| Description |
T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer and exchange of Pi from the γ position of ATP to the 5' -hydroxyl terminus of polynucleotides (double-and single-stranded DNA and RNA) and nucleoside 3'-monophosphates. T4 Polynucleotide Kinase also catalyzes the removal of 3'-phosphoryl groups from 3'-phosphoryl polynucleotides, deoxynucleoside 3'-monophosphates and deoxynucleoside 3'-diphosphates. T4 PNK is applicable to end-labeling DNA or RNA for probes and DNA sequencing, addition of 5'-phosphates to oligonucleotides to allow subsequent ligation and removal of 3'-phosphoryl groups. |
| Source |
A E. coli strain that carries the cloned T4 Polynucleotide Kinase gene. |
| Applications |
Phosphorylation (Kinase) |
| Size |
250 U / 500 U / 2500 U |
| Concentration |
10,000 U/ml |
| Components |
T4 Polynucleotide Kinase (10,000 U/ml)ï¼›10X T4 PNK Reaction Buffer |
| Unit Definition |
One Richardson unit is defined as the amount of enzyme catalyzing the incorporation of 1 nmol of acid-insoluble [32P] in a total reaction volume of 50 μl in 30 minutes at 37°C in 1X T4 PNK Reaction Buffer with 66 µM [γ-32P] ATP (5 x 106 cpm/µmol) and 0.26 mM 5'-hydroxyl-terminated salmon sperm DNA. |
| Usage |
For Research Use Only |
| Storage |
Store the T4 Polynucleotide Kinase at -20°C. Please avoid repeated freeze-thaw cycles. |
