| Description |
Pfu DNA Polymerase is a high-fidelity, thermostable enzyme isolated from Pyrococcus furiosus. Pfu DNA Polymerase possesses 3'→5' exonuclease (proofreading) activity. Base misincorporations that may occur during polymerization are rapidly excised by this proofreading activity. MagicFast Pfu DNA Polymerase has been genetically engineered to enhance the amplification efficiency by fusing DNA binding domains to the Pfu DNA polymerase. MagicFast Pfu DNA Polymerase has the characteristics of high amplification speed (extension ~2 kb/min), high fidelity and strong anti-interference ability. Consequently, MagicFast Pfu DNA Polymerase is recommended for use in PCR and primer extension reactions that require high-fidelity synthesis. MagicFast Pfu DNA Polymerase-generated PCR fragments are blunt-ended. |
| Source |
An E.coli strain that carries the Pfu DNA Polymerase gene from Pyrococcus furiosus. |
| Applications |
High Fidelity PCR, PCR , Routine PCR |
| Size |
1000 U / 5000 U |
| Concentration |
2,000 U/ml |
| Components |
MagicFast Pfu DNA Polymerase (2,000 U/ml); 10X PCR Reaction Buffer, Mg2+ plus |
| Unit Definition |
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 72°C. |
| Usage |
For Research Use Only |
| Storage |
Store the Pfu DNA Polymerase at -20°C. Please avoid repeated freeze-thaw cycles. |
