Description

The Kinetic Chromogenic Endotoxin Test Kit (TAL Assay) is a reliable and sensitive assay designed for the quantitative determination of bacterial endotoxin levels in human and animal injectable drugs, biological products, and medical devices.

Applications:

This assay is normally used for quantitative determination of bacterial endotoxin content in:

• Human and animal injection drugs
• Biological products
• Medical equipment

Composition:

• Name	Component	Qty.	Spec.
  R1 main reagent	It contains protein complexes, such as C factor, B factor and proclotting enzyme, as well as chromogenic substrate	4 vials	2.6mL
  R2 standard	It contains lipopolysaccharide lyophilized powder; potency calibrated against national standard product	6 vials	See volume on label
  R3 water	Pyrogen-free water	3 vials	8mL
  R4 reconstitution solution	Tris-HCl buffer solution	4 vials	3mL
  R5 microplate	----	1 plate	96 wells

Mechanism:

Bacterial endotoxin can specifically activate factor C in Limulus reagent, activated factor C activates factor B, activated factor B then activates procoagulase, coagulase hydrolyzes the chromogenic substrate in the reaction, and produces free pNA ( P-Nitroaniline) to cause absorbance changes, and the endotoxin concentration is quantified based on the kinetic detection solution absorbance change rate.

Specifications

Methodology	Kinetic Chromogenic Method
Time to Report	~ 90 minutes
Used For	Injection drugs, Biological products, and Medical devices
Storage and Expiry	At 2-8°C the product can be stored for 24 months before opening.
Equipment & Consumables	Microplate reader, vortex shaker, pipette, Endotoxin-free tip are available to purchase. Tecan microplate reader is recommended.

 

Product Q&A

Q: What is the shelf life of the kinetic chromogenic endotoxin testing kit? 

A: The shelf life of the kinetic chromogenic endotoxin testing kit is 2 years from the date of manufacture under recommended storage conditions.

Q: What instruments will be needed for the kinetic chromogenic endotoxin testing kit? 

A: 1. A reader will be needed. This product is compatible with the mainstream readers in the market. 2. A pipette is also needed.

Q: What should I do if I don't have a reader?

A: You can choose our gel clot endotoxin test assay. This product doesn't require a reader. Only simple instruments will be needed, such as a pipette, thermostat, and shaker.

Q: What is the difference between the kinetic chromogenic endotoxin testing kits and gel clot endotoxin testing kit? 

A: Learn the difference between gel clot vs. chromogenic vs. turbidimetric methods.

Q: What should I do if the consumables in the reagent kit are not enough?

A: The consumables we provide are sufficient for 96 tests. If the consumption is high and the supplies are insufficient, you can purchase the Control Standard Endotoxin, pyrogen-free water, pyrogen-free tubes, and pyrogen-free vials separately.