FireGene Bone Dissociation Kit – Enzymatic Single-Cell Prep

Overview

The FireGene Bone Dissociation Kit is a powerful enzymatic solution designed to generate high-quality single-cell suspensions from bone tissue. It is ideal for applications in bone disease research, regenerative medicine, and stem cell studies, offering consistent and viable cell isolation.

Background Information

• Driven by Clinical and Scientific Research Needs:

  • Bone tissue research is essential for studying diseases like osteoporosis and bone tumors.

  • Single-cell level analysis allows for better understanding of cellular heterogeneity in bone tissue.

  • Conventional tissue sectioning methods are insufficient for detailed cellular exploration.

  • In regenerative medicine, isolating viable bone cells (e.g., mesenchymal stem cells) is critical for tissue engineering.

  • High-quality single-cell suspensions support cell culture, differentiation, and downstream analysis.

• Background of Technological Development:

  • Traditional dissociation methods often lead to cell damage and poor efficiency.

  • This kit leverages advanced enzymatic techniques with:

    • Precisely selected enzymes tailored to bone matrix breakdown.

    • Optimized enzyme concentrations and reaction conditions.

    • Improved dissociation efficiency and enhanced cell viability for experimental reproducibility.

Detection Principle

• Uses a two-step enzymatic digestion method tailored to the density and complexity of bone tissue.

• Process:

  • Bone tissue is cut into small pieces.

  • Two enzyme mixtures are added successively under controlled conditions.

  • Enzymes break down the extracellular matrix and intercellular junctions.

• The result:

  • Clean, viable single-cell suspensions suitable for flow cytometry, scRNA-seq, and cell culture.

Specifications

Kit Components