T7 RNA Polymerase is isolated from recombinant E.coli BL21 strain carrying a plasmid which contains the T7 RNA Polymerase gene without endogenous nucleic acid residues nor endonuclease residues. T7 RNA Polymerase exhibits extremely high specificity to the T7 phage promoter sequences (TAATACGACT CACTATAG) and can be used as the promoter. T7 DNA or DNA cloned downstream from a T7 promoter can serve as a template for T7 RNA Polymerase-directed RNA synthesis. The double-stranded linear blunt-end or the5' overhang DNA can be used as substrate template for T7 RNA Polymerase, hence, linear plasmids and PCR products can be used as templates for in vitro RNA synthesis.
- Generation of templates for in vitro translation.
- Generation of probes for nucleic acid hybridizations.
- Generation of RNA processing substrates.
- Generation of antisense RNA.
- mRNA, sgRNA synthesis
- Radiolabeled RNA probe preparation
In standard reaction, one unit is defined as the amount of enzyme that will incorporate 1 nmol ATP into acid-insoluble material in a total reaction volume of 50 μl in 1 hour at 37°C.
|Component Name||Amount||Storage (°C)|
|T7 RNA Polymerase (50 U/μl)||100 μl||-20|
|5 x T7 Transcription Buffer||1 ml||-20|
For Research Use Only.