Product Overview
Overview
FireGene’s Multi-Tissue Dissociation Kit is engineered for high-efficiency enzymatic tissue dissociation, ideal for generating high-quality single-cell suspensions from a variety of mammalian tissues. It supports applications in single-cell sequencing, disease modeling, and advanced drug development.
Background Information
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Designed to support single-cell research, disease mechanism studies, and therapeutic screening.
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Essential in tumor biology, where understanding cellular heterogeneity is key.
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Overcomes limitations of:
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Mechanical dissociation, which may damage cells.
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Chemical methods, which risk introducing toxic substances.
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Developed with deep insight into extracellular matrix-cell interactions.
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Combines multiple enzymes and a carefully optimized buffer system to:
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Adapt to the diverse structural profiles of different tissues.
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Ensure consistency and high yield across various experimental workflows.
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Provides researchers with a universal solution for tissue dissociation without compromising cell viability or function.
Detection Principle
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Utilizes a gentle enzymatic digestion process.
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Efficiently breaks down the extracellular matrix while preserving cell integrity.
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Supports:
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Rapid tissue processing
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Minimal cellular stress
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High-viability single-cell suspensions for downstream applications
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Compatible with scRNA-seq, flow cytometry, and primary cell culture.
Specifications
| Applications | Single-cell sequencing, cell culture, or other cell-related detections |
| Compatible Sample Types | Liver, lung, stomach, intestine, kidney, tumor tissue, mammary gland, ovary, fallopian tube, uterus, testis, epididymis, pituitary gland, thyroid gland, thymus gland, esophagus, nasal tissue, mucous membrane, periosteum, skin, lymphoid tissue, embryonic tissue, and soft horn |
| Supported Instruments | Water bath, horizontal centrifuge, cell counter |
| Storage | -20 °C |
| Shelf-life | 24 months |
Kit Components
10 reactions
| Component | 10 Reactions/Kit |
| MTDS | 2 × 1.25 mL |
50 reactions
| Component | 50 Reactions/Kit |
| MTDS | 10 × 1.25 mL |
Product FAQ
1. Q: Is this kit suitable for all mammalian tissues? Are there any clearly unsuitable tissue types?
A: The kit is suitable for various common mammalian tissues, including more than 20 types such as liver, lung, stomach, intestine, kidney, tumor tissue, mammary gland, ovary (as listed in the instruction manual). However, it is not recommended for tissues with extremely high calcification (e.g., mature bone tissue) or excessively high fat content (e.g., omental adipose tissue). The special structure of such tissues may prevent the dissociation solution from acting fully, making it difficult to obtain high-quality single-cell suspensions. If you need to process such tissues, it is recommended to choose a dedicated dissociation kit.
2. Q: The mammalian tissue universal dissociation solution (FG-BA3303-A) in the kit components is 2×1.25 mL, and 10 rxns means 10 experiments can be conducted. What volume of dissociation solution is required for each experiment?
A: Each experiment requires 240 μL of mammalian tissue universal dissociation solution. According to Step 1 of the instruction manual, for each experiment, take one 5 mL centrifuge tube, add 240 μL of dissociation solution and 2760 μL of RPMI 1640 medium, and mix well. The total volume of 2×1.25 mL (2.5 mL in total) of dissociation solution can meet the needs of approximately 10 experiments (2.5 mL = 2500 μL, 2500 μL ÷ 240 μL per experiment ≈ 10.4 experiments). Due to slight losses during operation, it is marked as 10 rxns. If you need to scale up or down the experiment, adjust the volume proportionally.
3. Q: During the digestion process, what are the differences between using a water bath and a hybridization oven? Which equipment is better to choose?
A: The core difference between the two lies in the mixing method: when using a water bath, the centrifuge tube needs to be shaken manually every 3-5 minutes to ensure full contact between the tissue and the dissociation solution; when using a hybridization oven, a rotation speed of 20-30 rpm can be set to achieve automatic mixing, reducing manual operation errors. There is no absolute "better" choice, and it needs to be based on experimental conditions: if the number of experimental samples is large and the operator's time is limited, the automatic mixing of the hybridization oven is more efficient, and it can avoid digestion differences caused by uneven manual shaking strength; if the number of samples is small, manual operation with a water bath can meet the needs. Both types of equipment need to maintain a constant temperature of 37°C.
4. Q: The instruction manual mentions that "different types and states of tissues have different digestion times, and the cell suspension should be quality-checked at regular intervals". How often should the quality check be conducted specifically? What are the core indicators of quality check?
A: It is recommended to conduct the first quality check after 15 minutes of digestion, and then conduct quality checks every 20-30 minutes thereafter. The core indicators of quality check include two aspects: first, cell viability; second, cell dispersion. Take a small amount of suspension for microscopic examination to observe whether there are obvious tissue clumps (if the proportion of tissue clumps is significant, the digestion time can be appropriately extended, but cell viability must be monitored simultaneously to avoid over-digestion).
5. Q: In Steps 5-7, after digestion, it is necessary to filter with a 70 μm cell sieve and wash the centrifuge tube 3 times. Will omitting 1-2 washing steps affect the experimental results?
A: It will significantly affect the results. Omitting washing steps will result in residual dissociation solution, tissue debris, and dead cells not being completely removed: on the one hand, the residual dissociation solution may continue to damage the activity of subsequent cells, especially having a great impact on the cell state in subsequent cell culture or single-cell sequencing; on the other hand, residual impurities will interfere with the quality control results of the cell counter, leading to inaccurate calculation of cell concentration. Therefore, it is necessary to strictly perform 3 washing steps to ensure that a total of 12 mL of filtrate is collected and impurities are fully removed.
6. Q: Steps 9 and 10 mention washing the precipitate twice with PBS containing 5% FBS. What is the function of FBS? Can it be replaced with PBS without FBS?
A: The core function of FBS (fetal bovine serum) is to neutralize the activity of residual dissociation solution, prevent the dissociation solution from continuously damaging cells, and at the same time provide basic nutrients for cells to maintain cell viability. It cannot be replaced with PBS without FBS. If PBS without FBS is used, the residual dissociation solution will continue to damage the cell membrane, leading to a significant decrease in subsequent cell viability. Especially for samples that need to undergo cell culture or single-cell sequencing, this may directly lead to experimental failure.
7. Q: The instruction manual notes that "DMEM medium can replace RPMI 1640 medium". There are differences in their components. Is it necessary to adjust the experimental steps or reagent dosage after replacement?
A: There is no need to adjust the experimental steps or reagent dosage. Both DMEM and RPMI 1640 media are common basic media for mammalian cells. Although there are slight differences in the contents of amino acids, vitamins and other components, both can provide a suitable osmotic pressure and pH environment for tissue dissociation and meet the working conditions of the dissociation solution. When replacing, add it directly according to the original dosage (2760 μL per experiment), which has no significant impact on the dissociation effect and cell viability.
8. Q: If red blood cell removal is required, the red blood cell lysis kit (FG-BA3311) should be used. At which step should this kit be added for operation? What specific points should be noted?
A: It is recommended to perform the red blood cell removal operation after Step 10 and before Step 11. The specific process is: after completing the two PBS washes in Step 10 and discarding the supernatant, add the lysis solution from the red blood cell lysis kit (according to the dosage in the instruction manual of that kit).
9. Q: The kit should be stored at -20°C. If it is accidentally thawed during transportation or before the experiment (e.g., left at room temperature for 1 hour), can the thawed dissociation solution still be used?
A: If it is only thawed briefly (within 1 hour at room temperature and without repeated freezing and thawing), mix it well immediately after thawing and store it back at -20°C, and it can still be used; if the thawing time exceeds 2 hours, or there is repeated freezing and thawing (frozen again after thawing, then thawed again), it cannot be used. The enzyme activity in the dissociation solution is sensitive to temperature. Prolonged storage at room temperature or repeated freezing and thawing will lead to a decrease in enzyme activity and dissociation efficiency, and may result in insufficient tissue digestion. If the thawed dissociation solution has been used, the dissociation effect should be evaluated by shortening the quality check interval and closely monitoring cell viability.
10. Q: Step 13 states that "subsequent experiments should be carried out immediately after quality control". If subsequent experiments cannot be carried out immediately, can the prepared single-cell suspension be stored for a short period? What are the storage conditions?
A: Short-term storage is possible, but the storage time should not exceed 1 hour. The storage condition is a 4°C refrigerator, and repeated shaking should be avoided. During storage, the cell suspension should be adjusted to an appropriate concentration (1×10⁶ - 1×10⁷ cells/mL) with PBS containing 5% FBS, and placed in a sterile low-adhesion centrifuge tube for sealing. It should be noted that if the storage time exceeds 1 hour, cell viability will gradually decrease, especially for single-cell sequencing samples, which may affect the subsequent gene expression detection results; if the suspension needs to be used after storage, it is necessary to re-conduct the cell viability quality check and gently pipette to mix well to avoid cell precipitation.