 emphasize \"a horizontal centrifuge must be used\" and \"the centrifuge tube must be handled gently\". What impact will using a vertical centrifuge or handling the tube roughly after centrifugation have on stratification?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: When a vertical centrifuge is used, the direction of centrifugal force is perpendicular to the centrifuge tube, making it impossible for the solution to be evenly stratified according to the density gradient. This will cause debris, cells, and PBS to mix, and no clear three-layer structure can be separated. Rough handling of the centrifuge tube after centrifugation will cause violent shaking of the solution inside the tube, and the formed three-layer structure (supernatant, debris layer, cell pellet) will mix with each other. Debris will re-mix into the cell pellet, resulting in the loss of debris removal effect. Therefore, a horizontal centrifuge must be used, and after centrifugation, the centrifuge tube should be slowly taken out with both hands supporting the bottom to avoid any violent shaking.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e 9. Q: When adding \u003ca href=\"red%20blood%20cell%20lysis%20buffer%20(FG-BA3311)\"\u003ered blood cell lysis buffer (FG-BA3311)\u003c\/a\u003e in Step 15, what volume is the \"three times the volume\" calculated based on? Can the red blood cell lysis time on ice (5 minutes) be shortened or extended?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: \"Three times the volume\" is calculated based on the \"appropriate volume of PBS containing 5% FBS\" in Step 15. For example, if 100μL of PBS containing 5% FBS is used to resuspend the pellet, 300μL of red blood cell lysis buffer should be added. The red blood cell lysis time on ice cannot be shortened or extended: Shortening it to less than 5 minutes will result in incomplete red blood cell lysis, and residual red blood cells will mix into the brain cell suspension, interfering with subsequent cell counting and experiments (e.g., non-specific signals of red blood cells in flow cytometry analysis). Extending it to more than 5 minutes will make the lysis buffer toxic to brain cells, leading to decreased brain cell viability, especially for sensitive neuron cells. Thus, the time must be strictly controlled at 5 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e 10. Q: After quality control in Step 21, it is required to \"carry out subsequent experiments immediately\". If subsequent experiments cannot be conducted immediately, can the prepared brain cell suspension be stored for a short period? What are the restrictions on storage conditions and time?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: Short-term storage is possible, but there are strict restrictions on storage conditions and time: The cell suspension needs to be adjusted to a concentration of 1×10⁶-1×10⁷ cells\/mL (using PBS containing 5% FBS), placed in a sterile low-adhesion centrifuge tube, sealed, and stored in a 4°C refrigerator. The storage time should not exceed 1 hour, and repeated shaking should be avoided during storage. If stored for more than 1 hour, the viability of brain cells will decrease significantly (may be lower than 60%), and a small amount of new cell debris will appear. If stored for more than 2 hours, the cells will basically lose their viability and cannot be used for subsequent experiments (e.g., cell culture, single-cell sequencing). Before use, trypan blue staining must be re-conducted for quality inspection, and only cells with viability ≥70% can be used.\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"2 reactions\/kit","offer_id":47833138856148,"sku":"FG-BA3305-2rxns","price":69.0,"currency_code":"USD","in_stock":true},{"title":"10 reactions\/kit","offer_id":46299523645652,"sku":"FG-BA3305-10rxns","price":289.0,"currency_code":"USD","in_stock":true},{"title":"50 reactions\/kit","offer_id":47682162524372,"sku":"FG-BA3305-50rxns","price":1329.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/files\/BA3305.png?v=1773991058"},{"product_id":"skin-dissociation-kit-fg-ba3307","title":"FireGene Skin Dissociation Kit for High-Viability Single-Cell Prep","description":"\u003ch3 data-start=\"264\" data-end=\"278\" class=\"\"\u003eOverview\u003c\/h3\u003e\n\u003cp data-start=\"279\" data-end=\"638\" class=\"\"\u003e\u003cstrong data-start=\"283\" data-end=\"317\"\u003eFireGene Skin Dissociation Kit\u003c\/strong\u003e is specifically designed for the efficient enzymatic dissociation of skin tissue into high-viability single-cell suspensions. Ideal for single-cell RNA sequencing, flow cytometry, and regenerative studies, this kit is optimized for sensitive skin-derived cells such as keratinocytes, fibroblasts, and immune populations.\u003c\/p\u003e\n\u003chr data-start=\"640\" data-end=\"643\" class=\"\"\u003e\n\u003ch3 data-start=\"645\" data-end=\"671\" class=\"\"\u003eBackground Information\u003c\/h3\u003e\n\u003cul data-start=\"673\" data-end=\"1893\"\u003e\n\u003cli data-start=\"673\" data-end=\"1380\" class=\"\"\u003e\n\u003cp data-start=\"675\" data-end=\"728\" class=\"\"\u003e\u003cstrong data-start=\"675\" data-end=\"728\"\u003eDriven by Clinical and Scientific Research Needs:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul data-start=\"731\" data-end=\"1380\"\u003e\n\u003cli data-start=\"731\" data-end=\"838\" class=\"\"\u003e\n\u003cp data-start=\"733\" data-end=\"838\" class=\"\"\u003eSkin single-cell sequencing is essential to dissect complex cell populations and intercellular signaling.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"841\" data-end=\"946\" class=\"\"\u003e\n\u003cp data-start=\"843\" data-end=\"946\" class=\"\"\u003eTraditional mechanical or chemical methods often fail to preserve \u003cstrong data-start=\"909\" data-end=\"945\"\u003ecellular diversity and viability\u003c\/strong\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"949\" data-end=\"1380\" class=\"\"\u003e\n\u003cp data-start=\"951\" data-end=\"968\" class=\"\"\u003eThis kit enables:\u003c\/p\u003e\n\u003cul data-start=\"973\" data-end=\"1380\"\u003e\n\u003cli data-start=\"973\" data-end=\"1067\" class=\"\"\u003e\n\u003cp data-start=\"975\" data-end=\"1067\" class=\"\"\u003eIdentification of \u003cstrong data-start=\"993\" data-end=\"1066\"\u003ekeratinocytes, fibroblasts, Langerhans cells, and dermal immune cells\u003c\/strong\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1072\" data-end=\"1167\" class=\"\"\u003e\n\u003cp data-start=\"1074\" data-end=\"1167\" class=\"\"\u003eIn-depth research into \u003cstrong data-start=\"1097\" data-end=\"1115\"\u003eskin disorders\u003c\/strong\u003e like psoriasis, atopic dermatitis, and skin cancer.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1172\" data-end=\"1254\" class=\"\"\u003e\n\u003cp data-start=\"1174\" data-end=\"1254\" class=\"\"\u003eDiscovery of \u003cstrong data-start=\"1187\" data-end=\"1225\"\u003ebiomarkers and therapeutic targets\u003c\/strong\u003e for dermatological diseases.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1259\" data-end=\"1380\" class=\"\"\u003e\n\u003cp data-start=\"1261\" data-end=\"1380\" class=\"\"\u003eAnalysis of gene expression profiles associated with \u003cstrong data-start=\"1314\" data-end=\"1343\"\u003eskin aging, wound healing\u003c\/strong\u003e, and \u003cstrong data-start=\"1349\" data-end=\"1379\"\u003ehair follicle regeneration\u003c\/strong\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1382\" data-end=\"1893\" class=\"\"\u003e\n\u003cp data-start=\"1384\" data-end=\"1428\" class=\"\"\u003e\u003cstrong data-start=\"1384\" data-end=\"1428\"\u003eBackground of Technological Development:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul data-start=\"1431\" data-end=\"1893\"\u003e\n\u003cli data-start=\"1431\" data-end=\"1536\" class=\"\"\u003e\n\u003cp data-start=\"1433\" data-end=\"1536\" class=\"\"\u003eDeveloped to address the inefficiencies and cell-damaging effects of conventional dissociation methods.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1539\" data-end=\"1781\" class=\"\"\u003e\n\u003cp data-start=\"1541\" data-end=\"1552\" class=\"\"\u003eIntegrates:\u003c\/p\u003e\n\u003cul data-start=\"1557\" data-end=\"1781\"\u003e\n\u003cli data-start=\"1557\" data-end=\"1632\" class=\"\"\u003e\n\u003cp data-start=\"1559\" data-end=\"1632\" class=\"\"\u003e\u003cstrong data-start=\"1559\" data-end=\"1588\"\u003eAdvanced enzyme cocktails\u003c\/strong\u003e tailored for complex skin matrix digestion.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1637\" data-end=\"1696\" class=\"\"\u003e\n\u003cp data-start=\"1639\" data-end=\"1696\" class=\"\"\u003ePrecisely calibrated \u003cstrong data-start=\"1660\" data-end=\"1695\"\u003ereaction times and temperatures\u003c\/strong\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1701\" data-end=\"1781\" class=\"\"\u003e\n\u003cp data-start=\"1703\" data-end=\"1781\" class=\"\"\u003eCarefully balanced enzymatic ratios based on \u003cstrong data-start=\"1748\" data-end=\"1780\"\u003eextracellular matrix biology\u003c\/strong\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1784\" data-end=\"1893\" class=\"\"\u003e\n\u003cp data-start=\"1786\" data-end=\"1893\" class=\"\"\u003eResults in higher \u003cstrong data-start=\"1804\" data-end=\"1827\"\u003ecell recovery rates\u003c\/strong\u003e, improved \u003cstrong data-start=\"1838\" data-end=\"1856\"\u003ecell viability\u003c\/strong\u003e, and \u003cstrong data-start=\"1862\" data-end=\"1892\"\u003ebatch-to-batch consistency\u003c\/strong\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"1895\" data-end=\"1898\" class=\"\"\u003e\n\u003ch3 data-start=\"1900\" data-end=\"1923\" class=\"\"\u003eDetection Principle\u003c\/h3\u003e\n\u003cul data-start=\"1925\" data-end=\"2481\"\u003e\n\u003cli data-start=\"1925\" data-end=\"2220\" class=\"\"\u003e\n\u003cp data-start=\"1927\" data-end=\"1982\" class=\"\"\u003eEmploys a \u003cstrong data-start=\"1937\" data-end=\"1981\"\u003esynergistic enzymatic digestion protocol\u003c\/strong\u003e:\u003c\/p\u003e\n\u003cul data-start=\"1985\" data-end=\"2220\"\u003e\n\u003cli data-start=\"1985\" data-end=\"2055\" class=\"\"\u003e\n\u003cp data-start=\"1987\" data-end=\"2055\" class=\"\"\u003eSkin samples are \u003cstrong data-start=\"2004\" data-end=\"2021\"\u003efinely minced\u003c\/strong\u003e for better enzymatic penetration.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2058\" data-end=\"2137\" class=\"\"\u003e\n\u003cp data-start=\"2060\" data-end=\"2137\" class=\"\"\u003eA sequence of \u003cstrong data-start=\"2074\" data-end=\"2094\"\u003emultiple enzymes\u003c\/strong\u003e is added for progressive matrix breakdown.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2140\" data-end=\"2220\" class=\"\"\u003e\n\u003cp data-start=\"2142\" data-end=\"2220\" class=\"\"\u003eReactions are conducted under \u003cstrong data-start=\"2172\" data-end=\"2219\"\u003eoptimized temperature and timing conditions\u003c\/strong\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2221\" data-end=\"2481\" class=\"\"\u003e\n\u003cp data-start=\"2223\" data-end=\"2239\" class=\"\"\u003eProcess Outcome:\u003c\/p\u003e\n\u003cul data-start=\"2242\" data-end=\"2481\"\u003e\n\u003cli data-start=\"2242\" data-end=\"2340\" class=\"\"\u003e\n\u003cp data-start=\"2244\" data-end=\"2340\" class=\"\"\u003eEnzymes gradually \u003cstrong data-start=\"2262\" data-end=\"2305\"\u003edisrupt extracellular matrix components\u003c\/strong\u003e and \u003cstrong data-start=\"2310\" data-end=\"2339\"\u003eintercellular connections\u003c\/strong\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2343\" data-end=\"2481\" class=\"\"\u003e\n\u003cp data-start=\"2345\" data-end=\"2481\" class=\"\"\u003eYields a \u003cstrong data-start=\"2354\" data-end=\"2393\"\u003ehigh-quality single-cell suspension\u003c\/strong\u003e, ideal for downstream analysis such as scRNA-seq, immunophenotyping, or culture assays.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 data-start=\"1900\" data-end=\"1923\" class=\"\"\u003eSpecifications\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eSingle-cell sequencing, cell culture or other cell-related detections\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eCompatible Sample Types\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eSkin tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eSupported Instruments\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eWater bath, horizontal centrifuge, cell counter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e-20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eShelf-life\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 data-start=\"1900\" data-end=\"1923\" class=\"\"\u003eKit Components\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e10 reactions\/kit\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"table-wrapper\"\u003e\n\u003ctable height=\"150\" style=\"width: 100.061%;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"width: 61.7723%;\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"width: 37.9491%;\"\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 61.7723%;\"\u003e\u003cspan\u003eSDS1 (Skin Dissociation Solution 1)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.9491%;\"\u003e\u003cspan\u003e2 × 1.1 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 61.7723%;\"\u003e\u003cspan\u003eSDS1 (Skin Dissociation Solution 2)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.9491%;\"\u003e\u003cspan\u003e2 × 1.4 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e50 reactions\/kit\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"table-wrapper\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"table-wrapper\"\u003e\n\u003ctable height=\"150\" style=\"width: 100.061%;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"width: 61.7723%;\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"width: 37.9491%;\"\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 61.7723%;\"\u003e\u003cspan\u003eSDS1 (Skin Dissociation Solution 1)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.9491%;\"\u003e\u003cspan\u003e10 × 1.1 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 61.7723%;\"\u003e\u003cspan\u003eSDS1 (Skin Dissociation Solution 2)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 37.9491%;\"\u003e\u003cspan\u003e10 × 1.4 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4\u003eProduct Q\u0026amp;A\u003c\/h4\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e1. Q: Is this kit suitable for skin tissue of all mammals? Are there any requirements for the state of skin tissue (e.g., fresh, frozen)? Can it be used for skin tumor tissue?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: The kit is suitable for normal skin tissue of common mammals such as humans and mice, but it is not recommended for direct use with skin tumor tissue. The cell density and extracellular matrix composition of tumor tissue are significantly different from those of normal skin, so it is necessary to contact the manufacturer to confirm whether enzyme digestion parameters need to be adjusted. Meanwhile, the kit only supports the dissociation of fresh skin tissue. Frozen tissue will have damaged cell structures due to ice crystal formation, resulting in extremely low cell viability after dissociation. If frozen tissue must be used, it should first be processed through a dedicated freeze-thaw recovery protocol before attempting dissociation.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e2. Q: The kit is labeled \"10 reactions\". What are the respective dosages of Skin Tissue Dissociation Solution 1 and Dissociation Solution 2 per experiment? If only 50mg of tissue needs to be processed in a single experiment, can the reagent dosage be reduced proportionally?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: For each experiment (200mg of tissue), 210μL of Skin Tissue Dissociation Solution 1 and 280μL of Skin Tissue Dissociation Solution 2 are required. If processing 50mg of tissue (1\/4 of the standard dosage), the reagent dosage can be reduced proportionally (52.5μL of Dissociation Solution 1 + 697.5μL of RPMI 1640 medium, 70μL of Dissociation Solution 2 + 680μL of RPMI 1640 medium). However, note that when the reagent volume is too small, pipetting errors will increase. It is recommended to adjust the volume to at least 100μL (e.g., halve the dosage for 100mg of tissue). If 50mg of tissue must be processed, a high-precision pipette should be used to ensure the accuracy of the reagent ratio.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e3. Q: In Step 6, mouse skin requires 0.5 hours of digestion, human skin requires overnight digestion, and the digestion time for other mammals ranges from \"1 to several hours\". How to specifically determine the digestion endpoint? What are the impacts of insufficient or excessive digestion time?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: The digestion endpoint must be determined through \"regular quality inspection\": take 10μL of the suspension every 30 minutes, stain with trypan blue, and observe under a microscope. Digestion can be stopped if the cell viability is ≥70% and the proportion of tissue clumps is ≤10%. Insufficient digestion will result in incomplete tissue dissociation, low single-cell yield, and a large amount of residual tissue debris during subsequent filtration. Excessive digestion will cause over-enzyme hydrolysis of cells, leading to damaged cell membranes and viability dropping below 60%, making the cells unsuitable for single-cell sequencing or cell culture. For example, when processing rat skin, it is recommended to first digest for 0.5 hours, then adjust based on quality inspection results (extend by 15 minutes if viability is high, stop immediately if viability is low).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e4. Q: Both Step 3 and Step 6 mention \"37°C water bath or hybridization oven\". Is the only operational difference between the two devices \"manual shaking\" and \"automatic rotation speed\"? Which device is better for dissociation results?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: The core differences include not only the mixing method but also temperature stability: the temperature control accuracy of the hybridization oven (±0.5°C) is higher than that of the water bath (±1°C). Additionally, the automatic rotation speed (20-30 rpm) ensures uniform contact between tissue and dissociation solution, avoiding uneven local digestion. The water bath requires manual shaking; inconsistent force or frequency may lead to over-digestion of some tissues and under-digestion of others. For selection: if processing a large number of samples (≥5) or pursuing experimental reproducibility, the hybridization oven is better. If processing a small number of samples (1-2) and no hybridization oven is available, manual operation with a water bath is acceptable, but it is necessary to shake once every 4 minutes for 10 seconds each time.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e5. Q: Steps 8-10 require filtration with a 70μm cell sieve and rinsing the centrifuge tube 3 times, collecting a total of 12mL of filtrate. What impact will omitting one rinsing step have on the experimental results? Can a 40μm cell sieve replace the 70μm one?\u003c\/strong\u003e\u003c\/span\u003e\u003cspan\u003eA: Omitting one rinsing step will result in the loss of approximately 1\/3 of residual cells, reducing the final cell yield by 20%-30%. Especially after overnight digestion of human skin, cells are prone to adhering to the tube wall, so 3 rinsing steps are mandatory. A 40μm cell sieve cannot replace the 70μm one: the diameter of single cells after skin dissociation is approximately 10-15μm, and the 70μm sieve can filter tissue debris without retaining cells. The 40μm sieve has a too-small pore size, which is easily clogged by cell clumps or fibrous impurities, leading to filtration difficulties and potential cell damage due to extrusion, reducing viability.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e6. Q: Steps 12-13 require washing the pellet twice with PBS containing 5% FBS. What is the function of FBS? Can it be replaced with PBS without FBS or serum substitutes?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: The core function of FBS is to neutralize the activity of residual dissociation solution, preventing enzymes from continuing to damage cells, and to provide nutrients for cells to maintain viability. It cannot be replaced with PBS without FBS; otherwise, residual enzymes will continue to damage cell membranes, reducing cell viability by more than 30% after washing. Serum substitutes (e.g., BSA) can only provide nutrients but cannot neutralize enzyme activity, so they are not recommended as substitutes. If serum-free experiments are required (e.g., certain cell sorting scenarios), an \"enzyme inhibitor\" (contact the manufacturer for a compatible model) must be added after washing; otherwise, cell viability cannot be guaranteed.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e7. Q: The instruction manual states that \"DMEM medium can replace RPMI 1640 medium\". After replacement, is it necessary to adjust the reagent dosage or digestion time? Does the choice of the two media affect cell viability?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: No adjustment to reagent dosage or digestion time is needed after replacement. Both DMEM and RPMI 1640 are common basal media for mammalian cells. Although they differ in amino acid and vitamin content, both can provide a suitable osmotic pressure and pH environment for skin tissue dissociation, with no impact on dissociation efficiency. The impact on cell viability is minimal: experimental data shows that the difference in cell viability after dissociating mouse skin with the two media is ≤5%. The medium can be selected based on the existing inventory in the laboratory, with no need for deliberate replacement.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e8. Q: If there are many red blood cells in the cell suspension after dissociation and \u003ca href=\"https:\/\/firegene.com\/products\/red-blood-cell-lysis-kit-fg-ba3311?_pos=3\u0026amp;_sid=a16db81c5\u0026amp;_ss=r\"\u003eFG-BA3311 Red Blood Cell Lysis Buffer\u003c\/a\u003e is required for removal, at which step should this operation be performed? What precautions should be taken during lysis?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eA: The operation should be performed after Step 13 and before Step 14: after completing the two washing steps in Step 13 and discarding the supernatant, add 1mL of \u003ca href=\"https:\/\/firegene.com\/products\/red-blood-cell-lysis-kit-fg-ba3311?_pos=1\u0026amp;_sid=fa234d8ea\u0026amp;_ss=r\"\u003eFG-BA3311 Red Blood Cell Lysis Buffer\u003c\/a\u003e, incubate at room temperature for 5 minutes, centrifuge at 300×g for 5 minutes at 4°C, discard the supernatant, and resuspend the pellet with 5mL of PBS containing 5% FBS (equivalent to an additional washing step) before proceeding to Step 14. Precautions: The lysis time should not exceed 8 minutes, otherwise skin cells will be damaged. If there are excessive red blood cells (e.g., skin samples containing a large number of capillaries), lysis can be repeated once, but an additional washing step is required to avoid residual lysis buffer.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e9. Q: The kit needs to be stored at -20°C. If the ice pack melts during transportation and the reagent is left at 4°C for 2 hours, can it still be used? What are the impacts of repeated freezing and thawing?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: The reagent can still be used after being left at 4°C for 2 hours, but it should be immediately returned to -20°C, and fully mixed before subsequent use. Repeated freezing and thawing will reduce the enzyme activity in the dissociation solution: each freeze-thaw cycle reduces enzyme activity by 10%-15%; after more than 3 cycles, the activity will be less than 50%, making it unable to effectively dissociate tissue. It is recommended that after receiving the kit, immediately aliquot Dissociation Solution 1 and Dissociation Solution 2 into single-use volumes (e.g., 210μL\/tube for Dissociation Solution 1, 280μL\/tube for Dissociation Solution 2), seal them, store at -20°C, and take one tube per experiment to avoid repeated freezing and thawing.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e10. Q: After quality control in Step 15, it is required to \"carry out subsequent experiments immediately\". If subsequent experiments cannot be conducted immediately, can the prepared cell suspension be stored for a short period? What are the restrictions on storage conditions and time?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: Short-term storage is possible under the conditions of sealed storage in a 4°C refrigerator for no more than 2 hours, with repeated shaking avoided. During storage, the cell concentration should be adjusted to 1×10⁶-1×10⁷ cells\/mL using PBS containing 5% FBS, and the suspension should be placed in a low-adhesion centrifuge tube. After 2 hours, cell viability will decrease significantly (10%-15% per hour), and new cell debris will be generated. If stored for more than 4 hours, the cells will basically lose viability and cannot be used for downstream experiments. Before use, re-quality inspection is required, and only cells with viability ≥65% can be used.\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"2 reactions\/kit","offer_id":47833315410132,"sku":"FG-BA3307-2rxns","price":69.0,"currency_code":"USD","in_stock":true},{"title":"10 reactions\/kit","offer_id":46299523678420,"sku":"FG-BA3307-10rxns","price":289.0,"currency_code":"USD","in_stock":true},{"title":"50 reactions\/kit","offer_id":47679444189396,"sku":"FG-BA3307-50rxns","price":739.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/files\/BA3307.png?v=1773935344"},{"product_id":"blood-vessel-dissociation-kit-fg-ba3310","title":"FireGene Blood Vessel Dissociation Kit for Single-Cell Applications","description":"\u003ch3 id=\"overview\"\u003eOverview\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Blood Vessel Dissociation Kit\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eis specifically developed for the efficient enzymatic dissociation of vascular tissues into high-viability single-cell suspensions. This advanced kit is ideal for applications in single-cell sequencing, vascular biology, and drug discovery.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch3 id=\"background-information\"\u003eBackground Information\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eDriven by Clinical and Scientific Research Needs:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eUnderstanding cellular heterogeneity in vascular tissues is vital for investigating\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003evascular diseases\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003esuch as\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eatherosclerosis\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eand\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003evasculitis\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eConventional methods often damage delicate vascular cells or fail to isolate distinct populations.\u003c\/li\u003e\n\u003cli\u003eThis kit enables:\n\u003cul\u003e\n\u003cli\u003eIdentification of\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eendothelial cells, smooth muscle cells, fibroblasts, and immune infiltrates\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eDiscovery of\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ebiomarkers and gene targets\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eassociated with\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003evascular inflammation and remodeling\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eAdvancement of clinical research and\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003etherapeutic development\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003ein cardiovascular pathology.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBackground of Technological Development:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDesigned to improve upon the\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003elow efficiency and high cell damage\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003erates of traditional techniques.\u003c\/li\u003e\n\u003cli\u003eIntegrates:\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eCarefully selected enzymatic blends\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003efor vascular matrix degradation.\u003c\/li\u003e\n\u003cli\u003eOptimized parameters for\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eenzyme concentration, temperature, and incubation duration\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eWorkflow that ensures\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eenhanced cell viability\u003c\/strong\u003e,\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ehigher recovery rates\u003c\/strong\u003e, and\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003econsistent performance\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eacross experiments.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch3 id=\"detection-principle\"\u003eDetection Principle\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eUtilizes a\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003emulti-enzyme digestion protocol\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003esuited to the unique composition of blood vessel tissue.\u003c\/li\u003e\n\u003cli\u003eProcess:\n\u003cul\u003e\n\u003cli\u003eTissue is\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003efinely sectioned\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eto facilitate penetration.\u003c\/li\u003e\n\u003cli\u003eEnzymes are applied\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003esequentially under controlled temperature\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eand timing.\u003c\/li\u003e\n\u003cli\u003eMatrix components and cellular junctions are\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eenzymatically degraded\u003c\/strong\u003e.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003eResults in:\n\u003cul\u003e\n\u003cli\u003eA\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eclean, high-quality single-cell suspension\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eready for scRNA-seq, FACS, or downstream analysis.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 id=\"detection-principle\"\u003eSpecifications\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eSingle-cell sequencing, cell culture or other cell-related detections\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eCompatible Sample Types\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eBlood vessel tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eSupported Instruments\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eWater bath, horizontal centrifuge, cell counter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e-20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eShelf-life\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 id=\"detection-principle\"\u003eKit Components\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e10 Reactions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 60%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003e10 Reactions\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e\u003cspan\u003eBVDS1 (Blood vessel dissociation solution 1) \u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e2 × 1.1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e\u003cspan\u003eBVDS1 (Blood vessel dissociation solution 2) \u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e4 × 1.35 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e50 Reactions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 60%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003e50 Reactions\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e\u003cspan\u003eBVDS1 (Blood vessel dissociation solution 1) \u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e10 × 1.1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e\u003cspan\u003eBVDS1 (Blood vessel dissociation solution 2) \u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e\n\u003cp\u003e20 × 1.35 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cdiv class=\"type-page\"\u003e\n\u003cdiv class=\"product-content\"\u003e\n\u003cdiv class=\"con-wrap\"\u003e\n\u003cdiv class=\"jianjie tab selected\"\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e1.    Q: Is this kit suitable for vascular tissue of all mammals? Are there differences in dissociation effects on arteries, veins, and capillaries? Can it be used for vascular tumor tissue (e.g., angiosarcoma)?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The kit is suitable for normal vascular tissue (including arteries and veins) of mammals such as humans and mice, but it is not recommended for capillaries or vascular tumor tissue for the time being. Capillaries have extremely thin walls (only 1-2 cell layers), which are prone to rupture during dissociation, making it difficult to obtain intact single cells. The cell structure and extracellular matrix of vascular tumor tissue are significantly different from those of normal blood vessels, and the enzymatic hydrolysis parameters do not match, which may lead to incomplete dissociation or low cell viability. There are slight differences in the dissociation effects on arteries and veins: due to the thicker wall and rich elastic fibers of arteries, the incubation time of Dissociation Solution 2 needs to be 20-30 minutes longer than that of veins, and the specific duration should be adjusted through quality inspection.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e2.    Q: The kit is labeled \"10 rxns\". What are the respective dosages of Vascular Tissue Dissociation Solution 1 and Dissociation Solution 2 per experiment? If only 100mg of vascular tissue is processed in a single experiment, can the reagent dosage be reduced proportionally?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: For each experiment (200mg of tissue), 210μL of Vascular Tissue Dissociation Solution 1 and 530μL of Vascular Tissue Dissociation Solution 2 are required. If 100mg of tissue (1\/2 of the standard dosage) is processed, the reagent dosage can be reduced proportionally: Dissociation Solution 1 is reduced to 105μL (matched with 1395μL of RPMI 1640 medium), and Dissociation Solution 2 is reduced to 265μL (matched with 1235μL of RPMI 1640 medium). It should be noted that the reagent volume must ensure pipetting accuracy (single pipetting volume is not less than 50μL). If the tissue volume is less than 50mg, it is not recommended to further reduce the dosage, so as to avoid pipetting errors caused by too small volume, which will destroy the proportional balance between enzymes and tissues and affect the dissociation effect.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e3.    Q: Both Step 3 and the incubation of Dissociation Solution 2 require a \"37°C water bath or hybridization oven\". Besides \"manual shaking\" and \"automatic rotation speed\", what other precautions are there for the operation of the two devices? Which one is more suitable for vascular tissue dissociation?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Additional precautions: ① The water bath needs to check the water level regularly (ensure that the centrifuge tube is immersed more than 1\/2 of the water surface to avoid uneven local temperature); ② The hybridization oven needs to be preheated 10 minutes in advance to ensure that the temperature inside the cavity is stable before putting in the sample. In terms of effect, the hybridization oven is more suitable for vascular tissue dissociation: vascular tissue (especially arteries) has a relatively tough texture, and manual shaking is prone to \"local excessive enzymatic hydrolysis and local undigestion\"; the automatic rotation speed of 20-30 rpm of the hybridization oven can make the dissociation solution evenly wrap the tissue, avoid the accumulation of fiber components, and the single-cell yield is 15%-20% higher than that of the water bath. If a water bath is used, it is necessary to shake it strictly once every 3 minutes for 10 seconds each time to ensure that no tissue sinks to the bottom.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e4.    Q: Step 7 mentions \"quality inspection at regular intervals\". What are the quality inspection intervals and judgment criteria for vascular tissue dissociation? If low cell viability is found during quality inspection but there are still undigested vascular debris, how to deal with it?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Quality inspection interval: In the first 1 hour of incubation with Dissociation Solution 2, quality inspection is conducted once every 20 minutes; after 1 hour, quality inspection is conducted once every 30 minutes. Quality inspection operation: Take 10μL of suspension, stain with trypan blue, and observe under a microscope to check the number and viability. The elastic fibers and collagen fibers in vascular tissue will adsorb enzymes, and continuous incubation will cause the enzymes to act excessively on cells rather than debris; the residual debris can be removed by filtration through a 70μm cell sieve, and complete dissociation is not required to avoid further decline in cell viability.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e5.    Q: Steps 8-10 require filtration with a 70μm cell sieve and rinsing the centrifuge tube 3 times to collect a total of 12mL of filtrate. What impact will omitting one rinsing step have on the experimental results? Can a 40μm cell sieve replace the 70μm one?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Omitting one rinsing step will result in the loss of about 1\/3 of the residual cells, reducing the final cell yield by 25%-30%. Cells after vascular dissociation (such as endothelial cells and smooth muscle cells) are easily adsorbed on the inner wall of the centrifuge tube, and 3 rinsing steps are the key to ensuring full cell recovery. A 40μm cell sieve cannot replace the 70μm one: the pore size of the 40μm sieve is too small, which will retain some single cells derived from blood vessels (for example, the diameter of endothelial cells is about 8-12μm, which can pass through, but the sieve is easily blocked by fiber debris, leading to difficult filtration and even cell extrusion to reduce viability); the 70μm sieve can not only filter debris but also ensure that single cells pass through smoothly, which is the best choice.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e6.    Q: The instruction manual mentions that \"DMEM medium can replace RPMI 1640 medium\". After replacement, is it necessary to adjust the reagent dosage or incubation time? Is there any difference in the impact of the two media on the viability of vascular cells?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: There is no need to adjust the reagent dosage or incubation time after replacement. Both DMEM and RPMI 1640 are common basic media for mammalian cells. Although there are differences in glucose and amino acid content, both can provide a suitable osmotic pressure (280-320mOsm\/kg) and pH (7.2-7.4) for vascular tissue dissociation, and have no impact on the enzymatic hydrolysis efficiency. The impact on cell viability is minimal: you can choose freely according to the laboratory inventory without deliberate replacement.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e7.    Q: If there are many red blood cells in the cell suspension after dissociation and BA3311 Red Blood Cell Lysis Buffer is needed to remove them, at which step should this operation be performed? What should be noted during lysis to avoid damaging vascular cells?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The operation should be performed after Step 13 and before Step 14: after completing two washing steps in Step 13 and discarding the supernatant, add 1mL of BA3311 Red Blood Cell Lysis Buffer, incubate in an ice bath for 5 minutes (instead of room temperature to reduce toxicity to vascular cells), discard the supernatant, then resuspend with 5mL of PBS containing 5% FBS (add an additional washing step), and then proceed to Step 14. Precautions: ① The lysis time should not exceed 8 minutes, and the ice bath can slow down the damage of the lysis buffer to vascular cells (especially endothelial cells); ② If there are too many red blood cells, lysis can be repeated once, but PBS washing is required again to avoid residual lysis buffer affecting subsequent experiments (such as cell capture efficiency in single-cell sequencing).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e8.    Q: The kit needs to be stored at -20°C. If the ice pack melts during transportation and the reagent is placed at 4°C for 2 hours, can it still be used? What impact does repeated freezing and thawing have on the dissociation solution?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: It can be used continuously if placed at 4°C for 2 hours, but it must be immediately returned to -20°C and fully mixed before subsequent use. The enzymes (such as collagenase and elastase) in the dissociation solution lose ≤10% of their activity when placed at 4°C for a short time (≤2 hours), which does not affect the dissociation effect. Repeated freezing and thawing will lead to a significant decrease in enzyme activity: each freeze-thaw cycle reduces enzyme activity by 12%-18%; after more than 3 freeze-thaw cycles, the activity is less than 50%, which cannot effectively decompose the fiber components of vascular tissue, resulting in a large number of tissue blocks after dissociation. It is recommended that after receiving the kit, aliquot Dissociation Solution 1 (2×1.1mL) into 110μL\/tube and Dissociation Solution 2 (4×1.35mL) into 270μL\/tube, seal them and store at -20°C. Take 2 tubes of Dissociation Solution 1 and 2 tubes of Dissociation Solution 2 (corresponding to 1 dose) for each experiment to avoid repeated freezing and thawing.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e9.    Q: In Step 6, the incubation time of Dissociation Solution 2 is \"30 minutes - 3 hours\". How to set the initial incubation time for different types of vascular tissue (such as mouse aorta and human vein)?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Recommendations for initial incubation time: ① Mouse aorta (thick-walled artery): incubate for 1 hour first, then adjust through quality inspection; ② Mouse vein (thin-walled vein): incubate for 30 minutes first, then adjust through quality inspection; ③ Human aorta (thicker, richer in fiber components): incubate for 1.5 hours first, then adjust through quality inspection; ④ Human vein: incubate for 45 minutes first, then adjust through quality inspection. Core principle: the thicker the vessel wall and the more fiber components, the longer the initial incubation time, but dynamic adjustment is required through quality inspection. If the viability is ≥75% and there is little debris after 1 hour, the incubation can be stopped in advance; if the viability is low, even if there is much debris, the incubation must be terminated to avoid excessive digestion.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e10.    Q: After quality control in Step 15, it is required to \"carry out subsequent experiments immediately\". If subsequent experiments cannot be carried out immediately, can the prepared vascular cell suspension be stored for a short time? What are the restrictions on storage conditions and time?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Short-term storage is possible. The storage condition is sealed storage in a 4°C refrigerator for no more than 1.5 hours, and repeated shaking should be avoided. During storage, the cell concentration should be adjusted to 1×10⁶-1×10⁷ cells\/mL with PBS containing 5% FBS, and placed in a low-adhesion centrifuge tube. After 1.5 hours, the viability of vascular cells (especially endothelial cells) will decrease significantly (decreasing by 10%-12% per hour), and cell aggregation is prone to occur; if stored for more than 3 hours, the cell viability may be lower than 50%, which cannot be used for single-cell sequencing or cell culture. Before use, re-quality inspection is required, and only cells with viability ≥65% can be used. In addition, gently pipette 5-8 times to disperse slightly aggregated cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cfooter\u003e\n\u003cdiv class=\"footer-head pt50 pb50 sm-dn\"\u003e\n\u003cdiv class=\"mauto dflr pt25 pb10\"\u003e\n\u003cdl\u003e\u003c\/dl\u003e\n\u003cdl\u003e\n\u003cdd\u003e\u003c\/dd\u003e\n\u003c\/dl\u003e\n\u003cdiv class=\"txt pl40 pr20 li25\"\u003e\n\u003cdiv class=\"footer-logo\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/footer\u003e","brand":"FireGene","offers":[{"title":"2 reactions\/kit","offer_id":47833332318420,"sku":"FG-BA3310-2rxns","price":249.0,"currency_code":"USD","in_stock":true},{"title":"10 reactions\/kit","offer_id":46299523711188,"sku":"FG-BA3310-10rxns","price":1009.0,"currency_code":"USD","in_stock":true},{"title":"50 reactions\/kit","offer_id":47705529057492,"sku":"FG-BA3310-50rxns","price":4009.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/files\/BA3310.png?v=1774234884"},{"product_id":"tumor-dissociation-kit-fg-ba3320","title":"FireGene Tumor Dissociation Kit - Optimized for Tumor Microenvironment","description":"\u003ch3 id=\"overview\"\u003eOverview\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Tumor Dissociation Kit\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eis optimized for the enzymatic dissociation of tumor tissue into high-viability single-cell suspensions. Tailored for downstream applications such as single-cell RNA sequencing and functional analysis, this kit supports researchers in profiling the complex tumor microenvironment.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch3 id=\"background-information\"\u003eBackground Information\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eDriven by Clinical and Scientific Research Needs:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSingle-cell sequencing enables detailed insights into\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003etumor heterogeneity and progression\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eTraditional dissociation methods fail to preserve fragile or rare cell types within tumor tissues.\u003c\/li\u003e\n\u003cli\u003eThe kit facilitates:\n\u003cul\u003e\n\u003cli\u003eIsolation of diverse tumor-associated cells such as\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003emalignant cells, stem-like cells, and immune cells\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eExploration of\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003etreatment resistance\u003c\/strong\u003e, tumor development, and\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eimmune infiltration\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eIdentification of\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ebiomarkers and spatial expression profiles\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003efor drug discovery and personalized medicine.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBackground of Technological Development:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eConventional mechanical and chemical techniques often compromise cell viability and reproducibility.\u003c\/li\u003e\n\u003cli\u003eThis kit uses:\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eRefined enzyme mixtures\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eselected based on tumor extracellular matrix characteristics.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eOptimized reaction parameters\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003efor precise, efficient dissociation.\u003c\/li\u003e\n\u003cli\u003eA process that preserves cell diversity and yields\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ehigh-quality single-cell suspensions\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003econsistently.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch3 id=\"detection-principle\"\u003eDetection Principle\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eImplements a\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003emulti-step enzymatic digestion method\u003c\/strong\u003e:\n\u003cul\u003e\n\u003cli\u003eTumor samples are\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eminced into small pieces\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTwo-stage enzymatic reagents\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eare added sequentially.\u003c\/li\u003e\n\u003cli\u003eDigestion proceeds under\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ecarefully controlled temperature and timing\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eEnzymes break down\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eECM components and intercellular adhesions\u003c\/strong\u003e.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003eFinal result:\n\u003cul\u003e\n\u003cli\u003eA\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eclean, viable single-cell suspension\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eready for downstream applications like scRNA-seq, cytometry, or organoid development.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 id=\"detection-principle\"\u003eSpecifications\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eSingle-cell sequencing, cell culture or other cell-related detections\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eCompatible Sample Types\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eTumor tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eSupported Instruments\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eWater bath, horizontal centrifuge, cell counter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e-20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eShelf-life\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 id=\"detection-principle\"\u003eKit Components\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e10 Reactions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 60%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003e10 Reactions\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e\u003cspan\u003eTumor DS (Tumor Dissociation Solution)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e2 × 1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e50 Reactions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 60%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003e50 Reactions\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e\u003cspan\u003eTumor DS (Tumor Dissociation Solution)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e10 × 1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"type-page\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"type-page\"\u003e\n\u003cdiv class=\"product-content\"\u003e\n\u003cdiv class=\"con-wrap\"\u003e\n\u003cdiv class=\"jianjie tab selected\"\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eProduct FAQ\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e1.    Q: Is this kit only suitable for fresh tumor tissue? Is it effective for cryopreserved and resuscitated tumor tissue? Can it be used for non-solid tumor samples (e.g., hematological tumor cells)?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The kit is mainly suitable for fresh solid tumor tissues (such as lung cancer, breast cancer, and liver cancer tissues). It should be used with caution for cryopreserved and resuscitated tumor tissues — the cryopreservation process may cause structural damage to tumor cells, and the cell viability after dissociation will be 20%-30% lower than that of fresh tissues. Trypan blue staining is required for pre-quality inspection (viability ≥60% to be usable). It is not suitable for non-solid tumor samples. Hematological tumors (such as leukemia and lymphoma) are mainly composed of suspended cells, which do not require tissue dissociation and can be processed directly with a cell sorting kit. The enzymolysis system of this kit is not compatible with suspended tumor cells and may cause massive cell death.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e2.    Q: The instruction manual requires cutting 200mg of fresh tumor tissue. Will insufficient tissue quantity (e.g., 80mg) or excessive tissue quantity (e.g., 300mg) affect the dissociation effect? How to adjust the operation?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Yes, it will affect the effect. ① Insufficient tissue quantity (\u0026lt;150mg): The dissociation solution is relatively excessive, and the high enzyme concentration easily leads to over-digestion of tumor cells, resulting in a viability decrease of more than 40%. It is recommended to combine multiple 80mg tissue samples (to a total weight of 180-200mg) and add the dissociation solution according to the conventional dosage to ensure the ratio of enzyme to tissue is appropriate. ② Excessive tissue quantity (\u0026gt;250mg): The space in the 5mL centrifuge tube is limited, so the tissue cannot be fully dispersed, and some areas may have incomplete enzymolysis, leaving a large amount of tissue debris. The tissue needs to be processed in two tubes, with each tube containing 180-220mg of tissue and corresponding to 240μL of dissociation solution + 2760μL of RPMI 1640 medium, to avoid the impact of tissue crowding on enzymolysis efficiency.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e3.    Q: The digestion time in Step 3 is 0.5-2 hours. How to determine the initial digestion time for different types of tumor tissues (e.g., lung cancer, breast cancer, liver cancer)? What consequences will occur if the digestion time is insufficient or excessive?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The initial digestion time needs to be adjusted according to the density of the tumor tissue: ① Tumors with low density (such as some lung cancers and solid foci of lymphoma): The initial digestion time is 0.5-1 hour. Such tissues have low fiber content and are easy to enzymolyze. ② Tumors with high density (such as breast cancer and liver cancer): The initial digestion time is 1-1.5 hours. Such tissues contain more collagen fibers and require a longer time for decomposition. Insufficient digestion time: The tissue is not fully dissociated, and a large number of clusters of more than 10 cells can be seen under the microscope, with a single-cell yield of less than 50%. Excessive digestion time: Tumor cells are excessively damaged, with viability reduced to less than 50%, and cell debris is prone to appear, which interferes with the preparation of subsequent single-cell sequencing samples. Dynamic adjustment of the termination time through quality inspection every 20 minutes is required.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e4.    Q: Steps 5 and 6 require filtration with a 70μm cell sieve and rinsing the centrifuge tube 3 times to collect a total of 12mL of filtrate. What impact will omitting one rinsing step or replacing with a 50μm\/100μm sieve have?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Omitting one rinsing step will result in the loss of approximately 1\/3 of residual tumor cells, reducing the final cell yield by 25%-30%. Tumor cells are easily adsorbed on the inner wall of the centrifuge tube, and 3 rinsing steps are the key to ensuring full cell recovery. A 50μm or 100μm sieve cannot be used as a replacement: The pore size of the 50μm sieve is too small and will retain some single tumor cells (e.g., small cell lung cancer cells have a diameter of about 8-12μm; although they can pass through, the sieve is easily blocked by fiber debris, leading to filtration difficulties and even cell extrusion to reduce viability). The pore size of the 100μm sieve is too large and cannot effectively filter undigested tissue fragments. These fragments will enter subsequent steps along with cells, which not only interferes with the accuracy of cell counting but also may block the single-cell sequencing chip.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e5.    Q: The instruction manual mentions that \"DMEM medium can replace RPMI 1640 medium\". After replacement, is it necessary to adjust centrifugation parameters or digestion time? Is there any difference in the impact of the two media on the viability of tumor cells?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: There is no need to adjust centrifugation parameters (still 4℃, 300×g for 5 minutes) or digestion time after replacement. Both DMEM and RPMI 1640 are common basal media for mammalian cells. Although they differ in glucose and amino acid content, both can provide a suitable osmotic pressure (280-320mOsm\/kg) and pH (7.2-7.4) for tumor tissue dissociation, with no impact on enzymolysis efficiency. The impact on the viability of tumor cells is minimal: Experimental data shows that the difference in cell viability after dissociating breast cancer tissue with the two media is ≤5%. The medium can be selected based on the existing inventory in the laboratory without deliberate replacement. If tumor cell culture is required in subsequent steps, it is recommended to prioritize the medium consistent with the culture system to reduce the cost of cell adaptation.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e6.    Q: Step 12 mentions that \"BA3311 Red Blood Cell Lysis Buffer can be used to remove red blood cells\". After which step should this operation be performed? What should be noted during lysis to avoid damaging tumor cells?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: This operation should be performed after Step 10 (after two washes) and before Step 11 (before resuspension). Specific operation: After discarding the supernatant in Step 10, add 1mL of BA3311 Red Blood Cell Lysis Buffer, incubate at 4℃ for 5 minutes (to avoid damaging tumor cells due to room-temperature incubation), then centrifuge at 4℃, 300×g for 5 minutes, discard the supernatant, and resuspend with 5mL of PBS containing 5% FBS (add an additional washing step), then proceed to Step 11. Precautions: ① The lysis time should not exceed 8 minutes; the 4℃ environment can reduce the toxicity of the lysis buffer to tumor cells. ② If there are too many red blood cells (such as in blood-rich tumor tissues), lysis can be repeated once, but an additional PBS washing step is required to avoid residual lysis buffer affecting subsequent experiments (e.g., cell capture efficiency in single-cell sequencing).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e7.    Q: The tumor tissue dissociation solution needs to be stored at -20℃ with a validity period of two years. If the ice pack melts during transportation and the reagent is left at 4℃ for 3 hours, can it still be used? What impact does repeated freezing and thawing have?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: It can still be used after being left at 4℃ for 3 hours, but it must be immediately returned to -20℃ and fully mixed before use. The enzymes (such as collagenase and neutral protease) in the dissociation solution lose ≤10% of their activity when placed at 4℃ for a short period (≤3 hours), which does not affect the dissociation effect. Repeated freezing and thawing will lead to a significant decrease in enzyme activity: Each freeze-thaw cycle reduces enzyme activity by 12%-18%; after more than 3 freeze-thaw cycles, the activity is less than 50%, which cannot effectively decompose the extracellular matrix of tumor tissue, resulting in a large number of tissue blocks after dissociation and even failure to obtain a single-cell suspension. It is recommended that after receiving the kit, aliquot the 2×1.25mL dissociation solution into 250μL\/tube (each tube corresponds to one experimental dose), seal it, store at -20℃, and take one tube for each experiment to avoid repeated freezing and thawing.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e8.    Q: After centrifugation in Steps 8 and 9, the supernatant needs to be discarded. If many suspended tumor cells are still found in the supernatant after centrifugation, how to deal with it? Can it be improved by extending the centrifugation time or increasing the rotation speed?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The presence of suspended tumor cells in the supernatant is mostly caused by improper centrifugation parameters or insufficient cell precipitation. Treatment method: Collect the supernatant and re-centrifuge at 4℃, 350×g for 8 minutes (increase the rotation speed by 50×g and extend the time by 3 minutes compared with the original parameters). If there are still a small number of suspended cells after centrifugation, collect the supernatant again and repeat the centrifugation once to ensure full cell recovery. It is not recommended to excessively increase the rotation speed (\u0026gt;400×g) or extend the time (\u0026gt;10 minutes): Excessively high rotation speed will squeeze tumor cells, leading to cell membrane rupture and a 20%-25% decrease in viability; an excessively long time has little impact on cell viability but will increase the experimental time. Effective improvement can be achieved by operating according to the adjusted parameters mentioned above.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e9.    Q: After quality control, it is required to \"carry out subsequent experiments immediately\". If subsequent experiments cannot be conducted immediately, can the prepared tumor cell suspension be stored for a short period? What are the restrictions on storage conditions and time?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Short-term storage is possible under the conditions of sealed storage in a 4℃ refrigerator for no more than 1 hour, with repeated shaking avoided. During storage, adjust the cell concentration to 1×10⁶-1×10⁷ cells\/mL using PBS containing 5% FBS, and place it in a low-adhesion centrifuge tube to prevent cell loss due to adhesion. After 1 hour, the viability of tumor cells will decrease significantly (10%-12% per hour), and cell aggregation is prone to occur; if stored for more than 3 hours, the cell viability may be lower than 50%, making it unsuitable for single-cell sequencing or cell culture. Before use, re-quality inspection is required, and only cells with viability ≥65% and single-cell ratio ≥75% can be used. At the same time, gently pipette 5-8 times to disperse slightly aggregated cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"FireGene","offers":[{"title":"2 reactions\/kit","offer_id":47833413255380,"sku":"FG-BA3320-2rxns","price":89.0,"currency_code":"USD","in_stock":true},{"title":"10 reactions\/kit","offer_id":46299523776724,"sku":"FG-BA3320-10rxns","price":359.0,"currency_code":"USD","in_stock":true},{"title":"50 reactions\/kit","offer_id":47707460796628,"sku":"FG-BA3320-50rxns","price":859.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/files\/BA3320.png?v=1774246586"},{"product_id":"cochlea-dissociation-kit-fg-ba3321","title":"FireGene Cochlea Dissociation Kit - Inner Ear Cell Prep","description":"\u003ch3 id=\"overview\"\u003eOverview\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Cochlea Dissociation Kit\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eis a specialized enzymatic solution optimized to isolate single cells from delicate cochlear tissue. Designed for use in single-cell sequencing and auditory research, this kit ensures high-viability cell suspensions for advanced studies in hearing loss, balance disorders, and neurobiology.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch3 id=\"background-information\"\u003eBackground Information\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eDriven by Clinical and Scientific Research Needs:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eUnderstanding inner ear biology and hearing mechanisms requires accurate dissociation of cochlear tissue into single cells.\u003c\/li\u003e\n\u003cli\u003eTraditional methods often fail to isolate\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ehair cells and auditory neurons\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003ewithout damage.\u003c\/li\u003e\n\u003cli\u003eThe kit supports:\n\u003cul\u003e\n\u003cli\u003eResearch on\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eage-related hearing loss, Ménière’s disease\u003c\/strong\u003e, and genetic hearing impairments.\u003c\/li\u003e\n\u003cli\u003eIdentification of gene expression profiles linked to\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ehair cell degeneration and regeneration\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eDevelopment of\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003etargeted therapies\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003efor restoring hearing and treating\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ebalance dysfunction\u003c\/strong\u003e.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBackground of Technological Development:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eMechanical or chemical dissociation methods often compromise cell integrity and yield.\u003c\/li\u003e\n\u003cli\u003eThis kit employs:\n\u003cul\u003e\n\u003cli\u003eA\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eprecise enzymatic digestion strategy\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003ethat matches the biochemical makeup of cochlear ECM.\u003c\/li\u003e\n\u003cli\u003eCarefully tuned\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eenzyme types, concentrations, and reaction times\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eA robust protocol to achieve\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eefficient, reproducible cell release\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003ewith excellent viability.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch3 id=\"detection-principle\"\u003eDetection Principle\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eBased on a\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003esynergistic enzymatic digestion process\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003etailored to the cochlea:\n\u003cul\u003e\n\u003cli\u003eCochlear tissue is\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003efinely sectioned\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003efor maximal enzyme exposure.\u003c\/li\u003e\n\u003cli\u003eReagents are added in sequence under\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003econtrolled temperature and timing\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eThe enzymes act to\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003edegrade ECM components and loosen intercellular adhesions\u003c\/strong\u003e.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003eFinal Output:\n\u003cul\u003e\n\u003cli\u003eA\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eclean, viable single-cell suspension\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eideal for scRNA-seq, auditory cell profiling, and regenerative studies.\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 id=\"detection-principle\"\u003eSpecifications\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eSingle-cell sequencing, cell culture or other cell-related detections\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eCompatible Sample Types\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eCochlea tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eSupported Instruments\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eWater bath, horizontal centrifuge, cell counter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e-20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eShelf-life\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 id=\"detection-principle\"\u003eKit Components\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"color: rgb(0, 0, 0); background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e10 Reactions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100.179%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40.0716%; font-weight: bold; padding: 8px; border: 1px solid rgb(221, 221, 221);\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 59.9284%; font-weight: bold; padding: 8px; border: 1px solid rgb(221, 221, 221);\"\u003e10 Reactions\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 40.0716%;\"\u003e\u003cspan\u003eCochlea DS (Cochlea Dissociation Solution)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 59.9284%;\"\u003e2 × 1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"color: rgb(0, 0, 0); background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e50 Reactions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 60%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003e50 Reactions\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e\u003cspan\u003eCochlea DS (Cochlea Dissociation Solution)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e10 × 1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eProduct FAQ\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e1.    Q: Is this kit only suitable for fresh cochlear tissue? Is it effective for cryopreserved and resuscitated cochlear tissue?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The kit is mainly suitable for fresh mammalian cochlear tissue (e.g., cochlear tissue from mice, rats, and humans). It should be used with caution for cryopreserved and resuscitated cochlear tissue: Sensitive cells in the cochlea (such as hair cells and supporting cells) are prone to rupture due to ice crystal damage during cryopreservation. After resuscitation, the viability of dissociated cells will be 25%-35% lower than that of fresh tissue. Pre-quality inspection via trypan blue staining is required (viability ≥55% to be usable).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e2.    Q: The instruction manual requires cutting 200mg of fresh cochlear tissue. Will insufficient tissue quantity (e.g., 80mg) or excessive tissue quantity (e.g., 250mg) affect the dissociation effect? How to adjust the operation?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Yes, it will affect the effect. ① Insufficient tissue quantity (\u0026lt;150mg): The content of target cells (e.g., hair cells) in cochlear tissue is inherently low. Insufficient quantity will result in an extremely low final single-cell yield (less than 40% of the normal amount), and the cell pellet will be unobvious during subsequent centrifugation and stratification, easily leading to collection omission. It is recommended to combine multiple 80mg cochlear tissue samples (to a total weight of 180-200mg) and add the dissociation solution according to the conventional dosage to ensure the total number of target cells meets experimental requirements. ② Excessive tissue quantity (\u0026gt;220mg): The space in a 5mL centrifuge tube is limited, so the tissue cannot be fully minced and dispersed. The enzymolysis solution cannot act evenly on all cells, leading to incomplete dissociation of local tissue and over-digestion of local cells. The tissue should be split into two tubes for processing, with each tube containing 180-200mg of tissue and corresponding to 240μL of dissociation solution + 2760μL of RPMI 1640 medium, to avoid the impact of tissue crowding on enzymolysis efficiency.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e3.    Q: Cochlear tissue contains sensitive cells such as hair cells, and the digestion time in Step 3 is 0.5-2 hours. How to determine the optimal digestion time? What impact will improper digestion time have on sensitive cells?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The optimal digestion time needs to be dynamically determined through \"microscopic quality inspection\", and observation is recommended every 15 minutes: ① When more than 80% of the cells in the field of view are in a single dispersed state, there are no obvious clusters of more than 5 cells, and trypan blue staining shows viability ≥70%, terminate digestion immediately. ② If hair cells (columnar in shape with obvious cilia) are observed to start swelling or rupturing, digestion must be terminated even if the minimum digestion time (0.5 hours) has not been reached. Insufficient digestion time: There are many tissue debris, and target cells such as hair cells are still wrapped in the tissue, resulting in a low single-cell yield. Excessive digestion time: Sensitive cells (such as hair cells and spiral ganglion cells) will have their cell membranes ruptured due to over-enzymolysis, leading to a sharp drop in viability. The proportion of target cells in subsequent experiments (e.g., single-cell sequencing) will be less than 30%, which cannot meet research needs.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e4.    Q: Steps 5 and 6 require filtration with a 70μm cell sieve and rinsing the centrifuge tube 3 times. What impact will omitting one rinsing step or replacing with a 50μm\/100μm sieve have on the cochlear cell suspension?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Omitting one rinsing step will cause approximately 1\/3 of the target cochlear cells (e.g., hair cells, supporting cells) to remain on the inner wall of the centrifuge tube, reducing the final yield by more than 30%. Cochlear cells are inherently small in quantity and easily adsorbed on the tube wall, so 3 rinsing steps are crucial to ensure full cell recovery. A 50μm or 100μm sieve cannot be used as a replacement: ① The 50μm sieve has too small a pore size. Although it can filter tissue debris, it will retain some smaller cochlear cells (e.g., spiral ganglion cells, with a diameter of approximately 8-12μm), leading to the loss of target cells. ② The 100μm sieve has too large a pore size and cannot effectively filter undigested cochlear tissue debris (e.g., basement membrane debris). These debris will enter subsequent steps along with the cells, interfering with the accuracy of cell counting and potentially clogging the microchannels of the single-cell sequencing chip, affecting the experimental process.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e5.    Q: The instruction manual mentions that \"DMEM medium can replace RPMI 1640 medium\". After replacement, is it necessary to adjust centrifugation parameters or digestion time? Is there any difference in the impact of the two media on the viability of sensitive cochlear cells (e.g., hair cells)?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: There is no need to adjust centrifugation parameters (still 4℃, 300×g for 5 minutes) or digestion time after replacement. Both DMEM and RPMI 1640 are common basal media for mammalian cells. Although they differ in glucose and amino acid content, both can provide a suitable osmotic pressure (280-320mOsm\/kg) and pH (7.2-7.4) for cochlear tissue dissociation, with no impact on enzymolysis efficiency. The impact on the viability of sensitive cochlear cells is minimal: Experimental data shows that after dissociating mouse cochlear tissue with the two media, the difference in hair cell viability is ≤6%, and the difference in the viability of supporting cells and nerve cells is ≤5%. The medium can be selected based on the existing inventory in the laboratory. If cochlear cell culture is required in subsequent steps, it is recommended to prioritize the medium consistent with the culture system to reduce the stress response of cells due to environmental changes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e6.    Q: The cochlear tissue dissociation solution needs to be stored at -20℃. If the ice pack melts during transportation and the reagent is left at 4℃ for 2 hours, can it still be used? What impact does repeated freezing and thawing have on the enzyme activity in the dissociation solution?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The reagent can still be used after being left at 4℃ for 2 hours, but it must be immediately returned to -20℃ and fully mixed before use. The enzymes (e.g., collagenase, hyaluronidase) in the dissociation solution lose ≤10% of their activity when stored at 4℃ for a short period (≤2 hours), and can still effectively destroy the extracellular matrix of cochlear tissue without affecting the dissociation effect. Repeated freezing and thawing will lead to a significant decrease in enzyme activity: Each freeze-thaw cycle reduces enzyme activity by 12%-15%; after more than 3 freeze-thaw cycles, the activity is less than 50%, which cannot fully decompose the fiber components in cochlear tissue. After dissociation, there will still be a large amount of tissue debris, and the release of target cells (e.g., hair cells) will decrease by more than 60%, which cannot meet experimental needs. It is recommended that after receiving the kit, aliquot the 2×1.25mL dissociation solution into 250μL\/tube (each tube corresponds to one experimental dose), seal it, store at -20℃, and take one tube for each experiment to avoid repeated freezing and thawing.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e7.    Q: The centrifugation parameters for Steps 8 and 9 are \"4℃, 300×g for 5 minutes\". If only a vertical centrifuge is available in the laboratory or the centrifugation speed is incorrect (e.g., 200×g, 400×g), what impact will this have on cochlear cells? Can room-temperature centrifugation replace 4℃ centrifugation?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: A vertical centrifuge cannot replace a horizontal centrifuge. The direction of centrifugal force of a vertical centrifuge is perpendicular to the centrifuge tube, which will cause uneven precipitation of cochlear cells, easily resulting in loose precipitation or adhesion to the tube wall. Sensitive cells (e.g., hair cells) are prone to being sucked away when discarding the supernatant; a horizontal centrifuge allows cells to precipitate evenly at the bottom of the tube, facilitating accurate collection. Impacts of incorrect parameters: ① Speed of 200×g: Insufficient centrifugal force causes cochlear cells (especially hair cells) to fail to precipitate fully and be lost with the supernatant, reducing the yield by more than 45%. ② Speed of 400×g: Excessive centrifugal force squeezes fragile hair cells and nerve cells, leading to cell membrane rupture and a 30%-35% decrease in viability. Room-temperature centrifugation cannot replace 4℃ centrifugation. Room temperature accelerates the metabolism of cochlear cells, and the residual enzymolysis solution has enhanced activity at room temperature, further damaging sensitive cells. Centrifugation at 4℃ can maintain a low metabolic state of cells, reduce viability loss, and ensure the quality of target cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e8.    Q: After quality control, it is required to \"carry out subsequent experiments immediately\". If subsequent experiments cannot be conducted immediately, can the prepared cochlear single-cell suspension be stored for a short period? What are the strict restrictions on storage conditions and time?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Short-term storage is possible, but the conditions are extremely strict: It must be stored in a sealed container at 4℃ in the dark for no more than 30 minutes, and repeated shaking or vibration must be avoided throughout the process. During storage, adjust the cell concentration to 1×10⁶-1×10⁷ cells\/mL using PBS containing 5% FBS, and place it in a low-adhesion centrifuge tube to prevent cell adhesion or damage from mechanical force. After 30 minutes, the viability of sensitive cochlear cells (e.g., hair cells) will decrease rapidly (15%-20% every 30 minutes), and cell aggregation is prone to occur; if stored for more than 1 hour, the viability of hair cells may be lower than 50%, making it unsuitable for high-precision experiments such as single-cell sequencing and cell function detection. Before use, re-conduct trypan blue staining quality inspection; only cells with viability ≥65% and single-cell ratio ≥80% can be used. At the same time, gently pipette 3-5 times (avoid violent pipetting to damage hair cell cilia) to disperse slightly aggregated cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e9.    Q: Compared with the \u003ca href=\"https:\/\/firegene.com\/products\/multi-tissue-dissociation-kit-fg-ba3303?_pos=2\u0026amp;_sid=0c1766ce1\u0026amp;_ss=r\"\u003emulti tissue dissociation kit of this brand (FG-BA3303)\u003c\/a\u003e, what are the core advantages of this cochlear-specific kit? Can \u003ca href=\"https:\/\/firegene.com\/products\/multi-tissue-dissociation-kit-fg-ba3303?_pos=2\u0026amp;_sid=0c1766ce1\u0026amp;_ss=r\"\u003eFG-BA3303\u003c\/a\u003e be used as a replacement for this product to dissociate cochlear tissue?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The core advantages lie in the \"optimization of the enzymolysis system for the characteristics of cochlear tissue\": ① Lower enzyme concentration (the enzyme concentration of FG-BA3321 dissociation solution is only 60% of that of \u003ca href=\"https:\/\/firegene.com\/products\/multi-tissue-dissociation-kit-fg-ba3303?_pos=2\u0026amp;_sid=0c1766ce1\u0026amp;_ss=r\"\u003eFG-BA3303\u003c\/a\u003e), avoiding over-digestion and damage to sensitive cells. ② Addition of hair cell protective components (e.g., low-concentration antioxidants) can reduce oxidative stress damage to hair cells during dissociation, and the hair cell recovery rate is more than 40% higher than that of \u003ca href=\"https:\/\/firegene.com\/products\/multi-tissue-dissociation-kit-fg-ba3303?_pos=2\u0026amp;_sid=0c1766ce1\u0026amp;_ss=r\"\u003eFG-BA3303\u003c\/a\u003e. ③ Better adaptability to enzymolysis time, which can balance tissue dissociation efficiency and cell viability without significant adjustments. \u003ca href=\"https:\/\/firegene.com\/products\/multi-tissue-dissociation-kit-fg-ba3303?_pos=2\u0026amp;_sid=0c1766ce1\u0026amp;_ss=r\"\u003eFG-BA3303\u003c\/a\u003e cannot be used as a replacement. \u003ca href=\"https:\/\/firegene.com\/products\/multi-tissue-dissociation-kit-fg-ba3303?_pos=2\u0026amp;_sid=0c1766ce1\u0026amp;_ss=r\"\u003eFG-BA3303\u003c\/a\u003e is a general-purpose kit, and its enzyme concentration and components are designed for ordinary tissues. When used for cochlear tissue, it is prone to massive death of hair cells and spiral ganglion cells, with viability less than 30%, and incomplete tissue dissociation, making it impossible to obtain a single-cell suspension that meets experimental needs. The cochlear-specific FG-BA3321 kit must be used.\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"2 reactions\/kit","offer_id":47833420759252,"sku":"FG-BA3321-2rxns","price":149.0,"currency_code":"USD","in_stock":true},{"title":"10 reactions\/kit","offer_id":46299523809492,"sku":"FG-BA3321-10rxns","price":589.0,"currency_code":"USD","in_stock":true},{"title":"50 reactions\/kit","offer_id":47707479441620,"sku":"FG-BA3321-50rxns","price":1949.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/files\/BA3321.png?v=1774247199"},{"product_id":"kidney-dissociation-kit-fg-ba3322","title":"FireGene Kidney Dissociation Kit - Enzymatic Single-Cell Isolation","description":"\u003ch3 id=\"overview\"\u003eOverview\u003c\/h3\u003e\n\u003cp\u003eThe\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eFireGene Kidney Dissociation Kit\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eis a precision-engineered enzymatic system for the efficient isolation of single cells from kidney tissue. Designed to support cutting-edge research into renal function and pathology, this kit delivers high-viability suspensions for single-cell sequencing, flow cytometry, and biomarker discovery.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch3 id=\"background-information\"\u003eBackground Information\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eDriven by Clinical and Scientific Research Needs:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eKidney diseases such as\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eacute kidney injury (AKI)\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eand\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003echronic kidney disease (CKD)\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003erequire cellular-level investigation to understand their progression.\u003c\/li\u003e\n\u003cli\u003eTraditional dissociation approaches do not capture the full range of renal cell types and often lead to low yield.\u003c\/li\u003e\n\u003cli\u003eThis kit enables:\n\u003cul\u003e\n\u003cli\u003eHigh-resolution profiling of\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003erenal tubule cells, podocytes, immune cells\u003c\/strong\u003e, and more.\u003c\/li\u003e\n\u003cli\u003eDiscovery of biomarkers related to\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003erenal fibrosis, inflammation\u003c\/strong\u003e, and tissue remodeling.\u003c\/li\u003e\n\u003cli\u003eAccelerated development of targeted treatments for nephrological disorders.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBackground of Technological Development:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eTraditional dissociation techniques are limited by\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003elow efficiency and cell damage\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eFireGene addresses these challenges by:\n\u003cul\u003e\n\u003cli\u003eUsing\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003erefined enzymatic formulations\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003ematched to the unique extracellular matrix of kidney tissue.\u003c\/li\u003e\n\u003cli\u003eTuning\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eenzyme concentration, incubation time, and buffer composition\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003efor optimal results.\u003c\/li\u003e\n\u003cli\u003eEnsuring\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003emaximum recovery\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eof viable, functional cells ready for downstream workflows.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch3 id=\"detection-principle\"\u003eDetection Principle\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eUtilizes a\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003estepwise enzymatic digestion process\u003c\/strong\u003e:\n\u003cul\u003e\n\u003cli\u003eKidney tissue is\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003efinely chopped\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eand exposed to carefully formulated enzymatic reagents.\u003c\/li\u003e\n\u003cli\u003eReagents act in synergy under\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003econtrolled temperature and timing conditions\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eThe enzymes degrade the\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eextracellular matrix and intercellular connections\u003c\/strong\u003e, enabling complete tissue dissociation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003eOutcome:\n\u003cul\u003e\n\u003cli\u003eA\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ehigh-purity, viable single-cell suspension\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003esuitable for scRNA-seq, functional assays, and renal disease modeling.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 id=\"detection-principle\"\u003eSpecifications\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eSingle-cell sequencing, cell culture or other cell-related detections\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eCompatible Sample Types\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eKidney tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eSupported Instruments\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eWater bath, horizontal centrifuge, cell counter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e-20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eShelf-life\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 id=\"detection-principle\"\u003eKit Components\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e10 Reactions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 60%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003e10 Tests\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003eKDS\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e2 × 1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e50 Reactions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 60%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003e50 Tests\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003eKDS\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e10 × 1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eProduct FAQ\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eQ: Is this kit only suitable for fresh mammalian kidney tissue? Are there differences in dissociation effects on tissues from different parts of the kidney (e.g., cortex, medulla, renal pelvis)? Can it be used for cryopreserved and resuscitated kidney tissue?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: The kit is only suitable for fresh mammalian kidney tissue. There are differences in dissociation effects on tissues from different parts of the kidney: The renal cortex (containing a large number of glomeruli and renal tubules) has low fiber content and high enzymolysis efficiency, and high-viability single cells can be obtained within 20-30 minutes; the medulla and renal pelvis tissues contain more collagen fibers, so the enzymolysis time needs to be extended to 30-40 minutes, and quality inspection must be conducted every 5 minutes to avoid over-digestion.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e2.    Q: The instruction manual requires cutting 200mg of fresh kidney tissue. Will insufficient tissue quantity (e.g., 80mg) or excessive tissue quantity (e.g., 300mg) affect the dissociation effect? How to adjust the operation?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Yes, it will affect the effect. ① Insufficient tissue quantity (\u0026lt;150mg): The total number of target kidney cells (such as glomerular endothelial cells and renal tubular epithelial cells) is small, and the cell pellet is not obvious during subsequent centrifugation and collection, which easily leads to omission and a yield reduction of more than 50%. It is recommended to combine multiple 80mg tissue samples (to a total weight of 180-200mg) and add 3mL of dissociation solution according to the conventional dosage to ensure the total number of target cells meets the experimental requirements. ② Excessive tissue quantity (\u0026gt;250mg): The space in a 5mL centrifuge tube is limited, and the dissociation solution cannot fully wrap all tissues, which easily causes local over-enzymolysis (e.g., tissue at the bottom of the tube) and local incomplete dissociation (e.g., tissue at the liquid surface). The tissue should be split into two tubes for processing, with each tube containing 180-220mg of tissue and corresponding to 3mL of dissociation solution, to avoid the impact of tissue crowding on enzymolysis efficiency.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e3.    Q: The enzymolysis time in Step 3 is 20-40 minutes. How to determine the initial enzymolysis time based on the state of kidney tissue (e.g., kidney tissue from young\/adult animals, healthy\/diseased kidneys)? What consequences will improper enzymolysis time cause?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The initial enzymolysis time needs to be adjusted based on tissue characteristics: ① Kidney tissue from young animals (e.g., kidneys of newborn mice) is relatively delicate, so the initial enzymolysis time is set to 20-25 minutes; kidney tissue from adult animals has denser fibers, so the initial enzymolysis time is set to 25-30 minutes. ② The enzymolysis efficiency of healthy kidney tissue is stable, and operation can be carried out within the above time range; diseased kidney tissue (e.g., fibrotic kidneys) contains a large number of abnormal collagen fibers, so the initial enzymolysis time needs to be extended to 30-35 minutes, and the cell dispersion state must be closely observed. Insufficient enzymolysis time: The tissue is not fully dissociated, and a large number of clusters of more than 10 cells can be seen under the microscope, with a single-cell yield of less than 50%. Excessive enzymolysis time: Sensitive kidney cells (such as glomerular cells) will have their cell membranes ruptured due to over-enzymolysis, with viability reduced to less than 50%, and the proportion of target cells in subsequent experiments is less than 30%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e4.    Q: Steps 5 and 6 require filtration with a 70μm cell sieve and rinsing of the centrifuge tube to collect a total of 9mL of filtrate. What impact will omitting the rinsing step or replacing with a 50μm\/100μm sieve have on the experimental results?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Omitting the rinsing step will cause approximately 1\/3 of kidney cells to remain on the inner wall of the centrifuge tube, resulting in a final yield reduction of more than 30%. Kidney cells (especially renal tubular epithelial cells) are easily adsorbed on the tube wall, so rinsing is the key to ensuring full cell recovery. A 50μm or 100μm sieve cannot be used as a replacement: ① The 50μm sieve has a too-small pore size. Although it can filter tissue debris, it will retain some smaller kidney cells (such as glomerular mesangial cells, with a diameter of about 8-10μm), leading to the loss of target cells. ② The 100μm sieve has a too-large pore size and cannot effectively filter undigested kidney tissue debris (such as renal interstitial fiber debris). These debris will enter subsequent steps along with cells, interfering with the accuracy of cell counting and may even block the microchannels of the single-cell sequencing chip.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e5.    Q: The instruction manual mentions that \"DMEM medium can replace RPMI 1640 medium\". After replacement, is it necessary to adjust centrifugation parameters or enzymolysis time? Is there any difference in the impact of the two media on the viability of kidney cells?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: There is no need to adjust centrifugation parameters (still 4℃, 300×g for 5 minutes) or enzymolysis time after replacement. Both DMEM and RPMI 1640 are common basal media for mammalian cells. Although they differ in glucose and amino acid content, both can provide a suitable osmotic pressure (280-320mOsm\/kg) and pH (7.2-7.4) for kidney tissue dissociation, with no impact on enzymolysis efficiency. The impact on the viability of kidney cells is minimal: Experimental data shows that after dissociating mouse renal cortex tissue with the two media, the difference in the viability of renal tubular epithelial cells is ≤5%, and the difference in the viability of glomerular cells is ≤6%. The medium can be selected based on the existing inventory in the laboratory; if kidney cell culture is required in subsequent steps, it is recommended to prioritize the medium consistent with the culture system to reduce the cost of cell adaptation.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e6.    Q: Step 11 mentions that \"\u003ca href=\"https:\/\/firegene.com\/products\/red-blood-cell-lysis-kit-fg-ba3311?_pos=5\u0026amp;_sid=9344ad068\u0026amp;_ss=r\"\u003eFG-BA3311 Red Blood Cell Lysis Buffer\u003c\/a\u003e can be used to remove red blood cells\". After which step should this operation be performed? What should be paid special attention to during lysis to avoid damaging kidney cells?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: It should be performed after Step 9 (after two washes) and before Step 10 (before resuspension). At this time, red blood cells and kidney cells have been initially separated, which can reduce the impact of the lysis buffer on target cells. Specific operation: After discarding the supernatant in Step 9, add 1mL of \u003ca href=\"https:\/\/firegene.com\/products\/red-blood-cell-lysis-kit-fg-ba3311?_pos=5\u0026amp;_sid=9344ad068\u0026amp;_ss=r\"\u003eFG-BA3311 Red Blood Cell Lysis Buffer\u003c\/a\u003e, incubate at 4℃ for 5 minutes (room-temperature incubation is strictly prohibited, as room temperature will accelerate the toxicity of the lysis buffer to kidney cells), then centrifuge at 4℃, 300×g for 5 minutes, discard the supernatant, resuspend with 5mL of PBS containing 5% FBS (add an additional wash), and then proceed to Step 10. Precautions: ① The lysis time should not exceed 8 minutes; the 4℃ environment can minimize the damage of the lysis buffer to renal tubular epithelial cells and glomerular cells. ② If there are many red blood cells (e.g., in vascular-rich areas of the renal cortex), lysis can be repeated once, but an additional two washes with PBS are required to avoid residual lysis buffer affecting subsequent experiments (e.g., non-specific signals in cell immunofluorescence staining).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e7.    Q: The kidney tissue dissociation solution needs to be stored at -20℃ with a validity period of two years. If the ice pack melts during transportation and the reagent is left at 4℃ for 3 hours, can it still be used? What impact does repeated freezing and thawing have on the enzyme activity of the dissociation solution?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: It can still be used after being left at 4℃ for 3 hours, but it must be immediately returned to -20℃ and fully mixed before use. The enzymes (such as collagenase and neutral protease) in the dissociation solution lose ≤10% of their activity when stored at 4℃ for a short period (≤3 hours), and can still effectively destroy the extracellular matrix of kidney tissue without affecting the dissociation effect. Repeated freezing and thawing will lead to a significant decrease in enzyme activity: Each freeze-thaw cycle reduces enzyme activity by 12%-15%; after more than 3 freeze-thaw cycles, the activity is less than 50%, which cannot fully decompose the fiber components in kidney tissue. After dissociation, there will still be a large amount of tissue debris, and the release of target cells (such as renal tubular epithelial cells) will decrease by more than 60%, which cannot meet experimental needs. It is recommended that after receiving the kit, aliquot the 30mL dissociation solution into 3mL\/tube (each tube corresponds to one experimental dose), seal it, store at -20℃, and take one tube for each experiment to avoid repeated freezing and thawing.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e8.    Q: The centrifugation parameters for Steps 7 and 9 are \"4℃, 300×g for 5 minutes\". If only a vertical centrifuge is available in the laboratory or the centrifugation speed is incorrect (e.g., 200×g, 400×g), what impact will this have on kidney cells? Can room-temperature centrifugation replace 4℃ centrifugation?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: A vertical centrifuge cannot replace a horizontal centrifuge. The direction of centrifugal force of a vertical centrifuge is perpendicular to the centrifuge tube, which will cause uneven precipitation of kidney cells, easily resulting in loose precipitation or adhesion to the tube wall. Sensitive cells (e.g., glomerular cells) are prone to being sucked away when discarding the supernatant; a horizontal centrifuge allows cells to precipitate evenly at the bottom of the tube, facilitating accurate collection. Impacts of incorrect parameters: ① Speed of 200×g: Insufficient centrifugal force causes kidney cells (especially renal tubular epithelial cells) to fail to precipitate fully and be lost with the supernatant, reducing the yield by more than 45%. ② Speed of 400×g: Excessive centrifugal force squeezes fragile glomerular cells and renal tubular cells, leading to cell membrane rupture and a 30%-35% decrease in viability. Room-temperature centrifugation cannot replace 4℃ centrifugation. Room temperature accelerates the metabolism of kidney cells, and the residual enzymolysis solution has enhanced activity at room temperature, further damaging sensitive cells. Centrifugation at 4℃ can maintain a low metabolic state of cells, reduce viability loss, and ensure the quality of target cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e9.    Q: After quality control, it is required to \"carry out subsequent experiments immediately\". If subsequent experiments cannot be conducted immediately, can the prepared kidney single-cell suspension be stored for a short period? What are the strict restrictions on storage conditions and time?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Short-term storage is possible, but the conditions are extremely strict: It must be stored sealed at 4℃ in the dark for no more than 1 hour, and repeated shaking or vibration must be avoided throughout the process. During storage, adjust the cell concentration to 1×10⁶-1×10⁷ cells\/mL using PBS containing 5% FBS, and place it in a low-adhesion centrifuge tube to prevent cell adhesion or damage from mechanical force. After 1 hour, the viability of sensitive kidney cells (e.g., glomerular endothelial cells) will decrease rapidly (10%-15% per hour), and cell aggregation is prone to occur; if stored for more than 2 hours, the viability of glomerular cells may be lower than 50%, making it unsuitable for high-precision experiments such as single-cell sequencing and cell function detection. Before use, re-conduct trypan blue staining quality inspection; only cells with viability ≥65% and single-cell ratio ≥80% can be used. At the same time, gently pipette 5-8 times (avoid violent pipetting to damage cell structure) to disperse slightly aggregated cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e10.    Q: Compared with the general \u003ca href=\"https:\/\/firegene.com\/products\/multi-tissue-dissociation-kit-fg-ba3303?_pos=1\u0026amp;_sid=3315d617b\u0026amp;_ss=r\"\u003etissue dissociation kit of this brand (FG-BA3303\u003c\/a\u003e), what are the core advantages of this kidney-specific kit? Can FG-BA3303 be used as a replacement for this product to dissociate kidney tissue?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The core advantages lie in the \"optimization of the enzymolysis system for the characteristics of kidney tissue\": ① Better adaptability of enzyme components, containing kidney tissue-specific fiber-degrading enzymes (such as low-concentration hyaluronidase), which can accurately degrade renal interstitial collagen fibers, and the recovery rate of renal tubular epithelial cells is more than 35% higher than that of FG-BA3303. ② Milder enzyme concentration (the enzyme concentration of FG-BA3322 dissociation solution is only 70% of that of FG-BA3303), avoiding over-digestion and damage to sensitive cells such as glomeruli, and the cell viability is 20%-25% higher than that of FG-BA3303. ③ Better adaptability to enzymolysis time, which can balance tissue dissociation efficiency and cell viability without significant adjustments. FG-BA3303 cannot be used as a replacement. FG-BA3303 is a general-purpose kit, and its enzyme concentration and components are designed for ordinary tissues. When used for kidney tissue, it is prone to massive death of glomerular cells, with viability less than 40%, and incomplete tissue dissociation, making it impossible to obtain a single-cell suspension that meets experimental needs. The kidney-specific FG-BA3322 kit must be used.\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"2 reactions\/kit","offer_id":47833492521172,"sku":"FG-BA3322-2rxns","price":99.0,"currency_code":"USD","in_stock":false},{"title":"10 reactions\/kit","offer_id":46299524235476,"sku":"FG-BA3322-10rxns","price":399.0,"currency_code":"USD","in_stock":true},{"title":"50 reactions\/kit","offer_id":47683164209364,"sku":"FG-BA3322-50rxns","price":1399.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/files\/BA3322.png?v=1774000637"},{"product_id":"liver-dissociation-kit-fg-ba3323","title":"FireGene Liver Dissociation Kit for Hepatic Cell Profiling","description":"\u003ch3 id=\"overview\"\u003eOverview\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Liver Dissociation Kit\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eprovides a powerful enzymatic solution designed to dissociate liver tissue into single-cell suspensions for high-throughput applications. This kit enables researchers to study liver cellular heterogeneity with high viability and efficiency, supporting advanced liver disease research and drug development.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch3 id=\"background-information\"\u003eBackground Information\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eDriven by Clinical and Scientific Research Needs:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSingle-cell analysis of liver tissue is key to understanding diseases like\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ehepatitis\u003c\/strong\u003e,\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003efibrosis\u003c\/strong\u003e, and\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eliver cancer\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eTraditional dissociation methods often fail to preserve fragile liver cell subtypes or yield complete suspensions.\u003c\/li\u003e\n\u003cli\u003eThis kit facilitates:\n\u003cul\u003e\n\u003cli\u003eIdentification of diverse liver cell types, including\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ehepatocytes, Kupffer cells, and stellate cells\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eInvestigation of\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003edisease mechanisms\u003c\/strong\u003e, such as inflammation, fibrosis, and tumor progression.\u003c\/li\u003e\n\u003cli\u003eDiscovery of\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ebiomarkers and therapeutic targets\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003efor liver-related pathologies.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBackground of Technological Development:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eOvercomes limitations of cell damage and inefficiency in mechanical or chemical methods.\u003c\/li\u003e\n\u003cli\u003eFireGene leverages:\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecially selected enzymes\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003etargeting liver extracellular matrix.\u003c\/li\u003e\n\u003cli\u003eOptimization of\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eenzyme type, concentration, and incubation parameters\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eA high-yield workflow for producing\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eviable, reproducible single-cell suspensions\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003efrom liver tissue.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch3 id=\"detection-principle\"\u003eDetection Principle\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eOperates on a\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003etwo-step enzymatic digestion method\u003c\/strong\u003e:\n\u003cul\u003e\n\u003cli\u003eLiver tissue is\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ecut into small fragments\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003efor uniform exposure.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEnzyme reagents\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eare applied under precisely controlled\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003etemperature and time\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003econditions.\u003c\/li\u003e\n\u003cli\u003eThe matrix and cellular junctions are enzymatically degraded.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003eFinal Result:\n\u003cul\u003e\n\u003cli\u003eA\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ehigh-quality, viable single-cell suspension\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eready for applications such as scRNA-seq, immunophenotyping, or cell culture.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 id=\"detection-principle\"\u003eSpecifications\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eSingle-cell sequencing, cell culture or other cell-related detections\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eCompatible Sample Types\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eLiver tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eSupported Instruments\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eWater bath, horizontal centrifuge, cell counter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e-20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eShelf-life\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 id=\"detection-principle\"\u003eKit Components\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e10 Reactions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 60%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003e10 Reactions\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e\u003cspan\u003eLiver DS (Liver Dissociation Solution)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e2 × 1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e50 Reactions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 60%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003e50 Reactions\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e\u003cspan\u003eLiver DS (Liver Dissociation Solution)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e10 × 1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eProduct FAQ\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e1.    Q: During the dissociation process, brown oxidation spots are found on the liver tissue. Will this affect the subsequent cell viability? How to prevent tissue oxidation?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Brown oxidation spots are caused by the oxidation of hemoglobin or cytochromes in liver tissue. They will reduce the local cell viability by 15%-20% and may also interfere with enzymolysis efficiency. Prevention methods: ① Immediately place the tissue in PBS containing 1% ascorbic acid (not included in the kit, need to be prepared separately) after sampling to prevent oxidation; ② Control the time from sampling to adding the dissociation solution within 10 minutes to reduce the exposure time to air; ③ If a small number of oxidation spots have appeared, remove the oxidized area before mincing the tissue to avoid affecting the quality of the overall cell suspension.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e2.    Q: Can the dissociation solution in the kit be mixed with collagenase from other brands? What impact will mixing have on the enzymolysis effect?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Mixing is strictly prohibited. The enzymes in the dissociation solution of this kit have been precisely formulated at a 3:1 ratio and added with a liver tissue-specific stabilizer. Mixing with collagenase from other brands will disrupt the proportional balance of enzymes, which may lead to: ① Excessively strong enzyme activity, causing over-digestion of hepatocytes and reducing viability to below 40%; ② Enzyme activity antagonism, failing to degrade hepatic interstitial fibers, resulting in a tissue block residue rate of over 60%. Even if the dissociation solution of this kit is insufficient, a supplementary package of the same brand (product number: \u003ca href=\"https:\/\/firegene.com\/products\/liver-dissociation-kit-fg-ba3323?_pos=1\u0026amp;_sid=f7c9aba3a\u0026amp;_ss=r\"\u003eFG-BA3323\u003c\/a\u003e) must be purchased separately; random replacement is not allowed.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e3.    Q: A large amount of transparent mucus-like substance appears in the cell suspension after enzymolysis. What causes this, and how to remove the mucus?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The transparent mucus-like substance is formed by the mixture of mucopolysaccharides (such as heparin) in liver tissue and enzymolysis products. It will wrap cells, leading to inaccurate counting, and may also block the cell sieve. Removal method: When filtering with a 70μm cell sieve, rinse the sieve repeatedly with PBS containing 2% FBS for 3 times to ensure the mucus flows out with the filtrate and avoid residue.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e4.    Q: After centrifugation, the cell pellet shows a layered state (pale yellow in the upper layer and dark red in the lower layer). How to collect the target hepatocytes? Does stratification mean low cell purity?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Stratification is caused by the density difference between hepatocytes (density: 1.05-1.07g\/cm³) and red blood cells (density: 1.09g\/cm³). The pale yellow upper layer is hepatocytes, and the dark red lower layer is red blood cells; this does not mean low purity. Collection method: ① Use a 1mL low-adhesion pipette tip close to the liquid surface to first aspirate the pale yellow pellet in the upper layer (accounting for about 60%-70% of the total pellet), which are high-purity hepatocytes (purity ≥85%); ② If a small number of hepatic sinusoidal endothelial cells need to be retained, the middle transition layer (about 10%) can be collected, but subsequent flow sorting is required for further purification to avoid red blood cell contamination, and red blood cell lysis buffer can also be used for treatment.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e5.    Q: After opening the kit, if some reagents (such as washing buffer) cannot be used up in a short time, how do the storage conditions and validity period change after opening?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The storage conditions and validity period of reagents need to be adjusted after opening: ① Dissociation solution: After opening, aliquot into 250μL\/tube, store sealed at -20℃, and the validity period is shortened to 3 months from the opening date to avoid repeated freezing and thawing; ② Washing buffer: After opening, store at 4℃ in the dark, and the validity period is shortened from 2 years to 1 month. Tighten the cap immediately after each use to prevent microbial contamination; ③ Enzymolysis termination solution: After opening, store at 4℃, and the validity period is shortened to 2 months. If turbidity or flocculent precipitates appear, discard immediately and do not use.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e6.    Q: When dissociating liver tissue from juvenile mice (within 7 days of birth), cells tend to aggregate. What causes this, and how to adjust the operating parameters to reduce aggregation?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Hepatocytes from juvenile mice are small in size (about 1\/3 of that from adult mice) and have fragile cell membranes, so they are prone to aggregation due to collision or uneven enzymolysis. Adjustment methods: ① When mincing the tissue, cut it into pieces smaller than 0.5mm³ (half the size of adult mouse tissue) to ensure sufficient enzymolysis; ② Reduce the enzymolysis rotation speed to 15-20 rpm (20-30 rpm for adult mice) to reduce cell collision; ③ After terminating enzymolysis, gently pipette 10 times with a 1mL pipette tip (avoid violent pipetting), then filter with a 40μm cell sieve (70μm for regular use) to remove tiny tissue blocks and reduce the aggregation rate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e7.    Q: For downstream experiments requiring hepatocyte nucleus extraction, can nucleus extraction be performed directly after dissociation with this kit? If yes, after which step should the nucleus extraction operation be carried out?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Nucleus extraction can be performed directly, but it must be carried out after a specific step. Optimal timing: After Step 10 (after two washes) and before Step 11 (before resuspension). Specific operation: ① After discarding the supernatant in Step 10, add 1mL of nucleus extraction buffer (need to be prepared separately, such as NP-40 buffer), incubate on ice for 10 minutes to lyse the cell membrane; ② Centrifuge at 4℃, 500×g for 8 minutes (200×g higher than the regular centrifugation speed) to collect the nuclear pellet; ③ Resuspend the nuclear pellet with PBS containing 0.5% BSA, and follow the regular nucleus extraction steps for subsequent operations. Note: Nucleus extraction cannot be performed before or during enzymolysis, otherwise the enzymolysis solution will damage the nuclear structure.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e8.    Q: During enzymolysis, the temperature fluctuation of the water bath exceeds ±1℃ (e.g., set at 37℃, actual fluctuation between 36-38℃). What impact will this have on the enzymolysis effect? How to stabilize the water bath temperature?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Temperature fluctuation exceeding ±1℃ will cause unstable enzyme activity, which may lead to: ① Decreased enzyme activity during low-temperature periods (36℃), resulting in incomplete tissue dissociation and a 30% increase in cell clumping rate; ② Excessively strong enzyme activity during high-temperature periods (38℃), reducing hepatocyte viability by 25%. Methods to stabilize temperature: ① Use a water bath with constant temperature circulation function instead of a regular water bath; ② Turn on the water bath 30 minutes in advance to preheat, and place the sample only after the temperature stabilizes; ③ After placing the sample, check the temperature every 15 minutes. If the fluctuation exceeds ±0.5℃, adjust the water bath settings in time to ensure constant temperature.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e9.    Q: After dissociation, the cell viability meets the requirements (≥70%), but the adhesion rate after seeding on the culture plate is extremely low (\u0026lt;20%). What causes this, and how to improve the adhesion rate?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Low adhesion rate is mostly caused by the damage of adhesion molecules (such as integrins) on the hepatocyte surface during dissociation, or residual enzymolysis solution components in the cell suspension. Improvement methods: ① In the washing steps of Step 9 and Step 10, replace PBS with DMEM medium containing 5% fetal bovine serum, and extend the centrifugation time to 8 minutes (5 minutes for regular use) to fully remove residual enzymolysis solution; ② Before seeding, coat the culture plate with 10μg\/cm² collagen (not included in the kit, need to be prepared separately), and incubate at 37℃ for 1 hour to enhance cell adhesion ability; ③ After seeding, place the culture plate in the incubator and let it stand for 4 hours. Avoid shaking too early to allow sufficient cell adhesion.\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"2 reactions\/kit","offer_id":47833515131092,"sku":"FG-BA3323-2rxns","price":99.0,"currency_code":"USD","in_stock":false},{"title":"10 reactions\/kit","offer_id":46299524268244,"sku":"FG-BA3323-10rxns","price":399.0,"currency_code":"USD","in_stock":true},{"title":"50 reactions\/kit","offer_id":47707497726164,"sku":"FG-BA3323-50rxns","price":1419.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/files\/BA3323.png?v=1774248025"},{"product_id":"spleen-dissociation-kit-fg-ba3324","title":"FireGene Spleen Dissociation Kit - Immune Cell Analysis Ready","description":"\u003ch3 id=\"overview\"\u003eOverview\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Spleen Dissociation Kit\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eis designed to produce high-viability single-cell suspensions from spleen tissue through a carefully optimized enzymatic protocol. It is ideal for immunological studies, single-cell sequencing, and immune profiling applications, offering exceptional cell recovery and integrity.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch3 id=\"background-information\"\u003eBackground Information\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eDriven by Clinical and Scientific Research Needs:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSingle-cell sequencing of spleen tissue is critical for understanding immune mechanisms and identifying functional cell subsets.\u003c\/li\u003e\n\u003cli\u003eTraditional methods often fail to preserve rare or sensitive immune populations.\u003c\/li\u003e\n\u003cli\u003eThis kit supports:\n\u003cul\u003e\n\u003cli\u003eDetailed profiling of\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eT cells, B cells, dendritic cells, and macrophages\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eInvestigation of immune responses in\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003esepsis, autoimmune disorders\u003c\/strong\u003e, and cancer.\u003c\/li\u003e\n\u003cli\u003eDiscovery of\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eimmunological biomarkers\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eand\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003etherapeutic targets\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003efor translational and clinical research.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBackground of Technological Development:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eMechanical disruption can damage spleen cells and yield inconsistent results.\u003c\/li\u003e\n\u003cli\u003eFireGene improves performance with:\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eEnzyme cocktails tailored\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eto the spleen’s extracellular matrix.\u003c\/li\u003e\n\u003cli\u003eOptimization of\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eincubation times, temperatures, and concentrations\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eHigh reproducibility and\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003esuperior cell viability\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003efor downstream assays.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch3 id=\"detection-principle\"\u003eDetection Principle\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eBased on a\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003esynergistic multi-enzyme digestion process\u003c\/strong\u003e:\n\u003cul\u003e\n\u003cli\u003eSpleen tissue is\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eminced into small sections\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eto enhance reagent access.\u003c\/li\u003e\n\u003cli\u003eEnzymes are added sequentially under\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003econtrolled conditions\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eThe digestion breaks down\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eECM structures and cellular junctions\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eto release single cells.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003eFinal Outcome:\n\u003cul\u003e\n\u003cli\u003eA\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ehigh-quality single-cell suspension\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003esuitable for scRNA-seq, flow cytometry, and immunophenotyping.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 id=\"detection-principle\"\u003eSpecifications\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eSingle-cell sequencing, cell culture or other cell-related detections\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eCompatible Sample Types\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eSpleen tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eSupported Instruments\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eWater bath, horizontal centrifuge, cell counter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e-20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eShelf-life\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 id=\"detection-principle\"\u003eKit Components\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e10 Reactions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 60%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003e10 Reactions\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e\u003cspan\u003eSpleen DS (Spleen Dissociation Solution)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e2 × 1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e50 Reactions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 60%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003e50 Reactions\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e\u003cspan\u003eSpleen DS (Spleen Dissociation Solution)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e10 × 1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eProduct FAQ\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003e1.    Q: When dissociating spleen tissue from juvenile mice (within 14 days of birth), the proportion of immune cells (e.g., lymphocytes) in the cell suspension is found to be extremely low. What causes this, and how to adjust the operation?\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The spleen of juvenile mice is not fully developed, and immune cells are mainly concentrated in the white pulp region. If the tissue is not minced sufficiently, the white pulp fibers remain intact, making it difficult for immune cells to be released. Adjustment methods: ① Mince the spleen tissue into pieces smaller than 0.3mm³ (half the size of adult mouse spleen tissue) to ensure the white pulp structure is disrupted; ② Add RPMI 1640 medium containing 0.1% DNase I (self-prepared) during enzymolysis to prevent immune cell aggregation caused by nucleic acid release; ③ Shorten the enzymolysis time to 10-20 minutes to avoid over-digestion of immune cells. Eventually, the proportion of immune cells can be increased to over 75%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e2.    Q: After dissociation, red blood cells account for more than 60% of the cell suspension. After treatment with BA3311 Red Blood Cell Lysis Buffer, the viability of lymphocytes drops from 85% to 55%. How to optimize the lysis operation to reduce damage to immune cells?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The decrease in viability is mostly due to excessive lysis time or improper temperature. Optimization methods: ① Adjust the incubation temperature of the lysis buffer from room temperature to 4℃ to reduce its toxicity to immune cells; ② Shorten the incubation time to 3-4 minutes (5 minutes for regular operation), and observe under a microscope to terminate the reaction immediately after most red blood cells are lysed; ③ Wash twice with PBS containing 10% FBS after lysis (once for regular operation) to fully remove residual lysis buffer. This can maintain the viability of lymphocytes at over 75%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e3.    Q: When using a hybridization oven for enzymolysis, a large amount of foam appears in the solution in the centrifuge tube, leading to inaccurate subsequent cell counting. What causes this, and how to prevent foam formation?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Foam formation is caused by the reaction between surface proteins of immune cells in the spleen tissue and the enzymolysis solution, as well as excessive rotation speed of the hybridization oven leading to violent liquid oscillation. Prevention methods: ① Reduce the rotation speed of the hybridization oven from 20-30 rpm to 15-20 rpm to minimize liquid impact; ② Add 1 drop of defoamer (e.g., 0.1% polyethylene glycol, self-prepared) to the centrifuge tube before enzymolysis, ensuring the defoamer is non-toxic to cells; ③ If foam has already formed, gently aspirate the surface foam with a 1mL pipette tip before filtration to avoid foam interfering with cell counting.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e4.    Q: After opening the kit, the dissociation solution is stored at -20℃ for 4 months. When used, the enzymolysis efficiency is found to decrease significantly (cell yield is only 50% of the original). Is the dissociation solution invalid? How to determine if the dissociation solution is still usable?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The validity period of the dissociation solution after opening is 3 months when stored at -20℃ (2 years for unopened solution). After 4 months, it has exceeded the validity period, and the enzyme activity may decrease significantly. Judgment methods: ① Mix 50μL of the dissociation solution with a small amount of minced spleen tissue, incubate at 37℃ for 30 minutes. If the tissue pieces do not shrink significantly, it indicates insufficient enzyme activity; ② If the enzyme activity decreases slightly but the solution still needs to be used, extend the enzymolysis time and increase the amount of dissociation solution to 300μL to compensate for the reduced enzyme activity. This can restore the cell yield to approximately 70% of the original.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e5.    Q: During filtration after enzymolysis, severe clogging of the 70μm cell sieve is observed, with extremely slow filtration speed or even no filtration. What causes this, and how to handle the clogged sieve?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Clogging is caused by the mixture of red blood cells, immune cell debris, and undigested fibers in the spleen tissue adhering to the sieve pores. Handling methods: ① Before filtration, let the cell suspension stand for 5 minutes, then aspirate the clearer upper liquid for filtration first to avoid direct clogging by bottom precipitates; ② If the sieve is already clogged, rinse both the front and back of the sieve repeatedly with PBS containing 5% FBS until the pores are unobstructed; ③ In subsequent experiments, mince the tissue more finely (smaller than 0.5mm³) and extend the enzymolysis time by 10 minutes to reduce undigested tissue pieces and prevent sieve clogging again.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e6.    Q: After centrifugation, the cell pellet is loose and flocculent, failing to form a dense pellet, leading to uneven cell dispersion during subsequent resuspension. What causes this, and how to obtain a dense cell pellet?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The loose flocculent pellet is due to the negative charge on the surface of spleen immune cells, which causes mutual repulsion and prevents aggregation. Solutions: ① Increase the centrifugation speed from 300×g to 400×g to enhance centrifugal force; ② Extend the centrifugation time from 5 minutes to 8 minutes to provide sufficient time for cell sedimentation; ③ Add 10μL of 1% polylysine (self-prepared, non-toxic to cells) to the cell suspension before centrifugation to neutralize the surface charge of cells and promote aggregation. This can increase the density of the cell pellet by over 60%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e7.    Q: When using a water bath for enzymolysis, shaking every 3-5 minutes is forgotten. After 1 hour of enzymolysis, unlysed tissue pieces are found at the bottom of the centrifuge tube. How to remedy this?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The unlysed tissue pieces at the bottom are caused by static liquid preventing the enzymolysis solution from contacting the bottom tissue. Remedial methods: ① Immediately take out the centrifuge tube and pipette the bottom tissue pieces repeatedly 10 times to disperse the tissue; ② Add 50μL of spleen tissue dissociation solution and extend the enzymolysis time by 30 minutes; ③ Shake every 2 minutes thereafter to ensure full contact between the enzymolysis solution and the tissue. This can lyse most of the bottom tissue pieces, restoring the cell yield to approximately 80% of the normal level.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e8.    Q: When the dissociated cell suspension is used for single-cell sequencing, the cell debris rate is as high as 30%, affecting the quality of sequencing data. What causes excessive debris, and how to reduce the debris rate?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Excessive debris is caused by the fragile cell membrane of spleen immune cells, which easily rupture due to over-enzymolysis or improper centrifugation. Reduction methods: ① Shorten the enzymolysis time from 0.5-2 hours to 0.5-1 hour to avoid over-enzymolysis; ② Set moderate acceleration and deceleration during centrifugation (avoiding excessive speed) to reduce liquid impact on cells; ③ Use low-adhesion pipette tips for cell resuspension and pipette gently (avoiding violence). This can reduce the cell debris rate from 30% to below 15%, meeting the requirements of single-cell sequencing.\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"2 reactions\/kit","offer_id":47833516179668,"sku":"FG-BA3324-2rxns","price":69.0,"currency_code":"USD","in_stock":false},{"title":"10 reactions\/kit","offer_id":46299524366548,"sku":"FG-BA3324-10rxns","price":269.0,"currency_code":"USD","in_stock":true},{"title":"50 reactions\/kit","offer_id":47707500511444,"sku":"FG-BA3324-50rxns","price":569.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/files\/BA3324_6cb0f5fd-fd13-4950-951e-9075cce2e5c3.png?v=1774248632"},{"product_id":"lung-dissociation-kit-fg-ba3325","title":"FireGene Lung Dissociation Kit - scRNA-seq Compatible","description":"\u003ch3 id=\"overview\"\u003eOverview\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Lung Dissociation Kit\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eis specially formulated to isolate high-viability single cells from lung tissue using a powerful enzymatic protocol. Ideal for research in pulmonary diseases and immune profiling, this kit provides clean, consistent cell suspensions for advanced applications such as scRNA-seq and cytometry.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch3 id=\"background-information\"\u003eBackground Information\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eDriven by Clinical and Scientific Research Needs:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eResearch into lung diseases like\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eCOPD, pulmonary fibrosis\u003c\/strong\u003e, and\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003elung cancer\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003edemands single-cell resolution.\u003c\/li\u003e\n\u003cli\u003eTraditional dissociation methods fail to preserve fragile lung cell types and introduce cellular stress.\u003c\/li\u003e\n\u003cli\u003eThis kit enables:\n\u003cul\u003e\n\u003cli\u003eIdentification of alveolar, endothelial, epithelial, and immune cells within lung tissue.\u003c\/li\u003e\n\u003cli\u003eDiscovery of\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003enew biomarkers and drug targets\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003efor precision medicine.\u003c\/li\u003e\n\u003cli\u003eDeep analysis of\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ecellular responses\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eto disease and therapy.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBackground of Technological Development:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eAddresses the inefficiencies and limitations of conventional dissociation.\u003c\/li\u003e\n\u003cli\u003eEmploys:\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eOptimized enzyme combinations\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003especific to lung matrix composition.\u003c\/li\u003e\n\u003cli\u003eControlled protocols for\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003etiming, temperature, and concentration\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eEnhanced cell recovery, reduced cellular stress, and high reproducibility.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch3 id=\"detection-principle\"\u003eDetection Principle\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eUtilizes a\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003esynergistic enzymatic digestion process\u003c\/strong\u003e:\n\u003cul\u003e\n\u003cli\u003eLung tissue is\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003esectioned into small fragments\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eA stepwise reagent application is performed under\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eprecisely managed incubation conditions\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eEnzymes break down the extracellular matrix and\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eliberate single cells\u003c\/strong\u003e.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003eResult:\n\u003cul\u003e\n\u003cli\u003eA\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ehigh-quality, viable single-cell suspension\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eready for sequencing, cytometry, or cell culture workflows.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 id=\"detection-principle\"\u003eSpecifications\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eSingle-cell sequencing, cell culture or other cell-related detections\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eCompatible Sample Types\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eLung tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eSupported Instruments\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eWater bath, horizontal centrifuge, cell counter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e-20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eShelf-life\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 id=\"detection-principle\"\u003eKit Components\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e10 Reactions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 60%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003e10 Reactions\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e\u003cspan\u003eLung DS (Lung Dissociation Solution)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e2 × 1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e50 Reactions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 60%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003e50 Reactions\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e\u003cspan\u003eLung DS (Lung Dissociation Solution)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e10 × 1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eProduct FAQ\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e1.    Q: When dissociating lung tissue, it is found that the alveolar structure is difficult to destroy, and a large number of intact alveoli remain even after 1 hour of enzymolysis, resulting in extremely low single-cell yield. What is the reason, and how to adjust the operation to destroy the alveolar structure?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The failure to destroy the alveolar structure is due to insufficient mincing of lung tissue, which prevents the alveolar cavity from being exposed and the enzymolysis solution from contacting the alveolar wall fibers. Adjustment methods: ① Mince the lung tissue into pieces smaller than 0.2mm³ (1\/3 the size of regular tissue) to ensure the alveolar cavity ruptures; ② Repeatedly puncture the minced tissue with a 1mL syringe needle before enzymolysis to physically destroy the alveolar wall; ③ Add an additional 50μL of lung tissue dissociation solution during enzymolysis and extend the enzymolysis time to 1.5 hours. This can increase the alveolar structure destruction rate to over 85% and improve the single-cell yield by 60%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e2.    Q: When dissociating lung tissue from mice with chronic obstructive pulmonary disease (COPD) model, a large amount of viscous mucus appears in the cell suspension, which wraps the cells and causes filtration difficulties. How to remove this mucus?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The mucus is formed by the mixture of mucin secreted by COPD lung tissue and enzymolysis products. Removal methods: ① Add 100μL of 0.1% hyaluronidase (self-prepared, non-toxic to cells) during enzymolysis to degrade the mucopolysaccharide components in the mucus; ② Centrifuge the cell suspension at 4℃, 500×g for 3 minutes before filtration, and discard the upper mucus layer; ③ Rinse the 70μm cell sieve 3 times with PBS containing 2% FBS to ensure the mucus flows out with the filtrate and avoid sieve clogging. This can increase the filtration speed by 50%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e3.    Q: When using a hybridization oven for enzymolysis, the liquid in the centrifuge tube is found to be layered (clear upper layer and turbid lower layer), resulting in uneven enzymolysis efficiency. What is the reason, and how to solve the liquid stratification problem?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Stratification occurs because lung tissue contains high-density blood vessels and fiber components, which settle to the bottom of the tube when the rotation speed of the hybridization oven is insufficient, leading to uneven contact between the enzymolysis solution and the tissue. Solutions: ① Increase the rotation speed of the hybridization oven from 20-30 rpm to 25-35 rpm to enhance liquid convection; ② Add 1 sterile magnetic stir bar (3mm in diameter, self-prepared) to the centrifuge tube before enzymolysis to assist mixing by magnetic force; ③ Manually invert the centrifuge tube once every 15 minutes to ensure full contact between the tissue and the enzymolysis solution. This can improve the enzymolysis uniformity by 70%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e4.    Q: After opening the kit, the lung tissue dissociation solution is stored at -20℃ for 5 months. When used, it is found that the tissue still remains in blocks after enzymolysis, and the enzyme activity decreases significantly. How to quickly determine if the dissociation solution is completely invalid?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: It can be judged by a \"mini enzymolysis experiment\": ① Mix 50μL of the dissociation solution with 10mg of minced lung tissue and incubate at 37℃ for 30 minutes; ② Pipette 10 times. If the tissue pieces are still larger than 1mm³ and no obvious cells are released, the dissociation solution is completely invalid; ③ If a small number of cells are released but enzymolysis is insufficient, double the amount of dissociation solution to 480μL and extend the enzymolysis time to 2.5 hours. This can barely meet the needs of basic experiments (not recommended for single-cell sequencing).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e5.    Q: When dissociating lung tissue from newborn mice (within 3 days of birth), a large number of cells tend to die (viability is only 40%). What causes this, and how to adjust the operation to protect the immature lung cells?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The cell membrane of immature lung cells is fragile and easily damaged by the enzymolysis solution and mechanical force. Adjustment methods: ① Shorten the enzymolysis time from 0.5-2 hours to 20-30 minutes to reduce cell damage caused by enzymes; ② Use a water bath for enzymolysis and gently shake every 5 minutes (avoid violent oscillation); ③ Replace the regular PBS containing 5% FBS with PBS containing 10% FBS for washing to enhance cell protection. This can increase the cell viability to over 75%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e6.    Q: After centrifugation, a large number of black particles (suspected to be carbon dust) are found mixed in the cell pellet, which affects subsequent cell counting and staining. How to remove these impurity particles?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The black particles are mostly foreign impurities inhaled by lung tissue or formed by the aggregation of cell debris. Removal methods: ① After centrifugation, gently pipette the pellet with 1mL of PBS containing 5% FBS to suspend the particles; ② Let it stand for 2 minutes. After the particles settle, aspirate the upper cell suspension and transfer it to a new centrifuge tube; ③ Repeat the operation twice. This can remove over 80% of the black particles without affecting cell viability (viability loss ≤5%).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e7.    Q: When using a water bath for enzymolysis, observation is forgotten due to experimental interruption, and the enzymolysis time lasts for 3 hours. It is found that the cell viability drops to 30%. How to remedy to retain some usable cells?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: A large number of cells die due to over-enzymolysis, and viable cells can be screened by \"gradient centrifugation\": ① Centrifuge the cell suspension at 4℃, 200×g for 3 minutes, and discard the upper layer of dead cells and debris; ② Resuspend the pellet with 5mL of PBS containing 10% FBS and centrifuge at 4℃, 300×g for 5 minutes; ③ Take the pellet and stain with trypan blue. Viable cells are mostly concentrated in the lower layer of the pellet. This can screen out cells with viability ≥60% (yield is about 30% of the original), which is only suitable for experiments with low cell quantity requirements such as cell culture.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e8.    Q: After dissociation, red blood cells account for 55% of the cell suspension. When treated with \u003ca href=\"https:\/\/firegene.com\/products\/red-blood-cell-lysis-kit-fg-ba3311?_pos=6\u0026amp;_sid=968f93520\u0026amp;_ss=r\"\u003eFG-BA3311\u003c\/a\u003e Red Blood Cell Lysis Buffer, the viability of pulmonary vascular endothelial cells drops from 80% to 50%. How to optimize the lysis conditions to reduce damage to endothelial cells?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The damage to endothelial cells is due to the certain toxicity of the lysis buffer to vascular endothelial cells. Optimization methods: ① Dilute the lysis buffer 1-fold with PBS to reduce its concentration; ② Control the incubation temperature at 4℃ and shorten the time to 2-3 minutes. Terminate the reaction immediately after observing red blood cell lysis under a microscope; ③ Wash 3 times with PBS containing 10% FBS after lysis to fully remove the residual lysis buffer. This can maintain the viability of pulmonary vascular endothelial cells at over 70%.\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"2 reactions\/kit","offer_id":47833516245204,"sku":"FG-BA3325-2rxns","price":99.0,"currency_code":"USD","in_stock":false},{"title":"10 reactions\/kit","offer_id":46299524399316,"sku":"FG-BA3325-10rxns","price":329.0,"currency_code":"USD","in_stock":true},{"title":"50 reactions\/kit","offer_id":47707826815188,"sku":"FG-BA3325-50rxns","price":709.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/files\/BA3325.png?v=1774251144"},{"product_id":"human-brain-dissociation-kit-fg-ba3326","title":"FireGene Human Brain Dissociation Kit for Neuroscience Research","description":"\u003ch3 id=\"overview\"\u003eOverview\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Human Brain Dissociation Kit\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eis a high-efficiency enzymatic system designed to dissociate human brain tissue into high-viability single-cell suspensions. Ideal for neurological research and single-cell sequencing, this kit enables accurate profiling of brain cell populations involved in health and disease.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch3 id=\"background-information\"\u003eBackground Information\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eDriven by Clinical and Scientific Research Needs:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSingle-cell sequencing of human brain tissue is essential for understanding\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ecomplex neural circuits\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eand\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ebrain disorders\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eTraditional dissociation methods struggle with preserving diverse brain cell types such as neurons and glia.\u003c\/li\u003e\n\u003cli\u003eThis kit enables:\n\u003cul\u003e\n\u003cli\u003eHigh-resolution analysis of\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ecellular heterogeneity\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003ein both healthy and diseased brain tissues.\u003c\/li\u003e\n\u003cli\u003eIdentification of\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ebiomarkers and therapeutic targets\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003efor conditions like\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eneurodegeneration, epilepsy\u003c\/strong\u003e, and\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003emental health disorders\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eAdvancement of\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eprecision neurology\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003ethrough better cellular insight.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBackground of Technological Development:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eOlder dissociation methods often lead to low cell yields and viability.\u003c\/li\u003e\n\u003cli\u003eFireGene’s solution:\n\u003cul\u003e\n\u003cli\u003eUses\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ea synergistic enzymatic blend\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eoptimized for fragile neural tissue.\u003c\/li\u003e\n\u003cli\u003eFeatures carefully calibrated\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eenzyme types, concentrations, and temperature conditions\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eMaximizes viable cell recovery for\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ereliable, reproducible results\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003ein downstream assays.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch3 id=\"detection-principle\"\u003eDetection Principle\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eUtilizes a\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003estepwise enzymatic digestion method\u003c\/strong\u003e:\n\u003cul\u003e\n\u003cli\u003eBrain samples are\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ecut into small pieces\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eto enhance enzyme penetration.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEnzymes are added sequentially\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eunder optimal incubation conditions.\u003c\/li\u003e\n\u003cli\u003eExtracellular matrix and intercellular junctions are enzymatically broken down.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003eResult:\n\u003cul\u003e\n\u003cli\u003eA\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eclean, viable single-cell suspension\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003esuitable for scRNA-seq, brain atlas mapping, flow cytometry, or culture.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 id=\"detection-principle\"\u003eSpecifications\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eSingle-cell sequencing, cell culture or other cell-related detections\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eCompatible Sample Types\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eHuman brain tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eSupported Instruments\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eWater bath, horizontal centrifuge, cell counter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e-20 °C \/ 4 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eShelf-life\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e24 months at -20 °C\u003cbr\u003e12 months at 4 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 id=\"detection-principle\"\u003eKit Components\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e10 Reactions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"width: 25%; padding: 8px; border: 1px solid #ddd; background: #f0f0f0;\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"width: 25%; padding: 8px; border: 1px solid #ddd; background: #f0f0f0;\"\u003e10 Reactions\/Kit\u003c\/th\u003e\n\u003cth style=\"width: 25%; padding: 8px; border: 1px solid #ddd; background: #f0f0f0;\"\u003eStorage\u003c\/th\u003e\n\u003cth style=\"width: 25%; padding: 8px; border: 1px solid #ddd; background: #f0f0f0;\"\u003eShelf-life\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003eBuffer A\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e1*21.6 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e-20 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003eEnzyme B\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e1*8 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e-20 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003eEnzyme C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e1*100 μL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e4 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e\u003cspan\u003eDRS (Human Brain Cell Debris Removal Buffer)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e1*10 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e4 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e50 Reactions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"width: 25.0447%; padding: 8px; border: 1px solid rgb(221, 221, 221); background: rgb(240, 240, 240);\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"width: 25.0447%; padding: 8px; border: 1px solid rgb(221, 221, 221); background: rgb(240, 240, 240);\"\u003e50 Reactions\/Kit\u003c\/th\u003e\n\u003cth style=\"width: 25.0447%; padding: 8px; border: 1px solid rgb(221, 221, 221); background: rgb(240, 240, 240);\"\u003eStorage\u003c\/th\u003e\n\u003cth style=\"width: 25.0447%; padding: 8px; border: 1px solid rgb(221, 221, 221); background: rgb(240, 240, 240);\"\u003eShelf-life\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 25.0447%;\"\u003eBuffer A\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 25.0447%;\"\u003e5*21.6 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 25.0447%;\"\u003e-20 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 25.0447%;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 25.0447%;\"\u003eEnzyme B\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 25.0447%;\"\u003e5*8 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 25.0447%;\"\u003e-20 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 25.0447%;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 25.0447%;\"\u003eEnzyme C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 25.0447%;\"\u003e5*100 μL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 25.0447%;\"\u003e4 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 25.0447%;\"\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 25.0447%;\"\u003e\u003cspan\u003eDRS (Human Brain Cell Debris Removal Buffer)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 25.0447%;\"\u003e5*10 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 25.0447%;\"\u003e4 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 25.0447%;\"\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eProduct FAQ\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e1.    Q: When dissociating human brain tissue, flocculent precipitates appear immediately after adding Enzyme C (10μL\/reaction). Will this affect the enzymolysis effect? How to handle the precipitation issue?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Flocculent precipitates are mostly caused by a temporary reaction between Enzyme C (enzyme preparation) and ionic components in Buffer A. If the precipitates dissolve on their own within 10 minutes, the enzymolysis effect will not be affected. If the precipitates persist, reconfigure the reaction system: ① First, mix 10μL Enzyme C with 800μL Enzyme B uniformly to form an \"enzyme mixture\"; ② Slowly add the mixture dropwise into Buffer A containing the tissue, and invert to mix while adding. This avoids precipitation caused by local high concentration and ensures stable enzymolysis efficiency.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e2.    Q: After adding DRS (Debris Removal Solution) in Step 11, the cell suspension is pipetted 10 times. What impact will excessive pipetting force or insufficient pipetting times have on cell separation? How to define the standard for \"gentle pipetting\"?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Excessive pipetting force: Damages the cell membrane of fragile cells such as neurons and glial cells, reducing cell viability by 30%-40%, with a large number of cell debris visible under the microscope. Insufficient pipetting times (\u0026lt;8 times): DRS cannot fully contact the cell suspension, so debris cannot float up effectively during subsequent centrifugation and stratification, reducing cell purity by 50%. Standard for \"gentle pipetting\": Use a 5mL pipette, insert the pipette tip slowly 1cm below the liquid surface when aspirating, control the aspiration time to 2 seconds, and push the liquid out slowly along the tube wall when dispensing. Avoid generating air bubbles and ensure stable, non-impact liquid flow.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e3.    Q: The centrifugation parameters in Step 13 are \"4℃, 3000×g, 20 minutes, medium acceleration\/deceleration\". If the laboratory's horizontal centrifuge does not have the \"medium acceleration\/deceleration\" function and only has \"fast\" or \"slow\" options, how to adjust the operation?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: If medium acceleration\/deceleration is unavailable, prioritize \"slow acceleration\/deceleration\" (acceleration time ≥5 minutes, deceleration time ≥8 minutes) and extend the centrifugation time to 25 minutes: ① Slow acceleration prevents liquid disturbance due to sudden increase in centrifugal force, ensuring stable stratification of DRS and cells; ② Slow deceleration prevents bottom cells from being scattered by inertia, reducing mixing with the debris layer. If only fast acceleration\/deceleration is available, let the centrifuge tube stand for 2-5 minutes after centrifugation before taking it out to stabilize the stratification interface. This reduces the probability of cell-debris mixing by 30%, but the cell yield will still be approximately 15% lower than that with medium acceleration\/deceleration.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e4.    Q: When dissociating aged human brain tissue (refrigerated for more than 6 hours after sampling), the cell viability is only 30%, much lower than the 70% of fresh tissue. How to optimize the operation to improve the cell viability of aged tissue?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The low viability of aged tissue requires optimization from two aspects: \"reducing cell damage\" and \"enhancing cell protection\": ① Immediately soak the tissue in PBS containing 10% FBS (5% for regular use) after sampling, and transport it under refrigeration at 4℃ to avoid tissue dehydration; ② Reduce the amount of Enzyme B to 600μL (800μL for regular use) during enzymolysis, and shorten the enzymolysis time to 15-20 minutes to reduce enzyme-induced damage to fragile cells; ③ Replace regular PBS with PBS containing 1% BSA in the washing step to enhance cell membrane protection. These measures can increase the cell viability of aged tissue to over 50%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e5.    Q: After adding red blood cell lysis buffer (\u003ca name=\"OLE_LINK2\"\u003e\u003c\/a\u003e\u003ca href=\"https:\/\/firegene.com\/products\/red-blood-cell-lysis-kit-fg-ba3311?_pos=5\u0026amp;_sid=8111c3f2d\u0026amp;_ss=r\"\u003e\u003cu\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Times New Roman;\"\u003eFG-\u003c\/span\u003e\u003c\/span\u003e\u003c\/u\u003e\u003cu\u003e\u003cspan class=\"15\"\u003eBA3311\u003c\/span\u003e\u003c\/u\u003e\u003c\/a\u003e) in Step 15, the mixture is incubated on ice for 5 minutes. What impact will prolonged incubation (e.g., 10 minutes) or insufficient incubation (e.g., 2 minutes) have on human brain cells (e.g., neurons)?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Prolonged incubation: Red blood cell lysis buffer has mild toxicity to neurons; 10-minute incubation reduces neuron viability by 25%-30%, and glial cells tend to shrink. Insufficient incubation: Red blood cells are not fully lysed, with residual red blood cells accounting for over 20%, which interferes with cell capture in subsequent single-cell sequencing (non-specific adsorption to the sequencing chip). The optimal operation is to observe under a microscope every 2 minutes and terminate the incubation immediately when red blood cells become transparent (approximately 4-5 minutes). This balances the effect of red blood cell removal and cell viability protection.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e6.    Q: Step 20 mentions \"if obvious cell clumping occurs, filter with a 20μm cell sieve\". However, after filtration, a large number of neurons (with a diameter of approximately 8-12μm) are retained. What is the reason, and how to adjust the filtration operation?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Neurons are retained mostly because they form clumps \u0026gt;20μm by entangling with glial cells and debris, rather than single neurons being retained. Adjustment methods: ① Before filtration, gently pipette the cell suspension 15 times with a 1mL low-adhesion pipette tip (avoid violence) to disperse small clumps \u0026lt;20μm; ② Pre-wet the 20μm cell sieve with PBS containing 5% FBS to reduce cell adsorption to the sieve; ③ Gently push the liquid with a 5mL syringe during filtration to avoid clump compression caused by sieve clogging. These measures can reduce the neuron retention rate from 40% to below 10%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e7.    Q: After opening Buffer A in the kit, it is stored at 4℃ for 1 month. When used, slight turbidity is found in the solution. Is it still usable? How to quickly determine if the turbid Buffer A is invalid?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Slight turbidity may be caused by the low-temperature precipitation of components in Buffer A. Its validity needs to be verified first: ① Take 100μL of turbid Buffer A and heat it in a 37℃ water bath for 10 minutes. If the turbidity disappears, it indicates normal precipitation; the buffer can be used after shaking uniformly. ② If the turbidity persists after heating or flocculent precipitates appear, Buffer A is contaminated or deteriorated and cannot be used. To avoid turbidity after opening, aliquot Buffer A into 2mL\/tube (one tube for one experiment) after opening, store it frozen at -20℃, and thaw and mix well before each use. This extends the validity period to 3 months.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e8.    Q: A 70μm cell sieve is used for filtration in Steps 5 and 6. If the sieve is severely clogged, the filtration speed is extremely slow, and a large amount of white flocculent material (suspected to be nerve fibers) remains on the sieve, how to pretreat to reduce nerve fiber residue?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The white flocculent material is nerve fibers. A \"pre-digestion\" step needs to be added before enzymolysis: ① After mincing the tissue, soak it in 2160μL Buffer A for 10 minutes, and gently shake every 5 minutes; ② Add 50μL Enzyme B (1\/16 of the regular 800μL), incubate at 37℃ for 5 minutes to preliminarily degrade nerve fibers; ③ Add the remaining Enzyme B and Enzyme C according to the regular procedure. This reduces nerve fiber formation by 60%, avoids sieve clogging, and increases the filtration speed by 40%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e9.    Q: After quality control, it is found that glial cells account for 90% of the cell suspension, while neurons only account for 10%—much lower than the normal neuron proportion in brain tissue (approximately 30%). What causes neuron loss, and how to optimize the collection method?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Neuron loss is mostly due to \"weak neuron sedimentation capacity\" in centrifugation and filtration steps: ① In Step 7, increase the centrifugation speed from 300×g to 350×g and extend the centrifugation time to 8 minutes to enhance neuron precipitation; ② Before filtration in Step 20, if there is no obvious cell clumping, skip the 20μm cell sieve filtration (neurons are easily adsorbed by the sieve); ③ When aspirating the supernatant in Step 14, retain 100μL of supernatant to mix with the cell pellet to avoid the loss of small-volume neurons with the supernatant. These measures can increase the neuron proportion to approximately 25%.\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"2 reactions\/kit","offer_id":47833516474580,"sku":"FG-BA3326-2rxns","price":109.0,"currency_code":"USD","in_stock":false},{"title":"10 reactions\/kit","offer_id":46299524432084,"sku":"FG-BA3326-10rxns","price":429.0,"currency_code":"USD","in_stock":true},{"title":"50 reactions\/kit","offer_id":47708600697044,"sku":"FG-BA3326-50rxns","price":1419.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/files\/BA3326.png?v=1774256387"},{"product_id":"trachea-dissociation-kit-fg-ba3328","title":"FireGene Trachea Dissociation Kit - Respiratory Cell Isolation","description":"\u003ch3 id=\"overview\"\u003eOverview\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Trachea Dissociation Kit\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eis a specialized enzymatic solution for isolating high-quality single-cell suspensions from tracheal tissue. Designed to support respiratory research and single-cell applications, this kit offers exceptional cell recovery and viability, ideal for understanding airway biology and disease mechanisms.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch3 id=\"background-information\"\u003eBackground Information\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eDriven by Clinical and Scientific Research Needs:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eStudying respiratory diseases like\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003etracheitis\u003c\/strong\u003e,\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003etracheal tumors\u003c\/strong\u003e, and\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eairway inflammation\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003erequires understanding cell-level heterogeneity.\u003c\/li\u003e\n\u003cli\u003eTraditional dissociation approaches often compromise rare or delicate cell types.\u003c\/li\u003e\n\u003cli\u003eThis kit enables:\n\u003cul\u003e\n\u003cli\u003eIdentification of\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eepithelial cells, ciliated cells, immune cells\u003c\/strong\u003e, and more from tracheal samples.\u003c\/li\u003e\n\u003cli\u003eDiscovery of\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ebiomarkers\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eand\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003epathways related to inflammation and remodeling\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eSupport for targeted therapy development and\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eprecision medicine\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003ein respiratory health.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBackground of Technological Development:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eMechanical and chemical methods lack consistency and damage viable cells.\u003c\/li\u003e\n\u003cli\u003eFireGene’s approach integrates:\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eOptimized enzymatic digestion\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003etailored to tracheal matrix structure.\u003c\/li\u003e\n\u003cli\u003eAdjusted protocols for\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ereaction time, temperature, and reagent ratios\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eSuperior\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ecell viability, efficiency, and reproducibility\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003ein tracheal tissue dissociation workflows.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch3 id=\"detection-principle\"\u003eDetection Principle\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eEmploys a\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003emulti-enzyme digestion strategy\u003c\/strong\u003e:\n\u003cul\u003e\n\u003cli\u003eTrachea tissue is\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eminced into small fragments\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eA controlled sequence of\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eenzyme application\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eis used.\u003c\/li\u003e\n\u003cli\u003eEnzymes gradually break down\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eextracellular matrix and cell-cell junctions\u003c\/strong\u003e.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003eResult:\n\u003cul\u003e\n\u003cli\u003eA\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eclean, viable single-cell suspension\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eoptimized for scRNA-seq, immune profiling, and respiratory disease modeling.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 id=\"detection-principle\"\u003eSpecifications\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eSingle-cell sequencing, cell culture or other cell-related detections\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eCompatible Sample Types\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eTrachea tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eSupported Instruments\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eWater bath, horizontal centrifuge, cell counter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e-20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eShelf-life\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 id=\"detection-principle\"\u003eKit Components\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e10 Reactions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 60%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003e10 Reactions\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e\u003cspan\u003eTrachea DS 1 (Trachea Dissociation Solution)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e\u003cspan\u003e1*30 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221);\"\u003e\u003cspan\u003eTrachea DS 2 (Trachea Dissociation Solution)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221);\"\u003e\u003cspan\u003e1*30 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e50 Reactions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40.0716%; font-weight: bold; padding: 8px; border: 1px solid rgb(221, 221, 221);\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 59.9284%; font-weight: bold; padding: 8px; border: 1px solid rgb(221, 221, 221);\"\u003e50 Reactions\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 40.0716%;\"\u003e\u003cspan\u003eTrachea DS 1 (Trachea Dissociation Solution)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 59.9284%;\"\u003e\u003cspan\u003e5*30 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 40.0716%;\"\u003e\u003cspan\u003eTrachea DS 2 (Trachea Dissociation Solution)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 59.9284%;\"\u003e\n\u003cp\u003e\u003cspan\u003e5*30 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eProduct FAQ\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e1.    Q: When dissociating tracheal tissue, according to the procedure, first digest with Dissociation Buffer 1 for 15-30 minutes. After centrifugation, it is found that the tissue blocks are still large and cannot be fully resuspended with Dissociation Buffer 2. What is the reason, and how to handle the insufficiently digested tissue blocks?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The large size of tissue blocks is due to the failure of Dissociation Buffer 1 to fully degrade the cartilage and fibrous tissue in the outer layer of the trachea. Handling methods: ① Re-mince the centrifuged tissue blocks to less than 0.2mm³ (half the size of the first mincing), rinse repeatedly with 1mL PBS to remove residual Dissociation Buffer 1; ② Add 2mL of Dissociation Buffer 1 supplementally, incubate at 37℃ for 10 minutes, and shake gently every 3 minutes to enhance enzymolysis effect; ③ After centrifugation again, the tissue blocks can be reduced to an appropriate size and evenly dispersed when resuspended with Dissociation Buffer 2, ensuring sufficient subsequent enzymolysis.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e2.    Q: When dissociating tracheal tissue from asthmatic model mice, a large amount of viscous secretions appear in the cell suspension, which wrap the cells and cause filtration difficulties. How to remove these secretions?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The viscous secretions are formed by the mixture of mucin secreted by asthmatic tracheal tissue and enzymolysis products. Removal methods: ① Add 100μL of 0.1% hyaluronidase (self-prepared, non-toxic to cells) during digestion with Dissociation Buffer 2 to degrade the mucopolysaccharide components in the secretions; ② Centrifuge the cell suspension at 4℃, 500×g for 3 minutes before filtration, and discard the upper viscous layer; ③ Rinse the 70μm cell sieve 3 times with PBS containing 2% FBS to ensure the secretions flow out with the filtrate and avoid sieve clogging, which can increase the filtration speed by 50%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e3.    Q: When using a hybridization oven for enzymolysis of Dissociation Buffer 2 at a rotation speed of 20-30 rpm, it is found that the liquid in the centrifuge tube forms a vortex, causing tissue blocks to aggregate at the bottom of the tube and fail to be enzymolyzed. What is the reason, and how to avoid vortex formation?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The vortex is formed because the tracheal tissue contains high-density cartilage debris, which drives the liquid to rotate when the rotation speed of the hybridization oven is too high. Avoidance methods: ① Reduce the rotation speed of the hybridization oven to 15-20 rpm to reduce the liquid rotation force; ② Put 1 sterile glass bead (2mm in diameter, self-prepared) into the centrifuge tube, and use the collision of the glass bead to disperse the tissue blocks; ③ Manually invert the centrifuge tube once every 20 minutes to ensure full contact between the tissue blocks and Dissociation Buffer 2, which can improve the enzymolysis uniformity by 70%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e4.    Q: After opening Dissociation Buffer 1 and Dissociation Buffer 2 in the kit, they are stored at -20℃ for 5 months. When used, it is found that the enzymolysis efficiency of Dissociation Buffer 2 decreases (the cell yield is only 55% of the original). Is the reagent invalid, and how to judge and remedy it?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The validity period of the opened dissociation buffers stored at -20℃ is 3 months (2 years for unopened ones), and 5 months of storage may lead to decreased enzyme activity. Judgment methods: ① Mix 50μL of Dissociation Buffer 2 with a small amount of minced tracheal tissue, incubate at 37℃ for 30 minutes. If there is no obvious change in the tissue blocks, it indicates insufficient enzyme activity; ② Remedial methods: Increase the dosage of Dissociation Buffer 2 by 50%, extend the digestion time to 2 hours, and add 50μL of 1% BSA (self-prepared) to Dissociation Buffer 2 at the same time to enhance enzyme stability. This can restore the cell yield to about 80% of the original (not recommended for single-cell sequencing).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e5.    Q: In Step 10, rinse the digestion tube with 6mL of RPMI 1640 medium. If a large number of epithelial cells (target tracheal cells) are still attached to the tube wall after rinsing, resulting in low cell yield, how to optimize the rinsing operation?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The adhesion of epithelial cells is due to the adhesion molecules contained in tracheal epithelial cells, which are easy to attach to the inner wall of the centrifuge tube. Optimization methods: ① Rinse the inner wall of the centrifuge tube with PBS containing 5% FBS before rinsing to form a protective film and reduce cell adhesion; ② Divide 6mL of medium into 3 times for rinsing (2mL each time), and invert the centrifuge tube repeatedly 10 times each time to ensure every area of the tube wall is rinsed; ③ Gently scrape the tube wall with a 1mL pipette tip after rinsing, and then rinse with a small amount of medium, which can increase the recovery rate of epithelial cells by 40%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e6.    Q: When dissociating tracheal tissue from newborn mice (within 5 days of birth), the cell viability is only 30%, which is much lower than 75% of adult mice. What is the reason, and how to adjust the operation to protect immature tracheal cells?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The cell membrane of immature tracheal cells is fragile and easily damaged by enzymolysis solution and mechanical force. Adjustment methods: ① Shorten the digestion time of Dissociation Buffer 1 from 15-30 minutes to 10-15 minutes, and the digestion time of Dissociation Buffer 2 to 30-40 minutes to reduce enzyme damage; ② Use a water bath for enzymolysis, and shake gently every 5 minutes (avoid violent oscillation) to reduce mechanical impact; ③ In the washing step, replace the conventional PBS containing 5% FBS with PBS containing 10% FBS to enhance cell membrane protection, which can increase the viability of immature tracheal cells to more than 65%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e7.    Q: After centrifugation in Steps 5 and 6, it is required to \"do not aspirate the tissue\". If a small amount of tissue blocks are accidentally aspirated, what impact will it have on the subsequent experiment, and how to recover the aspirated tissue blocks?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Aspirating tissue blocks will lead to the loss of target cells, reducing the cell yield by 20%-30%. Recovery methods: ① If the tissue blocks are still in the pipette tip, immediately squeeze the liquid in the tip back into the centrifuge tube; ② If the supernatant has been aspirated, collect the supernatant into a new centrifuge tube, centrifuge at 4℃, 300×g for 8 minutes, discard the supernatant, and combine the precipitate with the original tissue blocks; ③ Add 0.5mL of Dissociation Buffer 2 supplementally during subsequent enzymolysis, and extend the digestion time by 10 minutes to make up for cell loss, which can restore the cell yield to 85% of the normal level.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e8.    Q: A large number of transparent cartilage debris appear in the cell suspension after dissociation, interfering with cell counting. How to remove these cartilage debris?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Cartilage debris is a product of incomplete enzymolysis of the cartilage tissue in the outer layer of the trachea. Removal methods: ① Before filtration after enzymolysis, let the cell suspension stand for 5 minutes, the cartilage debris will settle to the bottom of the tube, and aspirate the upper cell suspension for filtration first; ② When filtering with a 70μm cell sieve, rinse the sieve repeatedly with PBS containing 2% FBS, the cartilage debris will remain on the sieve, and gently scrape it off with tweezers; ③ If there is still residue, perform secondary filtration with a 40μm cell sieve (only retain the filtrate), which can remove more than 90% of the cartilage debris without affecting the recovery rate of epithelial cells.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e9.    Q: When using \u003ca href=\"https:\/\/firegene.com\/products\/red-blood-cell-lysis-kit-fg-ba3311?_pos=5\u0026amp;_sid=8111c3f2d\u0026amp;_ss=r\"\u003e\u003cu\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Times New Roman;\"\u003eFG-\u003c\/span\u003e\u003c\/span\u003e\u003c\/u\u003e\u003cu\u003e\u003cspan class=\"15\"\u003eBA3311\u003c\/span\u003e\u003c\/u\u003e\u003c\/a\u003e Red Blood Cell Lysis Buffer to remove red blood cells in Step 15, it is found that the viability of tracheal epithelial cells drops from 80% to 50%. How to optimize the red blood cell lysis operation to reduce damage to epithelial cells?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: The damage to epithelial cells is due to the slight toxicity of the lysis buffer to tracheal epithelial cells. Optimization methods: ① Dilute the lysis buffer 1-fold with PBS to reduce its concentration; ② Control the incubation temperature at 4℃, shorten the time to 3-4 minutes, and terminate immediately when red blood cell lysis is observed under a microscope; ③ Wash 3 times with PBS containing 10% FBS after lysis (1 time for conventional operation) to fully remove residual lysis buffer, which can maintain the viability of tracheal epithelial cells above 70%.\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"2 reactions\/kit","offer_id":47833608585428,"sku":"FG-BA3328-2rxns","price":69.0,"currency_code":"USD","in_stock":true},{"title":"10 reactions\/kit","offer_id":46299524464852,"sku":"FG-BA3328-10rxns","price":289.0,"currency_code":"USD","in_stock":true},{"title":"50 reactions\/kit","offer_id":47709125771476,"sku":"FG-BA3328-50rxns","price":889.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/files\/BA3328.png?v=1774258839"},{"product_id":"pancreas-dissociation-kit-fg-ba3314","title":"FireGene Pancreas Dissociation Kit - High-Fidelity Enzymatic Digestion","description":"\u003ch3 id=\"overview\"\u003eOverview\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Pancreas Dissociation Kit\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eis designed for the efficient enzymatic dissociation of pancreatic tissue into viable single-cell suspensions. Ideal for single-cell sequencing and disease modeling, this kit supports investigations into diabetes, pancreatitis, and pancreatic cancer with high precision and reproducibility.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch3 id=\"background-information\"\u003eBackground Information\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eDriven by Clinical and Scientific Research Needs:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eUnderstanding pancreatic disease mechanisms, such as\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ediabetes, chronic inflammation\u003c\/strong\u003e, and\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003epancreatic cancer\u003c\/strong\u003e, requires detailed single-cell analysis.\u003c\/li\u003e\n\u003cli\u003eTraditional methods do not fully preserve\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eislet cells\u003c\/strong\u003e,\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eacinar cells\u003c\/strong\u003e, and other fragile populations.\u003c\/li\u003e\n\u003cli\u003eThis kit enables:\n\u003cul\u003e\n\u003cli\u003eIsolation and profiling of pancreatic cell types for\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ebiomarker discovery\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eImproved insight into\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eimmune response, fibrosis, and beta-cell function\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eDevelopment of\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003etargeted therapies\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eand regenerative strategies for pancreatic diseases.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBackground of Technological Development:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eFireGene overcomes the limitations of low cell yield and viability by using:\n\u003cul\u003e\n\u003cli\u003eA\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eproprietary enzymatic formulation\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003edesigned specifically for pancreatic ECM.\u003c\/li\u003e\n\u003cli\u003eOptimized conditions for\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eincubation time, temperature, and enzyme concentration\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eA workflow that preserves\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ecell integrity\u003c\/strong\u003e, ensuring consistent and high-quality single-cell suspensions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch3 id=\"detection-principle\"\u003eDetection Principle\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eBased on a\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003esynergistic enzymatic digestion process\u003c\/strong\u003e:\n\u003cul\u003e\n\u003cli\u003ePancreatic tissue is\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eminced and treated sequentially\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003ewith prepared reagents.\u003c\/li\u003e\n\u003cli\u003eThe digestion proceeds under\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003econtrolled conditions\u003c\/strong\u003e, breaking down ECM components and intercellular junctions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003eFinal Result:\n\u003cul\u003e\n\u003cli\u003eA\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eclean, high-viability single-cell suspension\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eideal for use in scRNA-seq, proteomics, and pancreatic disease research.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 id=\"detection-principle\"\u003eSpecifications\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eSingle-cell sequencing, cell culture or other cell-related detections\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eCompatible Sample Types\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003ePancreas tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eSupported Instruments\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eWater bath, horizontal centrifuge, cell counter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e-80 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eShelf-life\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 id=\"detection-principle\"\u003eKit Components\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40.0716%; font-weight: bold; padding: 8px; border: 1px solid rgb(221, 221, 221);\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 59.9284%; font-weight: bold; padding: 8px; border: 1px solid rgb(221, 221, 221);\"\u003e8 Tests\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 40.0716%;\"\u003e\n\u003cp\u003ePancreas Dissociation Kit\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); width: 59.9284%;\"\u003e8 × 3 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eProduct FAQ\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003e1.   Q: Is this kit only suitable for mammalian pancreatic tissue? Are there differences in dissociation effects on different parts of the pancreas (e.g., islets, exocrine portion)? Can it be used for pancreatic tumor tissue or non-mammalian pancreatic tissue (e.g., avian pancreas)?\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The kit is only suitable for normal pancreatic tissue and pancreatic cancer tissue, and is not temporarily recommended for non-mammalian pancreatic tissue. The cell membrane characteristics of non-mammalian pancreatic tissue (such as avian pancreas) are significantly different from those of mammals, making it difficult to obtain intact single cells after dissociation. There are slight differences in dissociation effects on different parts of the pancreas: For islet tissue (containing sensitive endocrine cells), the digestion time needs to be shortened (it is recommended to complete quality inspection within 0.5 hours) to avoid reduced viability due to excessive enzymolysis; the exocrine portion has slightly stronger tolerance and can be digested for the conventional 0.5 hours. However, both can eventually obtain high-viability single cells with no significant difference in yield.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e2.    Q: The kit is labeled \"8 rxns\", and the dosage of pancreatic tissue dissociation solution per experiment is 3mL. If only 100mg of pancreatic tissue (1\/2 of the standard dosage) is processed in a single experiment, can the dosage of dissociation solution be proportionally reduced to 1.5mL?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Proportional reduction to 1.5mL is not recommended. The enzyme concentration and buffer system in the pancreatic tissue dissociation solution are designed for 3mL volume to match 200mg of tissue. If reduced to 1.5mL, the contact area between the enzyme and the tissue will be significantly reduced, and the excessively small liquid volume may lead to high local enzyme concentration. On the contrary, this will cause over-digestion of some tissues and incomplete dissociation of others. When processing 100mg of tissue, 3mL of dissociation solution is still required. During subsequent centrifugation to collect cells, the centrifugation time can be extended (from 5 minutes to 8 minutes) to ensure sufficient cell precipitation and avoid cell loss due to the relatively large liquid volume.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e3.    Q: During digestion in Step 3, a water bath requires \"manual shaking every 3-5 minutes\", and a hybridization oven requires \"a rotation speed of 20-30 rpm\". Is the only operational difference between the two devices the mixing method? Which one is more suitable for pancreatic tissue dissociation?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Beyond the mixing method, the core difference lies in temperature stability: The temperature control accuracy of the hybridization oven (±0.5℃) is higher than that of the water bath (±1℃). Moreover, the automatic rotation speed enables continuous and uniform contact between pancreatic tissue and the dissociation solution, avoiding local digestion differences caused by uneven density of pancreatic tissue. In terms of effect, the hybridization oven is more suitable for pancreatic tissue dissociation—Pancreatic tissue contains a large number of digestive enzymes. If the frequency or intensity of manual shaking is inconsistent, it is easy to cause \"local over-enzymolysis and local undigestion\"; the stable rotation speed of the hybridization oven can reduce this risk, and the single-cell yield is 15%-20% higher than that of the water bath. If a water bath is used, it is necessary to strictly shake at 3-minute intervals for 10 seconds each time to ensure no tissue settles at the bottom.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e4.    Q: Steps 5-7 require filtration with a 70μm cell sieve and rinsing the centrifuge tube 3 times to collect a total of 12mL of filtrate. What impact will omitting one rinsing step have on the experimental results? Can a 40μm or 100μm cell sieve replace the 70μm one?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Omitting one rinsing step will result in the loss of approximately 1\/3 of residual cells, reducing the final cell yield by 25%-30%. Cells after pancreatic dissociation (such as islet β cells and acinar cells) are easily adsorbed on the inner wall of the centrifuge tube, and 3 rinsing steps are the key to ensuring full cell recovery. A 40μm or 100μm cell sieve cannot be used as a replacement: The pore size of the 40μm sieve is too small and will retain some pancreatic single cells (e.g., islet cells have a diameter of about 10-15μm; although they can pass through, the sieve is easily blocked by fiber debris, leading to filtration difficulties); the pore size of the 100μm sieve is too large and cannot effectively filter the fine fiber debris remaining in pancreatic tissue. These impurities will interfere with subsequent cell counting or single-cell sequencing sample preparation.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e5.    Q: The centrifugation parameters in Steps 8 and 9 are both \"4℃, 300×g for 5 minutes\". If only a vertical centrifuge is available in the laboratory or the centrifugation speed is set incorrectly (e.g., 200×g, 400×g), what impact will this have? Can room-temperature centrifugation replace 4℃ centrifugation?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: A vertical centrifuge cannot replace a horizontal centrifuge. The direction of centrifugal force of a vertical centrifuge is perpendicular to the centrifuge tube, which will cause uneven precipitation of pancreatic cells, easily resulting in loose precipitation or adhesion to the tube wall. Cells are likely to be sucked away when discarding the supernatant; a horizontal centrifuge allows cells to precipitate evenly at the bottom of the tube, facilitating subsequent operations. Impacts of incorrect parameters: ① Rotation speed of 200×g: Cells cannot be fully precipitated and will be lost with the supernatant, reducing the yield by more than 40%; ② Rotation speed of 400×g: Excessive centrifugal force will squeeze fragile islet cells, leading to a 20%-25% decrease in viability. Room-temperature centrifugation cannot replace 4℃ centrifugation. Room temperature will accelerate the metabolism of pancreatic cells, and the residual dissociation solution has enhanced activity at room temperature, further damaging the cells. Centrifugation at 4℃ can maintain a low metabolic state of cells and reduce viability loss.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e6.    Q: The instruction manual mentions that \"DMEM medium can replace RPMI 1640 medium\". After replacement, is it necessary to adjust the number of rinsing steps or centrifugation parameters? Do the two media have an impact on the viability of pancreatic cells?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: There is no need to adjust the number of rinsing steps or centrifugation parameters after replacement. Both DMEM and RPMI 1640 are common basal media for mammalian cells. Although they differ in amino acid and vitamin content, both can provide a suitable osmotic pressure and pH environment for pancreatic cells, with no impact on dissociation efficiency. The impact on the viability of pancreatic cells is minimal: Experimental data shows that the difference in pancreatic cell viability after rinsing with the two media is ≤5%. The medium can be selected based on the existing inventory in the laboratory without deliberate replacement. If the subsequent experiment is cell culture, it is recommended to prioritize the medium consistent with the culture system to reduce the cell adaptation cost.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e7.    Q: Step 12 mentions that \"BA3311 Red Blood Cell Lysis Buffer can be used to remove red blood cells\". After which step should this operation be performed? What should be noted during lysis to avoid damaging pancreatic cells?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: It should be performed after Step 10 (after two washes) and before Step 11 (before resuspension). Specific operation: After discarding the supernatant in Step 10, add 1mL of BA3311 Red Blood Cell Lysis Buffer, incubate at 4℃ for 5 minutes (avoid room-temperature incubation to prevent damage to pancreatic cells), then centrifuge at 4℃, 300×g for 5 minutes, discard the supernatant, and resuspend with 5mL of PBS containing 5% FBS (add an additional wash), then proceed to Step 11. Precautions: The lysis time should not exceed 8 minutes; otherwise, the pancreatic cell membrane will be damaged. If there are many red blood cells, lysis can be repeated once, but an additional wash is required to avoid residual lysis buffer affecting subsequent experiments (e.g., cell capture efficiency in single-cell sequencing).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e8.    Q: The pancreatic tissue dissociation solution needs to be stored at -80℃. If the ice pack melts during transportation and the reagent is left at 4℃ for 2 hours, can it still be used? What impact does repeated freezing and thawing have?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: It can still be used after being left at 4℃ for 2 hours, but it must be immediately returned to -80℃ and fully mixed before subsequent use. The enzyme in the dissociation solution loses ≤10% of its activity when placed at 4℃ for a short period (≤2 hours), which does not affect the dissociation effect. Repeated freezing and thawing will lead to a significant decrease in enzyme activity: Each freeze-thaw cycle reduces enzyme activity by 12%-18%; after more than 3 freeze-thaw cycles, the activity is less than 50%, which cannot effectively decompose the extracellular matrix of pancreatic tissue, resulting in a large number of tissue clumps after dissociation. It is recommended that after receiving the kit, aliquot the 4×3mL dissociation solution into 3mL\/tube (no further aliquoting is needed, as the original packaging is already in single-use volume), seal it, store at -80℃, and take one tube for each experiment to avoid repeated freezing and thawing.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e9.    Q: After quality control in Step 13, it is required to \"carry out subsequent experiments immediately\". If subsequent experiments cannot be conducted immediately, can the prepared pancreatic cell suspension be stored for a short period? What are the restrictions on storage conditions and time?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Short-term storage is possible under the conditions of sealed storage in a 4℃ refrigerator for no more than 1.5 hours, with repeated shaking avoided. During storage, the cell concentration should be adjusted to 1×10⁶-1×10⁷ cells\/mL using PBS containing 5% FBS, and the suspension should be placed in a low-adhesion centrifuge tube. After 1.5 hours, the viability of pancreatic cells (especially islet cells) will decrease significantly (10%-12% per hour), and cell aggregation is prone to occur; if stored for more than 3 hours, the cell viability may be lower than 50%, making it unsuitable for single-cell sequencing or cell culture. Before use, re-quality inspection is required, and only cells with viability ≥65% can be used. Additionally, gently pipette 5-8 times to disperse slightly aggregated cells.\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"8 Reactions\/kit","offer_id":46299524497620,"sku":"FG-BA3314-8","price":889.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/files\/BA3314.png?v=1774421468"},{"product_id":"heart-dissociation-kit-fg-ba3327","title":"FireGene Heart Dissociation Kit - Cardiomyocyte-Ready","description":"\u003ch3 id=\"overview\"\u003eOverview\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Heart Dissociation Kit\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eis a specialized enzymatic solution optimized for the dissociation of heart tissue into high-viability single-cell suspensions. This kit is ideal for cardiology research, enabling precise cellular analysis in conditions such as cardiomyopathy, heart failure, and cardiac regeneration.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch3 id=\"background-information\"\u003eBackground Information\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eDriven by Clinical and Scientific Research Needs:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSingle-cell sequencing of heart tissue is essential for understanding the\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ecellular complexity of cardiovascular diseases\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eTraditional methods struggle to isolate diverse cardiac cells including\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ecardiomyocytes, fibroblasts, endothelial cells, and immune populations\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eThis kit supports:\n\u003cul\u003e\n\u003cli\u003eAnalysis of\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ecellular heterogeneity and intercellular signaling\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eDiscovery of\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ebiomarkers and drug targets\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003erelated to heart failure and inflammation.\u003c\/li\u003e\n\u003cli\u003eResearch into\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eheart regeneration, development, and personalized therapy\u003c\/strong\u003e.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBackground of Technological Development:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eConventional dissociation methods often result in\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003elow cell viability\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eand incomplete tissue breakdown.\u003c\/li\u003e\n\u003cli\u003eFireGene improves outcomes through:\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eAdvanced enzymatic blends\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003etailored for dense cardiac ECM.\u003c\/li\u003e\n\u003cli\u003eCarefully optimized\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003econcentration, temperature, and reaction time\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eprotocols.\u003c\/li\u003e\n\u003cli\u003eA workflow that ensures\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ereproducible, high-yield single-cell preparation\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003efor downstream applications.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch3 id=\"detection-principle\"\u003eDetection Principle\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eImplements a\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003estepwise enzymatic digestion process\u003c\/strong\u003e:\n\u003cul\u003e\n\u003cli\u003eHeart tissue is\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eminced into small fragments\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eEnzyme reagents are added in sequence under\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eprecisely managed incubation conditions\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eThe digestion gently breaks down the\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eextracellular matrix and cell junctions\u003c\/strong\u003e, releasing intact cells.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003eResult:\n\u003cul\u003e\n\u003cli\u003eA\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eclean, viable single-cell suspension\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003esuitable for scRNA-seq, cardiac lineage tracing, functional assays, and drug screening.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 id=\"detection-principle\"\u003eSpecifications\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eSingle-cell sequencing, cell culture or other cell-related detections\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eCompatible Sample Types\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eHeart tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eSupported Instruments\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eWater bath, horizontal centrifuge, cell counter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e-20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eShelf-life\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 id=\"detection-principle\"\u003eKit Components\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e10 Reactions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 60%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003e10 Reactions\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e\n\u003cspan\u003eHeart DS 1 (Heart Dissociation\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003esolution 1)\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e1*5.1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e\n\u003cspan\u003eHeart DS 2 (Heart Dissociation\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003esolution 2\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e1*3 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e50 Reactions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 60%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003e50 Reactions\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e\n\u003cspan\u003eHeart DS 1 (Heart Dissociation\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003esolution 1)\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e5*5.1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e\n\u003cspan\u003eHeart DS 2 (Heart Dissociation\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003esolution 2）\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid #ddd;\"\u003e5*3 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eProduct FAQ\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e1.    Q: When dissociating heart tissue, according to the procedure, first add Dissociation Buffer 1 and incubate for 20 minutes, then add Dissociation Buffer 2. It is found that cell viability drops rapidly (from 80% to 50%) after adding Dissociation Buffer 2. What is the reason, and how to adjust the addition method of the two dissociation buffers?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The viability decline is because Dissociation Buffer 2 contains specific enzymes targeting cardiac collagen fibers, and direct addition easily stimulates exposed cardiomyocytes. Adjustment methods: ① Before adding Dissociation Buffer 2, take the centrifuge tube out of the 37℃ environment and cool it on wet ice for 1 minute to reduce the cell metabolic rate; ② Dilute 300μL of Dissociation Buffer 2 with 200μL of RPMI 1640 medium, then slowly drop it into the tube while gently pipetting (avoid violence). This can reduce direct enzyme damage to cardiomyocytes and maintain cell viability above 70%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e2.    Q: When dissociating heart tissue from mice with myocardial infarction model, the infarcted area tissue is found to be hard. After 35 minutes of enzymolysis, there are still a large number of tissue blocks, and the single-cell yield is extremely low. How to handle the hard tissue in the infarcted area?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The infarcted area is hard due to fibrous hyperplasia, so a \"pretreatment\" step needs to be added: ① Separately mince the infarcted area tissue into pieces smaller than 0.1mm³ (half the size of normal tissue), soak it in PBS for 5 minutes, and repeatedly squeeze the tissue pieces during this period to remove some necrotic substances; ② Increase the dosage of Dissociation Buffer 1 to 600μL (510μL for regular use) and Dissociation Buffer 2 to 350μL during enzymolysis, and extend the incubation time of Dissociation Buffer 1 to 25 minutes. This can increase the decomposition rate of tissue blocks in the infarcted area by 70% and improve the single-cell yield by 50%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e3.    Q: In Step 11, add three volumes of red blood cell lysis buffer (about 3mL) and incubate on ice for 10 minutes. For heart tissue with low blood content (such as the heart of juvenile mice), it is found that cardiomyocyte viability drops from 75% to 45% after lysis. How to optimize the red blood cell lysis operation?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Heart tissue with low blood content does not require full-volume lysis. Optimization methods: ① Adjust the dosage of lysis buffer according to the blood content of the tissue; the heart of juvenile mice only needs 1.5 volumes (about 1.5mL) of lysis buffer; ② Shorten the incubation time to 5-6 minutes, observe under a microscope every 2 minutes, and terminate immediately when red blood cells are basically lysed; ③ Wash twice with PBS containing 10% FBS after lysis (once for regular use) to fully remove residual lysis buffer. This can maintain cardiomyocyte viability above 65%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e4.    Q: When using a hybridization oven for enzymolysis, the operation is carried out at a rotation speed of 20-30 rpm, but it is found that the liquid in the centrifuge tube splashes onto the tube cap, causing some tissue to adhere to the cap and fail to be enzymolyzed. What is the reason, and how to prevent liquid splashing?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Liquid splashing is because heart tissue contains elastic fibers, which easily drive the liquid to oscillate violently when the hybridization oven rotates. Prevention methods: ① Control the liquid volume in the centrifuge tube within 3mL (3mL for regular use, which can be reduced to 2.8mL by decreasing the dosage of Dissociation Buffer 1 or medium); ② Stick a piece of sterile paraffin film on the inner side of the centrifuge tube cap; even if liquid splashes, it will adhere to the paraffin film. After enzymolysis, rinse the paraffin film with a small amount of medium to wash the tissue pieces back into the tube; ③ Reduce the rotation speed of the hybridization oven to 15-20 rpm to decrease the liquid oscillation amplitude, which can completely avoid splashing.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e5.    Q: After opening Dissociation Buffer 1 and Dissociation Buffer 2 in the kit, they are stored at -20℃ for 4 months. When used, it is found that the enzymolysis efficiency decreases (cell yield is only 60% of the original). Is the reagent invalid, and how to judge whether the reagent is still usable?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The validity period of the opened dissociation buffers stored at -20℃ is 3 months (2 years for unopened ones), and 4 months may lead to decreased enzyme activity. Judgment methods: ① Take 50μL of Dissociation Buffer 1 and 30μL of Dissociation Buffer 2, mix them, add a small amount of minced heart tissue, and incubate at 37℃ for 25 minutes. If the tissue pieces become significantly smaller and cells are released, it means the reagents are still usable; ② If the enzyme activity is insufficient, increase the dosage of both dissociation buffers by 20% and extend the total enzymolysis time to 40 minutes to supplement enzyme activity. This can restore the cell yield to about 80% of the original (not recommended for high-precision experiments such as single-cell sequencing).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e6.    Q: In Step 15, when filtering with a 20μm cell sieve, a large number of cardiomyocytes (with a diameter of about 15-20μm) are found to be retained, resulting in the loss of target cells. What is the reason, and how to adjust the filtration operation?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The retention of cardiomyocytes is mostly due to slight cell clumping or sieve pore deviation. Adjustment methods: ① Before filtration, gently pipette the cell suspension 20 times with a 1mL low-adhesion pipette tip to disperse small clumps with a diameter \u0026lt;20μm; ② Pre-wet the 20μm cell sieve with PBS containing 5% FBS to reduce cell adsorption to the sieve; ③ Slowly push the liquid with a 5mL syringe during filtration to avoid cell compression and deformation caused by sieve clogging. This can reduce the cardiomyocyte retention rate from 50% to below 10%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e7.    Q: After dissociation, a large number of spindle-shaped cell debris (suspected to be myocardial fiber debris) appear in the cell suspension, interfering with cell counting. How to remove these fiber debris?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Fiber debris is a product of incomplete enzymolysis of heart tissue. Removal methods: ① Before filtration after enzymolysis, centrifuge the cell suspension at 4℃, 200×g for 3 minutes, and discard the floating fiber debris in the upper layer; ② When filtering with a 70μm cell sieve, rinse the sieve repeatedly 3 times with PBS containing 2% FBS to ensure fiber debris flows out with the filtrate; ③ If there is still residue, filter again with a 20μm cell sieve (only retain the filtrate). This can remove more than 90% of fiber debris without affecting the cardiomyocyte recovery rate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e8.    Q: The centrifugation parameters in Steps 9 and 13 are both \"4℃, 300×g for 5 minutes\", but after centrifugation, the cardiomyocyte pellet is loose, and cells are easily sucked away when discarding the supernatant. What is the reason, and how to obtain a dense pellet?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The loose cardiomyocyte pellet is because the cell surface is negatively charged, leading to mutual repulsion and difficulty in aggregation. Solutions: ① Increase the centrifugation speed to 350×g and extend the centrifugation time to 8 minutes to enhance cell sedimentation capacity; ② Before centrifugation, add 5μL of 1% polylysine (self-prepared, non-toxic to cells) to the cell suspension to neutralize the cell surface charge and promote cell aggregation; ③ Retain 50μL of supernatant after centrifugation and mix it with the pellet to avoid the loss of small-volume cells with the supernatant. This can increase the pellet density by 60%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e9.    Q: When dissociating heart tissue from newborn rats (within 7 days of birth), a large number of cells tend to die (viability is only 35%), which is much lower than 70% of adult rats. What is the reason, and how to adjust the operation to protect immature cardiomyocytes?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The cell membrane of immature cardiomyocytes is fragile and easily damaged by enzymolysis solution and mechanical force. Adjustment methods: ① Shorten the incubation time of Dissociation Buffer 1 from 20 minutes to 15 minutes, and the incubation time of Dissociation Buffer 2 to 3-4 minutes to reduce enzyme damage to cells; ② Use a water bath for enzymolysis, and gently shake every 5 minutes (avoid violent oscillation) to reduce mechanical impact; ③ In the washing step, replace the regular PBS containing 5% FBS with PBS containing 10% FBS to enhance cell membrane protection. This can increase the viability of immature cardiomyocytes to above 60%.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e10.    Q: In Steps 7 and 8, rinse the centrifuge tube twice to collect a total of 9mL of filtrate. If one rinsing step is omitted and only 6mL of filtrate is collected, what impact will it have on the cell yield? Can the cell loss be compensated by other methods?\u003c\/strong\u003e\u003c\/span\u003e\u003cbr\u003e\u003cspan\u003eA: Omitting one rinsing step will cause about 25% of cardiomyocytes to remain on the inner wall of the centrifuge tube, reducing the cell yield by 30%. Compensation methods: ① If rinsing has been omitted, immediately rinse the centrifuge tube once with 3mL of medium, filter and combine the filtrate; ② In subsequent experiments, mince the tissue more fully (smaller than 0.2mm³) and extend the incubation time of Dissociation Buffer 1 to 22 minutes to increase cell release. This can compensate for the loss to a certain extent, but it is still recommended to rinse twice according to the standard procedure to ensure full cell recovery.\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"1 reaction\/kit","offer_id":47833521324244,"sku":"FG-BA3327-1rxns","price":59.0,"currency_code":"USD","in_stock":true},{"title":"10 reactions\/kit","offer_id":46299524530388,"sku":"FG-BA3327-10rxns","price":439.0,"currency_code":"USD","in_stock":true},{"title":"50 reactions\/kit","offer_id":47709044146388,"sku":"FG-BA3327-50rxns","price":1319.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/files\/BA3327.png?v=1774257345"},{"product_id":"gastrointestinal-dissociation-kit-fg-ba3316","title":"FireGene Gastrointestinal Dissociation Kit - Gut Cell Isolation","description":"\u003ch3 id=\"overview\"\u003eOverview\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Gastrointestinal Dissociation Kit\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eis a high-performance enzymatic solution designed to isolate viable single-cell suspensions from GI tissues. Optimized for applications like single-cell sequencing, immune profiling, and gut microbiota research, this kit delivers exceptional results for complex and fragile gastrointestinal samples.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch3 id=\"background-information\"\u003eBackground Information\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eDriven by Clinical and Scientific Research Needs:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSingle-cell sequencing of GI tissue is critical for understanding diseases such as\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eIBD (inflammatory bowel disease)\u003c\/strong\u003e,\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ecolorectal cancer\u003c\/strong\u003e, and\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eintestinal fibrosis\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eConventional methods do not fully preserve the\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ediversity of epithelial, stromal, and immune cells\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eThis kit enables:\n\u003cul\u003e\n\u003cli\u003eDetailed characterization of\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ecell subsets involved in inflammation, cancer, and tissue repair\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eIdentification of biomarkers and therapeutic targets for\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eprecision medicine\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eSupport for regenerative and microbiome-associated GI research.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBackground of Technological Development:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eTraditional techniques often result in\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003elow cell viability and inefficient dissociation\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli\u003eFireGene resolves these limitations by:\n\u003cul\u003e\n\u003cli\u003eUsing a\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003etargeted enzymatic system\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003etailored for gut ECM and cell junctions.\u003c\/li\u003e\n\u003cli\u003eOptimizing\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ereaction conditions\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003efor reproducibility across different GI tissue types.\u003c\/li\u003e\n\u003cli\u003eDelivering high cell recovery rates suitable for\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003esensitive downstream applications\u003c\/strong\u003e.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch3 id=\"detection-principle\"\u003eDetection Principle\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eUses a\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003emulti-step enzymatic digestion approach\u003c\/strong\u003e:\n\u003cul\u003e\n\u003cli\u003eGI tissue is\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ecut into small fragments\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003efor uniform reagent exposure.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEnzymes are applied sequentially\u003c\/strong\u003e, breaking down extracellular matrix and cellular adhesions.\u003c\/li\u003e\n\u003cli\u003eThe process occurs under\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ecarefully regulated incubation conditions\u003c\/strong\u003e.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003eOutcome:\n\u003cul\u003e\n\u003cli\u003eA\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003ehigh-quality, viable single-cell suspension\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eideal for scRNA-seq, flow cytometry, and functional GI assays.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 id=\"detection-principle\"\u003eSpecifications\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eApplications\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eSingle-cell sequencing, cell culture or other cell-related detections\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eCompatible Sample Types\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eGastrointestinal tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eSupported Instruments\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003eWater bath, horizontal centrifuge, cell counter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e-20 °C \/ 4 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 40%; font-weight: bold; padding: 8px; border: 1px solid #ddd;\"\u003eShelf-life\u003c\/td\u003e\n\u003ctd style=\"width: 60%; padding: 8px; border: 1px solid #ddd;\"\u003e24 months at -20 °C\u003cbr\u003e12 months at 4 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 id=\"detection-principle\"\u003eKit Components\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e10 Reactions\/kit\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px; height: 78.3752px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003cth style=\"width: 25.0447%; padding: 8px; border: 1px solid rgb(221, 221, 221); background: rgb(240, 240, 240); height: 19.5938px;\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"width: 25.0447%; padding: 8px; border: 1px solid rgb(221, 221, 221); background: rgb(240, 240, 240); height: 19.5938px;\"\u003eQuantity\u003c\/th\u003e\n\u003cth style=\"width: 25.0447%; padding: 8px; border: 1px solid rgb(221, 221, 221); background: rgb(240, 240, 240); height: 19.5938px;\"\u003eStorage\u003c\/th\u003e\n\u003cth style=\"width: 25.0447%; padding: 8px; border: 1px solid rgb(221, 221, 221); background: rgb(240, 240, 240); height: 19.5938px;\"\u003eShelf-life\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003eBuffer A\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003e40 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003e-20 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003eEnzyme B\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003e1 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003e-20 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003eEnzyme C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003e100 μL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003e4 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"background-color: rgb(255, 255, 0);\"\u003e\u003cstrong\u003e50 Reactions\/kit\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-family: Arial, sans-serif; font-size: 14px; height: 78.3752px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003cth style=\"width: 25.0447%; padding: 8px; border: 1px solid rgb(221, 221, 221); background: rgb(240, 240, 240); height: 19.5938px;\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"width: 25.0447%; padding: 8px; border: 1px solid rgb(221, 221, 221); background: rgb(240, 240, 240); height: 19.5938px;\"\u003eQuantity\u003c\/th\u003e\n\u003cth style=\"width: 25.0447%; padding: 8px; border: 1px solid rgb(221, 221, 221); background: rgb(240, 240, 240); height: 19.5938px;\"\u003eStorage\u003c\/th\u003e\n\u003cth style=\"width: 24.8658%; padding: 8px; border: 1px solid rgb(221, 221, 221); background: rgb(240, 240, 240); height: 19.5938px;\"\u003eShelf-life\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003eBuffer A\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003e5*40 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003e-20 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 24.8658%;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003eEnzyme B\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003e5*1 mL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003e-20 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 24.8658%;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003eEnzyme C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003e5*100 μL\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 25.0447%;\"\u003e4 °C\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; border: 1px solid rgb(221, 221, 221); height: 19.5938px; width: 24.8658%;\"\u003e12 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eProduct FAQ\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e1.    Q: Is this kit only suitable for mammalian gastrointestinal tissue? Are there differences in dissociation effects on tissues from different parts of the gastrointestinal tract (e.g., gastric mucosa, small intestinal villi, colonic mucosa)? Can it be used for non-mammalian gastrointestinal tissue (e.g., fish intestines)?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The kit is suitable for full-segment gastrointestinal tissue (stomach, small intestine, colon, etc.) of mammals such as humans and mice. There are slight differences in dissociation effects on tissues from different parts: small intestinal villi tissue is relatively fragile, so the digestion time is recommended to be shortened to 20-30 minutes to avoid villus structure breakage; gastric mucosa and colonic mucosa contain more collagen fibers, so the digestion time can be extended to 30-40 minutes, and dynamic adjustment is required through quality inspection. It is not suitable for non-mammalian gastrointestinal tissue. The cell membrane structure and fiber components of non-mammalian gastrointestinal cells (such as fish intestines) are significantly different from those of mammals. The enzyme system of the kit cannot accurately degrade their extracellular matrix, which easily leads to incomplete dissociation or a sharp drop in cell viability.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e2.    Q: The kit is labeled \"10 rxns\". Each experiment requires 4mL of Buffer A, 100μL of Enzyme B, and 10μL of Enzyme C. If only 100mg of gastrointestinal tissue (1\/2 of the standard dosage) is processed in a single experiment, can the reagent dosage be reduced proportionally?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: Proportional reduction of reagent dosage is not recommended. Buffer A is not only a reaction medium but also needs to maintain the tissue suspension environment; a volume of 4mL can ensure the full dispersion of tissue fragments. The ratio of Enzyme B to Enzyme C is precisely formulated; reducing the dosage will lead to insufficient enzyme concentration, which cannot effectively decompose the fibers of gastrointestinal tissue and instead increases the cell clumping rate. When processing 100mg of tissue, reagents should still be added according to the standard dosage. During subsequent centrifugation to collect cells, the centrifugation time can be extended (from 5 minutes to 8 minutes) to ensure sufficient cell precipitation and avoid cell loss due to the relatively large liquid volume.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e3.    Q: During digestion in Step 2, a water bath requires \"manual shaking every 3-5 minutes\", and a hybridization oven requires \"a rotation speed of 20-30 rpm\". What are the core differences in operation effects between the two devices? Which one is more suitable for gastrointestinal tissue dissociation?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: The core difference lies in \"the uniformity of enzyme-tissue contact\": the automatic rotation speed of the hybridization oven can keep gastrointestinal tissue fragments suspended in the dissociation solution at all times, avoiding local over-enzymolysis (e.g., tissue at the bottom of the tube) or insufficient enzymolysis (e.g., tissue at the liquid surface) caused by gravity sedimentation. If the manual shaking frequency of the water bath is inconsistent, it is easy to cause over-digestion of small intestinal villi tissue and incomplete dissociation of colonic mucosa tissue. In terms of effect, the hybridization oven is more suitable for gastrointestinal tissue dissociation, especially for fiber-rich tissues such as the colon, with a single-cell yield 18%-25% higher than that of the water bath. If a water bath is used, it is necessary to strictly shake at 3-minute intervals for 10 seconds each time to ensure no tissue settles at the bottom.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e4.    Q: Both Step 5 and Step 6 require filtration with a 70μm cell sieve. If the laboratory lacks a 70μm cell sieve, can it be replaced with a 50μm or 100μm cell sieve? What impact will the replacement have?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: A 50μm or 100μm cell sieve cannot be used as a replacement. The pore size of the 50μm sieve is too small and will retain normal single cells in gastrointestinal tissue (e.g., the diameter of small intestinal epithelial cells is about 12-18μm; although they can pass through, the sieve is easily blocked by fiber fragments, leading to filtration difficulties and even cell extrusion to reduce viability). The pore size of the 100μm sieve is too large and cannot effectively filter undigested tissue fragments (e.g., gastric mucosa fiber fragments). These fragments will enter subsequent steps along with cells, interfering with the accuracy of cell counting and may even block the single-cell sequencing chip. If a 70μm cell sieve is lacking, it is necessary to contact the supplier for emergency deployment or select a compatible 70μm cell sieve of the same brand (product number: BS70-C); random replacement is not allowed.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e5.    Q: The instruction manual mentions that \"DMEM medium can replace RPMI 1640 medium\". After replacement, is it necessary to adjust centrifugation parameters or digestion time? Is there any difference in the impact of the two media on the viability of gastrointestinal cells?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: There is no need to adjust centrifugation parameters (still 4℃, 300×g for 5 minutes) or digestion time after replacement. Both DMEM and RPMI 1640 are common basal media for mammalian cells. Although they differ in glucose and amino acid content, both can provide a suitable osmotic pressure (280-320mOsm\/kg) and pH (7.2-7.4) for gastrointestinal tissue dissociation, with no impact on enzymolysis efficiency. The impact on the viability of gastrointestinal cells is minimal: experimental data shows that the difference in cell viability after dissociating mouse small intestinal tissue with the two media is ≤6%. The medium can be selected based on the existing inventory in the laboratory without deliberate replacement.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cstrong\u003e6.    Q: Step 7 uses PBS containing 5% FBS to wash cells. If serum-free operation is required for the experiment (e.g., some cell sorting experiments), can PBS without FBS be used as a replacement? Or can serum substitutes (e.g., BSA) replace FBS?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: PBS without FBS cannot be used as a replacement. The core function of FBS is to neutralize residual enzyme activity (Enzyme B and Enzyme C) to prevent enzymes from continuing to damage cells; PBS without FBS cannot neutralize enzyme activity, which will lead to a more than 30% drop in cell viability after washing. If serum-free operation is required, PBS containing 1% BSA can be used to replace PBS containing 5% FBS. BSA can play a similar enzyme-neutralizing role and does not contain serum components; however, it should be noted that after replacing with BSA, the washing time needs to be extended from 5 minutes to 8 minutes to ensure complete neutralization of enzyme activity and avoid residues affecting subsequent experiments.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e7.    Q: Enzyme C needs to be stored at 4℃ in the dark. If it is left at room temperature for 30 minutes (e.g., forgotten to be put back in the refrigerator after use), can it still be used? What consequences will occur if Enzyme C is stored at -20℃ for a long time?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: It can still be used after being left at room temperature for 30 minutes, but it must be immediately put back in the 4℃ refrigerator and fully mixed before use. Enzyme C is an active enzyme preparation; short-term storage at room temperature (≤30 minutes) results in ≤10% activity loss, which does not affect the dissociation effect. Long-term storage of Enzyme C at -20℃ is not allowed. Low temperature will cause Enzyme C to denature and inactivate (after 24 hours of freezing at -20℃, activity decreases by more than 70%), making it unable to work synergistically with Enzyme B to decompose gastrointestinal tissue fibers, leading to dissociation failure. The correct storage condition for Enzyme C is 4℃ in the dark, with a validity period of 1 year, which must be strictly followed.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e8.    Q: If there are many red blood cells in the cell suspension after dissociation and \u003ca href=\"https:\/\/firegene.com\/products\/red-blood-cell-lysis-kit-fg-ba3311?_pos=12\u0026amp;_sid=c5ff19011\u0026amp;_ss=r\"\u003eFG-BA3311 Red Blood Cell Lysis Buffer\u003c\/a\u003e is needed to remove them, after which step should this operation be performed? What should be noted during lysis to avoid damaging gastrointestinal cells?\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003eA: This operation should be performed after Step 7 and before Step 8. Specific operation: After discarding the supernatant in Step 7, add 1mL of FG-BA3311 Red Blood Cell Lysis Buffer, incubate on ice for 5 minutes (to avoid damaging gastrointestinal cells due to room-temperature incubation), centrifuge at 4℃, 300×g for 5 minutes, discard the supernatant, then resuspend with 5mL of PBS containing 5% FBS (add an additional washing step), and then proceed to Step 8. Precautions: ① The lysis time should not exceed 8 minutes; ice incubation can reduce the toxicity of the lysis buffer to gastrointestinal cells; ② If there are too many red blood cells (e.g., gastric mucosa tissue contains a large number of capillaries), lysis can be repeated once, but an additional PBS washing step is required to avoid residual lysis buffer affecting subsequent experiments (e.g., interference with fluorescence signals in cell sorting).\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"2 reactions\/kit","offer_id":47833362956500,"sku":"FG-BA3316-2rxns","price":189.0,"currency_code":"USD","in_stock":true},{"title":"10 reactions\/kit","offer_id":46299524563156,"sku":"FG-BA3316-10rxns","price":759.0,"currency_code":"USD","in_stock":true},{"title":"50 reactions\/kit","offer_id":47718733316308,"sku":"FG-BA3316-50rxns","price":2759.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/files\/BA3316_8da3ff52-d799-4f95-a195-65af984be99f.png?v=1774513519"},{"product_id":"multi-tissue-dissociation-kit-plus","title":"FireGene Multi Tissue Dissociation Kit Plus for Single-Cell Preparation","description":"\u003cp\u003e\u003cstrong\u003eFireGene Multi Tissue Dissociation Kit Plus\u003c\/strong\u003e is designed for the isolation and preparation of single-cell suspensions from highly metabolically active and pathological mammalian tissue samples. The kit uses an enzyme-based workflow to gently and rapidly degrade extracellular matrix and release cells. Prepared single-cell suspensions are suitable for single-cell sequencing, cell culture, and other cell-related assays. The protocol supports fresh tissue processing with adjustable digestion time based on tissue type, species, and sample condition.\u003c\/p\u003e\n\u003ch2\u003eBackground Information\u003c\/h2\u003e\n\u003cp\u003eHighly active or pathological tissues can vary in matrix density, cellular composition, and tissue condition. A flexible dissociation workflow helps researchers adjust digestion time while preparing usable single-cell suspensions for downstream analysis.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Multi Tissue Dissociation Kit Plus for Single-Cell Preparation\u003c\/strong\u003e provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.\u003c\/p\u003e\n\u003ch3\u003eResearch Areas\u003c\/h3\u003e\n\u003cp\u003eThis product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eMammalian tissue biology\u003c\/li\u003e\n\u003cli\u003eDisease model research\u003c\/li\u003e\n\u003cli\u003ePathological tissue analysis\u003c\/li\u003e\n\u003cli\u003eSingle-cell biology\u003c\/li\u003e\n\u003cli\u003eCancer and inflammation research\u003c\/li\u003e\n\u003cli\u003eDrug discovery and screening\u003c\/li\u003e\n\u003cli\u003eCell culture workflow development\u003c\/li\u003e\n\u003cli\u003eTranslational biomedical research\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKey Applications\u003c\/h3\u003e\n\u003cp\u003eThe prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSingle-cell RNA sequencing\u003c\/li\u003e\n\u003cli\u003eFlow cytometry and cell sorting\u003c\/li\u003e\n\u003cli\u003ePrimary cell culture\u003c\/li\u003e\n\u003cli\u003eCell viability and cell counting\u003c\/li\u003e\n\u003cli\u003eCell-type composition analysis\u003c\/li\u003e\n\u003cli\u003eCell-based functional assays\u003c\/li\u003e\n\u003cli\u003eTissue heterogeneity studies\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eMulti Tissue Dissociation Kit (Plus)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBrand\u003c\/td\u003e\n\u003ctd\u003eFireGene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalog No.\u003c\/td\u003e\n\u003ctd\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3348\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit Size\u003c\/td\u003e\n\u003ctd\u003e10 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Type\u003c\/td\u003e\n\u003ctd\u003eHighly metabolically active and pathological mammalian tissue samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible Samples\u003c\/td\u003e\n\u003ctd\u003eFresh tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended Starting Material\u003c\/td\u003e\n\u003ctd\u003eApproximately 200 mg tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Function\u003c\/td\u003e\n\u003ctd\u003ePreparation of single-cell suspensions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWorkflow\u003c\/td\u003e\n\u003ctd\u003eEnzymatic digestion, filtration, centrifugation, washing, resuspension, quality control\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDownstream Applications\u003c\/td\u003e\n\u003ctd\u003eSingle-cell sequencing, cell culture, cell-related assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDigestion Conditions\u003c\/td\u003e\n\u003ctd\u003e37°C for 10–20 minutes; 15–40 minutes for pathological tissues\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Instruments\u003c\/td\u003e\n\u003ctd\u003eWater bath or hybridization oven, horizontal centrifuge\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Reagents\u003c\/td\u003e\n\u003ctd\u003ePBS, FBS, RPMI 1640 medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Consumables\u003c\/td\u003e\n\u003ctd\u003eLow-adhesion pipette tips, 5 mL and 15 mL centrifuge tubes, 70 μm cell strainers\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOptional Related Reagent\u003c\/td\u003e\n\u003ctd\u003eRed Blood Cell Lysis Solution, Catalog No. BA3311\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf Life\u003c\/td\u003e\n\u003ctd\u003eTwo years\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eResearch Use\u003c\/td\u003e\n\u003ctd\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eCatalog Number\u003c\/th\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eMTDSP (Multi Tissue Dissociation Solution (Plus))\u003c\/td\u003e\n\u003ctd\u003eFG-BA3348\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"FireGene","offers":[{"title":"10 rxns","offer_id":47868493955284,"sku":"FG-BA3348-10","price":619.0,"currency_code":"USD","in_stock":true},{"title":"50 rxns","offer_id":47868493988052,"sku":"FG-BA3348-50","price":2099.0,"currency_code":"USD","in_stock":true}]},{"product_id":"mouse-skin-dissociation-kit","title":"FireGene Mouse Skin Dissociation Kit for Single-Cell Preparation","description":"\u003cp\u003e\u003cstrong\u003eFireGene Mouse Skin Dissociation Kit\u003c\/strong\u003e is designed for enzymatic dissociation of mouse skin tissue and preparation of single-cell suspensions. The workflow addresses common challenges in mouse skin processing, including difficult dissociation and reduced cell viability. Prepared cells can be used for single-cell sequencing, cell sorting, cell culture, and related cellular experiments. The protocol uses a two-step enzymatic digestion workflow with filtration, washing, resuspension, and quality control.\u003c\/p\u003e\n\u003ch2\u003eBackground Information\u003c\/h2\u003e\n\u003cp\u003eMouse skin tissue contains layered epithelial, stromal, vascular, and immune components that can make dissociation challenging. A tissue-specific enzymatic workflow helps prepare cell suspensions for downstream cellular analysis while allowing digestion time to be adjusted by sample condition.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Mouse Skin Dissociation Kit for Single-Cell Preparation\u003c\/strong\u003e provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.\u003c\/p\u003e\n\u003ch3\u003eResearch Areas\u003c\/h3\u003e\n\u003cp\u003eThis product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eMouse skin biology\u003c\/li\u003e\n\u003cli\u003eDermatology research\u003c\/li\u003e\n\u003cli\u003eWound healing studies\u003c\/li\u003e\n\u003cli\u003eSkin inflammation and immunology\u003c\/li\u003e\n\u003cli\u003eBarrier tissue research\u003c\/li\u003e\n\u003cli\u003eCancer and tumor microenvironment studies\u003c\/li\u003e\n\u003cli\u003eSingle-cell biology\u003c\/li\u003e\n\u003cli\u003eCell culture assay development\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKey Applications\u003c\/h3\u003e\n\u003cp\u003eThe prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eMouse skin single-cell suspension preparation\u003c\/li\u003e\n\u003cli\u003eSingle-cell RNA sequencing\u003c\/li\u003e\n\u003cli\u003eFlow cytometry and cell sorting\u003c\/li\u003e\n\u003cli\u003ePrimary skin cell culture\u003c\/li\u003e\n\u003cli\u003eCell viability and counting\u003c\/li\u003e\n\u003cli\u003eSkin tissue heterogeneity analysis\u003c\/li\u003e\n\u003cli\u003eInflammation and immune profiling\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eSkin Dissociation Kit, mouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBrand\u003c\/td\u003e\n\u003ctd\u003eFireGene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalog No.\u003c\/td\u003e\n\u003ctd\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3340\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit Size\u003c\/td\u003e\n\u003ctd\u003e10 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Type\u003c\/td\u003e\n\u003ctd\u003eMouse skin tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible Samples\u003c\/td\u003e\n\u003ctd\u003eFresh mouse skin tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended Starting Material\u003c\/td\u003e\n\u003ctd\u003eApproximately 200 mg tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Function\u003c\/td\u003e\n\u003ctd\u003ePreparation of single-cell suspensions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWorkflow\u003c\/td\u003e\n\u003ctd\u003eTwo-step enzymatic digestion, filtration, centrifugation, washing, resuspension, quality control\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDownstream Applications\u003c\/td\u003e\n\u003ctd\u003eSingle-cell sequencing, cell sorting, cell culture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDigestion Conditions\u003c\/td\u003e\n\u003ctd\u003e37°C; 30 minutes with MSDS 1, then approximately 0.5–1 hour with MSDS 2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Instruments\u003c\/td\u003e\n\u003ctd\u003eWater bath or hybridization oven, horizontal centrifuge\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Reagents\u003c\/td\u003e\n\u003ctd\u003eRPMI 1640 medium, PBS, FBS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Consumables\u003c\/td\u003e\n\u003ctd\u003eLow-adsorption pipette tips, 5 mL and 15 mL centrifuge tubes, 70 μm cell strainers\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOptional Related Reagent\u003c\/td\u003e\n\u003ctd\u003eRed Blood Cell Lysis Solution, Catalog No. BA3311\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf Life\u003c\/td\u003e\n\u003ctd\u003eTwo years\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eResearch Use\u003c\/td\u003e\n\u003ctd\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eCatalog Number\u003c\/th\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eMSDS1 (Mouse Skin Dissociation Solution 1)\u003c\/td\u003e\n\u003ctd\u003eFG-BA3340-A\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMSDS2 (Mouse Skin Dissociation Solution 2)\u003c\/td\u003e\n\u003ctd\u003eFG-BA3340-B\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e3 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"FireGene","offers":[{"title":"10 rxns","offer_id":47868494840020,"sku":"FG-BA3340-10","price":459.0,"currency_code":"USD","in_stock":true},{"title":"50 rxns","offer_id":47868494872788,"sku":"FG-BA3340-50","price":1439.0,"currency_code":"USD","in_stock":true}]},{"product_id":"blood-vascular-dissociation-kit-plus","title":"FireGene Blood Vascular Dissociation Kit Plus for Single-Cell Preparation","description":"\u003cp\u003e\u003cstrong\u003eFireGene Blood Vascular Dissociation Kit Plus\u003c\/strong\u003e is an optimized enzyme-based kit for dissociation of blood vascular tissues from humans and other large mammals. The workflow is designed to improve dissociation efficiency while preserving cell viability, helping address the challenges of vascular tissue processing. Prepared cells can be used for single-cell sequencing, cell sorting, cell culture, and other cellular experiments. The protocol uses a two-step digestion workflow with quality checks during processing.\u003c\/p\u003e\n\u003ch2\u003eBackground Information\u003c\/h2\u003e\n\u003cp\u003eVascular tissues can be difficult to dissociate because of extracellular matrix structure and tissue-specific cellular organization. A vascular-focused dissociation workflow helps researchers prepare single-cell suspensions for studying endothelial cells, smooth muscle cells, immune cells, and other vascular cell populations.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Blood Vascular Dissociation Kit Plus for Single-Cell Preparation\u003c\/strong\u003e provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.\u003c\/p\u003e\n\u003ch3\u003eResearch Areas\u003c\/h3\u003e\n\u003cp\u003eThis product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eVascular biology\u003c\/li\u003e\n\u003cli\u003eCardiovascular disease research\u003c\/li\u003e\n\u003cli\u003eEndothelial cell biology\u003c\/li\u003e\n\u003cli\u003eSmooth muscle cell research\u003c\/li\u003e\n\u003cli\u003eAtherosclerosis and vascular inflammation\u003c\/li\u003e\n\u003cli\u003eAngiogenesis research\u003c\/li\u003e\n\u003cli\u003eTissue remodeling studies\u003c\/li\u003e\n\u003cli\u003eSingle-cell biology\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKey Applications\u003c\/h3\u003e\n\u003cp\u003eThe prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eVascular tissue single-cell suspension preparation\u003c\/li\u003e\n\u003cli\u003eSingle-cell sequencing\u003c\/li\u003e\n\u003cli\u003eFlow cytometry and cell sorting\u003c\/li\u003e\n\u003cli\u003ePrimary vascular cell culture\u003c\/li\u003e\n\u003cli\u003eCell viability and cell counting\u003c\/li\u003e\n\u003cli\u003eVascular tissue heterogeneity analysis\u003c\/li\u003e\n\u003cli\u003eCardiovascular disease model research\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eBlood Vascular Dissociation Kit (Plus)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBrand\u003c\/td\u003e\n\u003ctd\u003eFireGene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalog No.\u003c\/td\u003e\n\u003ctd\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3337\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit Size\u003c\/td\u003e\n\u003ctd\u003e10 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Type\u003c\/td\u003e\n\u003ctd\u003eBlood vascular tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible Samples\u003c\/td\u003e\n\u003ctd\u003eFresh vascular tissues from humans and other large mammals\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended Starting Material\u003c\/td\u003e\n\u003ctd\u003eApproximately 200 mg tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Function\u003c\/td\u003e\n\u003ctd\u003ePreparation of vascular tissue single-cell suspensions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWorkflow\u003c\/td\u003e\n\u003ctd\u003eTwo-step enzymatic digestion, dilution, filtration, centrifugation, washing, resuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDownstream Applications\u003c\/td\u003e\n\u003ctd\u003eSingle-cell sequencing, cell sorting, cell culture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDigestion Conditions\u003c\/td\u003e\n\u003ctd\u003e37°C; 20–30 minutes with BVDSP 1, then 30 minutes to 2 hours with BVDSP 2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Instruments\u003c\/td\u003e\n\u003ctd\u003eWater bath or hybridization oven, horizontal centrifuge\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Reagents\u003c\/td\u003e\n\u003ctd\u003ePBS, FBS, RPMI 1640 medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Consumables\u003c\/td\u003e\n\u003ctd\u003eLow-adhesion pipette tips, 5 mL and 15 mL centrifuge tubes, 70 μm cell strainers\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOptional Related Reagent\u003c\/td\u003e\n\u003ctd\u003eRed Blood Cell Lysis Solution, Catalog No. BA3311\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf Life\u003c\/td\u003e\n\u003ctd\u003eTwo years\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eResearch Use\u003c\/td\u003e\n\u003ctd\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eCatalog Number\u003c\/th\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eBVDSP 1 (Blood Vascular Dissociation Solution (Plus) 1)\u003c\/td\u003e\n\u003ctd\u003eFG-BA3337-A\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBVDSP 2 (Blood Vascular Dissociation Solution (Plus) 2)\u003c\/td\u003e\n\u003ctd\u003eFG-BA3337-A\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"FireGene","offers":[{"title":"10 rxns","offer_id":47868495036628,"sku":"FG-BA3337-10","price":2099.0,"currency_code":"USD","in_stock":true},{"title":"50 rxns","offer_id":47868495069396,"sku":"FG-BA3337-50","price":7999.0,"currency_code":"USD","in_stock":true}]},{"product_id":"tumor-dissociation-kit-plus","title":"FireGene Tumor Dissociation Kit Plus for Single-Cell Preparation","description":"\u003cp\u003e\u003cstrong\u003eFireGene Tumor Dissociation Kit Plus\u003c\/strong\u003e is designed for the isolation and preparation of single-cell suspensions from complex mammalian tumor tissues. The kit uses enzymes to gently and rapidly break down extracellular matrix and release cells. Prepared tumor cell suspensions can be used for single-cell sequencing, cell culture, and other cell-related assays. Digestion time can be adjusted based on species, tissue amount, and tumor condition, including samples with higher necrosis.\u003c\/p\u003e\n\u003ch2\u003eBackground Information\u003c\/h2\u003e\n\u003cp\u003eTumor tissues often contain diverse cell populations, dense extracellular matrix, stromal components, necrotic regions, and immune cell infiltration. A tumor-focused dissociation workflow helps prepare single-cell suspensions for studying tumor heterogeneity and the tumor microenvironment.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Tumor Dissociation Kit Plus for Single-Cell Preparation\u003c\/strong\u003e provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.\u003c\/p\u003e\n\u003ch3\u003eResearch Areas\u003c\/h3\u003e\n\u003cp\u003eThis product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eCancer biology\u003c\/li\u003e\n\u003cli\u003eTumor microenvironment research\u003c\/li\u003e\n\u003cli\u003eCancer immunology\u003c\/li\u003e\n\u003cli\u003eDrug discovery and screening\u003c\/li\u003e\n\u003cli\u003eTranslational oncology\u003c\/li\u003e\n\u003cli\u003eTumor heterogeneity studies\u003c\/li\u003e\n\u003cli\u003eSingle-cell biology\u003c\/li\u003e\n\u003cli\u003eCell culture assay development\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKey Applications\u003c\/h3\u003e\n\u003cp\u003eThe prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eTumor single-cell suspension preparation\u003c\/li\u003e\n\u003cli\u003eSingle-cell RNA sequencing\u003c\/li\u003e\n\u003cli\u003eFlow cytometry and cell sorting\u003c\/li\u003e\n\u003cli\u003ePrimary tumor cell culture\u003c\/li\u003e\n\u003cli\u003eCell viability and counting\u003c\/li\u003e\n\u003cli\u003eTumor microenvironment profiling\u003c\/li\u003e\n\u003cli\u003eDrug response and biomarker studies\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eTumor Dissociation Kit (Plus)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBrand\u003c\/td\u003e\n\u003ctd\u003eFireGene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalog No.\u003c\/td\u003e\n\u003ctd\u003eFG-BA3349\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit Size\u003c\/td\u003e\n\u003ctd\u003e10 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Type\u003c\/td\u003e\n\u003ctd\u003eComplex mammalian tumor tissues\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible Samples\u003c\/td\u003e\n\u003ctd\u003eFresh tumor tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended Starting Material\u003c\/td\u003e\n\u003ctd\u003eApproximately 200 mg tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Function\u003c\/td\u003e\n\u003ctd\u003ePreparation of tumor tissue single-cell suspensions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWorkflow\u003c\/td\u003e\n\u003ctd\u003eEnzymatic digestion, filtration, centrifugation, washing, resuspension, quality control\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDownstream Applications\u003c\/td\u003e\n\u003ctd\u003eSingle-cell sequencing, cell culture, cell-related assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDigestion Conditions\u003c\/td\u003e\n\u003ctd\u003e37°C for 10–40 minutes; longer for samples with high necrosis\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Instruments\u003c\/td\u003e\n\u003ctd\u003eWater bath or hybridization oven, horizontal centrifuge\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Reagents\u003c\/td\u003e\n\u003ctd\u003ePBS, FBS, RPMI 1640 medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Consumables\u003c\/td\u003e\n\u003ctd\u003eLow-adhesion pipette tips, 5 mL and 15 mL centrifuge tubes, 70 μm cell strainers\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOptional Related Reagent\u003c\/td\u003e\n\u003ctd\u003eRed Blood Cell Lysis Solution, Catalog No. BA3311\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf Life\u003c\/td\u003e\n\u003ctd\u003eTwo years\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eResearch Use\u003c\/td\u003e\n\u003ctd\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eCatalog Number\u003c\/th\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eTDSP (Tumor Dissociation Solution (Plus))\u003c\/td\u003e\n\u003ctd\u003eFG-BA3349\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"FireGene","offers":[{"title":"10 rxns","offer_id":47868495102164,"sku":"FG-BA3349-10","price":619.0,"currency_code":"USD","in_stock":true},{"title":"50 rxns","offer_id":47868495134932,"sku":"FG-BA3349-50","price":1769.0,"currency_code":"USD","in_stock":true}]},{"product_id":"spinal-cord-dissociation-kit","title":"FireGene Spinal Cord Dissociation Kit for Single-Cell Preparation","description":"\u003cp\u003e\u003cstrong\u003eFireGene Spinal Cord Dissociation Kit\u003c\/strong\u003e is designed for the isolation and preparation of single-cell suspensions from spinal cord tissue. The kit uses enzyme-based digestion to gently and rapidly disrupt extracellular matrix and release cells. The workflow includes digestion, filtration, washing, debris removal, optional red blood cell lysis, and quality control. Prepared cell suspensions can be used for single-cell sequencing, cell culture, and other cell-related assays.\u003c\/p\u003e\n\u003ch2\u003eBackground Information\u003c\/h2\u003e\n\u003cp\u003eSpinal cord tissue can contain fragile neural and glial cell populations along with debris generated during dissociation. A workflow that combines enzymatic digestion with debris removal helps improve sample clarity before downstream cellular analysis.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Spinal Cord Dissociation Kit for Single-Cell Preparation\u003c\/strong\u003e provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.\u003c\/p\u003e\n\u003ch3\u003eResearch Areas\u003c\/h3\u003e\n\u003cp\u003eThis product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eNeuroscience research\u003c\/li\u003e\n\u003cli\u003eSpinal cord injury models\u003c\/li\u003e\n\u003cli\u003eNeuroinflammation studies\u003c\/li\u003e\n\u003cli\u003eNeurodegeneration research\u003c\/li\u003e\n\u003cli\u003eGlial cell biology\u003c\/li\u003e\n\u003cli\u003eRegenerative medicine\u003c\/li\u003e\n\u003cli\u003eSingle-cell biology\u003c\/li\u003e\n\u003cli\u003eCell culture assay development\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKey Applications\u003c\/h3\u003e\n\u003cp\u003eThe prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSpinal cord single-cell suspension preparation\u003c\/li\u003e\n\u003cli\u003eSingle-cell sequencing\u003c\/li\u003e\n\u003cli\u003eFlow cytometry and cell sorting\u003c\/li\u003e\n\u003cli\u003ePrimary neural cell culture\u003c\/li\u003e\n\u003cli\u003eCell viability and counting\u003c\/li\u003e\n\u003cli\u003eDebris reduction after tissue dissociation\u003c\/li\u003e\n\u003cli\u003eCell-type composition analysis\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eSpinal Cord Dissociation Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBrand\u003c\/td\u003e\n\u003ctd\u003eFireGene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalog No.\u003c\/td\u003e\n\u003ctd\u003eFG-BA3334\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit Size\u003c\/td\u003e\n\u003ctd\u003e10 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Type\u003c\/td\u003e\n\u003ctd\u003eSpinal cord tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible Samples\u003c\/td\u003e\n\u003ctd\u003eFresh spinal cord tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended Starting Material\u003c\/td\u003e\n\u003ctd\u003eApproximately 200 mg tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Function\u003c\/td\u003e\n\u003ctd\u003ePreparation of spinal cord single-cell suspensions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWorkflow\u003c\/td\u003e\n\u003ctd\u003eEnzymatic digestion, filtration, centrifugation, debris removal, washing, optional RBC lysis, resuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDownstream Applications\u003c\/td\u003e\n\u003ctd\u003eSingle-cell sequencing, cell culture, cell-related assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDigestion Conditions\u003c\/td\u003e\n\u003ctd\u003e37°C for 20–30 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Instruments\u003c\/td\u003e\n\u003ctd\u003eWater bath or hybridization oven, horizontal centrifuge\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Reagents\u003c\/td\u003e\n\u003ctd\u003ePBS, FBS, RPMI 1640 medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Consumables\u003c\/td\u003e\n\u003ctd\u003eLow-adhesion pipette tips, centrifuge tubes, 20 μm and 70 μm cell strainers\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOptional Related Reagent\u003c\/td\u003e\n\u003ctd\u003eRed Blood Cell Lysis Solution, Catalog No. BA3311\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eBuffer A and Enzyme B: -20°C; Enzyme C and DRS: 4°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf Life\u003c\/td\u003e\n\u003ctd\u003eBuffer A and Enzyme B: 2 years; Enzyme C and DRS: 1 year\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eResearch Use\u003c\/td\u003e\n\u003ctd\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eCatalog Number\u003c\/th\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer A\u003c\/td\u003e\n\u003ctd\u003eFG-BA3334-A\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e21.6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEnzyme B\u003c\/td\u003e\n\u003ctd\u003eFG-BA3334-B\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e8 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEnzyme C\u003c\/td\u003e\n\u003ctd\u003eFG-BA3334-C\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e100 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDRS (Spinal Cord Cell Debris Removal Solution)\u003c\/td\u003e\n\u003ctd\u003eFG-BA3308-D\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"FireGene","offers":[{"title":"10 rxns","offer_id":47868495167700,"sku":"FG-BA3334-10","price":359.0,"currency_code":"USD","in_stock":true},{"title":"50 rxns","offer_id":47868495200468,"sku":"FG-BA3334-50","price":1119.0,"currency_code":"USD","in_stock":true}]},{"product_id":"fallopian-tube-dissociation-kit","title":"FireGene Fallopian Tube Dissociation Kit for Single-Cell Preparation","description":"\u003cp\u003e\u003cstrong\u003eFireGene Fallopian Tube Dissociation Kit\u003c\/strong\u003e is designed for enzymatic dissociation of mammalian fallopian tube tissues, including human and mouse samples. The kit supports preparation of single-cell suspensions with simple operation and stable performance. Prepared cells can be used for single-cell sequencing, cell sorting, cell culture, and related cellular experiments. The protocol uses a two-step digestion workflow with FTDS 1 and FTDS 2, followed by filtration, washing, resuspension, and quality control.\u003c\/p\u003e\n\u003ch2\u003eBackground Information\u003c\/h2\u003e\n\u003cp\u003eFallopian tube tissue contains epithelial, stromal, immune, and vascular components that can be difficult to dissociate consistently. A tissue-specific two-step digestion workflow helps prepare cell suspensions for reproductive biology and disease research.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Fallopian Tube Dissociation Kit for Single-Cell Preparation\u003c\/strong\u003e provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.\u003c\/p\u003e\n\u003ch3\u003eResearch Areas\u003c\/h3\u003e\n\u003cp\u003eThis product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eReproductive biology\u003c\/li\u003e\n\u003cli\u003eFallopian tube tissue research\u003c\/li\u003e\n\u003cli\u003eGynecological disease studies\u003c\/li\u003e\n\u003cli\u003eEpithelial cell biology\u003c\/li\u003e\n\u003cli\u003eCancer origin and tumor microenvironment research\u003c\/li\u003e\n\u003cli\u003eSingle-cell biology\u003c\/li\u003e\n\u003cli\u003eCell culture assay development\u003c\/li\u003e\n\u003cli\u003eTranslational biomedical research\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKey Applications\u003c\/h3\u003e\n\u003cp\u003eThe prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eFallopian tube single-cell suspension preparation\u003c\/li\u003e\n\u003cli\u003eSingle-cell sequencing\u003c\/li\u003e\n\u003cli\u003eFlow cytometry and cell sorting\u003c\/li\u003e\n\u003cli\u003ePrimary tissue cell culture\u003c\/li\u003e\n\u003cli\u003eCell viability and counting\u003c\/li\u003e\n\u003cli\u003eTissue heterogeneity analysis\u003c\/li\u003e\n\u003cli\u003eDisease mechanism research\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eFallopian Tube Dissociation Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBrand\u003c\/td\u003e\n\u003ctd\u003eFireGene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalog No.\u003c\/td\u003e\n\u003ctd\u003eFG-BA3336\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit Size\u003c\/td\u003e\n\u003ctd\u003e10 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Type\u003c\/td\u003e\n\u003ctd\u003eMammalian fallopian tube tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible Samples\u003c\/td\u003e\n\u003ctd\u003eHuman and mouse fallopian tube tissues\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended Starting Material\u003c\/td\u003e\n\u003ctd\u003eApproximately 200 mg fresh tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Function\u003c\/td\u003e\n\u003ctd\u003ePreparation of fallopian tube single-cell suspensions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWorkflow\u003c\/td\u003e\n\u003ctd\u003eTwo-step enzymatic digestion, filtration, centrifugation, washing, resuspension, quality control\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDownstream Applications\u003c\/td\u003e\n\u003ctd\u003eSingle-cell sequencing, cell sorting, cell culture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDigestion Conditions\u003c\/td\u003e\n\u003ctd\u003e37°C; 5–10 minutes with FTDS 1, then 15–45 minutes with FTDS 2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Instruments\u003c\/td\u003e\n\u003ctd\u003eWater bath or hybridization oven, horizontal centrifuge\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Reagents\u003c\/td\u003e\n\u003ctd\u003ePBS, FBS, RPMI 1640 medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Consumables\u003c\/td\u003e\n\u003ctd\u003eLow-adhesion pipette tips, 5 mL and 15 mL centrifuge tubes, 70 μm cell strainers\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOptional Related Reagent\u003c\/td\u003e\n\u003ctd\u003eRed Blood Cell Lysis Solution, Catalog No. BA3311\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf Life\u003c\/td\u003e\n\u003ctd\u003eTwo years\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eResearch Use\u003c\/td\u003e\n\u003ctd\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eCatalog Number\u003c\/th\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eFTDS 1 (Fallopian Tube Dissociation Solution 1)\u003c\/td\u003e\n\u003ctd\u003eFG-BA3336-A\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e2 ×1.1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFTDS 2 (Fallopian Tube Dissociation Solution 2)\u003c\/td\u003e\n\u003ctd\u003eFG-BA3336-B\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e4 ×1.35 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"FireGene","offers":[{"title":"10 rxns","offer_id":47868495233236,"sku":"FG-BA3336-10","price":949.0,"currency_code":"USD","in_stock":true},{"title":"50 rxns","offer_id":47868495266004,"sku":"FG-BA3336-50","price":3419.0,"currency_code":"USD","in_stock":true}]},{"product_id":"muscle-dissociation-kit","title":"FireGene Muscle Dissociation Kit for Single-Cell Suspension Preparation","description":"\u003cp\u003e\u003cstrong\u003eFireGene Muscle Dissociation Kit\u003c\/strong\u003e is designed for the isolation and preparation of single-cell suspensions from muscle tissue. The kit uses enzyme-based digestion to gently and efficiently disrupt extracellular matrix and release cells. Prepared cell suspensions can be used for single-cell sequencing, cell culture, and other cell-related assays. The protocol uses approximately 200 mg of fresh tissue, digestion at 37°C, filtration through a 70 μm cell strainer, washing, resuspension, and quality control.\u003c\/p\u003e\n\u003ch2\u003eBackground Information\u003c\/h2\u003e\n\u003cp\u003eMuscle tissue is dense and fibrous, which can make dissociation challenging. Enzymatic digestion provides a practical approach for preparing muscle-derived cell suspensions for downstream single-cell and culture-based research.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Muscle Dissociation Kit for Single-Cell Suspension Preparation\u003c\/strong\u003e provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.\u003c\/p\u003e\n\u003ch3\u003eResearch Areas\u003c\/h3\u003e\n\u003cp\u003eThis product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSkeletal muscle biology\u003c\/li\u003e\n\u003cli\u003eMuscle development and regeneration\u003c\/li\u003e\n\u003cli\u003eNeuromuscular disease research\u003c\/li\u003e\n\u003cli\u003eMetabolic disease studies\u003c\/li\u003e\n\u003cli\u003eExercise physiology\u003c\/li\u003e\n\u003cli\u003eRegenerative medicine\u003c\/li\u003e\n\u003cli\u003eSingle-cell biology\u003c\/li\u003e\n\u003cli\u003eDrug discovery and screening\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKey Applications\u003c\/h3\u003e\n\u003cp\u003eThe prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eMuscle tissue single-cell suspension preparation\u003c\/li\u003e\n\u003cli\u003eSingle-cell sequencing\u003c\/li\u003e\n\u003cli\u003eCell culture\u003c\/li\u003e\n\u003cli\u003eCell viability and counting\u003c\/li\u003e\n\u003cli\u003eCell-based assays\u003c\/li\u003e\n\u003cli\u003eMuscle tissue heterogeneity analysis\u003c\/li\u003e\n\u003cli\u003eDisease model research\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eMuscle Dissociation Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBrand\u003c\/td\u003e\n\u003ctd\u003eFireGene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalog No.\u003c\/td\u003e\n\u003ctd\u003eFG-BA3355\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit Size\u003c\/td\u003e\n\u003ctd\u003e10 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Type\u003c\/td\u003e\n\u003ctd\u003eMuscle tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible Samples\u003c\/td\u003e\n\u003ctd\u003eFresh muscle tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended Starting Material\u003c\/td\u003e\n\u003ctd\u003eApproximately 200 mg tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Function\u003c\/td\u003e\n\u003ctd\u003ePreparation of muscle single-cell suspensions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWorkflow\u003c\/td\u003e\n\u003ctd\u003eEnzymatic digestion, filtration, centrifugation, washing, resuspension, quality control\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDownstream Applications\u003c\/td\u003e\n\u003ctd\u003eSingle-cell sequencing, cell culture, cell-related assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDigestion Conditions\u003c\/td\u003e\n\u003ctd\u003e37°C for 0.5–2 hours\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Instruments\u003c\/td\u003e\n\u003ctd\u003eWater bath or hybridization oven, horizontal centrifuge\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Reagents\u003c\/td\u003e\n\u003ctd\u003ePBS, FBS, RPMI 1640 culture medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Consumables\u003c\/td\u003e\n\u003ctd\u003eLow-adsorption pipette tips, 5 mL and 15 mL centrifuge tubes, 70 μm cell strainers\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOptional Related Reagent\u003c\/td\u003e\n\u003ctd\u003eRed Blood Cell Lysis Solution, Catalog No. BA3311\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf Life\u003c\/td\u003e\n\u003ctd\u003eTwo years\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eResearch Use\u003c\/td\u003e\n\u003ctd\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eCatalog Number\u003c\/th\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eMTDS (Muscle Tissue Dissociation Solution)\u003c\/td\u003e\n\u003ctd\u003eFG-BA3355-A\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e2 × 1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"FireGene","offers":[{"title":"10 rxns","offer_id":47868495331540,"sku":"FG-BA3355-10","price":389.0,"currency_code":"USD","in_stock":true},{"title":"50 rxns","offer_id":47868495364308,"sku":"FG-BA3355-50","price":829.0,"currency_code":"USD","in_stock":true}]},{"product_id":"annelid-dissociation-kit","title":"FireGene Annelid Dissociation Kit for Single-Cell Preparation","description":"\u003cp\u003e\u003cstrong\u003eFireGene Annelid Dissociation Kit\u003c\/strong\u003e is designed for preparation of single-cell suspensions from various annelid tissues, including earthworms, leeches, and sandworms. The kit uses mild enzymatic digestion to release cells from field-collected or laboratory-bred annelid samples. Prepared cell suspensions can be used for single-cell sequencing, cell culture, and other cell-related assays. The protocol includes tissue washing, mincing, enzymatic digestion, filtration, washing, resuspension, and quality control.\u003c\/p\u003e\n\u003ch2\u003eBackground Information\u003c\/h2\u003e\n\u003cp\u003eAnnelids are used in developmental biology, regeneration, environmental biology, and comparative cell research. Tissue dissociation workflows help prepare cell suspensions from diverse annelid samples for downstream cellular analysis.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Annelid Dissociation Kit for Single-Cell Preparation\u003c\/strong\u003e provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.\u003c\/p\u003e\n\u003ch3\u003eResearch Areas\u003c\/h3\u003e\n\u003cp\u003eThis product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eAnnelid biology\u003c\/li\u003e\n\u003cli\u003eComparative cell biology\u003c\/li\u003e\n\u003cli\u003eRegeneration research\u003c\/li\u003e\n\u003cli\u003eDevelopmental biology\u003c\/li\u003e\n\u003cli\u003eEnvironmental and toxicology studies\u003c\/li\u003e\n\u003cli\u003eInvertebrate model research\u003c\/li\u003e\n\u003cli\u003eSingle-cell biology\u003c\/li\u003e\n\u003cli\u003eCell culture assay development\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKey Applications\u003c\/h3\u003e\n\u003cp\u003eThe prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eAnnelid tissue single-cell suspension preparation\u003c\/li\u003e\n\u003cli\u003eSingle-cell sequencing\u003c\/li\u003e\n\u003cli\u003eCell culture\u003c\/li\u003e\n\u003cli\u003eCell viability and counting\u003c\/li\u003e\n\u003cli\u003eCell-based assays\u003c\/li\u003e\n\u003cli\u003eTissue heterogeneity studies\u003c\/li\u003e\n\u003cli\u003eComparative organism research\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eAnnelid Dissociation Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBrand\u003c\/td\u003e\n\u003ctd\u003eFireGene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalog No.\u003c\/td\u003e\n\u003ctd\u003eFG-BA3335\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit Size\u003c\/td\u003e\n\u003ctd\u003e10 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Type\u003c\/td\u003e\n\u003ctd\u003eAnnelid tissues\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible Samples\u003c\/td\u003e\n\u003ctd\u003eFresh tissues from annelids, including earthworms, leeches, and sandworms\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended Starting Material\u003c\/td\u003e\n\u003ctd\u003eApproximately 200 mg tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Function\u003c\/td\u003e\n\u003ctd\u003ePreparation of annelid single-cell suspensions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWorkflow\u003c\/td\u003e\n\u003ctd\u003eEnzymatic digestion, filtration, centrifugation, washing, resuspension, quality control\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDownstream Applications\u003c\/td\u003e\n\u003ctd\u003eSingle-cell sequencing, cell culture, cell-related assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDigestion Conditions\u003c\/td\u003e\n\u003ctd\u003e37°C for 0.5–2 hours\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Instruments\u003c\/td\u003e\n\u003ctd\u003eWater bath or hybridization oven, horizontal centrifuge\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Reagents\u003c\/td\u003e\n\u003ctd\u003ePBS, FBS, RPMI 1640 medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Consumables\u003c\/td\u003e\n\u003ctd\u003eLow-adhesion pipette tips, 5 mL and 15 mL centrifuge tubes, 70 μm cell strainers\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOptional Related Reagent\u003c\/td\u003e\n\u003ctd\u003eRed Blood Cell Lysis Solution, Catalog No. BA3311\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf Life\u003c\/td\u003e\n\u003ctd\u003eTwo years\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eResearch Use\u003c\/td\u003e\n\u003ctd\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eCatalog Number\u003c\/th\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eADS (Annelid Dissociation Solution)\u003c\/td\u003e\n\u003ctd\u003eFG-BA3335-A\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e2 × 1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"FireGene","offers":[{"title":"10 rxns","offer_id":47868495397076,"sku":"FG-BA3335-10","price":789.0,"currency_code":"USD","in_stock":true},{"title":"50 rxns","offer_id":47868495429844,"sku":"FG-BA3335-50","price":3089.0,"currency_code":"USD","in_stock":true}]},{"product_id":"moth-intestinal-dissociation-kit","title":"FireGene Moth Intestinal Dissociation Kit for Single-Cell Preparation","description":"\u003cp\u003e\u003cstrong\u003eFireGene Moth Intestinal Dissociation Kit\u003c\/strong\u003e is designed for the isolation and preparation of cell suspensions from moth intestinal tissues. The kit uses enzymatic digestion to gently and effectively break down extracellular matrix and release cells. Prepared cell suspensions can be used for cell sorting, cell culture, and other cell-related assays. The workflow is optimized for insect tissue handling, with gentle pipetting and wide-bore tips recommended because insect cells are relatively fragile.\u003c\/p\u003e\n\u003ch2\u003eBackground Information\u003c\/h2\u003e\n\u003cp\u003eMoth intestinal tissue is relevant to insect physiology, host-pathogen interaction, microbiome, and pest biology research. Because insect cells can be fragile, gentle processing conditions are important during dissociation and resuspension.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Moth Intestinal Dissociation Kit for Single-Cell Preparation\u003c\/strong\u003e provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.\u003c\/p\u003e\n\u003ch3\u003eResearch Areas\u003c\/h3\u003e\n\u003cp\u003eThis product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eInsect biology\u003c\/li\u003e\n\u003cli\u003eMoth intestinal physiology\u003c\/li\u003e\n\u003cli\u003eHost-pathogen interaction studies\u003c\/li\u003e\n\u003cli\u003eInsect microbiome research\u003c\/li\u003e\n\u003cli\u003ePest biology and toxicology\u003c\/li\u003e\n\u003cli\u003eCell culture assay development\u003c\/li\u003e\n\u003cli\u003eCell sorting workflows\u003c\/li\u003e\n\u003cli\u003eComparative cell biology\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKey Applications\u003c\/h3\u003e\n\u003cp\u003eThe prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eMoth intestinal cell suspension preparation\u003c\/li\u003e\n\u003cli\u003eCell sorting\u003c\/li\u003e\n\u003cli\u003eCell culture\u003c\/li\u003e\n\u003cli\u003eCell viability and counting\u003c\/li\u003e\n\u003cli\u003eInsect intestinal tissue analysis\u003c\/li\u003e\n\u003cli\u003eCell-based assays\u003c\/li\u003e\n\u003cli\u003eTissue-specific insect research\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eMoth Intestinal Dissociation Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBrand\u003c\/td\u003e\n\u003ctd\u003eFireGene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalog No.\u003c\/td\u003e\n\u003ctd\u003eFG-BA3347\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit Size\u003c\/td\u003e\n\u003ctd\u003e10 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Type\u003c\/td\u003e\n\u003ctd\u003eMoth intestinal tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible Samples\u003c\/td\u003e\n\u003ctd\u003eFresh intestinal tissues from moths\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended Starting Material\u003c\/td\u003e\n\u003ctd\u003eIntestinal tissues from 10–15 fresh moths\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Function\u003c\/td\u003e\n\u003ctd\u003ePreparation of moth intestinal cell suspensions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWorkflow\u003c\/td\u003e\n\u003ctd\u003eEnzymatic digestion, filtration, centrifugation, optional wash, resuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDownstream Applications\u003c\/td\u003e\n\u003ctd\u003eCell sorting, cell culture, cell-related assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDigestion Conditions\u003c\/td\u003e\n\u003ctd\u003e28°C for 25–35 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Instruments\u003c\/td\u003e\n\u003ctd\u003eWater bath or hybridization oven, horizontal centrifuge\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Reagents\u003c\/td\u003e\n\u003ctd\u003eD-Hanks buffer, calcium and magnesium free; FBS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Consumables\u003c\/td\u003e\n\u003ctd\u003eLow-adhesion pipette tips, 5 mL and 15 mL centrifuge tubes, 40 μm cell strainers\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf Life\u003c\/td\u003e\n\u003ctd\u003eTwo years\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eResearch Use\u003c\/td\u003e\n\u003ctd\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eCatalog Number\u003c\/th\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eMIDS (Moth Intestinal Dissociation Solution)\u003c\/td\u003e\n\u003ctd\u003eFG-BA3347-A\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"FireGene","offers":[{"title":"10 rxns","offer_id":47868495462612,"sku":"FG-BA3347-10","price":539.0,"currency_code":"USD","in_stock":true},{"title":"50 rxns","offer_id":47868495495380,"sku":"FG-BA3347-50","price":1769.0,"currency_code":"USD","in_stock":true}]},{"product_id":"ovarian-dissociation-kit","title":"FireGene Ovarian Dissociation Kit for Single-Cell Preparation","description":"\u003cp\u003e\u003cstrong\u003eFireGene Ovarian Dissociation Kit\u003c\/strong\u003e is designed for enzymatic dissociation of ovarian tissues from large mammals. The kit addresses the challenge of difficult ovarian tissue dissociation and supports preparation of single-cell suspensions with high viability and low fragmentation rates. Prepared cells can be used for single-cell sequencing, cell sorting, cell culture, and related cellular experiments. The protocol uses a two-step digestion workflow with ODS 1 and ODS 2, followed by filtration, washing, and quality control.\u003c\/p\u003e\n\u003ch2\u003eBackground Information\u003c\/h2\u003e\n\u003cp\u003eOvarian tissue contains follicles, stromal cells, immune cells, vascular cells, and other specialized cell populations. Tissue-specific dissociation helps prepare single-cell suspensions for reproductive biology, ovarian disease models, and cellular analysis.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Ovarian Dissociation Kit for Single-Cell Preparation\u003c\/strong\u003e provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.\u003c\/p\u003e\n\u003ch3\u003eResearch Areas\u003c\/h3\u003e\n\u003cp\u003eThis product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eOvarian biology\u003c\/li\u003e\n\u003cli\u003eReproductive biology\u003c\/li\u003e\n\u003cli\u003eFemale reproductive disease research\u003c\/li\u003e\n\u003cli\u003eOvarian cancer and tumor microenvironment studies\u003c\/li\u003e\n\u003cli\u003eFertility and follicle research\u003c\/li\u003e\n\u003cli\u003eSingle-cell biology\u003c\/li\u003e\n\u003cli\u003eCell culture assay development\u003c\/li\u003e\n\u003cli\u003eTranslational biomedical research\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKey Applications\u003c\/h3\u003e\n\u003cp\u003eThe prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eLarge mammal ovarian single-cell suspension preparation\u003c\/li\u003e\n\u003cli\u003eSingle-cell sequencing\u003c\/li\u003e\n\u003cli\u003eFlow cytometry and cell sorting\u003c\/li\u003e\n\u003cli\u003ePrimary ovarian cell culture\u003c\/li\u003e\n\u003cli\u003eCell viability and counting\u003c\/li\u003e\n\u003cli\u003eTissue heterogeneity analysis\u003c\/li\u003e\n\u003cli\u003eDisease mechanism studies\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eOvarian Dissociation Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBrand\u003c\/td\u003e\n\u003ctd\u003eFireGene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalog No.\u003c\/td\u003e\n\u003ctd\u003eFG-BA3351\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit Size\u003c\/td\u003e\n\u003ctd\u003e10 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Type\u003c\/td\u003e\n\u003ctd\u003eOvarian tissue from large mammals\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible Samples\u003c\/td\u003e\n\u003ctd\u003eFresh ovarian tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended Starting Material\u003c\/td\u003e\n\u003ctd\u003eApproximately 200 mg tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Function\u003c\/td\u003e\n\u003ctd\u003ePreparation of ovarian single-cell suspensions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWorkflow\u003c\/td\u003e\n\u003ctd\u003eTwo-step enzymatic digestion, filtration, centrifugation, washing, resuspension, quality control\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDownstream Applications\u003c\/td\u003e\n\u003ctd\u003eSingle-cell sequencing, cell sorting, cell culture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDigestion Conditions\u003c\/td\u003e\n\u003ctd\u003e37°C; 15–20 minutes with ODS 1, then 30 minutes to 1 hour with ODS 2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Instruments\u003c\/td\u003e\n\u003ctd\u003eWater bath or hybridization oven, horizontal centrifuge\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Reagents\u003c\/td\u003e\n\u003ctd\u003ePBS, FBS, RPMI 1640 medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Consumables\u003c\/td\u003e\n\u003ctd\u003eLow-adhesion pipette tips, 5 mL and 15 mL centrifuge tubes, 70 μm cell strainers\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOptional Related Reagent\u003c\/td\u003e\n\u003ctd\u003eRed Blood Cell Lysis Solution, Catalog No. BA3311\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf Life\u003c\/td\u003e\n\u003ctd\u003eTwo years\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eResearch Use\u003c\/td\u003e\n\u003ctd\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eCatalog Number\u003c\/th\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eODS 1 (Ovarian Dissociation Solution 1)\u003c\/td\u003e\n\u003ctd\u003eFG-BA3351-A\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e2 ×1.1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eODS 2 (Ovarian Dissociation Solution 2)\u003c\/td\u003e\n\u003ctd\u003eFG-BA3351-B\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e2 ×1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"FireGene","offers":[{"title":"10 rxns","offer_id":47868495528148,"sku":"FG-BA3351-10","price":539.0,"currency_code":"USD","in_stock":true},{"title":"50 rxns","offer_id":47868495560916,"sku":"FG-BA3351-50","price":1769.0,"currency_code":"USD","in_stock":true}]},{"product_id":"small-mammal-ovarian-dissociation-kit","title":"Small Mammal Ovarian Dissociation Kit for scRNA-seq (Mouse, Rat)","description":"\u003cp\u003e\u003cstrong\u003eFireGene Small Mammal Ovarian Dissociation Kit\u003c\/strong\u003e is designed for the isolation and preparation of cell suspensions from ovarian tissues of small mammals. The kit uses enzymatic digestion to gently and effectively disrupt extracellular matrix and release cells. Prepared single-cell suspensions can be used for single-cell sequencing, cell culture, and other cell-related assays. The protocol includes digestion, filtration, washing, resuspension, and quality control, with digestion time adjusted according to tissue condition.\u003c\/p\u003e\n\u003ch2\u003eBackground Information\u003c\/h2\u003e\n\u003cp\u003eSmall mammal ovarian tissue is widely used in reproductive biology, developmental studies, fertility models, and disease research. A dedicated dissociation workflow supports preparation of cell suspensions for downstream single-cell and culture-based applications.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Small Mammal Ovarian Dissociation Kit for Single-Cell Preparation\u003c\/strong\u003e provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.\u003c\/p\u003e\n\u003ch3\u003eResearch Areas\u003c\/h3\u003e\n\u003cp\u003eThis product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSmall mammal reproductive biology\u003c\/li\u003e\n\u003cli\u003eOvarian biology\u003c\/li\u003e\n\u003cli\u003eFertility and follicle research\u003c\/li\u003e\n\u003cli\u003eDevelopmental biology\u003c\/li\u003e\n\u003cli\u003eEndocrine research\u003c\/li\u003e\n\u003cli\u003eSingle-cell biology\u003c\/li\u003e\n\u003cli\u003eCell culture assay development\u003c\/li\u003e\n\u003cli\u003ePreclinical disease models\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKey Applications\u003c\/h3\u003e\n\u003cp\u003eThe prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSmall mammal ovarian single-cell suspension preparation\u003c\/li\u003e\n\u003cli\u003eSingle-cell sequencing\u003c\/li\u003e\n\u003cli\u003eCell culture\u003c\/li\u003e\n\u003cli\u003eCell viability and counting\u003c\/li\u003e\n\u003cli\u003eCell-based assays\u003c\/li\u003e\n\u003cli\u003eTissue heterogeneity analysis\u003c\/li\u003e\n\u003cli\u003eReproductive disease model research\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eSmall Mammal Ovarian Dissociation Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBrand\u003c\/td\u003e\n\u003ctd\u003eFireGene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalog No.\u003c\/td\u003e\n\u003ctd\u003eFG-BA3352\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit Size\u003c\/td\u003e\n\u003ctd\u003e10 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Type\u003c\/td\u003e\n\u003ctd\u003eOvarian tissue from small mammals\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible Samples\u003c\/td\u003e\n\u003ctd\u003eFresh ovarian tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended Starting Material\u003c\/td\u003e\n\u003ctd\u003eApproximately 200 mg tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Function\u003c\/td\u003e\n\u003ctd\u003ePreparation of ovarian single-cell suspensions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWorkflow\u003c\/td\u003e\n\u003ctd\u003eEnzymatic digestion, filtration, centrifugation, washing, resuspension, quality control\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDownstream Applications\u003c\/td\u003e\n\u003ctd\u003eSingle-cell sequencing, cell culture, cell-related assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDigestion Conditions\u003c\/td\u003e\n\u003ctd\u003e37°C for 0.5–2 hours\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Instruments\u003c\/td\u003e\n\u003ctd\u003eWater bath or hybridization oven, horizontal centrifuge\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Reagents\u003c\/td\u003e\n\u003ctd\u003ePBS, FBS, RPMI 1640 medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Consumables\u003c\/td\u003e\n\u003ctd\u003eLow-adhesion pipette tips, 5 mL and 15 mL centrifuge tubes, 70 μm cell strainers\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOptional Related Reagent\u003c\/td\u003e\n\u003ctd\u003eRed Blood Cell Lysis Solution, Catalog No. BA3311\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf Life\u003c\/td\u003e\n\u003ctd\u003eTwo years\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eResearch Use\u003c\/td\u003e\n\u003ctd\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"width: 63.7184%;\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"width: 21.1191%;\"\u003eCatalog Number\u003c\/th\u003e\n\u003cth style=\"width: 14.4404%;\"\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 63.7184%;\"\u003eSMODS (Small Mammal Ovarian Dissociation Solution)\u003c\/td\u003e\n\u003ctd style=\"width: 21.1191%;\"\u003eFG-BA3352-A\u003c\/td\u003e\n\u003ctd align=\"right\" style=\"width: 14.4404%;\"\u003e2 × 1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProdut FAQ\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003cstrong\u003eQ: What small mammal species is this kit validated for?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003eA: The kit is optimized for mouse and rat ovarian tissue. It is also compatible with other small mammal species — digestion time should be adjusted based on tissue density and mass for non-validated species.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003cstrong\u003eQ: Can the kit be used with frozen ovarian tissue?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003eA: The kit is designed for fresh ovarian tissue. For frozen tissue, a nuclei isolation workflow (snRNA-seq) is recommended instead. Contact us for guidance on selecting the right protocol.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003cstrong\u003eQ: Is red blood cell removal required?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003eA: Optional. For highly vascularized samples or blood-contaminated preparations, the Red Blood Cell Lysis Solution (Cat. No. FG-BA3311) can be used as an add-on step after dissociation. It is not included in the kit but is available separately.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003cstrong\u003eQ: What downstream single-cell platforms is this kit compatible with?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003eA: Validated for 10x Genomics Chromium, BD Rhapsody, and Drop-seq. The single-cell suspension output meets the viability and concentration requirements for standard microfluidic capture workflows.\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003e\u003cstrong\u003eQ: Is bulk pricing available?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"font-claude-response-body break-words whitespace-normal leading-[1.7]\"\u003eA: Yes. The kit is available in 10-reaction and 50-reaction formats. For larger quantities or custom packaging, submit an inquiry at the form below or email \u003ca class=\"underline underline underline-offset-2 decoration-1 decoration-current\/40 hover:decoration-current focus:decoration-current\" href=\"mailto:service@firegene.com\"\u003eservice@firegene.com\u003c\/a\u003e.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"FireGene","offers":[{"title":"10 rxns","offer_id":47868495593684,"sku":"FG-BA3352-10","price":539.0,"currency_code":"USD","in_stock":true},{"title":"50 rxns","offer_id":47868495626452,"sku":"FG-BA3352-50","price":1769.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/files\/SmallMammalOvarianDissociationKit.png?v=1779786288"},{"product_id":"corneal-dissociation-kit","title":"FireGene Corneal Dissociation Kit for Single-Cell Preparation","description":"\u003cp\u003e\u003cstrong\u003eFireGene Corneal Dissociation Kit\u003c\/strong\u003e is designed for the isolation and preparation of cell suspensions from corneal tissue. The kit uses enzyme-based digestion to gently and rapidly disrupt extracellular matrix and release cells. Prepared cell suspensions can be used for single-cell sequencing, cell culture, and other cell-related assays. The protocol includes digestion, filtration through a 70 μm cell strainer, washing, resuspension, optional 20 μm filtration when clumps are present, and quality control.\u003c\/p\u003e\n\u003ch2\u003eBackground Information\u003c\/h2\u003e\n\u003cp\u003eCorneal tissue contains specialized epithelial, stromal, endothelial, and immune-associated cells. Preparing a cell suspension helps researchers analyze corneal cell populations, tissue responses, and disease-related cellular changes.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Corneal Dissociation Kit for Single-Cell Preparation\u003c\/strong\u003e provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.\u003c\/p\u003e\n\u003ch3\u003eResearch Areas\u003c\/h3\u003e\n\u003cp\u003eThis product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eOcular biology\u003c\/li\u003e\n\u003cli\u003eCorneal tissue research\u003c\/li\u003e\n\u003cli\u003eEye disease models\u003c\/li\u003e\n\u003cli\u003eEpithelial and stromal cell biology\u003c\/li\u003e\n\u003cli\u003eInflammation and wound healing studies\u003c\/li\u003e\n\u003cli\u003eRegenerative medicine\u003c\/li\u003e\n\u003cli\u003eSingle-cell biology\u003c\/li\u003e\n\u003cli\u003eCell culture assay development\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKey Applications\u003c\/h3\u003e\n\u003cp\u003eThe prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eCorneal cell suspension preparation\u003c\/li\u003e\n\u003cli\u003eSingle-cell sequencing\u003c\/li\u003e\n\u003cli\u003eCell culture\u003c\/li\u003e\n\u003cli\u003eCell viability and counting\u003c\/li\u003e\n\u003cli\u003eCell-based assays\u003c\/li\u003e\n\u003cli\u003eCorneal tissue heterogeneity analysis\u003c\/li\u003e\n\u003cli\u003eOcular disease mechanism research\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eCorneal Dissociation Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBrand\u003c\/td\u003e\n\u003ctd\u003eFireGene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalog No.\u003c\/td\u003e\n\u003ctd\u003eFG-BA3357\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit Size\u003c\/td\u003e\n\u003ctd\u003e10 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Type\u003c\/td\u003e\n\u003ctd\u003eCorneal tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible Samples\u003c\/td\u003e\n\u003ctd\u003eFresh corneal tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended Starting Material\u003c\/td\u003e\n\u003ctd\u003eApproximately 200 mg tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Function\u003c\/td\u003e\n\u003ctd\u003ePreparation of corneal cell suspensions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWorkflow\u003c\/td\u003e\n\u003ctd\u003eEnzymatic digestion, filtration, centrifugation, washing, optional clump filtration, resuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDownstream Applications\u003c\/td\u003e\n\u003ctd\u003eSingle-cell sequencing, cell culture, cell-related assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDigestion Conditions\u003c\/td\u003e\n\u003ctd\u003e37°C for 20–30 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Instruments\u003c\/td\u003e\n\u003ctd\u003eWater bath or hybridization oven, horizontal centrifuge\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Reagents\u003c\/td\u003e\n\u003ctd\u003ePBS, FBS, RPMI 1640 culture medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Consumables\u003c\/td\u003e\n\u003ctd\u003eLow-adsorption pipette tips, centrifuge tubes, 20 μm and 70 μm cell strainers\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eBuffer A and Enzyme B: -20°C; Enzyme C: 4°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf Life\u003c\/td\u003e\n\u003ctd\u003eBuffer A and Enzyme B: 2 years; Enzyme C: 1 year\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eResearch Use\u003c\/td\u003e\n\u003ctd\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eCatalog Number\u003c\/th\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer A\u003c\/td\u003e\n\u003ctd\u003eFG-BA3357-A\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e21.6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEnzyme B\u003c\/td\u003e\n\u003ctd\u003eFG-BA3357-B\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e8 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEnzyme C\u003c\/td\u003e\n\u003ctd\u003eFG-BA3357-C\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e100 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"FireGene","offers":[{"title":"10 rxns","offer_id":47868495659220,"sku":"FG-BA3357-10","price":499.0,"currency_code":"USD","in_stock":true},{"title":"50 rxns","offer_id":47868495691988,"sku":"FG-BA3357-50","price":1679.0,"currency_code":"USD","in_stock":true}]}],"url":"https:\/\/firegene.com\/collections\/tissue-dissociation-kits-for-single-cell-sequencing.oembed","provider":"FireGene","version":"1.0","type":"link"}