{"product_id":"vascular-tissue-nuclei-isolation-kit","title":"FireGene Blood Vessel Nuclei Isolation Kit for Single-Nucleus Sequencing","description":"\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e\u003cstrong data-end=\"669\" data-start=\"623\"\u003eFireGene Blood Vessel Nuclei Isolation Kit\u003c\/strong\u003e is designed for the isolation and extraction of nuclei from cell suspensions, fresh tissues, and cryopreserved tissues derived from animal vascular tissues. The kit uses surfactants to disrupt cell membranes and release nuclei while maintaining nuclear membrane integrity. After tissue grinding, filtration, centrifugation, and density-layer separation, the workflow produces clean single-nucleus suspensions suitable for single-nucleus transcriptome sequencing, single-nucleus ATAC sequencing, and related nuclear analysis experiments.\u003c\/p\u003e\n\u003ch2\u003eBackground Information\u003c\/h2\u003e\n\u003cp data-end=\"1670\" data-start=\"1345\"\u003eBlood vessels contain multiple specialized cell populations, including endothelial cells, smooth muscle cells, pericytes, fibroblasts, and immune-associated cells. Preparing nuclei from vascular tissue can support molecular profiling when intact cell dissociation is challenging or when cryopreserved tissue samples are used.\u003c\/p\u003e\n\u003cp data-end=\"1861\" data-start=\"1672\"\u003e\u003cstrong data-end=\"1718\" data-start=\"1672\"\u003eFireGene Blood Vessel Nuclei Isolation Kit\u003c\/strong\u003e provides a practical workflow for preparing clean single-nucleus suspensions from animal vascular samples for sequencing and nuclear analysis.\u003c\/p\u003e\n\u003ch3 data-end=\"1881\" data-start=\"1863\" data-section-id=\"15x6sgr\"\u003eResearch Areas\u003c\/h3\u003e\n\u003cp data-end=\"2008\" data-start=\"1883\"\u003eThis kit is suitable for research fields focused on vascular structure, gene regulation, and tissue-level cellular diversity.\u003c\/p\u003e\n\u003cul data-end=\"2317\" data-start=\"2010\"\u003e\n\u003cli data-end=\"2028\" data-start=\"2010\" data-section-id=\"yufykc\"\u003eVascular biology\u003c\/li\u003e\n\u003cli data-end=\"2062\" data-start=\"2029\" data-section-id=\"1gha1eq\"\u003eCardiovascular disease research\u003c\/li\u003e\n\u003cli data-end=\"2090\" data-start=\"2063\" data-section-id=\"9jlppi\"\u003eEndothelial cell research\u003c\/li\u003e\n\u003cli data-end=\"2119\" data-start=\"2091\" data-section-id=\"1pzo0ic\"\u003eSmooth muscle cell biology\u003c\/li\u003e\n\u003cli data-end=\"2171\" data-start=\"2120\" data-section-id=\"1vdlgwu\"\u003eAtherosclerosis and vascular inflammation studies\u003c\/li\u003e\n\u003cli data-end=\"2195\" data-start=\"2172\" data-section-id=\"lxf1i7\"\u003eAngiogenesis research\u003c\/li\u003e\n\u003cli data-end=\"2237\" data-start=\"2196\" data-section-id=\"uwelti\"\u003eTissue remodeling and fibrosis research\u003c\/li\u003e\n\u003cli data-end=\"2281\" data-start=\"2238\" data-section-id=\"x5rqu8\"\u003eSingle-cell and single-nucleus multiomics\u003c\/li\u003e\n\u003cli data-end=\"2317\" data-start=\"2282\" data-section-id=\"10aof08\"\u003eTranslational biomedical research\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 data-end=\"2339\" data-start=\"2319\" data-section-id=\"1fuj4ew\"\u003eKey Applications\u003c\/h3\u003e\n\u003cp data-end=\"2442\" data-start=\"2341\"\u003eThe prepared nuclei suspensions can be used in downstream workflows requiring clean nuclear material.\u003c\/p\u003e\n\u003cul data-end=\"2825\" data-start=\"2444\"\u003e\n\u003cli data-end=\"2489\" data-start=\"2444\" data-section-id=\"2haltg\"\u003eSingle-nucleus RNA sequencing, or snRNA-seq\u003c\/li\u003e\n\u003cli data-end=\"2537\" data-start=\"2490\" data-section-id=\"9vqous\"\u003eSingle-nucleus ATAC sequencing, or snATAC-seq\u003c\/li\u003e\n\u003cli data-end=\"2570\" data-start=\"2538\" data-section-id=\"1vdl0d5\"\u003eNuclear transcriptome analysis\u003c\/li\u003e\n\u003cli data-end=\"2606\" data-start=\"2571\" data-section-id=\"5n45lt\"\u003eChromatin accessibility profiling\u003c\/li\u003e\n\u003cli data-end=\"2658\" data-start=\"2607\" data-section-id=\"10yh06\"\u003eCell-type composition analysis in vascular tissue\u003c\/li\u003e\n\u003cli data-end=\"2731\" data-start=\"2659\" data-section-id=\"1j6s7iv\"\u003eGene expression profiling from fresh or cryopreserved vascular samples\u003c\/li\u003e\n\u003cli data-end=\"2771\" data-start=\"2732\" data-section-id=\"12qwlyl\"\u003eVascular tissue heterogeneity studies\u003c\/li\u003e\n\u003cli data-end=\"2825\" data-start=\"2772\" data-section-id=\"1gnhqrm\"\u003eDisease mechanism research in cardiovascular models\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003eSpecifications\u003c\/h2\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eBlood Vessel Nuclei Isolation Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBrand\u003c\/td\u003e\n\u003ctd\u003eFireGene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit Size\u003c\/td\u003e\n\u003ctd\u003e10 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Source\u003c\/td\u003e\n\u003ctd\u003eAnimal vascular tissues\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible Samples\u003c\/td\u003e\n\u003ctd\u003eCell suspensions, fresh tissues, and cryopreserved tissues derived from animal vascular tissues\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended Starting Material\u003c\/td\u003e\n\u003ctd\u003eApproximately 200 mg tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Function\u003c\/td\u003e\n\u003ctd\u003eIsolation and extraction of nuclei\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWorkflow\u003c\/td\u003e\n\u003ctd\u003eLiquid nitrogen grinding, lysis, filtration, centrifugation, density-layer separation, nuclei washing and resuspension\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDownstream Applications\u003c\/td\u003e\n\u003ctd\u003eSingle-nucleus transcriptome sequencing, single-nucleus ATAC sequencing, related nuclear analysis experiments\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Instruments\u003c\/td\u003e\n\u003ctd\u003eHorizontal centrifuge, tissue grinder, cell counter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Tissue Tools\u003c\/td\u003e\n\u003ctd\u003eSurgical scissors, scalpels, forceps\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Reagents\u003c\/td\u003e\n\u003ctd\u003eRNase inhibitor for nuclear RNA-related experiments\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRequired Consumables\u003c\/td\u003e\n\u003ctd\u003eLiquid nitrogen, grinding tubes with zirconium beads, 5 mL and 15 mL centrifuge tubes, 40 μm cell strainers, optional 20 μm cell strainers\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProcessing Temperature\u003c\/td\u003e\n\u003ctd\u003ePost-lysis steps should be performed on wet ice or an ice block when possible\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eMain reagents: 4°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdditive Storage\u003c\/td\u003e\n\u003ctd\u003eAdditive Solution ① and Additive Solution ②: -20°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf Life\u003c\/td\u003e\n\u003ctd\u003eOne year\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eResearch Use\u003c\/td\u003e\n\u003ctd\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2\u003e\n\u003cmeta charset=\"utf-8\"\u003eKit Components\u003c\/h2\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eCatalog Number\u003c\/th\u003e\n\u003cth align=\"right\"\u003ePack Size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eLysis Buffer\u003c\/td\u003e\n\u003ctd\u003eFG-BA3343-A\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePre-Suspension Buffer\u003c\/td\u003e\n\u003ctd\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3343-B\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e15 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePost-Suspension Buffer\u003c\/td\u003e\n\u003ctd\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3343-C\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e50 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePB 1\u003c\/td\u003e\n\u003ctd\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3343-D\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e3 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePB 2\u003c\/td\u003e\n\u003ctd\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3343-E\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePB 3\u003c\/td\u003e\n\u003ctd\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3343-F\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdditive Solution ①\u003c\/td\u003e\n\u003ctd\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3343-G\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e4 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdditive Solution ②\u003c\/td\u003e\n\u003ctd\u003e\n\u003cmeta charset=\"utf-8\"\u003eFG-BA3343-H\u003c\/td\u003e\n\u003ctd align=\"right\"\u003e150 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"FireGene","offers":[{"title":"10 rxns","offer_id":47868399255764,"sku":"FG-BA3343-10","price":699.0,"currency_code":"USD","in_stock":true},{"title":"50 rxns","offer_id":47868399288532,"sku":"FG-BA3343-50","price":2429.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/firegene.com\/products\/vascular-tissue-nuclei-isolation-kit","provider":"FireGene","version":"1.0","type":"link"}