FireGene Blood Vessel Dissociation Kit for Single-Cell Applications

Overview

The FireGene Blood Vessel Dissociation Kit is specifically developed for the efficient enzymatic dissociation of vascular tissues into high-viability single-cell suspensions. This advanced kit is ideal for applications in single-cell sequencing, vascular biology, and drug discovery.

Background Information

• Driven by Clinical and Scientific Research Needs:

  • Understanding cellular heterogeneity in vascular tissues is vital for investigating vascular diseases such as atherosclerosis and vasculitis.
  • Conventional methods often damage delicate vascular cells or fail to isolate distinct populations.
  • This kit enables:
    • Identification of endothelial cells, smooth muscle cells, fibroblasts, and immune infiltrates.
    • Discovery of biomarkers and gene targets associated with vascular inflammation and remodeling.
    • Advancement of clinical research and therapeutic development in cardiovascular pathology.

• Background of Technological Development:

  • Designed to improve upon the low efficiency and high cell damage rates of traditional techniques.
  • Integrates:
    • Carefully selected enzymatic blends for vascular matrix degradation.
    • Optimized parameters for enzyme concentration, temperature, and incubation duration.
    • Workflow that ensures enhanced cell viability, higher recovery rates, and consistent performance across experiments.

Detection Principle

• Utilizes a multi-enzyme digestion protocol suited to the unique composition of blood vessel tissue.
• Process:
  • Tissue is finely sectioned to facilitate penetration.
  • Enzymes are applied sequentially under controlled temperature and timing.
  • Matrix components and cellular junctions are enzymatically degraded.
• Results in:
  • A clean, high-quality single-cell suspension ready for scRNA-seq, FACS, or downstream analysis.

Specifications

Kit Components