{"product_id":"invitrogen™-superase-in™-rnase-inhibitor-20-u-μl","title":"Invitrogen™ SUPERase·In™ RNase Inhibitor (20 U\/μL)","description":"\u003ch3 id=\"qa_spec_label_includes_label\"\u003eIncludes\u003c\/h3\u003e\n\u003cp id=\"qa_spec_content_include_desc\"\u003eSupplied in a single tube\u003c\/p\u003e\n\u003cp id=\"qa_spec_content_teasertext\"\u003eBinds and inactivates a wide variety of RNases\u003c\/p\u003e\n\u003cdiv class=\"qa_spec_content_productfeatures\"\u003e\n\u003cp\u003eSUPERase·In™ RNase Inhibitor is a protein-based inhibitor of nonhuman origin that noncovalently binds and inhibits the most common and troublesome RNases, including RNase A, B, C, 1, and T1. SUPERase·In RNase Inhibitor can be used in any application where RNase contamination could be problematic. Because it inhibits a broader range of RNases than traditional RNase inhibitors, SUPERase·In is the most effective RNase inhibitor available, providing a higher level of protection against degradation. SUPERase·In does not interfere with other enzymes such as RNA polymerases, reverse transcriptase, or\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eTaq\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eDNA polymerase. Additionally, SUPERase In is active up to 65°C and over a pH range of 5.5 to 8.5.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eCompletely removes RNase contamination from glass and plastic surfaces\u003c\/li\u003e\n\u003cli\u003eExcels at removing high levels of RNase contamination where similar products fail\u003c\/li\u003e\n\u003cli\u003eProven effective at removing high concentrations of dried-on RNase A\u003c\/li\u003e\n\u003cli\u003eIdeal for cleaning work surfaces, pipettors, and equipment that must be RNase-free\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cb\u003eUnit definition\u003c\/b\u003e\u003cbr\u003eSUPERase·In RNase Inhibitor at 1U\/μL will block the degradation of 0.1μg\/μL labeled RNA by 2.5pg\/μL of RNase A, 2.5pg\/μL of RNase I, and 0.0075U\/μL of RNase T1, for four hours at 37°C, in 20mM Tris-HCl, pH 7.5, 50mM NaCl, 1mM EDTA. Analysis is by denaturing PAGE. SUPERase·In is currently the only ribonuclease inhibitor for which the unit activity is defined by such a functional assay.\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eIf using this product with standard antibody purification methods, please contact technical support to discuss experimental design.\u003c\/p\u003e\n\u003cp\u003eGene Expression Analysis and Genotyping, In Vitro Transcription, PCR and Real-Time PCR, Real Time PCR (qPCR), Reverse Transcription\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3 id=\"qa_spec_label_orderinfo_label\"\u003eOrder Info\u003c\/h3\u003e\n\u003cp id=\"qa_spec_content_orderinfo\"\u003eShipping Condition: Dry ice\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eSpecifications\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"collapsible_block collapsible_content\"\u003e\n\u003cdiv class=\"specification_section single_space\"\u003e\n\u003ctable class=\"spec_table\"\u003e\n\u003ctbody\u003e\n\u003ctr class=\"\"\u003e\n\u003ctd class=\"bold\"\u003eConcentration\u003c\/td\u003e\n\u003ctd\u003e50 mM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"\"\u003e\n\u003ctd class=\"bold\"\u003eContent And Storage\u003c\/td\u003e\n\u003ctd\u003eSupplied in a single tube that should be stored at -20°C.\u003cbr\u003e\u003cbr\u003eStorage buffer: 2 mM KH\u003csub\u003e2\u003c\/sub\u003ePO\u003csub\u003e4\u003c\/sub\u003e, 8 mM Na\u003csub\u003e2\u003c\/sub\u003eHPO\u003csub\u003e4\u003c\/sub\u003e,2.7 mM KCl, 137 mM NaCl, pH 7.4 in 50% glycerol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"\"\u003e\n\u003ctd class=\"bold\"\u003eProduct Line\u003c\/td\u003e\n\u003ctd\u003eAmbion, SUPERase•In\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"\"\u003e\n\u003ctd class=\"bold\"\u003eQuantity\u003c\/td\u003e\n\u003ctd\u003e2500 U\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"\"\u003e\n\u003ctd class=\"bold\"\u003eShipping Condition\u003c\/td\u003e\n\u003ctd\u003eDry Ice\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch2\u003e\u003cstrong\u003eF＆A\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eQ: What temperature and pH is SUPERase·In RNase Inhibitor (Cat. No. AM2696) active up to?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: SUPERase·In RNase Inhibitor (20 U\/µL) (Cat. No. AM2696) is active up to 65 degrees C and over a pH range of 5.5 to 8.5.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eQ: What kind of Rnases can be inhibited using SUPERase.In RNase Inhibitor?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: SUPERase.In RNase Inhibitor is a protein-based inhibitor of nonhuman origin that noncovalently binds and inhibits the most common and troublesome RNases, including RNase A, B, C, 1, and T1.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eQ: What storage buffer do you recommend for the SUPERase.In RNase Inhibitor?\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA: The storage buffer we recommend is as follows: 2 mM KH2PO4, 8 mM Na2HPO4, 2.7 mM KCl, 137 mM NaCl, pH 7.4 in 50% glycerol.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"$1264.00 \/ $454.00","offer_id":48366527906004,"sku":"FG-AM2696","price":1264.0,"currency_code":"USD","in_stock":false}],"url":"https:\/\/firegene.com\/products\/invitrogen%e2%84%a2-superase-in%e2%84%a2-rnase-inhibitor-20-u-%ce%bcl","provider":"FireGene","version":"1.0","type":"link"}