Product Overview
Description
The FireGene Lysate2Ct Kit provides a greatly simplified workflow - from cultured cell lysates directly to reverse transcription (RT) and real-time PCR, without RNA isolation. Cell lysates can be prepared in under 10 minutes at room temperature with a few simple steps of pipetting, and the process is compatible with high-throughput automated liquid handling workstations. Each lysis accommodates 10-5x105 cells, and the resulting lysates can be added to reverse transcription, and subsequently real-time PCR analysis. The performance characteristics, i.e. sensitivity and specificity, is comparable to that of the purified RNA approach.
Specifications
| Product Type | RNA Extraction |
| Applications |
Reverse transcription (RT), real-time PCR, gene expression, DNA quantitation, ChiP, etc. |
| Compatible Sample Types | Cells |
| Target | Lysate, ready for real-time PCR |
| Method | Primer-Probe |
| Prep Time | 10 minutes |
| Amount of Starting Material | 10 - 5x105 cells |
| Supported Instruments | Compatible with high-throughput automated liquid handling workstations |
| Number of Reactions | 96 |
| Storage |
-20°C for all components except for the nuclease-free water Room temperature for Nuclease-free Water Note: After the 2xTaqMan Mix is thawed for the first time, it can be stable for up to 6 months when stored at 4°C. |
| Shelf-life | 12 months |
Components