Product Overview
Description
The Kinetic Chromogenic Endotoxin Test Kit (TAL Assay) is a reliable and sensitive assay designed for the quantitative determination of bacterial endotoxin levels in human and animal injectable drugs, biological products, and medical devices.
Applications:
This assay is normally used for quantitative determination of bacterial endotoxin content in:
- Human and animal injection drugs
- Biological products
- Medical equipment
Composition:
- Limulus Amebocyte Lysate (LAL/TAL) Reagent
- Chromogenic Substrate
- Endotoxin Standard
- Control Standard Endotoxin (CSE)
- Standard and Quality Control Sample Diluent
- Reconstitution Buffer
- Microplates
Mechanism:
Bacterial endotoxin can specifically activate factor C in Limulus reagent, activated factor C activates factor B, activated factor B then activates procoagulase, coagulase hydrolyzes the chromogenic substrate in the reaction, and produces free pNA ( P-Nitroaniline) to cause absorbance changes, and the endotoxin concentration is quantified based on the kinetic detection solution absorbance change rate.
Specifications
| Methodology | Kinetic Chromogenic Method |
|
Time to Report |
~ 90 minutes |
| Used For | Injection drugs, Biological products, and Medical devices |
| Storage and Expiry | At 2-8°C the product can be stored for 24 months before opening. |
| Equipment & Consumables | Microplate reader, vortex shaker, pipette, Endotoxin-free tip are available to purchase. Tecan microplate reader is recommended. |