FireGene Mouse Brain Dissociation Kit for scRNA-seq

Overview

The FireGene Mouse Brain Dissociation Kit is engineered to produce high-quality single-cell suspensions from mouse brain tissue using an optimized enzymatic process. This kit is ideal for neuroscience research, enabling precise gene expression profiling and functional studies at the single-cell level.

Background Information

• Driven by Clinical and Scientific Research Needs:

  • Increased demand for high-resolution studies of cellular heterogeneity in the brain.

  • Traditional methods fail to preserve delicate brain cell populations and often yield low viability.

  • Single-cell sequencing of mouse brain tissue enables:

    • Detailed gene expression analysis.

    • Functional characterization of neurons, glia, and other CNS cell types.

    • Discovery of new biomarkers and therapeutic targets for brain disorders.

  • Critical for exploring neurodevelopment, neurodegeneration, and brain repair mechanisms.

• Background of Technological Development:

  • Overcomes limitations of mechanical and chemical dissociation methods.

  • Employs advanced enzymatic protocols that:

    • Use optimized enzyme concentrations and incubation times.

    • Minimize damage to fragile neural cells.

    • Preserve cell viability and diversity.

  • Designed to ensure consistent, reproducible dissociation outcomes.

Detection Principle

• Utilizes a synergistic enzymatic digestion method optimized for brain tissue.

• Process highlights:

  • Mouse brain tissue is cut into small pieces for uniform exposure.

  • Sequential addition of enzyme mixtures facilitates gentle tissue breakdown.

  • Incubation is performed under controlled temperature and timing conditions.

  • Enzymes selectively degrade the extracellular matrix and cell junctions.

• Final output:

  • A clean, high-viability single-cell suspension ready for scRNA-seq, flow cytometry, and downstream analysis.

Specifications

Kit Components