Description |
Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5'→ 3' polymerase activity and a 5' flap endonuclease activity. Taq DNA Polymerase was isolated from a recombinant source and offers robust and reliable reactions. It tolerates a wide range of templates and incorporates dUTP, dITP and fluorescently-labeled nucleotides. |
Source |
An E.coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1. |
Applications |
Multiplex PCR, PCR , Specialty PCR, Routine PCR |
Size |
1000 U / 5000 U / 10000 U |
Concentration |
5,000 U/ml |
Components |
Taq DNA Polymerase (5,000 U/ml); 10X PCR Reaction Buffer, Mg2+ plus |
Unit Definition |
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 75°C. |
Usage |
For Research Use Only |
Storage |
Storage Conditions: 10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% Tween 20, 0.5% NP-40, 50% Glycerol, pH 7.4 @ 25°C. Store the Taq DNA Polymerase (Mg2+ Plus Buffer) at -20°C. Please avoid repeated freeze-thaw cycles. |