The FireGene One-Step RT-LAMP SYBR Green kit is a reverse transcription loop-mediated isothermal amplification (RT-LAMP) based kit. The RT-LAMP Enzyme Mix contains the Bst DNA Polymerase and the M-MLV (H- ) Reverse Transcriptase (heat-resistant) as the key enzymes. It can be used for SYBR® Green I fluorescence quantification of LAMP with high sensitivity and strong specificity. Combined with the Buffer optimized for SYBR® Green I method, it can inhibit non-specific amplification, thereby significantly improving the amplification efficiency, and is suitable for high-sensitivity dye-based LAMP detection reactions. This product is provided in a convenient Mix, which can obtain a good standard curve in a wide range, and can accurately quantify and detect the target gene with high repeatability and reliability.
- Gene expression analysis of gene targets
|Component Name||Amount||Storage (°C)|
|RNase free ddH2O||1.25 ml x 4||-20|
|2 x LAMP SYBR Reaction Mixa||1.25 ml x 4||-20|
|RT LAMP Enzyme Mixb||125 μl x 4||-20|
a. Contains dNTPs, Mg2+ and SYBR® Green I
b. Contains Bst DNA Polymerase, M-MLV (H- ) Reverse Transcriptase (heat-resistant), RNasin, etc.
For Research or Educational Use Only.