|Pfu DNA Polymerase is a high-fidelity, thermostable enzyme isolated from Pyrococcus furiosus. Pfu DNA Polymerase possesses 3'→5' exonuclease (proofreading) activity. Base misincorporations that may occur during polymerization are rapidly excised by this proofreading activity. MagicFast Pfu DNA Polymerase has been genetically engineered to enhance the amplification efficiency by fusing DNA binding domains to the Pfu DNA polymerase. MagicFast Pfu DNA Polymerase has the characteristics of high amplification speed (extension ~2 kb/min), high fidelity and strong anti-interference ability.
Consequently, MagicFast Pfu DNA Polymerase is recommended for use in PCR and primer extension reactions that require high-fidelity synthesis. MagicFast Pfu DNA Polymerase-generated PCR fragments are blunt-ended. MagicFast Pfu 2X PCR Master Mix is an optimized ready-to-use solution containing MagicFast Pfu DNA Polymerase, dNTPs, MgCl2, KCI and stabilizers, as well as two commonly used tracking dyes for DNA gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at ~4 kb and Tartrazine migrates at ~10 bp. Both dyes are present in concentrations that do not mask comigrating DNA bands.