{"product_id":"plant-protoplast-isolation-kit","title":"FireGene Universal Plant Protoplast Isolation Kit for Single-Cell Research","description":"\u003cp\u003e\u003cstrong\u003eFireGene Universal Plant Protoplast Isolation Kit\u003c\/strong\u003e is designed for the isolation and preparation of protoplast suspensions from higher plant tissues. The kit uses enzyme-based digestion to gently and effectively break down plant cell walls, releasing protoplasts into a single-protoplast suspension. The prepared protoplasts can be used for single-cell sequencing, tissue culture, protoplast fusion, and other plant cell research applications. The protocol includes enzymatic digestion, filtration, centrifugation, optional purification, and quality control by microscope or cell counter.\u003c\/p\u003e\n\u003ch2\u003eBackground Information\u003c\/h2\u003e\n\u003cp data-end=\"1628\" data-start=\"1261\"\u003ePlant protoplasts are cells with the cell wall removed, making them useful for experiments that require direct access to the plasma membrane, cytoplasm, or cellular contents. Because plant cell walls can limit access during many experimental workflows, enzymatic protoplast isolation is widely used in plant biology, functional genomics, and cell engineering studies.\u003c\/p\u003e\n\u003cp data-end=\"1878\" data-start=\"1630\"\u003e\u003cstrong data-end=\"1683\" data-start=\"1630\"\u003eFireGene Universal Plant Protoplast Isolation Kit\u003c\/strong\u003e provides a practical workflow for preparing protoplast suspensions from fresh, tender plant tissue while supporting downstream applications that require viable and clean protoplast preparations.\u003c\/p\u003e\n\u003ch3 data-end=\"1898\" data-start=\"1880\" data-section-id=\"15x6sgr\"\u003eResearch Areas\u003c\/h3\u003e\n\u003cp data-end=\"2033\" data-start=\"1900\"\u003eThis kit is suitable for plant research fields involving cellular function, gene regulation, tissue culture, and plant biotechnology.\u003c\/p\u003e\n\u003cul data-end=\"2305\" data-start=\"2035\"\u003e\n\u003cli data-end=\"2055\" data-start=\"2035\" data-section-id=\"1l8rfo0\"\u003ePlant cell biology\u003c\/li\u003e\n\u003cli data-end=\"2083\" data-start=\"2056\" data-section-id=\"623sjb\"\u003ePlant functional genomics\u003c\/li\u003e\n\u003cli data-end=\"2113\" data-start=\"2084\" data-section-id=\"90febs\"\u003ePlant developmental biology\u003c\/li\u003e\n\u003cli data-end=\"2132\" data-start=\"2114\" data-section-id=\"czq2rq\"\u003ePlant physiology\u003c\/li\u003e\n\u003cli data-end=\"2156\" data-start=\"2133\" data-section-id=\"hwqzqj\"\u003eCrop science research\u003c\/li\u003e\n\u003cli data-end=\"2188\" data-start=\"2157\" data-section-id=\"1vtq3ud\"\u003ePlant stress response studies\u003c\/li\u003e\n\u003cli data-end=\"2211\" data-start=\"2189\" data-section-id=\"1wmzkni\"\u003ePlant tissue culture\u003c\/li\u003e\n\u003cli data-end=\"2239\" data-start=\"2212\" data-section-id=\"1i77kch\"\u003ePlant genetic engineering\u003c\/li\u003e\n\u003cli data-end=\"2268\" data-start=\"2240\" data-section-id=\"ujtcy4\"\u003ePlant single-cell analysis\u003c\/li\u003e\n\u003cli data-end=\"2305\" data-start=\"2269\" data-section-id=\"1sl2mky\"\u003eProtoplast-based assay development\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 data-end=\"2327\" data-start=\"2307\" data-section-id=\"1fuj4ew\"\u003eKey Applications\u003c\/h3\u003e\n\u003cp data-end=\"2417\" data-start=\"2329\"\u003eThe prepared single-protoplast suspensions can be used in multiple downstream workflows.\u003c\/p\u003e\n\u003cul data-end=\"2695\" data-start=\"2419\"\u003e\n\u003cli data-end=\"2449\" data-start=\"2419\" data-section-id=\"ay04jo\"\u003ePlant single-cell sequencing\u003c\/li\u003e\n\u003cli data-end=\"2472\" data-start=\"2450\" data-section-id=\"1wmzkni\"\u003ePlant tissue culture\u003c\/li\u003e\n\u003cli data-end=\"2492\" data-start=\"2473\" data-section-id=\"tra2to\"\u003eProtoplast fusion\u003c\/li\u003e\n\u003cli data-end=\"2528\" data-start=\"2493\" data-section-id=\"yk40za\"\u003eTransient gene expression studies\u003c\/li\u003e\n\u003cli data-end=\"2565\" data-start=\"2529\" data-section-id=\"ogluht\"\u003ePlant cell transformation research\u003c\/li\u003e\n\u003cli data-end=\"2606\" data-start=\"2566\" data-section-id=\"ucfhzk\"\u003eCell viability and morphology analysis\u003c\/li\u003e\n\u003cli data-end=\"2650\" data-start=\"2607\" data-section-id=\"y9jbo2\"\u003ePlant cell-type and heterogeneity studies\u003c\/li\u003e\n\u003cli data-end=\"2695\" data-start=\"2651\" data-section-id=\"whc974\"\u003eCell-based screening and functional assays\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eUniversal Plant Protoplast Isolation Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalog No.\u003c\/td\u003e\n\u003ctd\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3317\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit Size\u003c\/td\u003e\n\u003ctd\u003e10 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Type\u003c\/td\u003e\n\u003ctd\u003eHigher plant tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended Sample\u003c\/td\u003e\n\u003ctd\u003eFresh, tender plant tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended Starting Material\u003c\/td\u003e\n\u003ctd\u003eApproximately 200–300 mg tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Function\u003c\/td\u003e\n\u003ctd\u003eIsolation and preparation of plant protoplast suspensions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWorkflow\u003c\/td\u003e\n\u003ctd\u003eMannitol pretreatment, enzymatic digestion, filtration, centrifugation, optional PPS purification, protoplast resuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDownstream Applications\u003c\/td\u003e\n\u003ctd\u003eSingle-cell sequencing, tissue culture, protoplast fusion, cell-related research\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Instruments\u003c\/td\u003e\n\u003ctd\u003eOrbital shaker, surgical scalpel, horizontal centrifuge\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Reagents\u003c\/td\u003e\n\u003ctd\u003e15% mannitol solution, BSA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Consumables\u003c\/td\u003e\n\u003ctd\u003eLow-adsorption pipette tips, 30 mL conical flasks, 15 mL centrifuge tubes, 70 μm cell strainers\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDigestion Conditions\u003c\/td\u003e\n\u003ctd\u003e3–5 hours at 25°C or room temperature, with microscopic monitoring\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eEnzyme A: -20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer Storage\u003c\/td\u003e\n\u003ctd\u003eBuffer B, PPS, and Buffer C: 4°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf Life\u003c\/td\u003e\n\u003ctd\u003eEnzyme A: 2 years; Buffer B, PPS, and Buffer C: 1 year\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eResearch Use\u003c\/td\u003e\n\u003ctd\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eCatalog Number\u003c\/th\u003e\n\u003cth align=\"right\"\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eEnzyme A\u003c\/td\u003e\n\u003ctd\u003eFG-BA3317-A\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e50 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer B\u003c\/td\u003e\n\u003ctd\u003eFG-BA3317-B\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e80 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePPS, Protoplast Purification Solution\u003c\/td\u003e\n\u003ctd\u003eFG-BA3317-C\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer C\u003c\/td\u003e\n\u003ctd\u003eFG-BA3317-G\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"FireGene","offers":[{"title":"10 rxns","offer_id":47868375400660,"sku":"FG-BA3317-10","price":889.0,"currency_code":"USD","in_stock":false},{"title":"50 rxns","offer_id":47868375433428,"sku":"FG-BA3317-50","price":3259.0,"currency_code":"USD","in_stock":false}],"url":"https:\/\/firegene.com\/products\/plant-protoplast-isolation-kit","provider":"FireGene","version":"1.0","type":"link"}