Product Overview
The FireGene 1st strand cDNA Synthesis Kit contains all of the reagents necessary for synthesis of full-length first-strand cDNA from total or poly A+ RNA, including both oligo(dT) and random hexamers primers. This cDNA synthesis kit also works well with other cDNA-dependent techniques, including RT-PCR, cDNA probe preparation, quantitative PCR (qPCR), and PCR based cloning. The FireGene 1st strand cDNA Synthesis Kit is ideal for reverse transcription of many different RNA templates, including GC-rich templates and RNAs with high levels of secondary structure.
This kit includes 4 x gDNA wiper Mix that can completely remove residual genomic DNA contamination and ensure more reliable subsequent quantitative results, 2 x RT Mix that contains optimized buffer system and dNTP, and the Enzyme Mix contains FireGene Reverse Transcriptase and RNasin. You may also choose Oligo (dT)23VN, Random hexamers, or gene-specific primers as reverse transcription primers based on your requirements.
Applications
- First-strand cDNA synthesis
- cDNA probe preparation
- RT-PCR
- Synthesis of cDNA libraries with a high proportion of full-length cDNAs
Components
| Component Name | Amount | Storage (°C) |
| RNase free ddH2O | 1 ml x 2 | -20 |
| 2 x RT Mix*1 | 1 ml | -20 |
| Enzyme Mix*2 | 200 μl | -20 |
| Oligo (dT)23VN (50 μM) | 100 μl | -20 |
| Random hexamers (50 ng/μl) | 100 μl | -20 |
| 4 x gDNA wiper Mix | 40 μl | -20 |
*1. Each contains 1 mM dNTP
*2. Contains RNasin
Restrictions
For Research or Educational Use Only.