FireGene Skin Dissociation Kit for High-Viability Single-Cell Prep

Overview

The FireGene Skin Dissociation Kit is specifically designed for the efficient enzymatic dissociation of skin tissue into high-viability single-cell suspensions. Ideal for single-cell RNA sequencing, flow cytometry, and regenerative studies, this kit is optimized for sensitive skin-derived cells such as keratinocytes, fibroblasts, and immune populations.

Background Information

• Driven by Clinical and Scientific Research Needs:

  • Skin single-cell sequencing is essential to dissect complex cell populations and intercellular signaling.

  • Traditional mechanical or chemical methods often fail to preserve cellular diversity and viability.

  • This kit enables:

    • Identification of keratinocytes, fibroblasts, Langerhans cells, and dermal immune cells.

    • In-depth research into skin disorders like psoriasis, atopic dermatitis, and skin cancer.

    • Discovery of biomarkers and therapeutic targets for dermatological diseases.

    • Analysis of gene expression profiles associated with skin aging, wound healing, and hair follicle regeneration.

• Background of Technological Development:

  • Developed to address the inefficiencies and cell-damaging effects of conventional dissociation methods.

  • Integrates:

    • Advanced enzyme cocktails tailored for complex skin matrix digestion.

    • Precisely calibrated reaction times and temperatures.

    • Carefully balanced enzymatic ratios based on extracellular matrix biology.

  • Results in higher cell recovery rates, improved cell viability, and batch-to-batch consistency.

Detection Principle

• Employs a synergistic enzymatic digestion protocol:

  • Skin samples are finely minced for better enzymatic penetration.

  • A sequence of multiple enzymes is added for progressive matrix breakdown.

  • Reactions are conducted under optimized temperature and timing conditions.

• Process Outcome:

  • Enzymes gradually disrupt extracellular matrix components and intercellular connections.

  • Yields a high-quality single-cell suspension, ideal for downstream analysis such as scRNA-seq, immunophenotyping, or culture assays.

Specifications

Kit Components