{"product_id":"blood-vascular-dissociation-kit-plus","title":"FireGene Blood Vascular Dissociation Kit Plus for Single-Cell Preparation","description":"\u003cp\u003e\u003cstrong\u003eFireGene Blood Vascular Dissociation Kit Plus\u003c\/strong\u003e is an optimized enzyme-based kit for dissociation of blood vascular tissues from humans and other large mammals. The workflow is designed to improve dissociation efficiency while preserving cell viability, helping address the challenges of vascular tissue processing. Prepared cells can be used for single-cell sequencing, cell sorting, cell culture, and other cellular experiments. The protocol uses a two-step digestion workflow with quality checks during processing.\u003c\/p\u003e\n\u003ch2\u003eBackground Information\u003c\/h2\u003e\n\u003cp\u003eVascular tissues can be difficult to dissociate because of extracellular matrix structure and tissue-specific cellular organization. A vascular-focused dissociation workflow helps researchers prepare single-cell suspensions for studying endothelial cells, smooth muscle cells, immune cells, and other vascular cell populations.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Blood Vascular Dissociation Kit Plus for Single-Cell Preparation\u003c\/strong\u003e provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.\u003c\/p\u003e\n\u003ch3\u003eResearch Areas\u003c\/h3\u003e\n\u003cp\u003eThis product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eVascular biology\u003c\/li\u003e\n\u003cli\u003eCardiovascular disease research\u003c\/li\u003e\n\u003cli\u003eEndothelial cell biology\u003c\/li\u003e\n\u003cli\u003eSmooth muscle cell research\u003c\/li\u003e\n\u003cli\u003eAtherosclerosis and vascular inflammation\u003c\/li\u003e\n\u003cli\u003eAngiogenesis research\u003c\/li\u003e\n\u003cli\u003eTissue remodeling studies\u003c\/li\u003e\n\u003cli\u003eSingle-cell biology\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKey Applications\u003c\/h3\u003e\n\u003cp\u003eThe prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eVascular tissue single-cell suspension preparation\u003c\/li\u003e\n\u003cli\u003eSingle-cell sequencing\u003c\/li\u003e\n\u003cli\u003eFlow cytometry and cell sorting\u003c\/li\u003e\n\u003cli\u003ePrimary vascular cell culture\u003c\/li\u003e\n\u003cli\u003eCell viability and cell counting\u003c\/li\u003e\n\u003cli\u003eVascular tissue heterogeneity analysis\u003c\/li\u003e\n\u003cli\u003eCardiovascular disease model research\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eBlood Vascular Dissociation Kit (Plus)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBrand\u003c\/td\u003e\n\u003ctd\u003eFireGene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalog No.\u003c\/td\u003e\n\u003ctd\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3337\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit Size\u003c\/td\u003e\n\u003ctd\u003e10 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Type\u003c\/td\u003e\n\u003ctd\u003eBlood vascular tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible Samples\u003c\/td\u003e\n\u003ctd\u003eFresh vascular tissues from humans and other large mammals\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended Starting Material\u003c\/td\u003e\n\u003ctd\u003eApproximately 200 mg tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Function\u003c\/td\u003e\n\u003ctd\u003ePreparation of vascular tissue single-cell suspensions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWorkflow\u003c\/td\u003e\n\u003ctd\u003eTwo-step enzymatic digestion, dilution, filtration, centrifugation, washing, resuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDownstream Applications\u003c\/td\u003e\n\u003ctd\u003eSingle-cell sequencing, cell sorting, cell culture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDigestion Conditions\u003c\/td\u003e\n\u003ctd\u003e37°C; 20–30 minutes with BVDSP 1, then 30 minutes to 2 hours with BVDSP 2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Instruments\u003c\/td\u003e\n\u003ctd\u003eWater bath or hybridization oven, horizontal centrifuge\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Reagents\u003c\/td\u003e\n\u003ctd\u003ePBS, FBS, RPMI 1640 medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Consumables\u003c\/td\u003e\n\u003ctd\u003eLow-adhesion pipette tips, 5 mL and 15 mL centrifuge tubes, 70 μm cell strainers\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOptional Related Reagent\u003c\/td\u003e\n\u003ctd\u003eRed Blood Cell Lysis Solution, Catalog No. BA3311\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf Life\u003c\/td\u003e\n\u003ctd\u003eTwo years\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eResearch Use\u003c\/td\u003e\n\u003ctd\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eCatalog Number\u003c\/th\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eBVDSP 1 (Blood Vascular Dissociation Solution (Plus) 1)\u003c\/td\u003e\n\u003ctd\u003eFG-BA3337-A\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBVDSP 2 (Blood Vascular Dissociation Solution (Plus) 2)\u003c\/td\u003e\n\u003ctd\u003eFG-BA3337-A\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"FireGene","offers":[{"title":"10 rxns","offer_id":47868495036628,"sku":"FG-BA3337-10","price":2099.0,"currency_code":"USD","in_stock":true},{"title":"50 rxns","offer_id":47868495069396,"sku":"FG-BA3337-50","price":7999.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/firegene.com\/products\/blood-vascular-dissociation-kit-plus","provider":"FireGene","version":"1.0","type":"link"}