{"product_id":"serum-free-dmso-free-cell-freezing-medium","title":"FireGene Serum-Free and DMSO-Free Cell Freezing Medium for Cell Cryopreservation","description":"\u003cp\u003e\u003cstrong\u003eFireGene Serum-Free and DMSO-Free Cell Freezing Medium\u003c\/strong\u003e is designed for cryopreservation of animal primary cells and immortalized cell lines that are sensitive to animal-origin components or DMSO. The formulation is serum-free, animal-origin-free, DMSO-free, and non-toxic. Cells are resuspended in the freezing medium, aliquoted into cryovials, placed in a programmable freezing container for controlled-rate cooling at approximately 1°C\/min, and stored at -80°C before optional transfer to liquid nitrogen for longer-term storage.\u003c\/p\u003e\n\u003ch2\u003eBackground Information\u003c\/h2\u003e\n\u003cp data-end=\"1577\" data-start=\"1237\"\u003eDMSO is commonly used in cell freezing media, but some primary cells and sensitive cell lines may require alternatives that avoid DMSO and animal-origin components. A serum-free and DMSO-free freezing medium can help support cell banking workflows where a clearer culture background or reduced exposure to undefined components is preferred.\u003c\/p\u003e\n\u003cp data-end=\"1788\" data-start=\"1579\"\u003e\u003cstrong data-end=\"1637\" data-start=\"1579\"\u003eFireGene Serum-Free and DMSO-Free Cell Freezing Medium\u003c\/strong\u003e provides a defined cryopreservation option for animal primary cells and immortalized cell lines that are sensitive to DMSO or animal-origin materials.\u003c\/p\u003e\n\u003ch3 data-end=\"1808\" data-start=\"1790\" data-section-id=\"15x6sgr\"\u003eResearch Areas\u003c\/h3\u003e\n\u003cp data-end=\"1923\" data-start=\"1810\"\u003eThis freezing medium is suitable for cell culture and cryopreservation workflows across multiple research fields.\u003c\/p\u003e\n\u003cul data-end=\"2191\" data-start=\"1925\"\u003e\n\u003cli data-end=\"1939\" data-start=\"1925\" data-section-id=\"1wbfad3\"\u003eCell biology\u003c\/li\u003e\n\u003cli data-end=\"1963\" data-start=\"1940\" data-section-id=\"1x0kd5n\"\u003ePrimary cell research\u003c\/li\u003e\n\u003cli data-end=\"1976\" data-start=\"1964\" data-section-id=\"r3ap7s\"\u003eImmunology\u003c\/li\u003e\n\u003cli data-end=\"1994\" data-start=\"1977\" data-section-id=\"143grux\"\u003eCancer research\u003c\/li\u003e\n\u003cli data-end=\"2041\" data-start=\"1995\" data-section-id=\"stme50\"\u003eStem cell and regenerative medicine research\u003c\/li\u003e\n\u003cli data-end=\"2072\" data-start=\"2042\" data-section-id=\"1w8a9b5\"\u003eDrug discovery and screening\u003c\/li\u003e\n\u003cli data-end=\"2108\" data-start=\"2073\" data-section-id=\"10aof08\"\u003eTranslational biomedical research\u003c\/li\u003e\n\u003cli data-end=\"2143\" data-start=\"2109\" data-section-id=\"buxy7j\"\u003eCell banking and biopreservation\u003c\/li\u003e\n\u003cli data-end=\"2191\" data-start=\"2144\" data-section-id=\"18prr6a\"\u003eAssay development using sensitive cell models\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 data-end=\"2213\" data-start=\"2193\" data-section-id=\"1fuj4ew\"\u003eKey Applications\u003c\/h3\u003e\n\u003cp data-end=\"2340\" data-start=\"2215\"\u003eThe product supports routine and specialized cryopreservation workflows where serum-free and DMSO-free conditions are needed.\u003c\/p\u003e\n\u003cul data-end=\"2694\" data-start=\"2342\"\u003e\n\u003cli data-end=\"2384\" data-start=\"2342\" data-section-id=\"ljcpsh\"\u003eCryopreservation of animal primary cells\u003c\/li\u003e\n\u003cli data-end=\"2437\" data-start=\"2385\" data-section-id=\"16be5n0\"\u003eCryopreservation of immortalized animal cell lines\u003c\/li\u003e\n\u003cli data-end=\"2463\" data-start=\"2438\" data-section-id=\"7tf4a4\"\u003eDMSO-free cell freezing\u003c\/li\u003e\n\u003cli data-end=\"2489\" data-start=\"2464\" data-section-id=\"fwfv2l\"\u003eSerum-free cell banking\u003c\/li\u003e\n\u003cli data-end=\"2551\" data-start=\"2490\" data-section-id=\"1oiz1hk\"\u003ePreservation of cells sensitive to animal-origin components\u003c\/li\u003e\n\u003cli data-end=\"2592\" data-start=\"2552\" data-section-id=\"1xfdjj5\"\u003eCell recovery before culture expansion\u003c\/li\u003e\n\u003cli data-end=\"2646\" data-start=\"2593\" data-section-id=\"1yfj1k2\"\u003eLong-term storage after transfer to liquid nitrogen\u003c\/li\u003e\n\u003cli data-end=\"2694\" data-start=\"2647\" data-section-id=\"15lwr3b\"\u003eCell storage for downstream functional assays\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003eSpecifications\u003c\/h2\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eSerum-Free and DMSO-Free Cell Freezing Medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalog No.\u003c\/td\u003e\n\u003ctd\u003eFG-BA3341\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePack Size\u003c\/td\u003e\n\u003ctd\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFormulation Features\u003c\/td\u003e\n\u003ctd\u003eSerum-free, animal-origin-free, DMSO-free, non-toxic\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible Samples\u003c\/td\u003e\n\u003ctd\u003eAnimal primary cells and immortalized cell lines\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Function\u003c\/td\u003e\n\u003ctd\u003eCell cryopreservation and preservation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWorkflow\u003c\/td\u003e\n\u003ctd\u003eCell counting, centrifugation, resuspension in freezing medium, cryovial aliquoting, controlled-rate cooling, -80°C storage, optional liquid nitrogen transfer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eControlled-Rate Cooling\u003c\/td\u003e\n\u003ctd\u003eRecommended; approximately 1°C\/min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eGeneral Cryopreservation Density\u003c\/td\u003e\n\u003ctd\u003e1 × 10⁶ cells\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage After Freezing Cells\u003c\/td\u003e\n\u003ctd\u003e-80°C initially; transfer to liquid nitrogen after 24 hours for longer-term storage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eThawing Temperature\u003c\/td\u003e\n\u003ctd\u003e37°C water bath\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eExample Thawing Centrifugation\u003c\/td\u003e\n\u003ctd\u003e300 ×g for 5 minutes at 4°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Instruments for Freezing\u003c\/td\u003e\n\u003ctd\u003eHorizontal centrifuge, cell counter, -80°C freezer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Instruments for Thawing\u003c\/td\u003e\n\u003ctd\u003eHorizontal centrifuge, water bath\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Reagents for Thawing\u003c\/td\u003e\n\u003ctd\u003eRPMI 1640 medium or suitable culture medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Consumables\u003c\/td\u003e\n\u003ctd\u003eCell cryovials, programmable freezing container, low-adsorption pipette tips, centrifuge tubes, alcohol cotton balls\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Storage\u003c\/td\u003e\n\u003ctd\u003e-20°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf Life\u003c\/td\u003e\n\u003ctd\u003eOne year\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eResearch Use\u003c\/td\u003e\n\u003ctd\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003eKit Components\u003c\/h2\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eCatalog Number\u003c\/th\u003e\n\u003cth align=\"right\"\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eSerum-Free and DMSO-Free Cell Freezing Medium\u003c\/td\u003e\n\u003ctd\u003eFG-BA3341\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"FireGene","offers":[{"title":"100 mL","offer_id":47868397355220,"sku":"FG-BA3341-100","price":199.0,"currency_code":"USD","in_stock":true},{"title":"500 mL","offer_id":47868397387988,"sku":"FG-BA3341-500","price":389.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/firegene.com\/products\/serum-free-dmso-free-cell-freezing-medium","provider":"FireGene","version":"1.0","type":"link"}