Overview
The FireGene Red Blood Cell Lysis Kit is formulated for the rapid and effective removal of red blood cells (RBCs) from single-cell suspensions. Widely applied in flow cytometry, immunological studies, and molecular biology, this kit improves the clarity and quality of samples used for downstream cell analysis and extraction workflows.
- Crucial for processing peripheral blood and lymphoid tissues.
- Enhances accuracy in:
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Flow cytometry, by isolating white blood cells for phenotypic analysis.
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Single-cell sequencing, by removing cellular noise caused by erythrocytes.
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Protein and nucleic acid extraction, by providing purified cellular material.
- Widely used in clinical diagnostics, immunological research, and biomarker discovery.
Detection Principle
- Combines osmotic shock and enzymatic digestion:
- The buffer induces a hypertonic environment that causes red blood cells to absorb water and rupture.
- Enzymes degrade RBC-specific membrane components, ensuring selective lysis.
- Result:
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White blood cells and other cell types remain intact, suitable for further analysis or functional assays.
Specifications
| Applications |
Single-cell sequencing, cell culture or other cell-related detections |
| Compatible Sample Types |
Single-cell suspension |
| Supported Instruments |
Water bath, horizontal centrifuge, cell counter |
| Storage |
4 °C |
| Shelf-life |
24 months |
Kit Components
| Component |
100 mL/Kit |
| RBCL |
100 mL |
