{"product_id":"high-efficiency-endotoxin-removal-purification-resin","title":"High-Efficiency Endotoxin Removal Purification Resin","description":"\u003cp\u003e\u003cstrong\u003eProduct Introduction\u003c\/strong\u003e\u003cbr\u003eThe Endotoxin Removal Purification Resin is designed for the removal of endotoxins from biogenic protein-based products (including proteins, peptides, antibodies, polysaccharides, etc.). Its mechanism involves immobilizing modified Polymyxin B onto 4% agarose microspheres to achieve the specific removal of endotoxins. Through this purification process, the endotoxin levels in a sample can be reduced to as low as 0.1 EU\/mL, while maintaining a high sample recovery rate.\u003c\/p\u003e\n\u003cp\u003eFireGene High-Efficiency Endotoxin Removal Purification Resin is stored in 20% ethanol, with a gel-to-storage solution ratio of 1:1; the product specifications listed correspond to the actual volume of the gel matrix.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Features\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"MsoTableGrid\" border=\"1\" cellspacing=\"0\" style=\"width: 99.9404%; height: 249.157px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 35.5938px;\"\u003e\n\u003ctd width=\"273\" valign=\"top\" style=\"width: 48.8372%; height: 35.5938px;\"\u003e\n\u003cp class=\"MsoNormal\" style=\"text-align: left;\"\u003e\u003cspan\u003eMatrix\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"432\" valign=\"top\" style=\"width: 77.2809%; height: 35.5938px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e4% Agarose Microspheres\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5938px;\"\u003e\n\u003ctd width=\"273\" valign=\"top\" style=\"width: 48.8372%; height: 35.5938px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eLigand\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"432\" valign=\"top\" style=\"width: 77.2809%; height: 35.5938px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eModified Polymyxin B\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5938px;\"\u003e\n\u003ctd width=\"273\" valign=\"top\" style=\"width: 48.8372%; height: 35.5938px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eBead size\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"432\" valign=\"top\" style=\"width: 77.2809%; height: 35.5938px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e45-165 µm\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5938px;\"\u003e\n\u003ctd width=\"273\" valign=\"top\" style=\"width: 48.8372%; height: 35.5938px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eCapacity\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"432\" valign=\"top\" style=\"width: 77.2809%; height: 35.5938px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u0026gt; 2,000,000 EU\/mL matrix\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5938px;\"\u003e\n\u003ctd width=\"273\" valign=\"top\" style=\"width: 48.8372%; height: 35.5938px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003ePressureMax\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"432\" valign=\"top\" style=\"width: 77.2809%; height: 35.5938px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e0.1 MPa, 1bar\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5938px;\"\u003e\n\u003ctd width=\"273\" valign=\"top\" style=\"width: 48.8372%; height: 35.5938px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003epH range\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"432\" valign=\"top\" style=\"width: 77.2809%; height: 35.5938px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e5-10\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5938px;\"\u003e\n\u003ctd width=\"273\" valign=\"top\" style=\"width: 48.8372%; height: 35.5938px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eBuffer\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"432\" valign=\"top\" style=\"width: 77.2809%; height: 35.5938px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e20% Ethanol\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eStorage Conditions\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at 2–8°C. Shelf life: 2 years.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProduct FAQ\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003e\u003cspan style=\"font-family: Calibri;\"\u003eQ: Why is the endotoxin removal efficiency low?\u003c\/span\u003e \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eA: This may be because the sample pH falls outside the optimal range for endotoxin binding; it is recommended to adjust the pH to 7\u003c\/span\u003e\u003cspan style=\"font-family: 宋体;\"\u003e–\u003c\/span\u003e\u003cspan style=\"font-family: Calibri;\"\u003e8 using 0.1 M NaOH or 0.1 M HCl.\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003e\u003cspan style=\"font-family: Calibri;\"\u003eQ: Why was the sample contaminated?\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eA: The resin had previously been used to purify other samples; do not use previously used resin to remove endotoxins from different samples.\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003e\u003cspan style=\"font-family: Calibri;\"\u003eQ: Why is the sample recovery rate low?\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eA: This may be due to non-specific adsorption of the sample onto the resin; it is recommended to increase the NaCl concentration in both the sample and the equilibration buffer.\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003e\u003cspan style=\"font-family: Calibri;\"\u003eQ: Which reagents can this product withstand?\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eA: 20% DMSO, 20% ethanol, 20% glycerol; 1 M urea, 300 mM imidazole; 0.05% Tween 20, 10 mM DTT, etc.\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003e\u003cspan style=\"font-family: Calibri;\"\u003eQ: How do I determine the required volume of packing material?\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eA: This can be determined based on the packing material's endotoxin binding capacity and the measured endotoxin content of your specific sample. If you are unable to measure the endotoxin content of your sample, for an initial experiment, you may proceed by loading the sample at a packing material-to-sample volume ratio of 1:1 or 1:2.\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003e\u003cspan style=\"font-family: Calibri;\"\u003eQ: Can your endotoxin removal and purification resin remove endotoxins from nucleic acid samples\u003c\/span\u003e\u003cspan style=\"font-family: 宋体;\"\u003e—\u003c\/span\u003e\u003cspan style=\"font-family: Calibri;\"\u003esuch as plasmids or DNA?\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eA: No, it cannot.\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003e\u003cspan style=\"font-family: Calibri;\"\u003eQ: Regarding this packing material for removing endotoxins from proteins, what are the typical recovery rates for the protein and removal rates for the endotoxin?\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eA: The protein recovery rate can reach 90%, and the endotoxin removal rate can reach 99%.\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003e\u003cspan style=\"font-family: Calibri;\"\u003eQ: Regarding the endotoxin removal packing material, the client wishes to confirm the appropriate flow rate. They are utilizing a column with an inner diameter of 3.5 cm and intend to pack it with 40 mL of the material; the sample consists of a protein solution that has already undergone filtration and column chromatography. They would like to confirm the maximum flow rate that can be applied.\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eA: To ensure a contact time of greater than 10 minutes during the column run, the maximum flow rate for 40 mL of packing material should not exceed 4 mL\/min.\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003e\u003cspan style=\"font-family: Calibri;\"\u003eQ: If not currently in use, can the endotoxin removal packing material be temporarily stored in 1% Triton X-114, or should it be stored in the recommended equilibration buffer?\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eA: How long do you mean by \"temporarily\"? Short-term storage\u003c\/span\u003e\u003cspan style=\"font-family: 宋体;\"\u003e—\u003c\/span\u003e\u003cspan style=\"font-family: Calibri;\"\u003eapproximately 30 minutes\u003c\/span\u003e\u003cspan style=\"font-family: 宋体;\"\u003e—\u003c\/span\u003e\u003cspan style=\"font-family: Calibri;\"\u003eis acceptable; however, if the material will not be used for an extended period, we recommend storing it in the equilibration buffer.\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003e\u003cspan style=\"font-family: Calibri;\"\u003eQ: What is the glycerol tolerance limit for the endotoxin removal purification resin?\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eA: We recommend using a concentration of no more than 5%; high concentrations of glycerol can increase viscosity, which may negatively impact sample recovery rates.\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003e\u003cspan style=\"font-family: Calibri;\"\u003eQ: Can HEPES buffer be used as the buffer solution for the endotoxin purification resin?\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eA: Yes, it can.\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003e\u003cspan style=\"font-family: Calibri;\"\u003eQ: Does the endotoxin removal packing material require regeneration prior to its first use?\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eA: Yes, it does.\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003e\u003cspan style=\"font-family: Calibri;\"\u003eQ: How can one ensure that the regeneration solution is endotoxin-free?\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eA: Use endotoxin-free water for preparing the reagents, subject the containers to endotoxin removal treatment, or select consumables that are certified to be endotoxin-free.\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"$419\/10mL","offer_id":47870978851028,"sku":null,"price":419.0,"currency_code":"USD","in_stock":true},{"title":"$2389\/100mL","offer_id":47870978883796,"sku":null,"price":2389.0,"currency_code":"USD","in_stock":true},{"title":"$5799\/500mL","offer_id":47870978916564,"sku":null,"price":5799.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/firegene.com\/products\/high-efficiency-endotoxin-removal-purification-resin","provider":"FireGene","version":"1.0","type":"link"}