Product Overview
Description
This kit utilized FireGene's original magnetic bead purification technology and a unique buffer system, which combines physical-mechanical and enzymatic digestion methods to fully lyse bacterial cell walls, especially for Gram-positive bacteria and other difficult-to-lyse bacteria. A large amount of nucleic acid is released, which doubles the yield of nucleic acid extraction, effectively avoids false negative results caused by insufficient wall breaking, and is suitable for extracting total DNA from various microorganisms. The extraction process does not involve chloroform, phenol and other reagents. The extracted genomic DNA has large fragments, high purity, stable and reliable quality, and can be used for downstream experiments such as enzyme digestion, library construction, cloning, PCR, quantitative PCR, chip detection, and high-throughput sequencing. The kit can also be efficiently used with automated liquid workstations and magnetic bar method magnetic bead automatic extraction systems for fast, high-throughput extraction.
Specifications
| Product Type | Nucleic Acid Extraction |
| Applications |
Enzyme digestion, library construction, cloning, PCR, quantitative PCR, chip detection, and high-throughput sequencing |
| Compatible Sample Types | Microbial samples |
| Target | DNA |
| Method | Bacterial wall breaking - digestion and lysis - magnetic beads binding nucleic acid - washing to remove impurities - elution to obtain high quality nucleic acid |
| Prep Time | |
| Amount of Starting Material | |
| Supported Instruments | Compatible with most automated nucleic acid extraction instruments and pipetting workstations |
| Number of Reactions | 48 |
| Storage |
-20°C for Proteinase K 2~8°C for Magnetic Bead Suspensions Room temperature for all other components |
| Shelf-life | 12 months |
Components