{"product_id":"tumor-dissociation-kit-plus","title":"FireGene Tumor Dissociation Kit Plus for Single-Cell Preparation","description":"\u003cp\u003e\u003cstrong\u003eFireGene Tumor Dissociation Kit Plus\u003c\/strong\u003e is designed for the isolation and preparation of single-cell suspensions from complex mammalian tumor tissues. The kit uses enzymes to gently and rapidly break down extracellular matrix and release cells. Prepared tumor cell suspensions can be used for single-cell sequencing, cell culture, and other cell-related assays. Digestion time can be adjusted based on species, tissue amount, and tumor condition, including samples with higher necrosis.\u003c\/p\u003e\n\u003ch2\u003eBackground Information\u003c\/h2\u003e\n\u003cp\u003eTumor tissues often contain diverse cell populations, dense extracellular matrix, stromal components, necrotic regions, and immune cell infiltration. A tumor-focused dissociation workflow helps prepare single-cell suspensions for studying tumor heterogeneity and the tumor microenvironment.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFireGene Tumor Dissociation Kit Plus for Single-Cell Preparation\u003c\/strong\u003e provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.\u003c\/p\u003e\n\u003ch3\u003eResearch Areas\u003c\/h3\u003e\n\u003cp\u003eThis product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eCancer biology\u003c\/li\u003e\n\u003cli\u003eTumor microenvironment research\u003c\/li\u003e\n\u003cli\u003eCancer immunology\u003c\/li\u003e\n\u003cli\u003eDrug discovery and screening\u003c\/li\u003e\n\u003cli\u003eTranslational oncology\u003c\/li\u003e\n\u003cli\u003eTumor heterogeneity studies\u003c\/li\u003e\n\u003cli\u003eSingle-cell biology\u003c\/li\u003e\n\u003cli\u003eCell culture assay development\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKey Applications\u003c\/h3\u003e\n\u003cp\u003eThe prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eTumor single-cell suspension preparation\u003c\/li\u003e\n\u003cli\u003eSingle-cell RNA sequencing\u003c\/li\u003e\n\u003cli\u003eFlow cytometry and cell sorting\u003c\/li\u003e\n\u003cli\u003ePrimary tumor cell culture\u003c\/li\u003e\n\u003cli\u003eCell viability and counting\u003c\/li\u003e\n\u003cli\u003eTumor microenvironment profiling\u003c\/li\u003e\n\u003cli\u003eDrug response and biomarker studies\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eTumor Dissociation Kit (Plus)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBrand\u003c\/td\u003e\n\u003ctd\u003eFireGene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalog No.\u003c\/td\u003e\n\u003ctd\u003eFG-BA3349\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit Size\u003c\/td\u003e\n\u003ctd\u003e10 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Type\u003c\/td\u003e\n\u003ctd\u003eComplex mammalian tumor tissues\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible Samples\u003c\/td\u003e\n\u003ctd\u003eFresh tumor tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended Starting Material\u003c\/td\u003e\n\u003ctd\u003eApproximately 200 mg tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Function\u003c\/td\u003e\n\u003ctd\u003ePreparation of tumor tissue single-cell suspensions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWorkflow\u003c\/td\u003e\n\u003ctd\u003eEnzymatic digestion, filtration, centrifugation, washing, resuspension, quality control\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDownstream Applications\u003c\/td\u003e\n\u003ctd\u003eSingle-cell sequencing, cell culture, cell-related assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDigestion Conditions\u003c\/td\u003e\n\u003ctd\u003e37°C for 10–40 minutes; longer for samples with high necrosis\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Instruments\u003c\/td\u003e\n\u003ctd\u003eWater bath or hybridization oven, horizontal centrifuge\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Reagents\u003c\/td\u003e\n\u003ctd\u003ePBS, FBS, RPMI 1640 medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Consumables\u003c\/td\u003e\n\u003ctd\u003eLow-adhesion pipette tips, 5 mL and 15 mL centrifuge tubes, 70 μm cell strainers\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOptional Related Reagent\u003c\/td\u003e\n\u003ctd\u003eRed Blood Cell Lysis Solution, Catalog No. BA3311\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf Life\u003c\/td\u003e\n\u003ctd\u003eTwo years\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eResearch Use\u003c\/td\u003e\n\u003ctd\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eCatalog Number\u003c\/th\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eTDSP (Tumor Dissociation Solution (Plus))\u003c\/td\u003e\n\u003ctd\u003eFG-BA3349\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"FireGene","offers":[{"title":"10 rxns","offer_id":47868495102164,"sku":"FG-BA3349-10","price":619.0,"currency_code":"USD","in_stock":true},{"title":"50 rxns","offer_id":47868495134932,"sku":"FG-BA3349-50","price":1769.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/firegene.com\/products\/tumor-dissociation-kit-plus","provider":"FireGene","version":"1.0","type":"link"}