Tn5 Transposase

FireGeneSKU: FG-TN5BL01

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Product Overview

Synonym Tn5; Tn5 Transposase; Transposase (Tnp) Tn5; Dimer_Tnp_Tn5
Background Tn5 transposase is a bacterial enzyme that integrates a DNA fragment into genomic DNA, and is used as a tool for detecting nucleosome-free regions of genomic DNA in eukaryotes. Bacterial Tn5 transposase promotes the transposition of the Tn5 transposon. Tn5 transposase integrates DNA fragments at a specific site in the nucleosomal DNA. In the ATAC-seq method, Tn5 transposase has been employed to insert an adaptor DNA fragment in the nucleosome-free DNA regions and linker DNA regions between nucleosomes.
Description Magic Tn5 Transposase is a naked and hyperactive form of Tn5 transposase. Our Magic Tn5 Transposase can recognize inside, outside and mosaic end sequences of Tn5 transposon.
Source E.coli
  • Library construction for second-generation sequencing.
  • In vitro transgenic experiment.
  • ATAC-seq
Size 25U
Concentration 1 U/μl.
Components a. Tn5 Transposase(1 U/μl) b. 5x LM Buffer c. 10xTPS Buffer
Unit Definition One unit Tn5 Transposase is defined as the amount of enzyme that cleaves 1 ug DNA fragment containing recognition seqence in 1 hour at 37°C.
Storage Buffer 50 mM Tris-HCl (pH 8.0), 100 mM NaCl, 0.1 mM EDTA, 0.1% Triton X-100, 50% glycerol (V/V)
Usage For Research Use Only
Storage The Tn5 Transposase is shipped with dry ice. Store Tn5 Transposase at -20°C. Avoid repeated freeze-thaw cycles.

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