| Description |
T4 DNA Polymerase catalyzes the synthesis of DNA in the 5'→3' direction and requires the presence of template and primer. This enzyme has a 3'→5' exonuclease activity which is much more active than that found in DNA Polymerase I (E. coli). Unlike E. coli DNA Polymerase I, T4 DNA Polymerase does not have a 5'→3' exonuclease function. It is applicable to 3' overhang removal to form blunt ends, 5' overhang fill-in to form blunt ends, single strand deletion subcloning, second strand synthesis in site-directed mutagenesis and probe labeling using replacement synthesis. Theoretical protein molecular weight is 104000 daltons. Error Rate: ~ 1x10-6 bases |
| Source |
Purified from a strain of E. coli that carries the T4 DNA Polymerase gene. |
| Applications |
Blunting, PCR, Polymerases for DNA Manipulation |
| Size |
200 U / 1000 U |
| Concentration |
5,000 U/ml |
| Components |
T4 DNA Polymerase (5,000 U/ml); 10X ABuffer B |
| Unit Definition |
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C. |
| Usage |
For Research Use Only |
| Storage |
Storage Conditions: 100 mM KPO4, 1 mM DTT, 50% Glycerol, pH 6.5 @ 25°C. Store the T4 DNA Polymerase at -20°C. Please avoid repeated freeze-thaw cycles. |
