{"product_id":"western-blot-rapid-transfer-buffer10","title":"Western Blot Rapid Transfer Buffer(10×)","description":"\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eProduct Description\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eThis product utilizes a novel rapid transfer formulation, enabling the completion of Western Blot wet transfer in just 15–40 minutes. Anhydrous ethanol can be used instead of methanol during preparation; the process generates minimal heat, eliminating the need for an ice bath.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eProduct\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e \u003cspan style=\"font-family: Arial;\"\u003eFeatures\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan\u003eEfficient and convenient\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: 宋体;\"\u003e：\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Arial;\"\u003eWet transfer for Western blotting can be completed in 15\u003c\/span\u003e\u003cspan style=\"font-family: 宋体;\"\u003e–\u003c\/span\u003e\u003cspan style=\"font-family: Arial;\"\u003e40 minutes; heat generation is minimal, eliminating the need for an ice bath;\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Arial;\"\u003eOperational Safety\u003c\/span\u003e\u003cspan style=\"font-family: 宋体;\"\u003e：\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Arial;\"\u003eDuring preparation, anhydrous ethanol may be used instead of methanol to avoid the use of toxic reagents;\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan\u003eGood compatibility\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: 宋体;\"\u003e：\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Arial;\"\u003eSuitable for the transfer of various common gels.\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003ePreparation Method(1×)\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1000 mL Rapid Transfer Buffer (1×):\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e100 mL Rapid Transfer Buffer (10×) + 800 mL deionized water + 100 mL absolute ethanol (or methanol)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eNote: During preparation, first dilute the Rapid Transfer Buffer (10×) with deionized water, and add the alcohol last.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eReference conditions for membrane transfer\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eNote: This product requires a constant current of 400 mA for the membrane transfer process.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e1. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eGradient gel (4%–15%, 1.5 mm thick), three-color prestained molecular weight marker WJ103 (10–250 kDa); transfer performed at a constant current of 400 mA for 30 minutes, resulting in the transfer of all bands.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e2. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eFor standard PAGE gels, transfer efficiency depends on gel concentration. Using a constant current of 400 mA, proteins smaller than 150 kDa can be completely transferred from 1.0 mm gels of various concentrations within the durations listed below; for proteins larger than 150 kDa, the transfer time should be extended by 5–10 minutes:\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable class=\"MsoTableGrid\" border=\"1\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"261\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eGel concentration\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"153\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Arial;\"\u003e6%\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"150\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Arial;\"\u003e7.5%\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"145\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Arial;\"\u003e10%\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"142\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Arial;\"\u003e12.5%\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"261\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eRecommended duration\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"153\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Arial;\"\u003e15 min\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"150\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Arial;\"\u003e20 min\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"145\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Arial;\"\u003e30 min\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"142\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Arial;\"\u003e35 min\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e3. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eThe greater the PAGE gel thickness, the lower the transfer efficiency; for 1.5 mm thick gels, the transfer time specified in Item 2 can be extended by 5–10 minutes.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003ePrecautions\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e1. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eIf the transfer duration is 45 minutes or less, do not use an ice bath, as this will reduce transfer efficiency; if the duration exceeds 45 minutes, the temperature may become excessively high, so using an ice pack to lower the temperature is recommended to ensure optimal experimental results\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Arial;\"\u003e;\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e2. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eFor your safety and health, please wear a lab coat and disposable gloves while performing operations.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e3. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eThis product is for research use only.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"FireGene","offers":[{"title":"Default Title","offer_id":48084881277140,"sku":null,"price":0.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/firegene.com\/products\/western-blot-rapid-transfer-buffer10","provider":"FireGene","version":"1.0","type":"link"}