Description
Overview
The FireGene Animal Tissue Storage Kit is designed to maintain tissue viability and structural integrity during storage. Ideal for applications like single-cell sequencing, organoid culture, and disease modeling, it ensures that tissue samples remain biologically relevant and analytically reliable after preservation.
Background Information
- Supports research and clinical workflows by preserving the physiological condition of tissue samples.
- Enables long-term storage of animal tissues for:
- Single-cell analysis where intact cells are essential.
- Organoid culture requiring high-quality viable tissues.
- Biomarker discovery, drug screening, and histological studies.
- Used in:
- Disease mechanism exploration.
- Toxicity testing in drug development.
- Diagnostic sample preservation in clinical pathology.
Detection Principle
- The solution is formulated to:
- Maintain osmotic and metabolic balance, preventing swelling/shrinkage.
- Include antioxidants to reduce oxidative damage from reactive oxygen species.
- Inhibit apoptotic/necrotic pathways, minimizing cell death during storage.
- The result:
- High-integrity tissue samples ready for downstream applications like scRNA-seq, immunostaining, or culture.
Specifications
| Applications | Single-cell sequencing, cell culture or other cell-related detections |
| Compatible Sample Types | Animal tissue |
| Supported Instruments | / |
| Storage | 4 °C |
| Shelf-life | 24 months |
Kit Components
100ml/kit
| Component | 100 mL/Kit |
| ATS | 100 mL |
500ml/kit
| Component | 500 mL/Kit |
| ATS | 500 mL |
Product FAQ
1. Q1: What is the main purpose of FG-BA3301 Animal Tissue Universal Preservation Solution? And in which experimental scenarios can it be applied?
A: This product provides a simple, safe and effective method for preserving isolated animal tissues. It can well maintain the activity of cells in isolated tissues and solve the problem of preserving and transporting isolated tissue samples after collection. It can be widely used in high-throughput single-cell sequencing and other related scientific research experiments.
It can be used for tissue transportation, preservation, tissue digestion, and subsequently for cell culture, genome and transcriptome extraction, cell differentiation, etc.
2. Q2: What are the product's item number and volume of this preservation solution?
A: Its only component is the animal tissue universal preservation solution, with the item number FG-BA3301 and a volume of 100mL.
3. Q3: How should FG-BA3301 Animal Tissue Universal Preservation Solution be stored? What is its shelf life?
A: It should be stored at -20°C in the dark, with a shelf life of one year. After receiving the product, it should be promptly dispensed into sterile containers of the required volume to avoid repeated freezing and thawing.
4. Q4: What precautions should be taken regarding aseptic operation when using this preservation solution?
A: This product is a sterile product. When using it, you should wear a lab coat and disposable gloves, pay attention to aseptic operation, and avoid microbial contamination to prevent affecting the tissue preservation effect and subsequent experimental results.
5. Q5: Is special treatment required for the preservation solution before use? What is the specific operation?
A: Before use, it is necessary to ensure that the animal tissue preservation solution is completely thawed and mixed uniformly. Moreover, before use, it must be fully inverted and shaken to make the components of the preservation solution completely uniform, ensuring that the concentration and components are consistent in every part. If there is a situation where it cannot be thawed, please contact the manufacturer.
6. Q6: What are the recommendations for cryopreservation tubes and tissue dosage during tissue sampling and dispensing?
A: It is recommended to use 2mL sterile and enzyme-free cryopreservation tubes for dispensing. The amount of fresh tissue stored in each cryopreservation tube is approximately 200mg (about the size of a soybean), and the volume used is 2mL.
7. Q7: Why is it necessary to clean the tissue after obtaining the tissue sample? What is the specific cleaning procedure?
A: Cleaning the tissue is to remove blood, mucus and other types of contaminants on the tissue surface, ensuring the tissue is clean. The specific steps are as follows: quickly place the tissue into an appropriate amount of PBS buffer, gently clamp the tissue with tweezers and stir slowly, gently rinse the tissue surface, and repeat the rinsing until the tissue surface is completely clean with no obvious visible impurities remaining. Also, remove necrotic tissues and pustular tissues.
8. Q8: What is the correct operation process for preserving the cleaned tissue with the preservation solution?
A: Carefully transfer the cleaned tissue into a 2mL cryopreservation tube, quickly fill the cryopreservation tube with the well-mixed animal tissue universal preservation solution to completely immerse the tissue; then tightly wrap the mouth of the cryopreservation tube with parafilm to ensure a tight seal without leakage, and finally place the cryopreservation tube in a low-temperature environment of 2°C~8°C for preservation.
9. Q9: After preserving the tissue with this preservation solution, within how long should subsequent experiments be carried out? How to handle the sample if transportation is needed?
A: After preserving the tissue, subsequent experiments such as cell suspension preparation must be carried out within 48 hours. If transportation is required, the sealed cryopreservation tube can be placed in a suitable foam box, and biological ice packs can be added according to the weather conditions. The principle is to keep the sample in an environment of 2°C~8°C without being frozen, and subsequent experiments should also be carried out within 48 hours.
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