DNA Polymerase I, Large (Klenow) Fragment

FireGeneSKU: FG-ENY104

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Product Overview

Description DNA Polymerase I, Large (Klenow) Fragment (about 68 kD) is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3'→5' exonuclease activity, but has lost 5'→3' exonuclease activity. Klenow retains the polymerization fidelity of the holoenzyme without degrading 5' termini. It is applicable to DNA sequencing by the Sanger dideoxy method, fill-in of 5' overhangs to form blunt ends, removal of 3' overhangs to form blunt ends, second strand cDNA synthesis and second strand synthesis in mutagenesis protocols. Theoretical protein molecular weight is 68000 daltons. Error Rate: ~ 18x10-6 bases
Source An E.coli strain that contains the E. coli polA gene that has had its 5'→3' exonuclease domain removed.
Applications Blunting, DNA Sequencing , Polymerases for DNA Manipulation, RT-qPCR, RT-PCR and cDNA Synthesis, RT-PCR & cDNA Synthesis, PCR
Size 200 U / 1000 U / 5000 U
Concentration 5,000 U/ml
Components DNA Polymerase I, Large (Klenow) Fragment (5,000 U/ml); 10X ABuffer B
Unit Definition One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C.
Usage For Research Use Only
Storage Storage condition: 25 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 @25°C. Store the DNA Polymerase I, Large (Klenow) Fragment at -20°C. Please avoid repeated freeze-thaw cycles.

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