{"product_id":"muscle-nuclei-isolation-kit-single-nucleus-sequencing","title":"FireGene Muscle Nuclei Isolation Kit for Single-Nucleus Sequencing","description":"\u003cp\u003e\u003cstrong\u003eFireGene Muscle Nuclei Isolation Kit\u003c\/strong\u003e is designed for the isolation and extraction of nuclei from human or mammalian muscle tissue, including fresh and frozen samples. The kit uses surfactants to disrupt cell membranes and release nuclei while maintaining nuclear membrane integrity. Through tissue grinding, filtration, centrifugation, and density-layer separation, the workflow produces clean single-nucleus suspensions suitable for single-nucleus transcriptome sequencing, single-nucleus ATAC sequencing, and related nuclear analysis experiments.\u003c\/p\u003e\n\u003ch2\u003eBackground Information\u003c\/h2\u003e\n\u003cp data-start=\"1264\" data-end=\"1549\"\u003eMuscle tissue is dense and fibrous, which can make whole-cell dissociation challenging. Nuclei isolation offers a practical sample-preparation approach for muscle research, especially when working with frozen tissue or samples that are difficult to dissociate into intact viable cells.\u003c\/p\u003e\n\u003cp data-start=\"1551\" data-end=\"1736\"\u003eThis kit supports nuclei extraction from both \u003cstrong data-start=\"1597\" data-end=\"1631\"\u003efresh and frozen muscle tissue\u003c\/strong\u003e, helping researchers prepare single-nucleus suspensions for sequencing and molecular analysis workflows.\u003c\/p\u003e\n\u003ch3 data-section-id=\"15x6sgr\" data-start=\"1738\" data-end=\"1756\"\u003eResearch Areas\u003c\/h3\u003e\n\u003cp data-start=\"1758\" data-end=\"1888\"\u003eThe kit is suitable for research fields involving muscle structure, cellular diversity, gene regulation, and tissue heterogeneity.\u003c\/p\u003e\n\u003cul data-start=\"1890\" data-end=\"2179\"\u003e\n\u003cli data-section-id=\"14n1dst\" data-start=\"1890\" data-end=\"1915\"\u003eSkeletal muscle biology\u003c\/li\u003e\n\u003cli data-section-id=\"inffk0\" data-start=\"1916\" data-end=\"1953\"\u003eMuscle development and regeneration\u003c\/li\u003e\n\u003cli data-section-id=\"bj9e1o\" data-start=\"1954\" data-end=\"1986\"\u003eNeuromuscular disease research\u003c\/li\u003e\n\u003cli data-section-id=\"56mux0\" data-start=\"1987\" data-end=\"2017\"\u003eAging-related muscle studies\u003c\/li\u003e\n\u003cli data-section-id=\"fvk3gu\" data-start=\"2018\" data-end=\"2044\"\u003eMetabolic disease models\u003c\/li\u003e\n\u003cli data-section-id=\"9wj5ta\" data-start=\"2045\" data-end=\"2075\"\u003eExercise physiology research\u003c\/li\u003e\n\u003cli data-section-id=\"15qp2sv\" data-start=\"2076\" data-end=\"2099\"\u003eRegenerative medicine\u003c\/li\u003e\n\u003cli data-section-id=\"10aof08\" data-start=\"2100\" data-end=\"2135\"\u003eTranslational biomedical research\u003c\/li\u003e\n\u003cli data-section-id=\"x5rqu8\" data-start=\"2136\" data-end=\"2179\"\u003eSingle-cell and single-nucleus multiomics\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 data-section-id=\"1fuj4ew\" data-start=\"2181\" data-end=\"2201\"\u003eKey Applications\u003c\/h3\u003e\n\u003cp data-start=\"2203\" data-end=\"2309\"\u003eThe prepared nuclei suspensions can be used in downstream experiments that require clean nuclear material.\u003c\/p\u003e\n\u003cul data-start=\"2311\" data-end=\"2620\"\u003e\n\u003cli data-section-id=\"2haltg\" data-start=\"2311\" data-end=\"2356\"\u003eSingle-nucleus RNA sequencing, or snRNA-seq\u003c\/li\u003e\n\u003cli data-section-id=\"9vqous\" data-start=\"2357\" data-end=\"2404\"\u003eSingle-nucleus ATAC sequencing, or snATAC-seq\u003c\/li\u003e\n\u003cli data-section-id=\"1vdl0d5\" data-start=\"2405\" data-end=\"2437\"\u003eNuclear transcriptome analysis\u003c\/li\u003e\n\u003cli data-section-id=\"cxmgau\" data-start=\"2438\" data-end=\"2471\"\u003eChromatin accessibility studies\u003c\/li\u003e\n\u003cli data-section-id=\"oe9er5\" data-start=\"2472\" data-end=\"2504\"\u003eCell-type composition analysis\u003c\/li\u003e\n\u003cli data-section-id=\"14lu26y\" data-start=\"2505\" data-end=\"2567\"\u003eGene expression profiling from fresh or frozen muscle tissue\u003c\/li\u003e\n\u003cli data-section-id=\"kgsayk\" data-start=\"2568\" data-end=\"2620\"\u003eTissue heterogeneity and disease mechanism studies\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003eSpecifications\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"width: 26.7148%;\"\u003eSpecification\u003c\/th\u003e\n\u003cth style=\"width: 72.9242%;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.7148%;\"\u003eProduct Name\u003c\/td\u003e\n\u003ctd style=\"width: 72.9242%;\"\u003eMuscle Nuclei Isolation Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.7148%;\"\u003eCatalog No.\u003c\/td\u003e\n\u003ctd style=\"width: 72.9242%;\"\u003eFG-BA3315\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.7148%;\"\u003eKit Size\u003c\/td\u003e\n\u003ctd style=\"width: 72.9242%;\"\u003e10 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.7148%;\"\u003eSample Type\u003c\/td\u003e\n\u003ctd style=\"width: 72.9242%;\"\u003eHuman or mammalian muscle tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.7148%;\"\u003eCompatible Samples\u003c\/td\u003e\n\u003ctd style=\"width: 72.9242%;\"\u003eFresh and frozen muscle samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.7148%;\"\u003eRecommended Starting Material\u003c\/td\u003e\n\u003ctd style=\"width: 72.9242%;\"\u003eApproximately 200 mg tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.7148%;\"\u003eMain Function\u003c\/td\u003e\n\u003ctd style=\"width: 72.9242%;\"\u003eIsolation and extraction of cell nuclei\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.7148%;\"\u003eWorkflow\u003c\/td\u003e\n\u003ctd style=\"width: 72.9242%;\"\u003eTissue grinding, lysis, filtration, centrifugation, density-layer separation, nuclei resuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.7148%;\"\u003eDownstream Applications\u003c\/td\u003e\n\u003ctd style=\"width: 72.9242%;\"\u003eSingle-nucleus transcriptome sequencing, single-nucleus ATAC sequencing, related nuclear analysis experiments\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.7148%;\"\u003eRequired Instruments\u003c\/td\u003e\n\u003ctd style=\"width: 72.9242%;\"\u003eHorizontal centrifuge, tissue grinder, cell counter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.7148%;\"\u003eRequired Tissue Tools\u003c\/td\u003e\n\u003ctd style=\"width: 72.9242%;\"\u003eSurgical scissors, scalpels, forceps\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.7148%;\"\u003eRequired Consumables\u003c\/td\u003e\n\u003ctd style=\"width: 72.9242%;\"\u003eGrinding tubes with zirconium beads, 5 mL and 15 mL centrifuge tubes, 40 μm cell strainers, optional 20 μm cell strainers\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.7148%;\"\u003eAdditional Reagent\u003c\/td\u003e\n\u003ctd style=\"width: 72.9242%;\"\u003eRNase inhibitor for nuclear RNA-related experiments\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.7148%;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 72.9242%;\"\u003eMain reagents: 4°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.7148%;\"\u003eAdditive Storage\u003c\/td\u003e\n\u003ctd style=\"width: 72.9242%;\"\u003eAdditive Solution ① and Additive Solution ②: -20°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.7148%;\"\u003eShelf Life\u003c\/td\u003e\n\u003ctd style=\"width: 72.9242%;\"\u003eOne year\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.7148%;\"\u003eResearch Use\u003c\/td\u003e\n\u003ctd style=\"width: 72.9242%;\"\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003eKit Components\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"width: 45.6679%;\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"width: 33.574%;\"\u003eCatalog Number\u003c\/th\u003e\n\u003cth align=\"right\" style=\"width: 19.8556%;\"\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 45.6679%;\"\u003eLysis Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 33.574%;\"\u003eFG-BA3315-A\u003c\/td\u003e\n\u003ctd align=\"right\" style=\"width: 19.8556%;\"\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 45.6679%;\"\u003ePre-Suspension Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 33.574%;\"\u003eFG-BA3315-B\u003c\/td\u003e\n\u003ctd align=\"right\" style=\"width: 19.8556%;\"\u003e15 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 45.6679%;\"\u003ePost-Suspension Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 33.574%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3315-C\u003c\/td\u003e\n\u003ctd align=\"right\" style=\"width: 19.8556%;\"\u003e50 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 45.6679%;\"\u003ePB 1\u003c\/td\u003e\n\u003ctd style=\"width: 33.574%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3315-D\u003c\/td\u003e\n\u003ctd align=\"right\" style=\"width: 19.8556%;\"\u003e3 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 45.6679%;\"\u003ePB 2\u003c\/td\u003e\n\u003ctd style=\"width: 33.574%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3315-E\u003c\/td\u003e\n\u003ctd align=\"right\" style=\"width: 19.8556%;\"\u003e6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 45.6679%;\"\u003ePB 3\u003c\/td\u003e\n\u003ctd style=\"width: 33.574%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3315-F\u003c\/td\u003e\n\u003ctd align=\"right\" style=\"width: 19.8556%;\"\u003e6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 45.6679%;\"\u003eAdditive Solution ①\u003c\/td\u003e\n\u003ctd style=\"width: 33.574%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3315-G\u003c\/td\u003e\n\u003ctd align=\"right\" style=\"width: 19.8556%;\"\u003e4 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 45.6679%;\"\u003eAdditive Solution ②\u003c\/td\u003e\n\u003ctd style=\"width: 33.574%;\"\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3315-H\u003c\/td\u003e\n\u003ctd align=\"right\" style=\"width: 19.8556%;\"\u003e150 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"FireGene","offers":[{"title":"10 rxns","offer_id":47868356886740,"sku":"FG-BA3315-10","price":699.0,"currency_code":"USD","in_stock":true},{"title":"50 rxns","offer_id":47868356919508,"sku":"FG-BA3315-50","price":2429.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/firegene.com\/products\/muscle-nuclei-isolation-kit-single-nucleus-sequencing","provider":"FireGene","version":"1.0","type":"link"}