| Description |
MagicScript II Reverse Transcriptase is a recombinant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability. It can be used to synthesize first strand cDNA at higher temperatures than the wild type M-MuLV. The enzyme is active up to 48°C, providing higher specificity, higher yield of cDNA and more full-length cDNA product up to 12 kb. |
| Source |
The gene encoding a mutant M-MuLV Reverse Transcriptase (RNase H-) is expressed in E. coli and purified to near homogeneity. |
| Applications |
cDNA Synthesis, RT-qPCR, RT-PCR and cDNA Synthesis , PCR, Reverse Transcription (cDNA Synthesis), RT-PCR & cDNA Synthesis |
| Size |
4000 U / 10000 U |
| Concentration |
200,000 U/ml |
| Components |
MagicScript II Reverse Transcriptase (200,000 U/ml); 100 mM DTT (10X); 5X First-Strand Buffer |
| Unit Definition |
One unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-insoluble material in a total reaction volume of 50 μl in 10 minutes at 37°C using poly(rA)•oligo(dT)18 as template. |
| Usage |
For Research Use Only |
| Storage |
Storage Conditions: 20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, 0.01% IGEPAL CA-630, pH 7.5 @ 25°C. Store the MagicScript II Reverse Transcriptase at -20°C. Please avoid repeated freeze-thaw cycles. |
