Product Overview
Overview
FireGene’s Multi Tissue Nuclei Isolation Kit is designed for isolating high-quality nuclei from various animal tissues, enabling efficient single-cell sequencing and chromatin accessibility analysis.
Background Information
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Ideal for gene expression and regulatory mechanism research.
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Preserves cellular integrity by avoiding harsh dissociation.
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Prevents loss of rare or fragile cell types during processing.
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Facilitates freezing and reanalysis of biobanked samples without compromising quality.
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Enhances reproducibility and efficiency across multiple tissue types.
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Compatible with Single Cell ATAC, Multiome ATAC + Gene Expression, and Gene Expression assays.
Detection Principle
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Begins with lysis of cells to break the membrane while retaining nuclear structure.
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Uses density gradient centrifugation to separate nuclei by density.
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Yields purified, intact nuclei ready for downstream applications.
Specifications
| Applications | Single-cell nuclei sequencing |
| Compatible Sample Types | Animal tissue |
| Supported Instruments | Water bath, horizontal centrifuge, cell counter |
| Storage | 4 °C |
| Shelf-life | 12 months |
Kit Components
| Component | 10 Tests/Kit |
| Lysis buffer | 10 mL |
| Front suspension | 15 mL |
| Rear suspension | 50 mL |
| PB1 | 3 mL |
| PB2 | 6 mL |
| PB3 | 6 mL |