{"product_id":"tricolor-prestained-protein-ladder","title":"Tricolor Prestained Protein Ladder","description":"\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eProduct Introduction\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Arial;\"\u003eFG-\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eWJ107 consists of 10 protein bands ranging from 10 kDa to 180 kDa (10, 15, 25, 35, 40, 55, 70, 100, 130, and 180 kDa). This range allows for monitoring protein separation during electrophoresis, estimating the molecular weight of target proteins, and assessing transfer efficiency in Western blotting. The 10 kDa and 70 kDa bands are conjugated with green or red dye, facilitating real-time monitoring of electrophoresis progress and molecular weight estimation.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eBuffer composition: 62.5 mM Tris-HCl pH7.5\/25°C\u003c\/span\u003e\u003cspan\u003e,\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003e, 1 mM EDTA, 2% (w\/v) SDS, 10 mM DTT, 33% (w\/v) glycerol.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eProduct Illustration\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cimg\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0634\/0912\/7636\/files\/1_4890e07a-15d4-4a6f-b372-54c98e55962b.png?v=1781081280\" alt=\"\"\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eOperating Steps\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e1. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eAllow the sample to stand at room temperature for several minutes before loading; after thawing, gently mix well to ensure the solution is homogeneous\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: 宋体;\"\u003e；\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e2. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eLoad an appropriate volume into the gel well; the recommended amount is 2–5 μL per well, which may be adjusted as necessary\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: 宋体;\"\u003e；\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e3. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eAfter use, close the tube cap and return it to 4°C or -20°C.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eNote: Based on a 5 μL sample, the protein content of each band in WJ107 is approximately 1 μg–3 μg.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003ePrecautions\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e1. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eThis product already contains loading buffer and is ready for direct use; do not heat or boil\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: 宋体;\"\u003e；\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e2. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eTo avoid repeated freeze-thaw cycles and contamination, aliquot the product and store at -20°C;\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e3. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003ePre-stained protein molecular weight standards are intended solely for approximate molecular weight reference; batch-to-batch variation is approximately 5%\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Arial;\"\u003e;\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e4. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eIn low-concentration gels, low-molecular-weight bands will co-migrate with the front and cannot be resolved;\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e5. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eThe migration rate of prestained proteins varies across different gel and buffer systems; this can be corrected using unstained protein molecular weight standards, allowing the proteins to be used normally based on the corrected results\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Arial;\"\u003e;\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e6. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eProtein molecular weight standards may undergo non-specific binding with antibodies; this can be optimized by reducing the antibody concentration, shortening the incubation time, and decreasing the loading amount of the protein molecular weight standards\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Arial;\"\u003e;\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e7. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eFor your safety and health, please wear a lab coat and disposable gloves while performing operations.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e8. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eThis product is for research use only.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":48081026777300,"sku":null,"price":0.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/firegene.com\/products\/tricolor-prestained-protein-ladder","provider":"FireGene","version":"1.0","type":"link"}