The FireGene Lysate2Ct Kit provides a greatly simplified workflow - from cultured cell lysates directly to reverse transcription (RT) and real-time PCR, without RNA isolation. Cell lysates can be prepared in under 10 minutes at room temperature with a few simple steps of pipetting, and the process is compatible with high-throughput automated liquid handling workstations. Each lysis accommodates 10-5x105 cells, and the resulting lysates can be added to reverse transcription, and subsequently real-time PCR analysis. The performance characteristics, i.e. sensitivity and specificity, is comparable to that of the purified RNA approach.
Reverse transcription (RT), real-time PCR, gene expression, DNA quantitation, ChiP, etc.
|Compatible Sample Types
|Lysate, ready for real-time PCR
|Amount of Starting Material
|10 - 5x105 cells
|Compatible with high-throughput automated liquid handling workstations
|Number of Reactions
-20°C for all components except for the nuclease-free water
Room temperature for Nuclease-free Water
Note: After the 2xTaqMan Mix is thawed for the first time, it can be stable for up to 6 months when stored at 4°C.