| Description |
T4 DNA Ligase can catalyze the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA and some DNA/RNA hybrids. T4 DNA ligase will seal nicks for these DNA substrates. Applicable to Cloning of restriction fragments, joining linkers and adapters to blunt-ended DNA. |
| Source |
An E.coli strain that carries the T4 DNA ligase gene. |
| Applications |
Cloning Ligation, Adaptor Ligation |
| Size |
80000 U / 400000 U |
| Concentration |
2,000,000 U/ml |
| Components |
T4 DNA Ligase (High Conc.); 10X T4 DNA ligase Reaction Buffer |
| Unit Definition |
One unit is defined as the amount of enzyme required to give 50% ligase of HindIII fragments of λ DNA (5' DNA termini concentration of 0.12 µM, 300- μg/ml) in a total reaction volume of 20 μl in 30 minutes at 16°C in 1X T4 DNA Ligase Reaction Buffer. |
| Usage |
For Research Use Only |
| Storage |
Storage Conditions: 10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH7.4 @ 25°C. Store the T4 DNA Ligase (High Conc.) at -20°C. Please avoid repeated freeze-thaw cycles. |
