Product Overview
Background Information
Monkeypox is a viral zoonosis caused by the monkeypox virus that can be transmitted between animals and humans and can also be retransmitted between humans. Based on the current epidemiological investigation, the incubation period is 6 to 13 days, mostly 5 to 21 days. The main symptoms are fever, severe headache, swollen lymph nodes, back pain, muscle aches and weakness. The rash usually begins 1-3 days after a fever and is concentrated on the face and limbs, Lesions can occur at anywhere from a few to thousands of sites, and in severe cases they can combine to cause large chunks of skin to fall off. These rashes are often very painful. When a rash appears, the patient is contagious. The nucleic acid of Monkeypox infections can be detected via real-time PCR in the specimen of blood and throat swabs, papule or vesicle. The current kit includes a set of specific primers and probes pre-mixed in the Master Mix of the kit targeting two specific gene regions of Monkeypox Virus. The first gene is the orthopoxvirus DNA polymerase gene (E9L) for the detection of Eurasian orthopoxviruses other than variola viruses, and the second one is a viral envelope protein gene (B6R), which specifically detects monkeypox virus (MPXV). A set of primers and probes against human RNase P gene is also included as internal control (IC) for sample collection, processing, and detection.
Detection Principle
The detection of nucleic acid of Monkeypox Virus is based on quantitative real-time
PCR technology.In RT-PCR reaction, the forward and reverse primers bind to the specific regions of selected genes and amplify the regions (amplicons); the probes specifically bind to target sequence of the amplicons. Specific primers and probes are designed on the specific gene areas of Monkeypox Virus. Probes consist of a reporter fluorophore at 5' and quenching fluorophore at 3'. The fluorescent signals emitted from reporter fluorophores are absorbed by the quenchers, so it doesn’t emit signals. During amplification, probes bound to templates are cut off by Taq enzyme with 5'-3' exonuclease activity, separating reporter dye from the quencher, generating fluorescent signals, the PCR instrument will then automatically draw a real-time amplification curve based on the signal change, realizing the qualitative detection of Monkeypox Virus at the nucleic acid level.
Specifications
| Product Type | Molecular Detection |
| Applications | RT-PCR, MPV Nucleic Acids Detection |
| Compatible Sample Types | Blood, skin and throat swabs, papule or vesicle, etc. |
| Target | The orthopoxvirus DNA polymerase gene (E9L) and the viral envelope protein gene (B6R) |
| Method | Real-time fluorescent PCR |
| Prep Time | Results in 2 hours. |
| Analysis sensitivity | > 100 copies/ml |
| Supported Instruments | Ardent GS-PCR3200; ABI7500, ABI Quant Studio models 3/5/6/7/12K; Roche Lightcycler®480/1536/Nano; Agilent Mx3000P/3005P; Qiagen Rotor-Gene 6000/Q; Bio-Rad CFX384/CFX96, Bio-Rad Touch/iQ5; Cepheid Smart-cycler/Smart Cycler II; Eppendorf Master Cycler. |
| Storage | Room temp (lyophilized) |
| Shelf-life | 12 months |
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Kit Components
| 24 Tests/Kit | 48 Tests/Kit | 96 Tests/Kit | Main Compositions | |
| Master Mix (Lyophilized) | 3 8-tube strips | 6 8-tube strips | 12 8-tube strips | Tris-HCL; Primers; Probes; Enzyme mix including UDG; Taq polymerase; dNTPs; RNasin |
| Solvent | 400 μL | 1600 μL | 3200 μL | Tris-HCL; Glycerin; KCL; MgCl2 |
| Positive Control | 40 μL | 40 μL | 80 μL | Synthetic DNA Fragments; Tris-EDTA buffer |
| Nuclease-free Water | 0.5 mL | 0.5 mL | 0.5 mL | Nuclease-free Water |
Restrictions
- For In Vitro Diagnostic use only in EU regions.
- Research Use Only in the United States.
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