{"product_id":"adipose-tissue-nuclei-isolation-kit","title":"FireGene Adipose Nuclei Isolation Kit for Single-Nucleus Sequencing","description":"\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e\u003cstrong\u003eFireGene Adipose Nuclei Isolation Kit \u003c\/strong\u003eis designed for the isolation and extraction of cell nuclei from human or mammalian adipose tissue samples. The kit uses surfactants to disrupt cell membranes and release nuclei while maintaining nuclear membrane integrity. Through tissue grinding, filtration, centrifugation, and density-layer separation, the workflow produces clean single-nucleus suspensions suitable for single-nucleus transcriptome sequencing, single-nucleus ATAC sequencing, and related nuclear analysis experiments.\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eBackground Information\u003c\/h2\u003e\n\u003cp data-end=\"1514\" data-start=\"1244\"\u003eAdipose tissue is lipid-rich and can be difficult to process into high-quality single-cell suspensions. Nuclei isolation provides a practical alternative for adipose samples, especially when working with frozen tissue or samples where whole-cell recovery is challenging.\u003c\/p\u003e\n\u003cp data-end=\"1681\" data-start=\"1516\"\u003e\u003cstrong data-end=\"1557\" data-start=\"1516\"\u003eFireGene Adipose Nuclei Isolation Kit\u003c\/strong\u003e supports preparation of clean single-nucleus suspensions for sequencing-based studies and other nuclear analysis workflows.\u003c\/p\u003e\n\u003ch3 data-end=\"1701\" data-start=\"1683\" data-section-id=\"15x6sgr\"\u003eResearch Areas\u003c\/h3\u003e\n\u003cp data-end=\"1819\" data-start=\"1703\"\u003eThis kit is suitable for research fields focused on adipose biology, metabolic regulation, and tissue heterogeneity.\u003c\/p\u003e\n\u003cul data-end=\"2160\" data-start=\"1821\"\u003e\n\u003cli data-end=\"1845\" data-start=\"1821\" data-section-id=\"j8jxsp\"\u003eAdipose tissue biology\u003c\/li\u003e\n\u003cli data-end=\"1874\" data-start=\"1846\" data-section-id=\"1dtts7f\"\u003eMetabolic disease research\u003c\/li\u003e\n\u003cli data-end=\"1893\" data-start=\"1875\" data-section-id=\"vni08e\"\u003eObesity research\u003c\/li\u003e\n\u003cli data-end=\"1935\" data-start=\"1894\" data-section-id=\"12xwy96\"\u003eDiabetes and insulin resistance studies\u003c\/li\u003e\n\u003cli data-end=\"1976\" data-start=\"1936\" data-section-id=\"uf81qv\"\u003eEndocrine and hormone-related research\u003c\/li\u003e\n\u003cli data-end=\"2017\" data-start=\"1977\" data-section-id=\"1xdu0b1\"\u003eInflammation and immune cell profiling\u003c\/li\u003e\n\u003cli data-end=\"2056\" data-start=\"2018\" data-section-id=\"1lp3159\"\u003eAging-related adipose tissue studies\u003c\/li\u003e\n\u003cli data-end=\"2080\" data-start=\"2057\" data-section-id=\"15qp2sv\"\u003eRegenerative medicine\u003c\/li\u003e\n\u003cli data-end=\"2124\" data-start=\"2081\" data-section-id=\"x5rqu8\"\u003eSingle-cell and single-nucleus multiomics\u003c\/li\u003e\n\u003cli data-end=\"2160\" data-start=\"2125\" data-section-id=\"10aof08\"\u003eTranslational biomedical research\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 data-end=\"2182\" data-start=\"2162\" data-section-id=\"1fuj4ew\"\u003eKey Applications\u003c\/h3\u003e\n\u003cp data-end=\"2290\" data-start=\"2184\"\u003eThe prepared nuclei suspensions can be used in downstream experiments that require clean nuclear material.\u003c\/p\u003e\n\u003cul data-end=\"2658\" data-start=\"2292\"\u003e\n\u003cli data-end=\"2337\" data-start=\"2292\" data-section-id=\"2haltg\"\u003eSingle-nucleus RNA sequencing, or snRNA-seq\u003c\/li\u003e\n\u003cli data-end=\"2385\" data-start=\"2338\" data-section-id=\"9vqous\"\u003eSingle-nucleus ATAC sequencing, or snATAC-seq\u003c\/li\u003e\n\u003cli data-end=\"2418\" data-start=\"2386\" data-section-id=\"1vdl0d5\"\u003eNuclear transcriptome analysis\u003c\/li\u003e\n\u003cli data-end=\"2454\" data-start=\"2419\" data-section-id=\"5n45lt\"\u003eChromatin accessibility profiling\u003c\/li\u003e\n\u003cli data-end=\"2505\" data-start=\"2455\" data-section-id=\"1wvz3b2\"\u003eCell-type composition analysis in adipose tissue\u003c\/li\u003e\n\u003cli data-end=\"2570\" data-start=\"2506\" data-section-id=\"j76kee\"\u003eGene expression profiling from fresh or frozen adipose samples\u003c\/li\u003e\n\u003cli data-end=\"2609\" data-start=\"2571\" data-section-id=\"bpmidh\"\u003eAdipose tissue heterogeneity studies\u003c\/li\u003e\n\u003cli data-end=\"2658\" data-start=\"2610\" data-section-id=\"1fqsuyz\"\u003eDisease mechanism research in metabolic models\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003eSpecifications\u003c\/h2\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eAdipose Nuclei Isolation Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalog No.\u003c\/td\u003e\n\u003ctd\u003eFG-BA3342\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit Size\u003c\/td\u003e\n\u003ctd\u003e10 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Type\u003c\/td\u003e\n\u003ctd\u003eHuman or mammalian adipose tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible Samples\u003c\/td\u003e\n\u003ctd\u003eCell samples, fresh tissue, and frozen tissue samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended Starting Material\u003c\/td\u003e\n\u003ctd\u003eApproximately 200 mg tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Function\u003c\/td\u003e\n\u003ctd\u003eIsolation and extraction of cell nuclei\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWorkflow\u003c\/td\u003e\n\u003ctd\u003eTissue mincing, grinding, lysis, filtration, centrifugation, density-layer separation, nuclei washing and resuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDownstream Applications\u003c\/td\u003e\n\u003ctd\u003eSingle-nucleus transcriptome sequencing, single-nucleus ATAC sequencing, related nuclear analysis experiments\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Instruments\u003c\/td\u003e\n\u003ctd\u003eHorizontal centrifuge, tissue grinder, cell counter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Tissue Tools\u003c\/td\u003e\n\u003ctd\u003eSurgical scissors, scalpels, forceps\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Reagents\u003c\/td\u003e\n\u003ctd\u003eRNase inhibitor for nuclear RNA-related experiments\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Consumables\u003c\/td\u003e\n\u003ctd\u003eGrinding tubes with zirconium beads, 5 mL and 15 mL centrifuge tubes, 40 μm cell strainers, optional 20 μm cell strainers\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProcessing Temperature\u003c\/td\u003e\n\u003ctd\u003eSubsequent procedures after Step 6 should be carried out on wet ice or ice blocks when possible\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eMain reagents: 4°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdditive Storage\u003c\/td\u003e\n\u003ctd\u003eAdditive Solution ① and Additive Solution ②: -20°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf Life\u003c\/td\u003e\n\u003ctd\u003eOne year\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eResearch Use\u003c\/td\u003e\n\u003ctd\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003eKit Components\u003c\/h2\u003e\n\u003ctable style=\"width: 100%; height: 176.344px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003cth style=\"width: 44.2689%; height: 19.5938px;\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"width: 32.5111%; height: 19.5938px;\"\u003eCatalog Number\u003c\/th\u003e\n\u003cth style=\"width: 19.2913%; height: 19.5938px;\" align=\"right\"\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 44.2689%; height: 19.5938px;\"\u003eLysis Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 32.5111%; height: 19.5938px;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3342-A\u003c\/td\u003e\n\u003ctd style=\"width: 19.2913%; height: 19.5938px;\" align=\"right\"\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 44.2689%; height: 19.5938px;\"\u003ePre-Suspension Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 32.5111%; height: 19.5938px;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3342-B\u003c\/td\u003e\n\u003ctd style=\"width: 19.2913%; height: 19.5938px;\" align=\"right\"\u003e15 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 44.2689%; height: 19.5938px;\"\u003ePost-Suspension Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 32.5111%; height: 19.5938px;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3342-C\u003c\/td\u003e\n\u003ctd style=\"width: 19.2913%; height: 19.5938px;\" align=\"right\"\u003e50 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 44.2689%; height: 19.5938px;\"\u003ePB 1\u003c\/td\u003e\n\u003ctd style=\"width: 32.5111%; height: 19.5938px;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3342-D\u003c\/td\u003e\n\u003ctd style=\"width: 19.2913%; height: 19.5938px;\" align=\"right\"\u003e3 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 44.2689%; height: 19.5938px;\"\u003ePB 2\u003c\/td\u003e\n\u003ctd style=\"width: 32.5111%; height: 19.5938px;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3342-E\u003c\/td\u003e\n\u003ctd style=\"width: 19.2913%; height: 19.5938px;\" align=\"right\"\u003e6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 44.2689%; height: 19.5938px;\"\u003ePB 3\u003c\/td\u003e\n\u003ctd style=\"width: 32.5111%; height: 19.5938px;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3342-F\u003c\/td\u003e\n\u003ctd style=\"width: 19.2913%; height: 19.5938px;\" align=\"right\"\u003e6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 44.2689%; height: 19.5938px;\"\u003eAdditive Solution ①\u003c\/td\u003e\n\u003ctd style=\"width: 32.5111%; height: 19.5938px;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3342-G\u003c\/td\u003e\n\u003ctd style=\"width: 19.2913%; height: 19.5938px;\" align=\"right\"\u003e4 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 44.2689%; height: 19.5938px;\"\u003eAdditive Solution ②\u003c\/td\u003e\n\u003ctd style=\"width: 32.5111%; height: 19.5938px;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3342-H\u003c\/td\u003e\n\u003ctd style=\"width: 19.2913%; height: 19.5938px;\" align=\"right\"\u003e150 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"FireGene","offers":[{"title":"10 rxns","offer_id":47868401320148,"sku":"FG-BA3342-10","price":699.0,"currency_code":"USD","in_stock":true},{"title":"50 rxns","offer_id":47868401352916,"sku":"FG-BA3342-50","price":2429.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/firegene.com\/products\/adipose-tissue-nuclei-isolation-kit","provider":"FireGene","version":"1.0","type":"link"}