The FireGene 1st strand cDNA Synthesis Kit contains all of the reagents necessary for synthesis of full-length first-strand cDNA from total or poly A+ RNA, including both oligo(dT) and random hexamers primers. This cDNA synthesis kit also works well with other cDNA-dependent techniques, including RT-PCR, cDNA probe preparation, quantitative PCR (qPCR), and PCR based cloning. The FireGene 1st strand cDNA Synthesis Kit is ideal for reverse transcription of many different RNA templates, including GC-rich templates and RNAs with high levels of secondary structure.
This kit includes 4 x gDNA wiper Mix that can completely remove residual genomic DNA contamination and ensure more reliable subsequent quantitative results, 2 x RT Mix that contains optimized buffer system and dNTP, and the Enzyme Mix contains FireGene Reverse Transcriptase and RNasin. You may also choose Oligo (dT)23VN, Random hexamers, or gene-specific primers as reverse transcription primers based on your requirements.
- First-strand cDNA synthesis
- cDNA probe preparation
- Synthesis of cDNA libraries with a high proportion of full-length cDNAs
|Component Name||Amount||Storage (°C)|
|RNase free ddH2O||1 ml x 2||-20|
|2 x RT Mix*1||1 ml||-20|
|Enzyme Mix*2||200 μl||-20|
|Oligo (dT)23VN (50 μM)||100 μl||-20|
|Random hexamers (50 ng/μl)||100 μl||-20|
|4 x gDNA wiper Mix||40 μl||-20|
*1. Each contains 1 mM dNTP
*2. Contains RNasin
For Research or Educational Use Only.