| Synonym |
Tn5; Tn5 Transposase; Transposase (Tnp) Tn5; Dimer_Tnp_Tn5 |
| Background |
Tn5 transposase is a bacterial enzyme that integrates a DNA fragment into genomic DNA, and is used as a tool for detecting nucleosome-free regions of genomic DNA in eukaryotes. Bacterial Tn5 transposase promotes the transposition of the Tn5 transposon. Tn5 transposase integrates DNA fragments at a specific site in the nucleosomal DNA. In the ATAC-seq method, Tn5 transposase has been employed to insert an adaptor DNA fragment in the nucleosome-free DNA regions and linker DNA regions between nucleosomes. |
| Description |
Magic™ Tn5 Transposase is a naked and hyperactive form of Tn5 transposase. Our Magic Tn5 Transposase can recognize inside, outside and mosaic end sequences of Tn5 transposon. |
| Source |
E.coli |
| Applications |
- Library construction for second-generation sequencing.
- In vitro transgenic experiment.
- ATAC-seq
|
| Size |
25U |
| Concentration |
1 U/μl. |
| Components |
a. Tn5 Transposase(1 U/μl) b. 5x LM Buffer c. 10xTPS Buffer |
| Unit Definition |
One unit Tn5 Transposase is defined as the amount of enzyme that cleaves 1 ug DNA fragment containing recognition seqence in 1 hour at 37°C. |
| Storage Buffer |
50 mM Tris-HCl (pH 8.0), 100 mM NaCl, 0.1 mM EDTA, 0.1% Triton X-100, 50% glycerol (V/V) |
| Usage |
For Research Use Only |
| Storage |
The Tn5 Transposase is shipped with dry ice. Store Tn5 Transposase at -20°C. Avoid repeated freeze-thaw cycles. |
