| Description |
HotStart Taq DNA polymerase is an innovative antibody-modified thermostable enzyme. The activity of the enzyme is completely closed at room temperature, avoiding the non-specific amplification and primer dimer in the preparation of the sample and the first cycle reaction temperature rise, increasing the specificity of DNA amplification. When heated to 95 °C, the antibody bound to the enzyme was rapidly inactivated and did not affect the subsequent Taq DNA polymerase reaction. The enzyme has the advantages of good specificity, high sensitivity, good reproducibility and high amplification efficiency. |
| Source |
An E.coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1. |
| Applications |
Multiplex PCR, PCR , Specialty PCR, Routine PCR |
| Size |
1000 U / 5000 U / 10000 U |
| Concentration |
5,000 U/ml |
| Components |
HotStart Taq DNA Polymerase (5,000 U/ml); 10X PCR Reaction Buffer, Mg2+ plus |
| Unit Definition |
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 75°C. |
| Usage |
For Research Use Only |
| Storage |
Storage Conditions: 10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% Tween 20, 0.5% NP-40, 50% Glycerol, pH 7.4 @ 25°C. Store the HotStart Taq DNA Polymerase (Mg2+ Plus Buffer) at -20°C. Please avoid repeated freeze-thaw cycles. |
