| Description |
MagicTaq DNA Polymerase is an optimized blend of Taq Klenow and Deep Oean DNA polymerases for use with routine and difficult PCR experiments. The 3'→ 5' exonuclease activity of Deep Ocean DNA Polymerase increases the fidelity and robust amplification of Taq klenow DNA Polymerase. The Magic Taq Reaction Buffers and High GC Enhancer have been formulated for robust yields with minimal optimization, regardless of a template's GC content. Magic Taq DNA Polymerase is supplied with two 5X buffers: (Standard and GC), as well as a High GC Enhancer solution. For most routine and/or AT-rich amplicons (Lambda, etc.) or complex amplicons with up to ~50% GC content, Magic Taq Standard Reaction Buffer provides robust amplification. For GC-rich amplicons, the First Taq GC Reaction Buffer can improve both performance and yield. For particularly high GC or difficult amplicons, the First Taq High GC Enhancer can be added at a final concentration of 10–20% to reactions containing FirstTaq GC Reaction Buffer. |
| Applications |
Multiplex PCR, PCR , Specialty PCR, Routine PCR |
| Size |
1000 U / 5000 U |
| Concentration |
5,000 U/ml |
| Components |
DNA Polymerase (5,000 U/ml); 5X GC Reaction Buffer; 5X Standard Reaction Buffer; 100% High GC Enhancer |
| Usage |
For Research Use Only |
| Storage |
Storage Conditions: 10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% Tween 20, 0.5% IGEPAL CA-630, 50% Glycerol, pH7.4 @ 25°C. Store DNA Polymerase at -20°C. Please avoid repeated freeze-thaw cycles. |
