{"product_id":"dead-cell-removal-solution","title":"FireGene Dead Cell Removal Solution for Single-Cell Suspension Cleanup","description":"\u003cp\u003e\u003cstrong\u003eFireGene Dead Cell Removal Solution\u003c\/strong\u003e is designed for removing dead cells from single-cell suspensions derived from dissociated mammalian tissues. The workflow helps reduce dead cell contamination and improve sample background for downstream experiments such as flow cytometry analysis, single-cell sequencing, cell culture, and related cell-based assays. The protocol uses a layered separation workflow with DCRS, followed by centrifugation, collection of the upper layer, washing, resuspension, and quality control.\u003c\/p\u003e\n\u003ch2\u003eBackground Information\u003c\/h2\u003e\n\u003cp\u003eDead cells can increase background, affect flow cytometry results, reduce single-cell sequencing quality, and interfere with downstream cell culture. A cleanup step after tissue dissociation helps prepare samples for more reliable cell-based analysis.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Dead Cell Removal Solution for Single-Cell Suspension Cleanup\u003c\/strong\u003e provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.\u003c\/p\u003e\n\u003ch3\u003eResearch Areas\u003c\/h3\u003e\n\u003cp\u003eThis product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSingle-cell sample preparation\u003c\/li\u003e\n\u003cli\u003eFlow cytometry assay development\u003c\/li\u003e\n\u003cli\u003eMammalian tissue dissociation workflows\u003c\/li\u003e\n\u003cli\u003eCancer research\u003c\/li\u003e\n\u003cli\u003eImmunology\u003c\/li\u003e\n\u003cli\u003eCell culture research\u003c\/li\u003e\n\u003cli\u003eDrug discovery assays\u003c\/li\u003e\n\u003cli\u003eTranslational biomedical research\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKey Applications\u003c\/h3\u003e\n\u003cp\u003eThe prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDead cell removal from single-cell suspensions\u003c\/li\u003e\n\u003cli\u003eFlow cytometry analysis\u003c\/li\u003e\n\u003cli\u003eSingle-cell sequencing preparation\u003c\/li\u003e\n\u003cli\u003eCell culture workflows\u003c\/li\u003e\n\u003cli\u003eCell counting and quality control\u003c\/li\u003e\n\u003cli\u003ePost-dissociation sample cleanup\u003c\/li\u003e\n\u003cli\u003eCell-based assays\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eWorkflow and Sample-Preparation Value\u003c\/h3\u003e\n\u003cp\u003eThe processed cell suspension is centrifuged and resuspended in RPMI 1640 medium containing 2% FBS, then mixed 1:1 with DCRS. The mixture is layered over DCRS and centrifuged at 4°C, 700 ×g for 20 minutes. The upper liquid is collected without disturbing the pellet, washed with PBS containing 5% FBS, and resuspended for downstream use.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eDead Cell Removal Solution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBrand\u003c\/td\u003e\n\u003ctd\u003eFireGene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalog No.\u003c\/td\u003e\n\u003ctd\u003eFG-BA3338\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit Size\u003c\/td\u003e\n\u003ctd\u003e10 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Type\u003c\/td\u003e\n\u003ctd\u003eSingle-cell suspensions from dissociated mammalian tissues\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Function\u003c\/td\u003e\n\u003ctd\u003eRemoval of dead cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWorkflow\u003c\/td\u003e\n\u003ctd\u003eCentrifugation, DCRS layering, separation, upper-layer collection, washing, resuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDownstream Applications\u003c\/td\u003e\n\u003ctd\u003eFlow cytometry analysis, single-cell sequencing, cell culture, cell-related assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSeparation Conditions\u003c\/td\u003e\n\u003ctd\u003e4°C, 700 ×g, 20 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Instruments\u003c\/td\u003e\n\u003ctd\u003eHorizontal centrifuge, cell counter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Reagents\u003c\/td\u003e\n\u003ctd\u003ePBS, FBS, RPMI 1640 medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Consumables\u003c\/td\u003e\n\u003ctd\u003eLow-adhesion pipette tips, centrifuge tubes, 20 μm cell strainers\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOptional Related Reagent\u003c\/td\u003e\n\u003ctd\u003eRed Blood Cell Lysis Solution, Catalog No. BA3311\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e4°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf Life\u003c\/td\u003e\n\u003ctd\u003eOne year\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eResearch Use\u003c\/td\u003e\n\u003ctd\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eCatalog Number\u003c\/th\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eDCRS (Dead Cell Removal Solution)\u003c\/td\u003e\n\u003ctd\u003eFG-BA3338\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"FireGene","offers":[{"title":"10 rxns","offer_id":47868495790292,"sku":"FG-BA3338-10","price":329.0,"currency_code":"USD","in_stock":true},{"title":"40 rxns","offer_id":47868495823060,"sku":"FG-BA3338-40","price":919.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/firegene.com\/products\/dead-cell-removal-solution","provider":"FireGene","version":"1.0","type":"link"}